scholarly journals Cytogenetic and molecular studies in conservation breeding of Pulawska breed pigs

2017 ◽  
Vol 73 (7) ◽  
pp. 395-398 ◽  
Author(s):  
Marek Babicz ◽  
Barbara Danielak-Czech ◽  
Anna Kozubska-Sobocińska ◽  
Iwona Łuszczewska-Sierakowska ◽  
Agata Wawrzyniak ◽  
...  
Keyword(s):  
Dna Markers ◽  
Centromeric Heterochromatin ◽  
Production Traits ◽  
Specific Chromosome ◽  
Genetic Characteristics ◽  
Chromosome Markers ◽  
Nucleolar Organizing Regions ◽  
Molecular Studies ◽  
Polymorphic Variants ◽  
Conservation Breeding

The paper presents the genetic characteristics of Pulawska breed pigs carried out on the basis of cytogenetic and molecular studies. Among chromosome markers, polymorphic variants of specific chromosome structures such as centromeric heterochromatin areas (C bands) and nucleolar organizing regions (NOR bands) were taken into account. The described breed-specific tendencies concerning size polymorphism of these structures are the source of chromosome markers that are useful for identifying the linkage with genes controlling important production traits. On the other hand, molecular studies presented in this work included DNA markers of the STR and SNP type (with particular consideration of the PRL, FST, MC4R, TNNT3, MTTP and DIO3 genes), which are useful in determining the genetic background of functional traits, as well as the characteristics of the breeds for terms of genetic variation, especially conservative breeds, where it is appropriate to maintain the existing genetic diversity and intra-breed variability.

Specific chromosome markers involved with chronic T lymphocyte tumors

1986 ◽  
Vol 21 (1) ◽  
pp. 67-77 ◽  
Author(s):  
L. Zech ◽  
T. Godal ◽  
L. Hammarström ◽  
H. Mellstedt ◽  
C.I.E. Smith ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Specific Chromosome ◽  
Chromosome Markers

scholarly journals Geographic Origin and Genetic Characteristics of Japanese Indigenous Chickens Inferred from Mitochondrial D-Loop Region and Microsatellite DNA Markers

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2074
Author(s):  
Ayano Hata ◽  
Atsushi Takenouchi ◽  
Keiji Kinoshita ◽  
Momomi Hirokawa ◽  
Takeshi Igawa ◽  
...  
Keyword(s):  
Dna Markers ◽  
Microsatellite Dna ◽  
Genetic Relationships ◽  
Indigenous Chicken ◽  
Genetic Characteristics ◽  
Indigenous Chickens ◽  
Loop Region ◽  
Microsatellite Dna Markers ◽  
D Loop ◽  
Chicken Breeds

Japanese indigenous chickens have a long breeding history, possibly beginning 2000 years ago. Genetic characterization of Japanese indigenous chickens has been performed using mitochondrial D-loop region and microsatellite DNA markers. Their phylogenetic relationships with chickens worldwide and genetic variation within breeds have not yet been examined. In this study, the genetic characteristics of 38 Japanese indigenous chicken breeds were assessed by phylogenetic analyses of mitochondrial D-loop sequences compared with those of indigenous chicken breeds overseas. To evaluate the genetic relationships among Japanese indigenous chicken breeds, a STRUCTURE analysis was conducted using 27 microsatellite DNA markers. D-loop sequences of Japanese indigenous chickens were classified into five major haplogroups, A–E, among 15 haplogroups found in chickens worldwide. The haplogroup composition suggested that Japanese indigenous chickens originated mainly from China, with some originating from Southeast Asia. The STRUCTURE analyses revealed that Japanese indigenous chickens are genetically differentiated from chickens overseas; Japanese indigenous chicken breeds possess distinctive genetic characteristics, and Jidori breeds, which have been reared in various regions of Japan for a long time, are genetically close to each other. These results provide new insights into the history of chickens around Asia in addition to novel genetic data for the conservation of Japanese indigenous chickens.

scholarly journals SELECTION-GENETIC CHARACTERISTICS OF RABBITS POLTAVSKA SILVER BREED BY POLYMORPHISM OF PROGESTERONE RECEPTOR GENE

2020 ◽  
pp. 6-13
Author(s):  
Eugene Shevchenko ◽  
Oleksiy Honchar
Keyword(s):  
Progesterone Receptor ◽  
Receptor Gene ◽  
Multiple Pregnancy ◽  
Fixation Index ◽  
Pcr Analysis ◽  
Actual Distribution ◽  
Genetic Characteristics ◽  
Progesterone Receptor Gene ◽  
Significant Difference ◽  
Polymorphic Variants

