scholarly journals Overexpression of the GmDREB2 gene increases proline accumulation and tolerance to drought stress in soybean plants

2020 ◽  
pp. 495-503
Author(s):  
Thi Thanh Nhan Pham ◽  
Huu Quan Nguyen ◽  
Thi Ngoc Lan Nguyen ◽  
Xuan Tan Dao ◽  
Danh Thuong Sy ◽  
...  
Keyword(s):  
Glycine Max ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Proline Accumulation ◽  
Stress Conditions ◽  
Transgenic Soybean ◽  
Transcription Level ◽  
Drought Tolerant ◽  
Transgenic Lines ◽  
Soybean Plants

The dehydration responsive element binding (DREB) is a plant protein subfamily expressed when soybean plants face abiotic stresses. These DREB proteins are also considered to activate the transcription of drought-resistant genes. In this study, we present the determined results of relationships between overexpression of Glycine max DREB2 (GmDREB2) with the transcription level of Glycine max pyrroline-5-carboxylate synthetase (GmP5CS) gene, proline accumulation and drought tolerant ability transgenic soybean plants as the basis for selection of transgenic lines with high drought tolerance. GmDREB2 was inserted into a plant transgenic vector and the 35S-GmDREB2-cmyc construct was transferred into the soybean plants by Agrobacterium-mediated transformation. Recombinant GmDREB2 protein with a molecular weight of approximately 20 kDa was expressed in four transgenic soybean lines in the T1 generation. The GmP5CS gene was shown to have significantly (P<0.05) increased expression in the T2 transgenic soybean lines and higher than compared to non-transgenic plants with considering both in well watered condition and stressed conditions were from 1.06 to 1.31-fold and 1.37 to 1.95-fold, respectively. The proline content of four transgenic soybean lines increased 155.81% to 187.42 % after five days in drought-stress conditions and increased from 180.52 % to 233.74 % after nine days under drought-stress conditions (P<0.05). Therefore, the overexpression of GmDREB2 resulted in increasing transcription level of P5CS gene, proline accumulation and drought-stress tolerance of the transgenic soybean plants. The GmDREB2 transformation into soybean plants was confirmed by the results of genetically modified lines in the T2 generation (T2-1, T2-6, T2-7, and T2-8) with higher drought tolerant ability than those of non-transgenic plants.

scholarly journals Effect of Drought Stress at Supraoptimal Temperature on Polyamine Concentrations in Transgenic Soybean with Increased Proline Levels

2006 ◽  
Vol 61 (11-12) ◽  
pp. 833-839 ◽  
Author(s):  
Livia Simon-Sarkadi ◽  
Gábor Kocsy ◽  
Ágnes Várhegyi ◽  
Gábor Galiba ◽  
Jacoba A. de Ronde
Keyword(s):  
Glycine Max ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Genetic Manipulation ◽  
Free Proline ◽  
Proline Biosynthesis ◽  
Wild Type ◽  
Transgenic Soybean

Abstract The effect of drought stress at supraoptimal temperature on free proline and polyamine levels was compared in wild type and transgenic soybean (Glycine max cv. Ibis) plants having increased proline levels. Since glutamate and arginine are precursors of both proline and polyamines, it was assumed that the genetic manipulation of proline levels would also affect the polyamine levels. The proline and spermine concentrations increased, while the putrescine concentration generally decreased or did not change after the treatments in both genotypes. Following drought higher proline and lower spermine levels were detected in the transgenic plants compared to the wild type ones, which could be explained by the increased use of their common precursors for proline biosynthesis in the transgenic plants.

scholarly journals Biochemical examination of non-transgenic and transgenic soybean plants under drought stress conditions

2019 ◽  
Vol 63 (1) ◽  
pp. 314-322
Author(s):  
C.G. Goncalves ◽  
A.C. Silva ◽  
L.R. Alves ◽  
M.R.R. Pereira ◽  
P.L. Gratao ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Conditions ◽  
Transgenic Soybean ◽  
Soybean Plants ◽  
Biochemical Examination

scholarly journals Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mahmoud M. Gaballah ◽  
Azza M. Metwally ◽  
Milan Skalicky ◽  
Mohamed M. Hassan ◽  
Marian Brestic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Ssr Markers ◽  
Similarity Index ◽  
Stress Conditions ◽  
Yield Potential ◽  
Drought Tolerant ◽  
Rice Genotypes

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.

