Titanium Oxide Nanoparticles Improve the Chemotherapeutic Action of Erlotinib in Liver Cancer Cells

Background: Hepatocellular carcinoma is the second leading cause of cancer-related deaths among other types of cancer due to lack of effective treatments and late diagnosis. Nanocarriers represent a novel method to deliver chemotherapeutic drugs, enhancing their bioavailability and stability. Methods: In the present study, we loaded gold nanoparticles (AuNPs) and titanium oxide nanoparticles (TiO2NPs) with ERL to investigate the efficiency of the formed composite in inducing apoptosis in HepG2 liver cancer cells. Cytotoxicity was assessed using MTT assay and cell phase distribution was assessed by flow cytometry along with apoptosis detection. Results: Data obtained indicated the efficiency of the formed composite to significantly induce cell death and arrest cell cycle and G2/M phase. IRF4 was downregulated after treatment with loaded ERL. Conclusion: Our data showed that loading ERL on TiO2NPs was more efficient than AuNPs. However, both nanocarriers were efficient compared with control.

Download Full-text

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Cancers ◽

10.3390/cancers11121873 ◽

2019 ◽

Vol 11 (12) ◽

pp. 1873 ◽

Cited By ~ 7

Author(s):

Oleg Lunov ◽

Mariia Uzhytchak ◽

Barbora Smolková ◽

Mariia Lunova ◽

Milan Jirsa ◽

...

Keyword(s):

Iron Oxide ◽

Liver Cancer ◽

Cancer Cells ◽

Iron Oxide Nanoparticles ◽

Magnetic System ◽

Short Pulse ◽

Magnetic Nanomaterials ◽

Oxide Nanoparticles ◽

Liver Cancer Cells ◽

Wide Range

Lysosome-activated apoptosis represents an alternative method of overcoming tumor resistance compared to traditional forms of treatment. Pulsed magnetic fields open a new avenue for controlled and targeted initiation of lysosomal permeabilization in cancer cells via mechanical actuation of magnetic nanomaterials. In this study we used a noninvasive tool; namely, a benchtop pulsed magnetic system, which enabled remote activation of apoptosis in liver cancer cells. The magnetic system we designed represents a platform that can be used in a wide range of biomedical applications. We show that liver cancer cells can be loaded with superparamagnetic iron oxide nanoparticles (SPIONs). SPIONs retained in lysosomal compartments can be effectively actuated with a high intensity (up to 8 T), short pulse width (~15 µs), pulsed magnetic field (PMF), resulting in lysosomal membrane permeabilization (LMP) in cancer cells. We revealed that SPION-loaded lysosomes undergo LMP by assessing an increase in the cytosolic activity of the lysosomal cathepsin B. The extent of cell death induced by LMP correlated with the accumulation of reactive oxygen species in cells. LMP was achieved for estimated forces of 700 pN and higher. Furthermore, we validated our approach on a three-dimensional cellular culture model to be able to mimic in vivo conditions. Overall, our results show that PMF treatment of SPION-loaded lysosomes can be utilized as a noninvasive tool to remotely induce apoptosis.

Download Full-text

Novel titanium oxide nanoparticles for effective delivery of paclitaxel to human breast cancer cells

Journal of Nanoparticle Research ◽

10.1007/s11051-014-2739-x ◽

2014 ◽

Vol 16 (12) ◽

Cited By ~ 2

Author(s):

R. Mund ◽

N. Panda ◽

S. Nimesh ◽

A. Biswas

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Titanium Oxide ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Oxide Nanoparticles ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Titanium Oxide Nanoparticles ◽

Effective Delivery

Download Full-text

Aurora kinase inhibitors attached to iron oxide nanoparticles enhances inhibition of the growth of liver cancer cells

Journal of Nanoparticle Research ◽

10.1007/s11051-014-2708-4 ◽

2015 ◽

Vol 17 (6) ◽

Cited By ~ 1

Author(s):

Xiquan Zhang ◽

Li Xie ◽

Ming Zheng ◽

Juan Yao ◽

Lina Song ◽

...

Keyword(s):

Iron Oxide ◽

Liver Cancer ◽

Cancer Cells ◽

Iron Oxide Nanoparticles ◽

Kinase Inhibitors ◽

Aurora Kinase ◽

Oxide Nanoparticles ◽

Liver Cancer Cells ◽

Aurora Kinase Inhibitors

Download Full-text

Study of zinc oxide nanoparticles on epithelial-mesenchymal transition and toxicity of spontaneous monocyte-mediated cytotoxity-7721 cells

Materials Express ◽

10.1166/mex.2021.1986 ◽

2021 ◽

Vol 11 (6) ◽

pp. 832-838

Author(s):

Luoluo Wang ◽

Yi Ruan ◽

Xiang Wu ◽

Xinhua Zhou

Keyword(s):

Liver Cancer ◽

Cancer Cells ◽

Exposure Time ◽

Zinc Oxide Nanoparticles ◽

Epithelial Mesenchymal Transition ◽

Oxide Nanoparticles ◽

Zno Nanoparticle ◽

Nanoparticle Concentration ◽

Mesenchymal Transition ◽

Liver Cancer Cells

To explore the method of studying zinc oxide nanoparticles on the epithelial-mesenchymal transition and toxicity of liver cancer SMMC-7721 cells. Human liver cancer SMMC-7721 cells is stored in 90% FBS+10% DMSO liquid nitrogen. ZnO suspension was prepared, cell viability was assessed using MTT assay, cell apoptosis was analyzed by flow cytometry, and EMT-related proteins were detected by western blotting. Results showed LDH activity increased continuously with the increase in ZnO nanoparticle concentration and exposure time (P < 0.001). Both ATP and SOD activities gradually decreased with the increase in ZnO nanoparticle concentration and exposure time (both P < 0.001). The MTT assay revealed that with the increase in ZnO dose, the proliferation of SMMC-7721 liver cancer cells decreased gradually (P < 0.001), and with the continuous increase in exposure time to ZnO nanoparticles, the reproductive viability of these cells also continued to decline (P < 0.001). The apoptosis rate of SMMC-7721 liver cancer cells increased with the increase in ZnO dose (P < 0.001). Flow cytometry results demonstrated that the apoptosis rate of SMMC-7721 liver cancer cells increased with the continuous prolongation of treatment time (P < 0.001). Western blotting experiments revealed that the concentrations of vimentin, Snail, N-cadherin, and Slugn proteins in SMMC-7721 cells increased significantly, whereas those of E-cadherin and ZO-1 decreased significantly, with the increase in ZnO dose (both P < 0.001). Therefore, ZnO nanoparticles can induce apoptosis of SMMC-7721 liver cancer cells, inhibit cell proliferation and EMT, and can be used as a new nanoparticle carrier for potential treatment of liver cancer.

Download Full-text