Genetic certification of the Poltavska silver rabbits by the progesterone receptor gene was carried out by amplification of the corresponding sections of genomic DNA in the Polymerase Chain Reaction (PCR). As a result of PCR analysis it was found that the discrete DNA bands of rabbits were intensive hybridization spectra and characterized by different numbers, locations, and intensities of detected fragments. Based on the results of genotyping of Poltavska silver rabbits the following data on their genetic structure were obtained for gene polymorphism G2464A. In actual distribution of genotypes number of homozygous animals with the G gene was 18.3% (11 animals), number of homozygotes for A allele was almost twice as large (31.7%). The Wright fixation index for both polymorphic variants was negative which demonstrates the advantage of heterozygotes in population for tested genes. It was established that high values of multiple fertility rate in three aprons were found in rabbits with the GG genotype, and the lowest - with the AA genotype. In general there was a tendency to increase fertility by an average of 12% in three spouts for the “desired genotype” GG compared to animals that had genotypes AA and AG (p<0.05). Analysis of variation in milk yield of rabbits genotypes by polymorphic variants of progesterone receptor showed that this indicator was higher in homozygous animals AA, and the lowest - in heterozygotes AG. Moreover, rabbits with GG genotype were 3% inferior to them (the difference is not significant). According to the results of one-way analysis of variance, the influence of the rabbit genotype on the progesterone receptor gene on their milk production was found but no statistically significant difference between groups of animals with different genotypes. According to the data obtained it can be argued that the genotype of Poltavska Silver female rabbits in progesterone receptor gene has statistically significant effect on the manifestation of multiple pregnancy which can also be used in breeding.

scholarly journals CHROMOSOME MARKERS IN MUS MUSCULUS: STRAIN DIFFERENCES IN C-BANDING

Genetics ◽  
1973 ◽  
Vol 75 (4) ◽  
pp. 663-670
Author(s):  
V G Dev ◽  
D A Miller ◽  
O J Miller
Keyword(s):  
Mus Musculus ◽  
Strain Differences ◽  
Inbred Strains ◽  
F1 Hybrids ◽  
Centromeric Heterochromatin ◽  
Mitotic Chromosomes ◽  
Homologous Chromosomes ◽  
Chromosome Markers ◽  
C Banding ◽  
Inbred Strains Of Mice

ABSTRACT The mitotic chromosomes of several inbred strains of mice and a series of F1 hybrids have been analyzed by quinacrine staining and further characterized by the centromeric heterochromatin banding (C-banding). Inbred strains had the same amount of C-banding material on homologous chromosomes but showed variation in the amount on different chromosomes. F1 hybrids showed characteristics of each parent and it appears that the amount of C-banding on each chromosome is a simple inherited polymorphism. In this study 12 different chromosomes could be distinguished by their C-banding, and these can be used as normal chromosome markers.

scholarly journals Polymorphism of TGF-β1, TР53, CHEK2, ATM genes аnd chromosomal aberrations in workers at coal thermal electric power station

2021 ◽  
Vol 100 (1) ◽  
pp. 42-48
Author(s):  
Varvara I. Minina ◽  
Yulia A. Nelyubova ◽  
Anastaiya O. Sokolova ◽  
Yana A. Savchenko ◽  
Anastaiya V. Ryzhkova ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Power Plants ◽  
Thermal Power ◽  
Chromosomal Damage ◽  
Genetic Characteristics ◽  
Industrial Enterprises ◽  
Increased Risk ◽  
Thermal Electric Power Station ◽  
Polymorphic Variants ◽  
Tgf Β1

Introduction. Working conditions at coal-fired power plants cause an increased risk of the formation of chromosomal damage in workers. The contribution of the variability of the structure of the genes controlling the cell cycle and apoptosis to the occurrence of cytogenetic disorders induced by such mutagens has been little studied. Material and methods. The polymorphic variants of the genes: TP53 (rs1042522), CHEK2 (rs555607708), ATM (rs1801516), TGF-β1 (rs1800469), and the level of chromosomal aberrations (CA) in the lymphocytes of the blood were studied. Three hundred twenty-six workers of coal-fired power plants and 267 individuals who did not work in industrial enterprises were examined. All individuals were Caucasians, residents of Kemerovo (Western Siberia, Russia). Results. The level of CA in blood lymphocytes in thermal power plant workers was established to be statistically significantly higher than that of residents of the same locality who never works in industrial enterprises (3.05 ± 0.09% versus 1.67 ± 0.07%, p = 0, 000001). The prevalence of the polymorphic variants of the TP53 (rs1042522), CHEK2 (rs555607708), ATM (rs1801516), TGF-β1 (rs1800469) genes in the group of workers was the same to those in the comparison group and corresponded to the global data obtained in Caucasians. In the group of workers with the TT genotype of the TGF-β1 gene, the frequency of metaphases with chromosome damage was statistically significantly higher than in individuals with the CC genotype (4.07 ± 0.41% versus 2.85 ± 0.10%, p = 0.006). Discussion. For the first time a significant contribution of polymorphic variants of TGF-β1 genes to the formation of chromosomal damage in working coal-fired power plants was revealed. This may be associated with an increased level of expression of the multifunctional cytokine TGF-β1 in owners of the TT genotype and its ability to influence proliferation, apoptosis, telomerase activity, and other critical cellular processes. Conclusion. The results of the study I, ndicate that not only the complex of industrial genotoxicants, but also individual genetic characteristics of the organism can influence on the formation of cytogenetic disorders in workers at thermal power plants.

