scholarly journals Mechano-signalling, induced by fullerene C60 nanofilms, arrests the cell cycle in the G2/M phase and decreases proliferation of liver cancer cells

2019 ◽  
Vol Volume 14 ◽  
pp. 6197-6215 ◽  
Author(s):  
Malwina Sosnowska ◽  
Marta Kutwin ◽  
Sławomir Jaworski ◽  
Barbara Strojny ◽  
Mateusz Wierzbicki ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Liver Cancer Cells ◽  
M Phase

Titanium Oxide Nanoparticles Improve the Chemotherapeutic Action of Erlotinib in Liver Cancer Cells

2020 ◽  
Vol 16 (4) ◽  
pp. 337-343
Author(s):  
Shaimaa E. Abdel-Ghany ◽  
Eman El-Sayed ◽  
Nour Ashraf ◽  
Nada Mokhtar ◽  
Amany Alqosaibi ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Titanium Oxide ◽  
Phase Distribution ◽  
Oxide Nanoparticles ◽  
Titanium Oxide Nanoparticles ◽  
Liver Cancer Cells ◽  
M Phase ◽  
Apoptosis Detection ◽  
Novel Method

Background: Hepatocellular carcinoma is the second leading cause of cancer-related deaths among other types of cancer due to lack of effective treatments and late diagnosis. Nanocarriers represent a novel method to deliver chemotherapeutic drugs, enhancing their bioavailability and stability. Methods: In the present study, we loaded gold nanoparticles (AuNPs) and titanium oxide nanoparticles (TiO2NPs) with ERL to investigate the efficiency of the formed composite in inducing apoptosis in HepG2 liver cancer cells. Cytotoxicity was assessed using MTT assay and cell phase distribution was assessed by flow cytometry along with apoptosis detection. Results: Data obtained indicated the efficiency of the formed composite to significantly induce cell death and arrest cell cycle and G2/M phase. IRF4 was downregulated after treatment with loaded ERL. Conclusion: Our data showed that loading ERL on TiO2NPs was more efficient than AuNPs. However, both nanocarriers were efficient compared with control.

A resveratrol analog, phoyunbene B, induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells

2012 ◽  
Vol 22 (5) ◽  
pp. 2114-2118 ◽  
Author(s):  
Guanghui Wang ◽  
Xiaoyu Guo ◽  
Haifeng Chen ◽  
Ting Lin ◽  
Yang Xu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Liver Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
M Cell ◽  
Liver Cancer Cells ◽  
Cycle Arrest

scholarly journals NU7441 Enhances the Radiosensitivity of Liver Cancer Cells

2016 ◽  
Vol 38 (5) ◽  
pp. 1897-1905 ◽  
Author(s):  
Chuanjie Yang ◽  
Quanxu Wang ◽  
Xiaodan Liu ◽  
Xiulian Cheng ◽  
Xiaoyu Jiang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Dna Repair ◽  
Liver Cancer ◽  
Protein Expression ◽  
Cancer Cells ◽  
Hepg2 Cells ◽  
Specific Inhibitor ◽  
Cell Counting ◽  
Liver Cancer Cells

Objective: Radiation therapy, one of the major treatments for liver cancer, causes DNA damage and cell death. Since the liver cancer cells have a strong capacity to repair irradiative injury, new medicines to enhance this treatment are urgently required. In this study, we investigated the effect of NU7441, a synthetic small-molecule compound, as a specific inhibitor of DNA-dependent protein kinase (DNA-PK) in radiosensitization of hepatocellular carcinoma HepG2 cells. Methods: Cell Counting Kit-8 (CCK-8) was first used to evaluate the proliferation of HepG2 cells under NU7441 treatment. SDS-PAGE and Western blot were then performed to study the protein expression leading to the DNA damage repair. Further, neutral single cell gel electrophoresis and immunofluorescence assay were carried out to assess DNA repair. Finally, flow cytometry was implemented to examine the changes in cell cycle. Results: NU7441 reduced the CCK-8 counts in the HepG2 culture, further enhanced 60Coγ radiation injury to HepG2 cells, which was manifested by decreasing the DNA-PKcs (S2056) protein expression, increasing γH2AX foci number, prolonging the tail moment of the comet cells, and inducing cell cycle arrest at G2/M phase. Conclusion: NU7441 inhibited the growth of liver cancer cells, enhanced the radiosensitization of these cancer cells by interfering with the DNA repair and cell cycle checkpoint. These data implicate NU7441 as a potential radiotherapy sensitizer for the treatment of liver cancer.

Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells

2006 ◽  
Vol 241 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Guanghui Wang ◽  
Haifeng Chen ◽  
Minghui Huang ◽  
Naili Wang ◽  
Jinchao Zhang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Liver Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
M Cell ◽  
Liver Cancer Cells ◽  
Cycle Arrest

scholarly journals Histone deacetylase inhibitor MGCD0103 causes cell cycle arrest, apoptosis, and autophagy in liver cancer cells

2020 ◽  
Vol 11 (7) ◽  
pp. 1915-1926 ◽  
Author(s):  
Bo Liao ◽  
Quan Sun ◽  
Yufeng Yuan ◽  
Yuchun Yin ◽  
Jianguo Qiao ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Liver Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Liver Cancer Cells ◽  
Cycle Arrest ◽  
Deacetylase Inhibitor

scholarly journals Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells

2016 ◽  
Vol 13 (3) ◽  
pp. 2261-2266 ◽  
Author(s):  
SHENG-QIAN XU ◽  
YONG QIN ◽  
DE-BIAO PAN ◽  
GUAN-XIONG YE ◽  
CHENG-JUN WU ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Liver Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Liver Cancer Cells ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest

scholarly journals Acanthopanax senticosus Harms extract causes G0/G1 cell cycle arrest and autophagy via inhibition of Rubicon in human liver cancer cells

2021 ◽  
Vol 45 (3) ◽  
pp. 1193-1201
Author(s):  
Yutaka Kawano ◽  
Maki Tanaka ◽  
Masaki Fujishima ◽  
Eri Okumura ◽  
Hideo Takekoshi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Acanthopanax Senticosus ◽  
Liver Cancer Cells ◽  
Cycle Arrest ◽  
Human Liver Cancer ◽  
G1 Cell Cycle Arrest ◽  
Human Liver Cancer Cells

Preparation of Polyethylene Glycol-Poly Lactic Acid Water-Soluble Nano-Particles and Its Effect on the Proliferation, Metastasis, and Angiogenesis of Liver Cancer Cells

2021 ◽  
Vol 13 (8) ◽  
pp. 1547-1556
Author(s):  
Juan He ◽  
Song Li ◽  
Junli Liu
Keyword(s):  
Cell Cycle ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Water Soluble ◽  
Nano Particles ◽  
Poly Lactic Acid ◽  
Dependent Manner ◽  
Liver Cancer Cells ◽  
Cell Metastasis

The onset of the disease is secretive, and the accuracy of early diagnosis is not high. Patients will lose the opportunity of surgical treatment because of severe liver cirrhosis and cancer cell metastasis. Vanib nano-drug was prepared in this study by a polymer, which was used for in vitro research of liver cancer cells. First, polyethylene glycol (PEG)-poly lactic acid (PLA) and Vanib were dissolved in acetone to form a mixed solution, and then, water-soluble nano-particles were obtained by ultrasonic and deionized water. Then, the nano-particles were modified by iRGD and characterized by electron microscopy, DLS, and in vitro release. Besides, CCK-8, flow cytometry apoptosis test, flow cytometry cell cycle test, Transwell cell invasion and metastasis test, and angiogenesis test were applied to detect the effect of the nano-drug on the proliferation, cell metastasis, and angiogenesis of liver cancer cells. The results showed that Vanib nano-particles inhibited the proliferation of liver cancer cells in a dose-dependent manner, and induced apoptosis to G1 block. What’s more, Vanib nano-particles reduced cell viability in a Caspase-dependent manner, thereby inducing cell death. The Vanib nano-particles up-regulated the expression of P21 and also inhibited the cyclins Cyclin E2 and Cycllin B, thereby blocking the cell cycle process. In addition, Vanib nano-particles and free Vanib could inhibit the activity of human umbilical vein endothelial cells (HUVECs) and angiogenesis, which proved that the prepared Vanib nano-particles could effectively curb the growth of liver cancer cells.

Sign in / Sign up

Export Citation Format

Share Document