Prevalence of Listeria Species and Serotyping of Listeria monocytogenes Bacteria Isolated from Seafood Samples

2021 ◽  
Vol 52 (1) ◽  
pp. 1-9
Author(s):  
Zohreh Mashak ◽  
Fatemeh Banisharif ◽  
Gholamreza Banisharif ◽  
Mostafa Reza Pourian ◽  
Soheyl Eskandari ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Listeria Species

Optical forward-scattering for detection of Listeria monocytogenes and other Listeria species

2007 ◽  
Vol 22 (8) ◽  
pp. 1664-1671 ◽  
Author(s):  
Padmapriya P. Banada ◽  
Songling Guo ◽  
Bulent Bayraktar ◽  
Euiwon Bae ◽  
Bartek Rajwa ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Forward Scattering ◽  
Listeria Species

Listeria monocytogenes in Raw Milk: Detection, Incidence, and Pathogenicity

1987 ◽  
Vol 50 (3) ◽  
pp. 188-192 ◽  
Author(s):  
J. LOVETT ◽  
D. W. FRANCIS ◽  
J. M. HUNT
Keyword(s):  
United States ◽  
Listeria Monocytogenes ◽  
Sampling Period ◽  
Raw Milk ◽  
The United States ◽  
Isolation Method ◽  
Listeria Species ◽  
Adult Mice ◽  
Listeria Ivanovii

To determine the incidence of Listeria monocytogenes in raw milk, an isolation method was evaluated and used to analyze milk from three areas of the United States. The incidence varied by area from 0% in California to 7% in Massachusetts, with an overall incidence of 4.2%. The highest incidence found in any area during a single sampling period was 12% in Massachusetts in March 1985. During that same sampling, the incidence for all Listeria species was 26%. Of the 27 L. monocytogenes strains isolated during the survey, 25 were pathogenic in adult mice. One of three Listeria ivanovii isolated was pathogenic. No other isolates demonstrated pathogenicity.

Occurrence of Listeria monocytogenes in Milk and Dairy Products Produced or Imported Into Morocco

1993 ◽  
Vol 56 (3) ◽  
pp. 256-259 ◽  
Author(s):  
A. EL MARRAKCHI ◽  
A. HAMAMA ◽  
F. EL OTHMANI
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Pasteurized Milk ◽  
Listeria Species ◽  
Fresh Cheese ◽  
Milk And Dairy Products

Examination of 227 samples of milk and dairy products for Listeria monocytogenes showed that raw milk and some Moroccan traditionally made dairy products such as Iben and raib (fermented milks) and jben (fresh cheese) were contaminated with this pathogen. L. monocytogenes was the only Listeria species isolated except in one case in which it was associated with Listeria innocua. Pasteurized milk, fresh cream, and fresh and ripened cheeses (industrially made) were free from L. monocytogenes.

scholarly journals Assurance Polyclonal Enzyme Immunoassay for Detection of Listeria monocytogenes and Related Listeria Species in Selected Foods: Collaborative Study

1997 ◽  
Vol 80 (4) ◽  
pp. 775-790 ◽  
Author(s):  
Philip T Feldsine ◽  
Andrew H Lienau ◽  
Robin L Forgey ◽  
Roger D Calhoon ◽  
S Al-Hasani ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Enzyme Immunoassay ◽  
Collaborative Study ◽  
Culture Method ◽  
The United States ◽  
Food Type ◽  
Green Beans ◽  
Private Industry ◽  
Listeria Species ◽  
Two Samples

Abstract Six foods representing a variety of food products were analyzed by the Assurance Listeria polyclonal enzyme immunoassay (EIA) and by either the Bacteriological Analytical Manual or the U.S. Department of Agriculture culture method for detecting Listeria monocytogenes and related Listeria species. Samples of each food type, at each inoculation level, were analyzed simultaneously by both methods. A total of 19 laboratories representing federal government agencies and private industry in the United States and Canada participated. Food types were inoculated with Listeria species including L. monocytogenes, with the exception of 3 lots of green beans, which were naturally contaminated. During this study, 1764 samples and controls were analyzed and confirmed, of which 492 were positive and 947 were negative by both methods. There were 159 samples that were positive by culture method but negative by the EIA and 188 that were negative by culture method but positive by EIA. Twenty-two samples were negative by EIA and by culture method but confirmed positive when Assurance selective enrichment broths were subcultured to selective agar. The Assurance polyclonal EIA for detecting L. monocytogenes and related Listeria species in foods has been adopted first action by AOAC INTERNATIONAL.