Photosynthetic response of transgenic soybean plants, containing an Arabidopsis P5CR gene, during heat and drought stress

2004 ◽  
Vol 161 (11) ◽  
pp. 1211-1224 ◽  
Author(s):  
J.A. De Ronde ◽  
W.A. Cress ◽  
G.H.J. Krüger ◽  
R.J. Strasser ◽  
J. Van Staden
Keyword(s):  
Drought Stress ◽  
Photosynthetic Response ◽  
Transgenic Soybean ◽  
Soybean Plants

Proline accumulation and glutathione reductase activity induced by drought-tolerant rhizobacteria as potential mechanisms to alleviate drought stress in Guinea grass

2020 ◽  
Vol 147 ◽  
pp. 103367 ◽  
Author(s):  
Andres Eduardo Moreno-Galván ◽  
Sandra Cortés-Patiño ◽  
Felipe Romero-Perdomo ◽  
Daniel Uribe-Vélez ◽  
Yoav Bashan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Proline Accumulation ◽  
Drought Tolerant ◽  
Guinea Grass ◽  
Glutathione Reductase Activity

scholarly journals Phenological, Physiological and yield markers as efficient tools to identify drought tolerant rice genotypes in Eastern India

2020 ◽  
Author(s):  
Soumya Kumar Sahoo ◽  
Goutam Kumar Dash ◽  
Arti Guhey ◽  
Mirza Jaynul Baig ◽  
Madhusmita Barik ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Grain Yield ◽  
Stress Conditions ◽  
Eastern Region ◽  
Eastern India ◽  
Drought Tolerant ◽  
Significant Difference ◽  
Rice Genotypes ◽  
Highly Correlated

ABSTRACTRice production is severely threatened by drought stress in Eastern India. To develop drought tolerant varieties, selection of donors for breeding programme is crucial. Twenty one selected rice genotypes including both tolerant and sensitive to drought were grown under well-watered and drought stress conditions in dry seasons of two successive years of 2017 and 2018. Leaf water potential, relative water content displayed significant difference among the genotypes during vegetative screening. At reproductive stage drought screening, days to 50% flowering was delayed in all genotypes except N22 and Anjali (showed early flowering) however grain yield and other yield related traits decreased significantly compared to well watered condition. Correlation analysis of phenological and yield related traits with grain yield revealed that tiller numbers and panicle numbers are highly correlated with grain yield both under well-watered and water stress conditions and contributes maximum towards grain yield. The dendrogram grouped Mahamaya, Sahabhagidhan, Poornima, IBD 1, Hazaridhan, Samleshwari and Danteshwari into one cluster which performed better under water stress conditions and had grain yield more than 1.69 tha−1. Sahabhagidhan, Poornima, Vandana, and N22 displayed tolerance to drought both under vegetative and reproductive conditions which could be a good selection for the breeders to develop drought tolerant rice cultivars for eastern region of India.

scholarly journals Protein Profiles and Dehydrin Accumulation in Some Soybean Varieties (<i>Glycine max</i> L. Merr) in Drought Stress Conditions

2013 ◽  
Vol 04 (01) ◽  
pp. 134-141 ◽  
Author(s):  
Estri Laras Arumingtyas ◽  
Evika Sandi Savitri ◽  
Runik Dyah Purwoningrahayu
Keyword(s):  
Glycine Max ◽  
Drought Stress ◽  
Stress Conditions ◽  
Protein Profiles

scholarly journals Soybean plants expressing the Bacillus thuringiensis cry8-like gene show resistance to Holotrichia parallela

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Di Qin ◽  
Xiao-Yi Liu ◽  
Cristina Miceli ◽  
Qi Zhang ◽  
Pi-wu Wang
Keyword(s):  
Bacillus Thuringiensis ◽  
Transgenic Plants ◽  
Survival Rates ◽  
Pcr Analysis ◽  
Transgenic Soybean ◽  
Cry Protein ◽  
Transgenic Expression ◽  
Holotrichia Parallela ◽  
Soybean Plants ◽  
Soybean Leaves

Abstract Background Cry8-like from Bacillus thuringiensis (Bt) encodes an insecticidal crystal (Cry) protein. Holotrichia parallela (Coleoptera: Scarabaeoidae), commonly known as the dark black chafer, is a troublesome pest of soybean (Glycine max). To test whether cry8-like can confer resistance against H. parallela to soybean, we introduced cry8-like from the Bt strain HBF-18 into soybean cultivar Jinong 28. Results Quantitative reverse transcription-PCR analysis demonstrated that cry8-like was expressed most highly in soybean leaves. In addition, Southern blot assays revealed that one copy of the integrated fragment was present in the transformed plants. Eight independent cry8-like transgenic lines were subsequently fed on by H. parallela. Under H. parallela feeding stress, the survival rates of the non-transgenic plants were 92% lower than those of the transgenic plants. The mortality rate of H. parallela increased when the larvae fed on the roots of T1 transgenic soybean plants. Moreover, the surviving larvae were deformed, and their growth was inhibited. Conclusions Collectively, our data suggest that transgenic soybean plants expressing the cry8-like gene are more resistant to H. parallela than non-transgenic plants and that transgenic expression of the cry8-like gene may represent a promising strategy for engineering pest tolerance. The events generated in this study could thus be utilized in soybean breeding programs.

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