Mitigation of Genotoxic and Cytotoxic Effects of Silver Nanoparticles on Onion Root Tips using some Antioxidant Scavengers

2019 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Rehab Hafez ◽  
Ahmed Fouad
Keyword(s):  
Silver Nanoparticles ◽  
Root Tips ◽  
Cytotoxic Effects ◽  
Onion Root

Antibacterial and Cytotoxic Effects of Silver Nanoparticles on Staphylococcus aureus and Normal Vero Cells

2019 ◽  
Vol 22 (10) ◽  
pp. 184-190
Author(s):  
Rasha Hadi Saleh ◽  
Entisar J. Al-Mukhtar ◽  
Zaytoon A. Al-Khafaji ◽  
Mohammed H. Al Hasnawy ◽  
Huda H. Al-Hasnawy
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Vero Cells ◽  
Cytotoxic Effects

scholarly journals Silver Nanoparticles and Their Antibacterial Applications

2021 ◽  
Vol 22 (13) ◽  
pp. 7202
Author(s):  
Tamara Bruna ◽  
Francisca Maldonado-Bravo ◽  
Paul Jara ◽  
Nelson Caro
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Agents ◽  
Multidrug Resistant ◽  
Secondary Effects ◽  
Cytotoxic Effects ◽  
Factors Affecting ◽  
Gram Positive Bacteria ◽  
Resistant Strains

Silver nanoparticles (AgNPs) have been imposed as an excellent antimicrobial agent being able to combat bacteria in vitro and in vivo causing infections. The antibacterial capacity of AgNPs covers Gram-negative and Gram-positive bacteria, including multidrug resistant strains. AgNPs exhibit multiple and simultaneous mechanisms of action and in combination with antibacterial agents as organic compounds or antibiotics it has shown synergistic effect against pathogens bacteria such as Escherichia coli and Staphylococcus aureus. The characteristics of silver nanoparticles make them suitable for their application in medical and healthcare products where they may treat infections or prevent them efficiently. With the urgent need for new efficient antibacterial agents, this review aims to establish factors affecting antibacterial and cytotoxic effects of silver nanoparticles, as well as to expose the advantages of using AgNPs as new antibacterial agents in combination with antibiotic, which will reduce the dosage needed and prevent secondary effects associated to both.

Glycosyltransferases in plant and animal tissues effects of fixation and lead on enzyme activity.

1978 ◽  
Vol 26 (10) ◽  
pp. 772-781 ◽  
Author(s):  
W D Klohs ◽  
C W Goff ◽  
R J Bernacki
Keyword(s):  
Enzyme Activity ◽  
Lead Nitrate ◽  
Initial Step ◽  
Lead Ions ◽  
Fixation Time ◽  
Animal Tissues ◽  
Root Tips ◽  
Cytochemical Localization ◽  
Onion Root ◽  
L1210 Cells

As the initial step toward the cytochemical localization of glycosyl-transferases in situ, biochemical determinations of these enzyme activities from onion root tips and L1210 cells were performed before and after fixation as well as in the presence of lead ions. Glycosyltransferase activity from roots fixed in buffered formaldehyde or glutaraldehyde before homogenization decreased as the concentration of the fixative or fixation time was increased. Formaldehyde fixation was less inhibitory than glutaraldehyde; 35% of the glycosyltransferase activity was retained after 30 min fixation in 2% formaldehyde while 25% of the enzyme activity remained after a similar fixation in glutaraldehyde. Substantially higher levels of L1210 cell glycosyltransferase activity were retained after a 30 min 2% formaldehyde fixation (60% sialyltransferase; 82% galactosyltransferase), but inhibition by glutaraldehyde was similar to that observed for onion root galactosyltransferase. Glycosyltransferase from formaldehyde-fixed roots was inhbited 35% by lead nitrate, but sialytransferase from formaldehyde-fixed L1210 cells was unaffected by lead ions. These findings are encouraging for further studies aimed at the development of cytochemical technique to localize glycosyltransferase in plant and animal tissues.

In Vitro Evaluation of Anti-Mitotic Activity of Green Synthesized Silver Nanoparticles from Ethanolic Extract of Chromolaena odorata, Caesalpinia coriaria (Bark and Leaves) using Allium cepa Roots

2021 ◽  
pp. 4107-4110
Author(s):  
Nishita Gogia ◽  
Anandhi D ◽  
Kanaga G ◽  
Revathi K
Keyword(s):  
Silver Nanoparticles ◽  
Mitotic Activity ◽  
Mitotic Index ◽  
Allium Cepa ◽  
Chromolaena Odorata ◽  
Root Tips ◽  
Antimitotic Activity ◽  
Biological Potential ◽  
Green Synthesized Silver Nanoparticles

The scope of the present study was to evaluate the In-vitro biological potential of Green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf). We investigated the effect of green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) by studying anti-mitotic activity An attempt has been made to evaluate the anti-mitotic activity of silver nanoparticles using Allium cepa roots. The mitotic index of the root tips meristem was calculated and compared with the standard methotrexate. Antimitotic activity results shows the mitotic index was ranged from 30-40%. Green synthesized silver nanoparticles from Chromolaena odorato, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) possess significant anti-mitotic activity.

Cellular Respiration in Onion Root Tips

1959 ◽  
Vol 120 (3) ◽  
pp. 131-137 ◽  
Author(s):  
W. E. Norris, ◽  
E. J. Harber ◽  
J. E. Butler
Keyword(s):  
Cellular Respiration ◽  
Root Tips ◽  
Onion Root

scholarly journals THE USE OF BISMUTH AS AN ELECTRON STAIN FOR NUCLEIC ACIDS

1963 ◽  
Vol 17 (1) ◽  
pp. 93-103 ◽  
Author(s):  
Peter Albersheim ◽  
Ursula Killias
Keyword(s):  
Nucleic Acids ◽  
Inorganic Phosphate ◽  
In Vitro Experiments ◽  
Root Tips ◽  
Onion Root ◽  
Cell Structures ◽  
Dividing Cells ◽  
Chromatin Material

Evidence is presented to show that bismuth combines in vitro with the phosphate of nucleic acids in a manner similar to its reaction with inorganic phosphate. When tested under similar conditions, protein exhibited no attraction for bismuth. The results of the in vitro experiments, which are of interest within themselves, may be indirectly applicable to in vivo staining. Dividing cells of onion root tips were fixed in OsO4, stained with bismuth, and examined in the electron microscope. The electron opacity of cell structures known to contain nucleic acids was enhanced by bismuth, while organelles known to lack appreciable quantities of DNA or RNA showed little, if any, change. Bismuth is particularly effective as a stain for the chromatin material during interphase and for the chromosomes during division.

Serum albumin reduces the antibacterial and cytotoxic effects of hydrogel-embedded colloidal silver nanoparticles

RSC Advances ◽  
2012 ◽  
Vol 2 (18) ◽  
pp. 7190 ◽  
Author(s):  
Sebastian Grade ◽  
Jörg Eberhard ◽  
Anne Neumeister ◽  
Philipp Wagener ◽  
Andreas Winkel ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Serum Albumin ◽  
Colloidal Silver ◽  
Cytotoxic Effects ◽  
Colloidal Silver Nanoparticles
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