PROTECTIVE EFFECT OF GRAPE SEEDS EXTRACT AGAINST SODIUM NITRITE-INDUCED TOXICITY AND OXIDATIVE STRESS IN ALBINO RATS

The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant-antioxidant balance and oxidative stress on certain tissues and the possible protective effect of safranal against thinner toxication in rats. Adult male Wistar albino rats were randomly divided into four groups of 10 animals each as follows: control (C), safranal (S), thinner (T) and thinner+safranal (T+S). The control group received 1cc saline by gastric gavage. Safranal was administered to S and T+S groups by using gastric gavage at a dose of 100 mg/kg/day and volume of 0.1 mL/kg/day. Thinner inhalation was applied to T and T+S groups in a container with NaOH tablets twice a day. Levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NOx) metabolites, total antioxidant capacity (TAS) and total oxidant capacity (TOS) were determined in liver, lung, brain, kidney and testis tissues of the rats. In the T+S group, it was observed that the MDA levels significantly decreased in all tissues, except the kidney, in comparison to the thinner inhalation group (p = 0.000). When the NOx levels of the T+S group were compared with the levels of the T group, it was concluded that there existed a statistically significant decrease in the NOx levels in alltissues (p = 0.000). In T+S group, it was observed that safranal either eliminated or mitigated oxidative stress that developed in tissues through decreasing MDA and TOS levels and increasing GSH and TAS levels and caused significant decreases in NOX levels in all tissues. As a result, it was determined that safranal, although not uniform for all tissue types, had a protective potential against the damaging effects of oxidative stress caused by sub-chronic thinner inhalation.

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Evaluation of the protective effect of coded plant leaf ethanolic extract (222) against paracetamol-induced hepatotoxicity and oxidative stress in Wistar albino rats - Part III

Journal of Traditional and Folk Practices ◽

10.25173/jtfp.2017.5.1.60 ◽

2017 ◽

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Ethanolic Extract ◽

Albino Rats ◽

Plant Leaf ◽

Wistar Albino Rats ◽

And Oxidative Stress

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The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats

Biochemistry Research International ◽

10.1155/2017/9478958 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 18

Author(s):

Ismail Koyuncu ◽

Abdurrahim Kocyigit ◽

Ataman Gonel ◽

Erkan Arslan ◽

Mustafa Durgun

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Comet Assay ◽

Protective Effect ◽

Stress Index ◽

Mononuclear Leukocytes ◽

Albino Rats ◽

Peripheral Blood Mononuclear ◽

Liver And Kidney ◽

And Oxidative Stress

The aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/kg b.w.), NOX treatment (i.p., 20 mg/kg b.w., daily for ten days), Cis + NOX20, and Cis + NOX40 combination (i.p., 20 and 40 mg/kg b.w., daily for ten days). Serum and peripheral blood mononuclear leukocytes (PBMC) were obtained from blood. Malondialdehyde, glutathione, total antioxidant and oxidant status, and catalase were measured in serum, liver, and kidney, and oxidative stress index was calculated. In parallel, paraoxonase and arylesterase activities were tested in liver and serum. We used 8-OHdOG as a marker for DNA damage in serum via ELISA and in PMBC via comet assay. Treatment with Cis elevated the levels of serum biochemical parameters, oxidative stress, and DNA damage. Pretreatments of NOX restored biochemical and oxidative stress parameters in serum, renal, and liver tissues (p<0.01) and reduced 8-OHdG level, a finding further supported by comet assay in PBMC. Observations of the present study support the fact that treatment with NOX prevents Cis-induced hepatotoxicity, nephrotoxicity, and genotoxicity by restoring antioxidant system.

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Protective effect of nanoencapsulated curcumin against boldenone-induced testicular toxicity and oxidative stress in male albino rats

Egyptian Pharmaceutical Journal ◽

10.4103/epj.epj_53_20 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0

Author(s):

Marwa El-Sayed El-Shamarka ◽

MohamedA.S Aly ◽

TarekN Soliman ◽

MahmoudA.E Elgabry

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Albino Rats ◽

Testicular Toxicity ◽

And Oxidative Stress

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Antia, a Natural Antioxidant Product, Attenuates Cognitive Dysfunction in Streptozotocin-Induced Mouse Model of Sporadic Alzheimer’s Disease by Targeting the Amyloidogenic, Inflammatory, Autophagy, and Oxidative Stress Pathways

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/4386562 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Nesrine S. El Sayed ◽

Mamdooh H. Ghoneum

Keyword(s):

Oxidative Stress ◽

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Neurodegenerative Diseases ◽

