scholarly journals Effect of ultrasound waves on hematological parameters, behavior and conduction system of heart in vitro in BALB/c strain mouse

2019 ◽  
Vol 26 ◽  
pp. 74-77
Author(s):  
Algimantas Bubulis ◽  
Joris Vėžys ◽  
Judita Žymantienė ◽  
Rasa Želvytė ◽  
Vaidas Oberauskas ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Conduction System ◽  
Ultrasound Waves ◽  
Strain Mouse

Evaluation of the In Vivo Acute Toxicity and In Vitro Hemolytic and Immunomodulatory Activities of the Moringa oleifera Flower Trypsin Inhibitor (MoFTI)

2020 ◽  
Vol 27 ◽  
Author(s):  
Leydianne Leite de Siqueira Patriota ◽  
Dayane Kelly Dias do Nascimento Santos ◽  
Bárbara Rafaela da Silva Barros ◽  
Lethícia Maria de Souza Aguiar ◽  
Yasmym Araújo Silva ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Trypsin Inhibitor ◽  
Hemolytic Activity ◽  
Moringa Oleifera ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Behavioral Alterations ◽  
Female Mice

Background: Protease inhibitors have been isolated from plants and present several biological activities, including immunomod-ulatory action. Objective: This work aimed to evaluate a Moringa oleifera flower trypsin inhibitor (MoFTI) for acute toxicity in mice, hemolytic activity on mice erythrocytes and immunomodulatory effects on mice splenocytes. Methods: The acute toxicity was evaluated using Swiss female mice that received a single dose of the vehicle control or MoFTI (300 mg/kg, i.p.). Behavioral alterations were observed 15–240 min after administration, and survival, weight gain, and water and food consumption were analyzed daily. Organ weights and hematological parameters were analyzed after 14 days. Hemolytic activity of MoFTI was tested using Swiss female mice erythrocytes. Splenocytes obtained from BALB/c mice were cultured in the absence or presence of MoFTI for the evaluation of cell viability and proliferation. Mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) levels were also determined. Furthermore, the culture supernatants were analyzed for the presence of cytokines and nitric oxide (NO). Results: MoFTI did not cause death or any adverse effects on the mice except for abdominal contortions at 15–30 min after administration. MoFTI did not exhibit a significant hemolytic effect. In addition, MoFTI did not induce apoptosis or necrosis in splenocytes and had no effect on cell proliferation. Increases in cytosolic and mitochondrial ROS release, as well as ΔΨm reduction, were observed in MoFTI-treated cells. MoFTI was observed to induce TNF-α, IFN-γ, IL-6, IL-10, and NO release. Conclusion: These results contribute to the ongoing evaluation of the antitumor potential of MoFTI and its effects on other immunological targets.

scholarly journals Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 532
Author(s):  
Hae-Soo Yun ◽  
Sylvatrie-Danne Dinzouna-Boutamba ◽  
Sanghyun Lee ◽  
Zin Moon ◽  
Dongmi Kwak ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Hematological Parameters ◽  
Significant Inhibition ◽  
Ic50 Values ◽  
The Republic

In traditional Chinese medicine, Ranunculus japonicus has been used to treat various diseases, including malaria, and the young stem of R. japonicus is consumed as a food in the Republic of Korea. However, experimental evidence of the antimalarial effect of R. japonicus has not been evaluated. Therefore, the antimalarial activity of the extract of the young stem of R. japonicus was evaluated in vitro using both chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains; in vivo activity was evaluated in Plasmodium berghei-infected mice via oral administration followed by a four-day suppressive test focused on biochemical and hematological parameters. Exposure to extracts of R. japonicus resulted in significant inhibition of both chloroquine-sensitive (3D7) and resistant (Dd2) strains of P. falciparum, with IC50 values of 6.29 ± 2.78 and 5.36 ± 4.93 μg/mL, respectively. Administration of R. japonicus also resulted in potent antimalarial activity against P. berghei in infected mice with no associated toxicity; treatment also resulted in improved hepatic, renal, and hematologic parameters. These results demonstrate the antimalarial effects of R. japonicus both in vitro and in vivo with no apparent toxicity.

scholarly journals Novel scaffold based graphene oxide doped electrospun iota carrageenan/polyvinyl alcohol for wound healing and pathogen reduction: in-vitro and in-vivo study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marwa H. Gouda ◽  
Safaa M. Ali ◽  
Sarah Samir Othman ◽  
Samia A. Abd Al-Aziz ◽  
Marwa M. Abu-Serie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Hematological Parameters ◽  
Nutritional Supplement ◽  
Hair Follicles ◽  
Nanofiber Scaffold ◽  
Novel Scaffold

