Long Non-Coding RNA MALAT1 Accelerates Skeletal Muscle Cell Apoptosis and Inflammatory Response in Sepsis Through Decreasing BRCA1 Expression by Recruiting EZH2

This study was undertaken to investigate the antidiabetic and antioxidant of bawang dayak bulbs (Eleutherine palmifolia L., Merr) extract (BDBE) on skeletal muscle cell apoptosis and body weight change in diabetic rat. Single doses alloxan 120 mg/kgBW were administered intraperitoneally to get diabetic rat. Twenty four male Wistar rat of three months old were used in this study. Rat were devided into six groups: negative control group (were not diabetic and treated) (K0), positive control group (were diabetic and treated CMC-Na) (K1), drug control group (were diabetic and administrated metformin as a standard drug) (K2), BDBE dosed 200 (P1), 400 (P2), and 800 mg/kgBW (P3). The treatment was conducted for 14 days. Hypoglycemic effect and body weight measured of all mice was determined at day 7 and 14 of treatment. At the end of research, all of rat were sacrificed and m. gastrocnemius were collected for apoptosis analysis by TUNEL staining and atrophy analysis by Hematoxilin-Eosin staining. The result of this study showed that BDBE decreased skeletal muscle cell apoptosis and mantained the skeletal muscle fiber diameter (atrophy) and body weight change in diabetes rat.

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P3779MRTF-A mediates aortic smooth muscle cell apoptosis and inflammatory response to develop aortic dissection

European Heart Journal ◽

10.1093/eurheartj/ehy563.p3779 ◽

2018 ◽

Vol 39 (suppl_1) ◽

Author(s):

S Ito ◽

H Aoki ◽

M Nishihara ◽

S Ohno ◽

A Furusho ◽

...

Keyword(s):

Smooth Muscle ◽

Inflammatory Response ◽

Aortic Dissection ◽

Smooth Muscle Cell ◽

Muscle Cell ◽

Cell Apoptosis ◽

Aortic Smooth Muscle Cell ◽

Aortic Smooth Muscle ◽

Muscle Cell Apoptosis

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Long non-coding RNA ZFAS1 alleviates sepsis-induced myocardial injury via target miR-34b-5p/SIRT1

Innate Immunity ◽

10.1177/17534259211034221 ◽

2021 ◽

pp. 175342592110342

Author(s):

Dan-Dan Chen ◽

Hong-Wu Wang ◽

Xing-Jun Cai

Keyword(s):

Inflammatory Response ◽

Cell Apoptosis ◽

Myocardial Injury ◽

Luciferase Assay ◽

H9c2 Cell ◽

Mrna Levels ◽

H9c2 Cells ◽

Western Blots ◽

Non Coding Rna ◽

Long Non Coding Rna

Long non-coding RNA ZFAS1 is down-regulated in sepsis. However, whether ZFAS1 participates in sepsis-induced cardiomyopathy (SIC) remains largely unknown. LPS injection to rats was used to establish an in vivo sepsis model, while LPS stimulation with H9C2 cell was used to mimic an in vitro sepsis-induced myocardial injury model. Western blots and quantitative RT-PCR were performed to evaluate protein and mRNA levels, respectively. ELISA was conducted to determine cytokine levels in supernatant. Flow cytometry was used to test apoptosis. Dual-luciferase assay was performed to validate binding between ZFAS1 and miR-34b-5p, miR-34b-5p and SIRT1. Our data revealed that ZFAS1 and SIRT1 were down-regulated, while miR-34b-5p was up-regulated in LPS-induced H9C2 cells. Inhibition of miR-34b-5p or overexpression of ZFAS1 alleviated inflammatory response and cell apoptosis in LPS-stimulated H9C2 cells. A mechanism study revealed that ZFAS1 sponged miR-34b-5p and thus elevated expression of SIRT1, which was prohibited by miR-34b-5p. ZFAS1 alleviated inflammatory response and cell apoptosis in LPS-stimulated H9C2 cells via the miR-34b-5p/SIRT1 axis, providing novel potential therapeutic targets for SIC.

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