scholarly journals Wilting and inoculation of Lactobacillus buchneri on intercropped triticale-fava silage: effects on nutritive, fermentative and aerobic stability characteristics

2008 ◽  
Vol 19 (4) ◽  
pp. 302 ◽  
Author(s):  
A. MARTÍNEZ-FERNÁNDEZ ◽  
A. SOLDADO ◽  
F. VICENTE

This study investigated the effects of wilting and Lactobacillus buchneri inoculation on fermentation end products, DM recovery, nutritive characteristics and aerobic stability in organically grown triticale-fava bean intercrop silages. For this purpose, a bi-crop of triticale (× Triticosecale Wittm.) and fava bean (Vicia faba L.) was established on an old low-input mixed sward (Lolium perenne-Trifolium repens). The association of triticale and fava bean in winter crops and wilting forages before ensiling improved ensilability characteristics. Wilting for 24 hours before ensiling avoided effluent losses during the fermentation process and reduced ammonia nitrogen production. Inoculation with Lactobacillus buchneri 40788, for a final application rate of 1×105 cfu g-1 of fresh forage ensiled in laboratory silos during 80 days, promoted a higher CP concentration. Furthermore, it promoted changes in the concentration of fermentation end products, decreasing lactic acid and increasing acetic and propionic acids. The effects of Lactobacillus buchneri on aerobic stability were not confirmed in this study. Wilting depressed, but did not inhibit the activity of Lactobacillus buchneri in the fermentation process.;

2021 ◽  
Vol 140 ◽  
pp. 110038
Author(s):  
Inger-Cecilia Mayer Labba ◽  
Hanne Frøkiær ◽  
Ann-Sofie Sandberg
Keyword(s):  

2020 ◽  
Vol 158 (5) ◽  
pp. 438-446
Author(s):  
E. F. S. Faria ◽  
T. C. da Silva ◽  
D. dos S. Pina ◽  
E. M. Santos ◽  
M. L. G. M. L. de Araújo ◽  
...  

AbstractThis study aimed to examine the effects of re-ensiling time and Lactobacillus buchneri on the fermentation profile, chemical composition and aerobic stability of sugarcane silages. The experiment was set up as a repeated measure design consisting of four air-exposure periods (EP)(0, 6, 12, and 24 h) microbial additive (A) (L. buchneri; or lack of there), with five replicates. Sugarcane was ground through a stationary forage chopper and ensiled in four plastic drums of 200-L capacity. After 210 days of storage, the drums were opened and half of the silage mass was treated with L. buchneri at the concentration of 105 cfu/g of forage. Subsequently, the silages were divided into stacks. The re-ensiling process was started immediately, at 0, 6, 12 and 24-hour intervals, by transferring the material to PVC mini-silos. Silos were opened after 120 days of re-ensiling. The use of L. buchneri reduced butyrate concentration but did not change ethanol or acetic acid concentrations and aerobic stability. An interaction effect between L. buchneri and re-ensiling time was observed for dry matter (DM) losses and composition. Lactobacillus buchneri is not effective in improving aerobic stability in re-ensiled sugarcane silages. However, less DM is lost in silages treated with L. buchneri and exposed to air for 24 h. Re-ensiling sugar cane in up to 24 h of exposure to air does not change final product quality.


Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1575
Author(s):  
Fuhou Li ◽  
Zitong Ding ◽  
Adegbola T. Adesogan ◽  
Wencan Ke ◽  
Yun Jiang ◽  
...  

The effects of two strains of class IIa bacteriocin-producing lactic acid bacteria, Lactobacillus delbrueckii F17 and Lactobacillus plantarum (BNCC 336943), or a non-bacteriocin Lactobacillus plantarum MTD/1 (NCIMB 40027), on fermentation quality, microbial counts, and aerobic stability of alfalfa silage were investigated. Alfalfa was harvested at the initial flowering stage, wilted to a dry matter concentration of approximately 32%, and chopped to 1 to 2 cm length. Chopped samples were treated with nothing (control, CON), Lactobacillus delbrueckii F17 (F17), Lactobacillus plantarum (BNCC 336943) (LPB), or Lactobacillus plantarum MTD/1 (NCIMB 40027) (LPN), each at an application rate of 1 × 106 colony-forming units/g of fresh weight. Each treatment was ensiled in quadruplicate in vacuum-sealed polyethylene bags packed with 500 g of fresh alfalfa per bag and ensiled at ambient temperature (25 ± 2 °C) for 3, 7, 14, 30, and 60 days. The samples were then subjected to an aerobic stability test after 60 days of ensiling. Compared with the CON silage, the inoculants reduced the pH after 14 days of ensiling. After 60 days, pH was lowest in the LPB-treated silage, followed by the F17 and LPN-treated silages. Inoculation of F17 increased concentrations of lactic acid in silages fermented for 7, 14, 30, and 60 days relative to other treatments, except for the LPN-treated silages ensiled for 30 and 60 days, in which the lactic acid concentrations were similar to that of F17 silage. Application of F17 and LPB decreased the number of yeast and mold relative to CON and LPN-treated silages. Compared with the CON silage, inoculant-treated silages had greater aerobic stability, water-soluble carbohydrate, and crude protein concentrations, and lower neutral detergent fiber, amino acid nitrogen, and ammonia nitrogen concentrations. The LPB-treated silage had the greatest aerobic stability followed by the F17-treated silage. Both class IIa bacteriocin producing inoculants improved alfalfa silage fermentation quality, reduced the growth of yeasts and molds, and improved the aerobic stability of the ensiled forage to a greater extent than the proven LPN inoculant. However, higher crude protein concentration and lower ammonia nitrogen concentration were observed in LPN-treated silage relative to other treatments.


2018 ◽  
Vol 58 (12) ◽  
pp. 2249 ◽  
Author(s):  
Jipeng Tian ◽  
Risu Na ◽  
Zhu Yu ◽  
Zhongkuan Liu ◽  
Zhenyu Liu ◽  
...  

This study evaluated the effects of lactic acid bacteria inoculants on the fermentation quality and chemical composition of lucerne silage (A), a mixture of lucerne and wheat bran (A+WB), and a mixture of lucerne and corn husk (A+CH). The application rates of wheat bran in A+WB or corn husk in A+CH were 10%, 15%, and 20% of the whole fresh material. These different materials were treated with distilled water (Control), Lactobacillus plantarum 1 (LAB1), L. plantarum 2 (LAB2), L. plantarum 8 (LAB8) or a commercial inoculant (LALMAND) at a rate of 106 CFU/g of fresh forage. As the application rate of the by-products increased, the dry matter, lactate, propionate, and neutral detergent fibre (after heat-stable amylase treatment) contents increased, and the pH and the acetate, ammonia nitrogen, crude protein, and non-fibre carbohydrate contents decreased. The A+WB showed better fermentation quality than A and A+CH. The inoculants had beneficial effects on the silages, but the effects varied. The results indicate that the use of LAB1, LAB2, or LAB8 was better with A, whereas LALMAND was preferable for use with A+WB or A+CH. The saponin content decreased during ensiling and was positively correlated with the pH and the acetate and ammonia nitrogen content but negatively correlated with the dry matter content. The increase in the application rate of by-products and the addition of LALMAND further decreased the saponin content. Overall, the combined effects of the inoculants, by-products, and different application rates improved the fermentation quality and chemical composition and led to greater a reduction of saponin in lucerne silage, but the selection of suitable types and application rates of by-products and inoculants is essential.


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