scholarly journals Impact of Murraya koenigii (L.) Sprengel (Rutaceae) extracts on lifespan and oxidative stress markers in Drosophila melanogaster Meigen, 1830 (Diptera)

2020 ◽  
Vol 7 (15) ◽  
pp. 59-67
Author(s):  
Dinesh Kumar ◽  
Sunita Sunita ◽  
Veer Bhan
Keyword(s):  
Oxidative Stress ◽  
Drosophila Melanogaster ◽  
Free Radicals ◽  
Antioxidant Enzyme ◽  
Enzyme System ◽  
Antioxidant Properties ◽  
Fruit Fly ◽  
Standard Diet ◽  
Murraya Koenigii ◽  
Antioxidant Enzyme System

The free radicals (ROS and RNS) damage to proteins, DNA, lipids of the cell. These free radicals creates the imbalance in physiological functions and acts as a prevalent cause of various diseases such as cancer, diabetes, cardiovascular diseases, aging, oxidative stress and metabolic syndrome by dysfunction of antioxidant enzyme system of cell. Using the fruit fly Drosophila melanogaster Meigen, 1830 (Diptera) as a model we examined the antioxidant properties of Murraya koenigii (L.) Sprengel (Rutaceae) on the life history parameters. We demonstrate a novel physiological interaction between free radicals, oxidative stress and antioxidant enzyme system by using extracts of M. koenigii in standard diet of the fly. This study describes how this interaction impacts a very early cellular defect associated with ageing and ageing associate diseases. We also describe progressive deficits in flies expressing the superoxide dismutase gene, catalase and lipid peroxidation. Collectively, our work demonstrates that Drosophila can be used to study the cellular, physiological and behavioral basis of human ageing related diseases.

scholarly journals Nutraceutical Strategy to Counteract Eye Neurodegeneration and Oxidative Stress in Drosophila melanogaster Fed with High-Sugar Diet

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1197
Author(s):  
Elisabetta Catalani ◽  
Giuseppina Fanelli ◽  
Federica Silvestri ◽  
Agnese Cherubini ◽  
Simona Del Quondam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drosophila Melanogaster ◽  
Antioxidant Properties ◽  
Fruit Fly ◽  
Retinal Cell ◽  
Food Supplementation ◽  
In Vivo Model ◽  
Retinal Neurons ◽  
High Sucrose

Aberrant production of reactive oxygen species (ROS) is a common feature of damaged retinal neurons in diabetic retinopathy, and antioxidants may exert both preventive and therapeutic action. To evaluate the beneficial and antioxidant properties of food supplementation with Lisosan G, a powder of bran and germ of grain (Triticum aestivum) obtained by fermentation with selected lactobacillus and natural yeast strains, we used an in vivo model of hyperglycemia-induced retinal damage, the fruit fly Drosophila melanogaster fed with high-sucrose diet. Lisosan G positively affected the visual system of hyperglycemic flies at structural/functional level, decreased apoptosis, and reactivated protective autophagy at the retina internal network. Also, in high sucrose-fed Drosophila, Lisosan G reduced the levels of brain ROS and retina peroxynitrite. The analysis of oxidative stress-related metabolites suggested 7,8-dihydrofolate, uric acid, dihydroorotate, γ-L-glutamyl-L-cysteine, allantoin, cysteinyl-glycine, and quinolate as key mediators of Lisosan G-induced inhibition of neuronal ROS, along with the upregulation of glutathione system. Of note, Lisosan G may impact oxidative stress and the ensuing retinal cell death, also independently from autophagy, although the autophagy-ROS cross-talk is critical. This study demonstrated that the continuous supplementation with the alimentary integrator Lisosan G exerts a robust and multifaceted antioxidant effect on retinal neurons, thus providing efficacious neuroprotection of hyperglycemic eye.

scholarly journals AGE-RELATED CHANGES OF RELIABILITY OF ANTIOXIDANT ENZYME DEFENSE ARE ASSOCIATED WITH ADAPTIVE BEHAVIOR OF PRIMATES

2012 ◽  
Vol 10 (2) ◽  
pp. 20-27
Author(s):  
Nadezda Dmitrievna Goncharova ◽  
Viktor Yuryevich Marenin ◽  
Arsen Arsenovich Vengerin ◽  
Alla Vyacheslavovna Shmaliy
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Enzyme ◽  
Adaptive Behavior ◽  
Rhesus Monkeys ◽  
Enzyme System ◽  
Antioxidant Enzyme System ◽  
Age Related ◽  
Female Rhesus ◽  
Age Related Changes