AbstractWound healing is a complicated multicellular process that involves several kinds of cells including macrophages, fibroblasts, endothelial cells, keratinocytes and platelets that are leading to their differentiation towards an anti-inflammatory response for producing several chemokines, cytokine and growth factors. In this study, electrospun nanofiber scaffold named (MNS) is composed of polyvinyl alcohol (PVA)/iota carrageenan (IC) and doped with partially reduced graphene oxide (prGO) that is successfully synthesized for wound healing and skin repair. The fabricated MNS was tested in case of infection and un-infection with E. coli and Staphylococcus and in both of the presence and in the absence of yeast as a natural nutritional supplement. Numerous biochemical parameters including total protein, albumin, urea and LDH, and hematological parameters were evaluated. Results revealed that the MNS was proved to be effective on most of the measured parameters and had exhibited efficient antibacterial inhibition activity. Whereas it can be used as an effective antimicrobial agent in wound healing, however, histopathological findings confirmed that the MNS caused re-epithelialization and the presence of yeast induced hair follicles growth and subsequently it may be used to hide formed head wound scar.

scholarly journals Determination of in Vivo Biological Activities of Dodonaea Viscosa Flowers Against CCL4 Toxicity in Albino Mice With Bioactive Compound Detection

2021 ◽  
Author(s):  
Hina Gul ◽  
Muhammad Awais ◽  
Salina Saddick ◽  
Falak Sher Khan ◽  
Muhammad Gulfraz ◽  
...  
Keyword(s):  
Protective Effect ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Octadecenoic Acid ◽  
Radical Scavenging ◽  
Herbal Medicines ◽  
Bioactive Compound ◽  
Dodonaea Viscosa

Abstract Dodonaea viscosa L. Jacq. is an evergreen shrub and native to Asia, Africa and Australia. It has been used as traditional medicine in different countries. The foremost objective of the current study was to discover protective potential of D. Viscosa flowers Methanol (DVM) and Chloroform (DVC) extracts against CCL4 induced toxicity in mice. This study was intended to identify phytochemicals through HPLC, GCMS and FT-IR as well as in vitro antioxidant and in vitro antituberculosis activity. Our comprehensive findings indicate that Dodonaea viscosa is valuable and widespread herbal medicines through therapeutic potentials for curing various ailments. Dodonaea viscosa flowers are found to have protective effect against oxidative stress produced by CCL4 in liver, kidney and spleen. The level of hepatic enzymes (ALP, AST ALT and Direct bilirubin), hematological parameters (RBCs, WBCs and Platelets), total protein and liver antioxidant enzymes (SOD, GPx and CAT) were restored by the intake of DV extracts after decline in levels by CCL4. Histopathological results discovered the defensive effect of 300mg/kg of DVM extract against CCL4 induced damage, thus having improved protective effect as compared to DVC and control. As a result of analysis total flavonoids and total phenolics were also revealed. Phytochemical investigation by HPLC identified gallic acid, epicatechin, cumeric acid, flavonoids while Oleic acid (Octadecenoic acid) (C18H34O2), Stearic acid (C18H36O2), Ricinoleic acid (C18H34O3) and Cedrol (C15H26O) was estimated by GCMS. DVM extract exhibited resistance against in vitro Mycobacterium tuberculosis strains. This study proposed that protective effect of DV against oxidative damage induced in Liver, Kidney and Spleen can possibly be correlated to their antioxidant as well as free radical scavenging property.

scholarly journals In vitro and in vivo Activities of Psidium guajava and Azadirachta indica Leaf Extracts and solvent Fractions against Salmonella Typhi

2021 ◽  
pp. 56-71
Author(s):  
Adetutu Adewale ◽  
Olaniyi Deborah Temitope ◽  
Awodugba Tamilore ◽  
Owoade Abiodun Olusoji ◽  
Olaniyan, Lamidi Waheed B. ◽  
...  
Keyword(s):  
Body Weight ◽  
Hematological Parameters ◽  
Sub Saharan Africa ◽  
Male Rats ◽  
Carrier Status ◽  
Leaf Extracts ◽  
Salmonella Infections ◽  
Neem Leaves

