Health and Nutritional Benefits from Wild Probiotic Strains Isolated from Human Breast Milk, Zabady and Laben Rayb

Breast milk is well known as the abundant source of beneficial bacteria. A new alternative source of human probiotic origin from breast milk is in demand and currently of interest for both the functional food industry and biopharmaceuticals. The aim in this study was to investigate the anticancer and antioxidant efficacies of the new potential probiotics isolated from human breast milk. Three strains of lactic acid bacteria (LAB) have shown their potential probiotic criteria including antimicrobial activity, non-hemolytic property, and survival in acid and bile salt conditions. These strains showed high abilities on cell surface hydrophobicity, auto-aggregation, and co-aggregation. The genera identification by 16S rRNA sequencing and comparison revealed that they were Streptococcus salivarius BP8, S. salivarius BP156, and S. salivarius BP160. The inhibition of liver cancer cells (HepG2) and breast cancer cells (MCF-7) proliferation by these probiotic strains using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was 44.83–59.65 and 29.85–37.16%, respectively. The probiotic action mode was inducted via apoptotic mechanisms since they stimulate the liver and breast cancer cell death through DNA fragmentation and positive morphological changes by acridine orange (AO) and propidium iodide (PI) staining. The antioxidant activity of these probiotics in the form of intact cells, cell free supernatant (CFS), and heat-killed cells was evaluated by a 2,2–diphenyl–1–picrylhydrazyl (DPPH) assay, resulting in the scavenging activity rates of 16.93–25.43, 15.47–28.03, and 13.67–23.0%, respectively. These S. salivarius probiotic strains protected the L929 mouse fibroblasts against oxidative stress with very high survival rates at 94.04–97.77%, which was significantly higher (P < 0.05) than L-ascorbic acid at 75.89–78.67% in the control groups. The results indicated that S. salivarius BP8 and S. salivarius BP160 probiotic strains could be applied as functional foods or new alternative bioprophylactics for treating liver and breast cancers.

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Association of sCD14 in human breast milk with child atopic dermatitis: Results of the Ulm Birth Cohort Study and the Ulm SPATZ Health Study

10.1055/s-0037-1605765 ◽

2017 ◽

Author(s):

CA Logan ◽

J Weiss ◽

W Koenig ◽

V Walter ◽

H Brenner ◽

...

Keyword(s):

Atopic Dermatitis ◽

Cohort Study ◽

Breast Milk ◽

Birth Cohort ◽

Health Study ◽

Birth Cohort Study ◽

Human Breast Milk ◽

Human Breast

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Molecular Properties of Egg Allergen in Human Breast Milk and Its Physiological Function

Nippon Shokuhin Kagaku Kogaku Kaishi ◽

10.3136/nskkk.61.450 ◽

2014 ◽

Vol 61 (9) ◽

pp. 450-454

Author(s):

Hiroshi Narita

Keyword(s):

Breast Milk ◽

Physiological Function ◽

Molecular Properties ◽

Human Breast Milk ◽

Human Breast

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Organohalogen compounds in human breast milk from Republic of Buryatia, Russia

Environmental Pollution ◽

10.1016/j.envpol.2006.04.036 ◽

2007 ◽

Vol 146 (1) ◽

pp. 225-232 ◽

Cited By ~ 85

Author(s):

Oyuna V. Tsydenova ◽

Agus Sudaryanto ◽

Natsuko Kajiwara ◽

Tatsuya Kunisue ◽

Valeriy B. Batoev ◽

...

Keyword(s):

Breast Milk ◽

Human Breast Milk ◽

Human Breast ◽

Organohalogen Compounds

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Human breast milk suppresses the transcriptional regulation of IL-1β-induced NF-κB signaling in human intestinal cells

AJP Cell Physiology ◽

10.1152/ajpcell.00471.2003 ◽

2004 ◽

Vol 287 (5) ◽

pp. C1404-C1411 ◽

Cited By ~ 40

Author(s):

Ryoko Minekawa ◽

Takashi Takeda ◽

Masahiro Sakata ◽

Masami Hayashi ◽

Aki Isobe ◽

...