We have investigated age-related changes in the activity of antioxidant enzyme system and intensity of lipid peroxidation in erythrocytes of young and old female rhesus monkeys with depression-like and standard behavior. Revealed significant disturbances in the activities of GR and GSH-Px in monkeys with depression-like behavior are accompanied more pronounced age-related decrease in reliability of antioxidant enzyme defense and development of oxidative stress.

scholarly journals Changes in Antioxidative, Oxidoreductive and Detoxification Enzymes during Development of Aphids and Temperature Increase

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1181
Author(s):  
Roma Durak ◽  
Jan Dampc ◽  
Monika Kula-Maximenko ◽  
Mateusz Mołoń ◽  
Tomasz Durak
Keyword(s):  
Antioxidant Enzymes ◽  
Enzymatic Activity ◽  
Antioxidant Enzyme ◽  
Temperature Increase ◽  
Enzyme System ◽  
Life Cycles ◽  
Detoxification Enzymes ◽  
Strong Positive Correlation ◽  
Positive Correlation ◽  
Antioxidant Enzyme System

Temperature, being the main factor that has an influence on insects, causes changes in their development, reproduction, winter survival, life cycles, migration timing, and population dynamics. The effects of stress caused by a temperature increase on insects may depend on many factors, such as the frequency, amplitude, duration of the stress, sex, or the developmental stage of the insect. The aim of the study was to determine the differences in the enzymatic activity of nymphs and adult aphids Aphis pomi, Macrosiphum rosae and Cinara cupressi, and changes in their response to a temperature increase from 20 to 28 °C. The activity of enzymatic markers (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), β-glucosidase, polyphenol oxidase (PPO) and peroxidase (POD)) in aphid tissues was analysed for three constant temperatures. The results of our research showed that the enzymatic activity of aphids (measured as the activity of antioxidant, detoxifying and oxidoreductive enzymes) was mainly determined by the type of morph. We observed a strong positive correlation between the activity of the detoxifying and oxidoreductive enzymes and aphids’ development, and a negative correlation between the activity of the antioxidant enzymes and aphids’ development. Moreover, the study showed that an increase in temperature caused changes in enzyme activity (especially SOD, CAT and β-glucosidase), which was highest at 28 °C, in both nymphs and adults. Additionally, a strong positive correlation between metabolic activity (heat flow measured by microcalorimeter) and longevity was observed, which confirmed the relationship between these characteristics of aphids. The antioxidant enzyme system is more efficient in aphid nymphs, and during aphid development the activity of antioxidant enzymes decreases. The antioxidant enzyme system in aphids appears to deliver effective protection for nymphs and adults under stressful conditions, such as high temperatures.

scholarly journals Increased Tolerance of Citrus (Citrus tangerina) Seedlings to Soil Water Deficit after Mycorrhizal Inoculation: Changes in Antioxidant Enzyme Defense System

2013 ◽  
Vol 41 (2) ◽  
pp. 524 ◽  
Author(s):  
Qiu-Dan NI ◽  
Ying-Ning ZOU ◽  
Qiang-Sheng WU ◽  
Yong-Ming HUANG
Keyword(s):  
Antioxidant Enzyme ◽  
Soil Water ◽  
Water Deficit ◽  
Mycorrhizal Fungi ◽  
Enzyme System ◽  
Arbuscular Mycorrhizal ◽  
Temporal Variations ◽  
Soil Drying ◽  
Soil Water Deficit ◽  
Antioxidant Enzyme System

Arbuscular mycorrhizal fungi (AMF) can enhance tolerance of plants to soil water deficit, whereas morphological observations of reactive oxygen species and antioxidant enzyme system are poorly studied. The present study thereby evaluated temporal variations of the antioxidant enzyme system in citrus (Citrus tangerina) seedlings colonized by Glomus etunicatum and G. mosseae over a 12-day period of soil drying. Root colonization by G. etunicatum and G. mosseae decreased with soil drying days from 32.0 to 1.0% and 50.1 to 4.5% in 0-day to 12-day, respectively. Compared to the non-AM controls, the AMF colonized plants had significantly lower tissue (both leaves and roots) hydrogen peroxide (H2O2) and superoxide anion radical (O2•–) concentrations during soil water deficit, whereas 1.03–1.92, 1.25–1.84 and 1.18–1.69 times higher enzyme activity in superoxide dismutase, peroxidase (POD) and catalase. In situ leaf H2O2 and root POD location also showed that AM seedlings had less leaf H2O2 but higher root POD accumulation. Furthermore, significantly higher root infection and antioxidant enzymatic activities in plants colonized with G. mosseae expressed than with G. etunicatum during the soil drying. These results demonstrated that the AMs could confer greater tolerance of citrus seedlings to soil water deficit through an enhancement in their antioxidant enzyme defence system whilst an decrease level in H2O2 and O2•–.

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