Incidence of Listeria monocytogenes in Silage and Its Subsequent Control by Specific and Nonspecific Antagonism

1990 ◽  
Vol 53 (8) ◽  
pp. 642-647 ◽  
Author(s):  
CURTT M. PERRY ◽  
CATHERINE W. DONNELLY
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Dairy Farms ◽  
Listeria Innocua ◽  
Listeria Species

Silage samples representing approximately 10% of Vermont's dairy farms were tested for the presence of Listeria species. Listeria innocua was isolated from 15.3% of the silage samples, while Listeria monocytogenes was isolated from 2.9% of the examined samples. As silage pH increased, the incidence of Listeria increased concomitantly. Seventy-eight mesophilic lactic acid bacteria, indigenous to silage, were screened for specific and nonspecific antagonism against four L. monocytogenes indicator strains. Most of the silage isolates demonstrated nonspecific inhibition via lactic acid production against the L. monocytogenes indicator strains. None of the indigenous silage isolates tested in this survey demonstrated specific antagonism via production of bacteriocinogenic compounds.

Occurrence of Listeria monocytogenes in Raw Milk in Nebraska

1988 ◽  
Vol 51 (11) ◽  
pp. 840-841 ◽  
Author(s):  
MICHAEL B. LIEWEN ◽  
MARK W. PLAUTZ
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Farms ◽  
Raw Milk ◽  
Storage Tanks ◽  
Biochemical Tests ◽  
Bulk Storage ◽  
Milk Samples ◽  
Listeria Species ◽  
Enrichment Procedure

Raw milk samples were obtained from bulk storage tanks of individual dairy farms in eastern Nebraska during February and July of 1986. One hundred different farms were tested during each period. One-tenth ml of each sample was plated directly onto McBride's Listeria Agar (MLA) and 30 ml was subjected to a four-week cold enrichment procedure. Suspect colonies from MLA were subjected to biochemical tests to confirm identity. Nine percent of all raw milk samples examined were determined to be positive for Listeria species after the cold enrichment procedure. Four percent contained L. monocytogenes and five percent contained L. innocua. Six percent and two percent of samples were found to contain L. monocytogenes in February and July respectively.

scholarly journals Application of a Multiplex Polymerase Chain Reaction Assay for the Simultaneous Confirmation of Listeria monocytogenes and Other Listeria Species in Turkey Sample Surveillance†

2002 ◽  
Vol 65 (5) ◽  
pp. 780-785 ◽  
Author(s):  
IRENE V. WESLEY ◽  
KAREN M. HARMON ◽  
JAMES S. DICKSON ◽  
ANN RAMOS SCHWARTZ
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Listeria Species ◽  
Multiplex Pcr Assay ◽  
Polymerase Chain

A multiplex polymerase chain reaction was developed to simultaneously identify Listeria monocytogenes and species of the genus Listeria. Two sets of primers were used, with the first amplifying a 938-bp region of the 16S rRNA gene that is highly conserved in all Listeria species and the second amplifying a 174-bp region of the listeriolysin (hlyA) gene of L. monocytogenes. Thus, isolates of Listeria spp. yield a single 938-bp product, whereas L. monocytogenes isolates yield both the 938-bp product and a 174-bp product. The specificity of the assay was verified with all six Listeria species and 11 serotypes of L. monocytogenes, as well as nonrelated bacteria. The multiplex PCR assay was used to determine the incidence of Listeria spp., especially L. monocytogenes, in mechanically separated turkey samples (n = 150 samples). L. monocytogenes strains were selected by using the University of Vermont two-step enrichment protocol and plating to selective Palcam agar. The multiplex PCR assay was used for verification of presumptive Listeria colonies. Approximately 38% of mechanically separated turkey samples (57 of 150) yielded L. monocytogenes; an additional 18% of these samples (27 of 150) harbored other Listeria spp. Fifty-one percent (29 of 57) of the L. monocytogenes isolates were of serogroup 1, 44% (25 of 57) were of serogroup 4, and 2% (1 of 57) were assigned to serogroups other than 1 and 4.