scholarly journals Molecular (ISSR, cp DNA, ITS) and Morphological Study of the Genus Tragopogon L. (Asteraceae)

2022 ◽  
Author(s):  
Hejraneh Azizi ◽  
Masoud Sheidai ◽  
Valiollah Mozaffarian ◽  
Zahra Noormohammadi
Keyword(s):  
Dna Markers ◽  
Morphological Study ◽  
Molecular Data ◽  
Interspecific Relationship ◽  
The World ◽  
Molecular Studies ◽  
Rapid Diversification ◽  
Species Hybridization

Abstract Tragopogon L. (Cichorioideae, Lactuceae, Scorzonerinae) is an Old World genus with 150 species, Rechinger in Flora Iranica divided this genus in 13 section and 37 species that 26 species of them are exist in Iran. Safavi et al. divided it into 26 species without sections in flora Iran. Despite the anatomical and molecular studies done around the world, the exact classification of this genus is not clear due to the high number of secret species, hybridization, polyploidy and rapid diversification. The morphology studies of 32 species and Molecular studies (ISSR, ITS, cp DNA) of 22 species of the genus Tragopogon was investigated . The purpose of these studies are classification and determination of interspecific relationship in this genus. Sections of Rubriflori, Sosnowskya, Chromopappus, Majores, Angustissimi, Krascheninnikovia in flora of Iranica are confirmed on the basis of morphometry and molecular data. Section of Profundisulcati in flora Iranica is confirmed on the base of morphometry data. The Species of T. jesdianus, T . porphyrocephalus, T. rezaiyensis and T. Stroterocarpus in the flora of Iranica are not classified in any section which we classified in the Rubriflori section, Cp DNA dendrogram are not useful for classification in this genus and Chloroplast sequences are very similar among Tragopogon species, Therefore, the use of cp DNA markers in the classification of this genus is not recommended.

scholarly journals Comparative Genetic Characteristics of Korean Ginseng using DNA Markers

2013 ◽  
Vol 21 (6) ◽  
pp. 444-454
Author(s):  
Mi Ran Shin ◽  
Ick Hyun Jo ◽  
Jong Wook Chung ◽  
Young Chang Kim ◽  
Seung Ho Lee ◽  
...  
Keyword(s):  
Dna Markers ◽  
Genetic Characteristics ◽  
Korean Ginseng

scholarly journals Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers

2018 ◽  
Vol 19 (4) ◽  
pp. 1593-1600
Author(s):  
SUTARNO . ◽  
NINA KURNIANINGRUM ◽  
ELISA HERAWATI ◽  
AHMAD DWI SETYAWAN
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
Dna Markers ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Polymorphic Information Content ◽  
Entire Genome ◽  
Genetic Characteristics ◽  
Microsatellite Dna Markers ◽  
The Mean

Sutarno, Kurnianingrum N, Herawati E, Setyawan AD. 2018. Interspecies and intraspecies genetic diversity of Ongole Gradecattle and Madura cattle based on microsatellite DNA markers. Biodiversitas 19: 1593-1600. DNA microsatellite has been extensivelyemployed for estimating the degree of kinship between genotypes and improving the quality of cattle products. Microsatellite markersare short-patterned DNA sequences and repeated tandem (sequentially) with 2-5 nucleotide units scattering the entire genome. Thepurpose of this study was to investigate the genetic characteristics of inter and intraspecies of Ongole Grade cattle and Madura cattleusing microsatellite DNA markers. Blood samples from 20 individuals of each species were extracted by the method referring to WizardGenomic DNA Purification Kit (Promega, USA) and PCR amplification was performed using 5 microsatellite loci, i.e., BM1824,ETH225, INRA005, MM12, and TGLA227. Results of the genetic characteristics of both species were calculated using the POPGENEprogram version 1.31. The data suggest that there is a genetic diversity of inter and intraspecies of Ongole Grade cattle and Maduracattle. The average value of Shannon's Information Index (I) at all microsatellite loci for Ongole Grade cattle was 0.76 and for Maduracattle was 1.12. Meanwhile, the average interspecies I value was 1.03. The mean intraspecies Polymorphic Information Content (PIC) ofOngole Grade and Madura cattle was 0.43, and 0.63, respectively, and the mean interspecies PIC value was 0.57. The data altogethersuggest that all loci meet the standards as being informative markers in the assessment of genetic population because it has a PIC value>0.5 especially for intraspecies of Madura cattle.

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