Keyword(s):

Breast Milk ◽

Pathogenic Bacteria ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Nuclear Factor Κb ◽

Protective Role ◽

Intestinal Cells ◽

Human Breast Milk ◽

Human Breast ◽

Activation Pathway

Neonatal necrotizing enterocolitis (NEC), which is a disease with a poor prognosis, is considered to be caused by the coincidence of intestinal ischemia-reperfusion injury and systemic inflammation due to the colonization of pathogenic bacteria. Interleukin (IL)-8, a proinflammatory cytokine, plays an important role in the pathophysiology of NEC. It was recently reported that IL-1β activates the IL-8 gene by regulating the transcriptional nuclear factor κB (NF-κB) signaling pathways in intestinal cells. The protective role of maternal milk in NEC pathogenesis has been reported in both human and animal studies. In this study, we show that human breast milk dramatically suppressed the IL-1β-induced activation of the IL-8 gene promoter by inhibiting the activation pathway of NF-κB. Moreover, we also show that human breast milk induced the production of IκBα. These results suggest that human breast milk could be protective and therapeutic in neonates with NEC by inhibiting the activation pathway of NF-κB.

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Comparison of Vitamin D and 25-Hydroxyvitamin D Concentrations in Human Breast Milk between 1989 and 2016–2017

Nutrients ◽

10.3390/nu13020573 ◽

2021 ◽

Vol 13 (2) ◽

pp. 573

Author(s):

Naoko Tsugawa ◽

Mayu Nishino ◽

Akiko Kuwabara ◽

Honami Ogasawara ◽

Maya Kamao ◽

...

Keyword(s):

Vitamin D ◽

Breast Milk ◽

Sun Exposure ◽

Stepwise Multiple Regression ◽

Human Breast Milk ◽

Vitamin D Status ◽

Human Breast ◽

Hydroxyvitamin D ◽

Lactating Mothers ◽

25 Hydroxyvitamin D

Background: Breast milk is considered the optimal source of nutrition during infancy. Although the vitamin D concentration in human breast milk is generally considered poor for infants, vitamin D in breast milk is an important source for exclusively breastfed infants. Increases in vitamin D insufficiency and deficiency in lactating mothers may reduce vitamin D concentrations in breast milk. This study aimed to compare vitamin D and 25-hydroxyvitamin D (25OHD) concentrations in breast milk collected in 1989 and 2016–2017 and simultaneously analyze them with liquid chromatography-tandem mass spectrometry (LC-MS/MS); the association between the lifestyle of recent lactating mothers (2016–2017) and vitamin D status in human breast milk was also evaluated. Method: Lactating mothers were recruited from three regions of Japan in 1989 (n = 72) and 2016–2017 (n = 90), and milk from 3–4 months was collected in summer and winter. The samples were strictly sealed and stored at −80℃ until measurement. Breast milk vitamin D and 25OHD concentrations were analyzed by LC-MS/MS. Vitamin D intake, sun exposure, and sunscreen use of the lactating mothers in 2016–2017 were assessed. Results: Both vitamin D and 25OHD concentrations in breast milk were higher in the summer regardless of the survey year. Significantly lower vitamin D and 25OHD concentrations were observed in 2016–2017 compared with 1989 in summer, but no survey year difference was observed in winter. The stepwise multiple regression analyses identified season, daily outdoor activity, and suntan in the last 12 months as independent factors associated with vitamin D3 concentrations. Conclusion: The results suggest that low vitamin D status in recent lactating mothers may have decreased vitamin D and 25OHD concentrations in breast milk compared with the 1980s. These results are helpful for developing public health strategies to improve vitamin D status in lactating mothers and infants.

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