Typhoidal salmonella infections remain a challenge in the health care system in sub-Saharan Africa. Carrier status and advent of multi-drug resistant S. Typhi strains have necessitated the search for new drug leads. Hence, this study aims at investigating P. guajava and A. indica leaves for anti-salmonella activities. Guava and neem leaves were extracted by maceration in methanol and fractionated by solvent partitioning. In vitro activities were assessed by agar well diffusion and broth micro-dilution methods. Sixty male rats were randomized to 10 groups of 6 animals each for the in vivo experiments. Groups of rats except, normal control, were induced with 0.5McFarland of S. Typhi suspension orally. Treatment groups received 200 mg/kg body weight of extracts and fractions, and the control groups were treated with 14.29mg/kg body weight of ciprofloxacin and 1%v/v DMSO for 7 days post-infection. Biochemical parameters were determined spectrophotometrically. Hematological parameters were analyzed with automated hematology diagnostic machine. All fractions of P. guajava and three of A. indica inhibited S. Typhi growth with Zone of Inhibition (ZI) ranging from 11-15 mm. Active fractions inhibited 48.60-62.45% of S. Typhi biofilm formation at 25 mg/mL with Minimum Bactericidal Inhibitory Concentration (MBIC) of 0.39-12.5 mg/mL. All fractions improved body weight of treated rats and inhibited bacteremia at 44.75 and 95.94%. Hematological parameters improved in all fractions-treated rats. MDA was not significantly (p<0.05) altered in all groups. One fraction of P. guajava (ePg) lowered the elevated level in concentration of Nitric oxide (NO) while all fractions enhanced the lowered activity of SOD. Elevated (lactate dehydrogenase (LDH), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and bilirubin (BIL) were lowered by all fractions to various extents in treated rats. Fractions of P. guajava, and A. indica could be further considered for identification of active anti-salmonella principle(s).

scholarly journals Evaluation of the hemogram of Oryctolagus cuniculus envenomus

2020 ◽  
Vol 13 (2) ◽  
pp. 266-272
Author(s):  
Obou Constantin Okou ◽  
N’guessan Emmanuel Assemian ◽  
Kouadio Bernard Allali ◽  
Guy Childeric Bingo ◽  
Allico Joseph Djaman
Keyword(s):  
Blood Cells ◽  
Oryctolagus Cuniculus ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Experimental Batch ◽  
Haematological Parameters ◽  
Rabbit Blood ◽  
Hemolysis Test ◽  
Dose Dependent

The overall objective of this study was to evaluate the hemolysing action of Naja nigricollis venom on rabbit blood. To carry out this study, three batches of three rabbits were formed with two control batches and one experimental batch. Each control lot is composed of three rabbits (males or females) while the experimental lot is composed of two males and one female. Each rabbit from the control lots was separately collected in the purple tube (EDTA) and transported to the laboratory for analysis. The rabbits from the experimental batch were also collected distinctly a few minutes after the injection of the venom of Naja nigricollis for the analysis of haematological parameters. However, before the analysis of the hematological parameters of the rabbits from the control and experimental batches, an in vitro hemolysis test of Naja nigricollis venom was performed to verify its hemolysing power. The results showed that Naja nigricollis venom has a dose-dependent in vitro hemolysing power. As for the haemogram, it revealed that the venom of Naja nigricollis has a decreasing effect on blood cells (red and white blood cells), on haemoglobin and on haematocrit, and an elevation on MGVs thus promoting anaemia.

In vitro anticancer activity of Astaxanthin

2021 ◽  
Vol 5 (3) ◽  
pp. 033-037
Author(s):  
Uma Nath U ◽  
Ravi. R ◽  
Sundara Ganapathy ◽  
Lal Prasanth
Keyword(s):  
Anticancer Activity ◽  
Tumor Volume ◽  
Hematological Parameters ◽  
Ehrlich Ascites Carcinoma ◽  
High Dose ◽  
Ehrlich Ascites ◽  
Shrimp Shell ◽  
Shrimp Shell Waste ◽  
Wbc Count

This study was designed to determine the in vitro anticancer potential of the Astaxanthin isolated from shrimp shell waste (ETC) against Ehrlich Ascites Carcinoma (EAC) induced cancer in swiss albino mice. The anticancer activity was assessed using in vitro cytotoxicAity, mean survival time, tumor volume and hematological studies. The reliable criteria for evaluating the potential of any anticancer agent is the prolongation of lifespan of the animal and decrease in WBC count of blood. The high dose of ETC (200 mg/kg, orally) significantly reduced the tumor growth which was demonstrated by increased lifespan of the mice and restoration of hematological parameters. ETC was also found to be cytotoxic in the in vitro parameter which shows that ETC possesses significant anticancer potential.