Enhanced Rapidity for Qualitative Detection of Listeria monocytogenes Using an Enzyme-Linked Immunosorbent Assay and Immunochromatography Strip Test Combined with Immunomagnetic Bead Separation

2008 ◽  
Vol 71 (4) ◽  
pp. 781-789 ◽  
Author(s):  
WON-BO SHIM ◽  
JIN-GIL CHOI ◽  
JI-YOUNG KIM ◽  
ZHENG-YOU YANG ◽  
KYU-HO LEE ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Meat ◽  
Immunomagnetic Bead ◽  
Listeria Species ◽  
Qualitative Detection ◽  
Meat Samples ◽  
Strip Test ◽  
Icg Strip

An enzyme-linked immunosorbent assay (ELISA), immunochromatography (ICG) strip test, and immunomagnetic bead separation (IMBS) system based on a monoclonal antibody were individually developed for the detection and isolation of Listeria monocytogenes in meat samples. The three methods showed a strong reaction with Listeria species and a weak reaction with Staphylococcus aureus. To increase the rapidity of L. monocytogenes detection, combinations of the ELISA and ICG strip test with the IMBS system (ELISA-IMBS and ICG-IMBS) were investigated. In comparative analyses of artificially inoculated meat and samples of processed meat, the ELISA and ICG strip test required 24 h of enrichment time to detect the inoculated meat samples with ≥ 1 × 102 CFU/10 g, whereas the ELISA-IMBS and ICG-IMBS required only 14 h of enrichment. Analyses of naturally contaminated meat samples (30 pork samples, 20 beef samples, 26 chicken samples, 20 fish samples, and 20 processed meat samples) performed by ELISA-IMBS, ICG-IMBS, and API kit produced similar results. The ELISA-IMBS and ICG-IMBS provide a more rapid assay than the individual ELISA and the ICG strip test and are appropriate for rapid and qualitative detection of L. monocytogenes (or Listeria species) in meat samples. With the ICG-IMBS, L. monocytogenes could be detected in meat samples within 15 h and the method has potential as a rapid, cost-effective on-site screening tool for the detection of L. monocytogenes in food samples and agricultural products at a minimum detection level of ~100 CFU/10 g.

scholarly journals INCIDÊNCIA DE Listeria monocytogenes EM QUEIJO DE COALHO REFRIGERADO PRODUZIDO INDUSTRIALMENTE

2003 ◽  
Vol 21 (2) ◽  
Author(s):  
MARIA AURINEIDE DE ABREU CASTELO BRANCO ◽  
EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO ◽  
MARIA DE FÁTTIMA BORGES ◽  
MARIA CRISTINA DELGADO DA SILVA ◽  
MARIA TEREZA DESTRO
Keyword(s):  
Listeria Monocytogenes ◽  
Water Activity ◽  
Rapid Test ◽  
Listeria Innocua ◽  
Biochemical Tests ◽  
Listeria Species ◽  
Ph Conditions ◽  
Ph Range ◽  
Visual Reading ◽  
The City