Protective Effect ◽

Cognitive Dysfunction ◽

Protective Role ◽

Sporadic Alzheimer’S Disease ◽

Stat3 Pathway ◽

And Oxidative Stress

Background. Many neurodegenerative diseases such as Alzheimer’s disease are associated with oxidative stress. Therefore, antioxidant therapy has been suggested for the prevention and treatment of neurodegenerative diseases. Objective. We investigated the ability of the antioxidant Antia to exert a protective effect against sporadic Alzheimer’s disease (SAD) induced in mice. Antia is a natural product that is extracted from the edible yamabushitake mushroom, the gotsukora and kothala himbutu plants, diosgenin (an extract from wild yam tubers), and amla (Indian gooseberry) after treatment with MRN-100. Methods. Single intracerebroventricular (ICV) injection of streptozotocin (STZ) (3 mg/kg) was used for induction of SAD in mice. Antia was injected intraperitoneally (i.p.) in 3 doses (25, 50, and 100 mg/kg/day) for 21 days. Neurobehavioral tests were conducted within 24 h after the last day of injection. Afterwards, mice were sacrificed and their hippocampi were rapidly excised, weighed, and homogenized to be used for measuring biochemical parameters. Results. Treatment with Antia significantly improved mice performance in the Morris water maze. In addition, biochemical analysis showed that Antia exerted a protective effect for several compounds, including GSH, MDA, NF-κB, IL-6, TNF-α, and amyloid β. Further studies with western blot showed the protective effect of Antia for the JAK2/STAT3 pathway. Conclusions. Antia exerts a significant protection against cognitive dysfunction induced by ICV-STZ injection. This effect is achieved through targeting of the amyloidogenic, inflammatory, and oxidative stress pathways. The JAK2/STAT3 pathway plays a protective role for neuroinflammatory and neurodegenerative diseases such as SAD.

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Effect of selenium on carbimazole-induced testicular damage and oxidative stress in albino rats

Journal of Trace Elements in Medicine and Biology ◽

10.1016/j.jtemb.2010.07.002 ◽

2011 ◽

Vol 25 (1) ◽

pp. 59-66 ◽

Cited By ~ 24

Author(s):

Saber Abdul Ruhman Sakr ◽

Hoda Abdel-hafez Mahran ◽

Amany Ebrahem Nofal

Keyword(s):

Oxidative Stress ◽

Albino Rats ◽

Testicular Damage ◽

And Oxidative Stress

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Changes of platelet antioxidative enzymes during oxidative stress: The protective effect of polyphenol-rich extract from berries ofAronia melanocarpaand grape seeds

Platelets ◽

10.3109/09537104.2010.545151 ◽

2011 ◽

Vol 22 (5) ◽

pp. 385-389 ◽

Cited By ~ 10

Author(s):

Magdalena Kedzierska ◽

Beata Olas ◽

Barbara Wachowicz ◽

Anna Stochmal ◽

Wiesław Oleszek ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Antioxidative Enzymes ◽

Grape Seeds

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Reduction of doxorubicin-induced cytotoxicity and mitochondrial damage by betanin in rat isolated cardiomyocytes and mitochondria

Human & Experimental Toxicology ◽

10.1177/09603271211022800 ◽

2021 ◽

pp. 096032712110228

Author(s):

AA Hafez ◽

Z Jamali ◽

S Samiei ◽

S Khezri ◽

A Salimi

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Bioactive Compound ◽

Isolated Cardiomyocytes ◽

Cardiac Inflammation ◽

Flow Cytometric ◽

Significant Toxicity ◽

Ros Formation ◽

Gene Regulatory ◽

And Oxidative Stress

Doxorubicin (DOX) is an anticancer drug which is used for treatment of several types of cancers. But the clinical use of doxorubicin is limited because of its cardiotoxicity and cardiomyopathy. Mitochondrial-dependent oxidative stress and cardiac inflammation appear to be involved in doxorubicin-induced cardiotoxicity. Betanin as a bioactive compound in Beetroot ( Beta vulgaris L.) displays anti-radical, antioxidant gene regulatory and cardioprotective activities. In this current study, we investigated the protective effect of betanin on doxorubicin-induced cytotoxicity and mitochondrial-dependent oxidative stress in isolated cardiomyocytes and mitochondria. Isolated cardiomyocytes and mitochondria were treated with three concentrations of betanin (1, 5 and 10 µM) and doxorubicin (3.5 µM) for 6 h. The parameters of cellular and mitochondrial toxicity were analyzed using biochemical and flow cytometric methods. Our results showed a significant toxicity in isolated cardiomyocytes and mitochondria in presence of doxorubicin which was related to reactive oxygen species (ROS) formation, increase in malondialdehyde (MDA), increase in oxidation of GSH to GSSG, lysosomal/mitochondrial damages and mitochondrial swelling. While betanin pretreatment reverted doxorubicin-induced cytotoxicity and oxidative stress in isolated cardiomyocytes and mitochondria. These results suggest that betanin elicited a typical protective effect on doxorubicin-induced cytotoxicity and oxidative stress. It is possible that betanin could be used as a useful adjuvant in combination with doxorubicin chemotherapy for reduction of cardiotoxicity and cardiomyopathy.

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