Anti-inflammatory, Antioxidant, Lung and Liver Protective Activity of Galaxaura oblongata as Antagonistic Efficacy against LPS using Hematological Parameters and Immunohistochemistry as Biomarkers

2021 ◽  
Vol 19 ◽  
Author(s):  
Asmaa Nabil-Adam ◽  
Mohamed A. Shreadah
Keyword(s):  
Protective Effect ◽  
Hematological Parameters ◽  
Control Group ◽  
In Vivo Assays ◽  
Induction Group ◽  
Anti Inflammatory ◽  
In Vitro Effects

Background: This study aimed to investigate the potential bioactivity and the ameliorative role of Galaxaura oblongata (G. oblongata) against LPS-induced toxicity by using hematological parameters. Objective: It is aimed also to examine its protective effect using the immunohistochemistry of liver and lungs as biomarkers in male BALB/C albino mice. Materials and Methods: the current study carried out using different in-vitro and in-vivo assays such as phytochemical, antioxidants, anti-inflammatory for in-vitro where the hematological and immunohistochemistry for lung and liver were investigated in vivo. Results: There are no previous studies were performed to investigate the in vivo and in vitro effects of the G. oblongata extracts as antioxidant and anti-inflammatory due to their rareness compared to other red algae. LPS treated mice revealed a significant decrease in total number of WBCs, RBCs, platelets, and HGB%, MPV, MCV and MCHC compared to the control group. On contrast, the HCT and MCHC were increased in the induction group which was treated with LPS compared to the control group. Furthermore, the immunohistochemistry results of the present study revealed the protective effect of G. oblongata compared to the induction group. G. oblongata can be used as protective marine natural products against the toxicity induced by LPS. Conclusion: It exhibited a significant ameliorative role against the alterations in the hematological parameters and immunohistochemistry of liver and lungs, and helps to reduce as well as coordinate the acute inflammations caused by TNF.

The Role of Gold Nanoparticles in Sonosensitization of Human Cervical Carcinoma Cell Line under Ultrasound Irradiation: An In Vitro Study

2019 ◽  
Vol 59 ◽  
pp. 1-14 ◽  
Author(s):  
Ahmad Shanei ◽  
Hadi Akbari-Zadeh ◽  
Hamid Fakhimikabir ◽  
Neda Attaran
Keyword(s):  
Gold Nanoparticles ◽  
Hela Cells ◽  
Ultrasound Irradiation ◽  
Ultrasound Wave ◽  
Efficient Treatment ◽  
Ultrasound Waves ◽  
Cervical Carcinoma Cell Line ◽  
Ultrasound Intensity ◽  
High Concentrations

Abstract:Purpose: The objective of this investigation was to evaluate the combined effects of ultrasound irradiation as a non-invasive and non-ionizing radiation with gold nanoparticles as ultrasound sensitizers on the HeLa cells.Materials and Methods: First, Gold nanoparticles (GNPs) were prepared, and the characterizations of nanoparticles were analyzed using TEM and UV-vis. Different concentrations of nanoparticles (0.2, 1, 5, 25 and 50 μg/ml) were used. Then, cytotoxicity of the GNPs was studied on HeLa cells, and finally concentrations of 0.2, 1 and 5 µg/mL were chosen for supplementary studies. The effects of nanoparticles and ultrasound irradiation with different intensities (0.5, 1 and 1.5 W/cm2) as well as the combination of ultrasound radiation with various concentrations of nanoparticles on 24, 48 and 72 h post-experiment cells’ viability were estimated by MTT and trypan blue assay.Results: Results showed that the sonosensitizing effect of nanoparticles mainly depended on the intensity of ultrasound waves and the concentration of GNPs. By increasing the concentration of GNPs and ultrasound intensity, the nanoparticle's effect of sensitizer was also increased. Moreover, as expected, the highest effect was observed at the highest intensities (1.5 W/cm2) of ultrasound wave and high concentrations (5 μg/ml) of GNPs 72 h after exposure to ultrasound irradiation.Conclusions: It is revealed that GNPs can be used as sonosensitizers of HeLa cells to ultrasound irradiation in order to produce an efficient treatment.

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