Avaliou-se a incidência de Listeria monocytogenes em queijo de coalho, produzido industrialmente e comercializado sob refrigeração na cidade de Fortaleza- CE (Brasil). Também foram avaliadas as condições de pH e de atividade de água nas amostras contaminadas com espécies de Listeria. Foram analisadas 84 amostras de queijo de coalho industrializado de diferentes marcas empregando o TECRA Listeria Visual Immunoassay (LISVIA) modificado. Das 84 amostras, 16 (19%) estavam contaminadas com Listeria monocytogenes, 5 (5,9%) com Listeria innocua e 1 (1,2%) com Listeria grayi. Listeria monocytogenes foi isolada na faixa de pH de 5,75 a 6,37 e em atividade de água entre 0,949 e 0,970. O TECRA LISVIA detectou a presença de Listeria spp. em 9 (10,7%) amostras. Todas as amostras positivas no teste rápido foram confirmadas por testes culturais e bioquímicos e em todas foi detectada a presença de Listeria monocytogenes. O plaqueamento das amostras negativas na leitura visual do teste rápido permitiu o isolamento de Listeria spp. em 8 amostras e em 7 foi detectada a presença de Listeria monocytogenes. INCIDENCE OF Listeria monocytogenes IN INDUSTRIALLY MANUFACTURED REFRIGERATED “COALHO” CHEESE Abstract Incidence of Listeria monocytogenes in “coalho” cheese industrially manufactured and commercialized in refrigerated temperature in the city of Fortaleza, Ceará (Brazil) was evaluated. Water activity and pH conditions in the contaminated samples with Listeria species were also evaluated. Samples (84) of industrialized “coalho” cheese of different brands were analyzed using the modified TECRA Listeria Visual Immunoassay (LISVIA). From 84 samples, 16 (19%) were contaminated with Listeria monocytogenes, 5 (5.9%) with Listeria innocua and 1 (1.2%) with Listeria grayi. Listeria monocytogenes was isolated in the pH range of 5.75 to 6.37 and in water activity between 0.949 e 0.970. The TECRA LISVIA detected the presence of Listeria spp. in 9 (10.7%) samples. All positives samples in the rapid test were confirmed by cultural and biochemical tests and in all samples the presence of Listeria monocytogenes was detected. The negative samples plating in the visual reading of the rapid test allowed the isolation of Listeria spp. in 8 samples and in 7 the presence of Listeria monocytogenes was detected.

Petite colony formation by Listeria monocytogenes and Listeria species grown on esculin-containing agar

1990 ◽  
Vol 36 (10) ◽  
pp. 697-703 ◽  
Author(s):  
Gregory R. Siragusa ◽  
L. A. Elphingstone ◽  
P. L. Wiese ◽  
S. M. Haefner ◽  
M. G. Johnson
Keyword(s):  
Listeria Monocytogenes ◽  
Key Words ◽  
Colony Formation ◽  
Ferric Iron ◽  
Hydrolysis Product ◽  
Parent Population ◽  
Listeria Species ◽  
Esculin Hydrolysis ◽  
Parent Stock ◽  
Agar Media

Several strains of Listeria species formed petite-sized colonies from parent stock cultures when grown on agar media containing 0.2 – 1% (w/v) esculin. This was observed in Listeria monocytogenes (7/22 strains), L. innocua (1/3), L. grayi (1/1), L. seeligeri (1/3), and L. welshimeri (1/1), but not in L. ivanovii (0/1) and L. murrayi (0/1). This phenomenon was only observed on agar media that contained esculin. All petite isolates had biotyping profiles identical to their larger, normal-sized counterpart isolates. Normal and petite-sized isolates from two L. monocytogenes strains, Scott A and V7, were pathogenic to immunosuppressed white mice. On media containing 0.5% (w/v) esculin + ferric iron, Listeria cultures produced colony diameters intermediate in size between those of normal and petite cultures. When pregrown in glucose broth, all petite isolates demonstrated visible β-glucosidase (esculinase) activity within 5 min, while the normal-sized isolates showed β-glucosidase activity only after at least 20–70 min. This evidence suggests that cells forming petite colonies are β-glucosidase constitutive variants within the parent population, while cells that form normal-sized colonies are inducible for β-glucosidase (esculinase) activity. A possible role for the esculin hydrolysis product, esculetin, in causing petite colony formation is discussed. Key words: Listeria species, petite colonies, esculinase, pathogenicity.

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