scholarly journals EFFECT OF GROWING MEDIA AND NITROGEN SOURCES ON GROWTH, ALKALOIDS CONTENT AND SOME CHEMICAL CONSTITUENTS OF CATHARANTHUS ROSEUS PLANT

2009 ◽  
Vol 34 (6) ◽  
pp. 6763-6780
Author(s):  
A. Khalil ◽  
A. Gendy ◽  
E. Hamad ◽  
E. Ismail
Keyword(s):  
Catharanthus Roseus ◽  
Chemical Constituents ◽  
Nitrogen Sources ◽  
Growing Media

scholarly journals Comparative studies of Volatile Components of the essential Oil of leaves and flowers of Catharanthus roseus growing in Bangladesh by GC-MS analysis

2015 ◽  
Vol 3 (01) ◽  
pp. 06-10 ◽  
Author(s):  
Shahin Aziz ◽  
Koushik Saha ◽  
Nasim Sultana ◽  
Mala Khan ◽  
Katrun Nada ◽  
...  
Keyword(s):  
Essential Oil ◽  
Catharanthus Roseus ◽  
Chemical Constituents ◽  
Fatty Acid Esters ◽  
Volatile Fraction ◽  
Volatile Components ◽  
Ms Analysis ◽  
Analytical Tools ◽  
Acid Esters ◽  
Important Medicinal Plant

Catharanthus roseus is an important medicinal plant commonly known as Madagasker periwinkle. Traditionally different parts of its used in the treatment of various diseases (e.g. diabetes, menstrual regulators, hypertension, cancer and antigalactogogue etc.) in number of countries. The essential oil of leaves and flowers of Catharanthus roseus growing in Bangladesh were investigated by gas chromatography coupled to mass spectrophotometery (GC-MS) to determined the chemical composition of the volatile fraction and identify their chemotypes. Quantification of active principles through analytical tools is essential for establishing the authenticity and credibility. Extraction by steam distillation and analysis with GC-MS have been shown to be valuable tools for the identification of the constituents of essential oil of leaves and flowers of Catharanthus roseus and can provide a useful guide about the variation of the components. The main objective of the present study was focused on identification and quantification of chemical constituents present in the essential oil of leaves and flowers of Catharanthus roseus by GC-MS methods. 24 compounds consisting (95.99%) of the total for the case of leaves were identified. And total 10 compounds consisting (98.20%) of the total for the case of flowers of Catharanthus roseus were identified by GC-MS analysis in Bangladesh. For the case of leaves the identified components were : terpenoids (75.41%) , alkanes (6.0%) , aldehydes (5.5%), fatty acids (3.6 %), ketones (3.2%), alcohol (2.28%) respectively. For the case of flowers the identified components fatty acid esters (98.20) % respectively.

scholarly journals Minimizing Irrigation and Fertilization in Greenhouse-grown Flowering Vinca

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 533C-533
Author(s):  
Karen L. Panter ◽  
Amy M. Briggs ◽  
Michael J. Roll ◽  
Steven E. Newman
Keyword(s):  
Catharanthus Roseus ◽  
Slow Release ◽  
Optimum Growth ◽  
Irrigation Systems ◽  
Growing Media ◽  
Waste Tires ◽  
Irrigation Methods ◽  
Growing Medium

The objective of this study was to determine which combination of three types of irrigation systems, three fertilization method, and four growing media produced optimum growth of flowering vinca, Catharanthus roseus. Irrigation systems used included ebb-and-fl ood, drip, and pulse; fertilization methods included slow release, prepackaged, and custom mixed; and the four growing media were peatmoss:perlite:vermiculite (1:1:1, by volume), peatmoss:rockwool (1:1, by volume), and 0.6-cm diameter shredded rubber or fabric from waste tires: vermiculite:peatmoss (1:1:2, by volume). Four replications of five plants each were used in each of the 36 treatment combinations. Plants were potted 29 and 30 May 1996 in 10-cm containers, grown for 10 weeks, and harvested 6 Aug. 1996. The drip-irrigated benches were irrigated once per day for 15 s. Pulse-irrigated benches were watered twice per day for 6 s. This resulted in the drip- and pulse-irrigated plants receiving a similar volume of water daily. Ebb-and-fl ood benches were filled once per day with drainage occurring 15 min after filling. Ending plant heights and dry weights indicated that those plants in the prepackaged fertilizer/drip or ebb-and-fl ood irrigation/shredded tire fiber growing medium were comparable to plants grown in the peatmoss:rockwool medium with the same fertilizer and irrigation methods.

Changes in the activity of the enzymes involved in nitrogen metabolism in Catharanthus roseus depending on different nitrogen sources

1986 ◽  
Vol 64 (9) ◽  
pp. 2052-2056
Author(s):  
Victor Manuel Loyola-Vargas ◽  
Irene Gómez ◽  
Ma. Eugenia López ◽  
Jorge Reyes ◽  
Miriam Fierro ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nitrate Reductase ◽  
Catharanthus Roseus ◽  
Nitrite Reductase ◽  
Leaf Explants ◽  
Nitrogen Sources ◽  
Water Control ◽  
Whole Plants ◽  
Leaf Nitrate

The activities of the enzymes nitrate reductase (EC 1.6.6.1) and nitrite reductase (EC 1.7.99.3) were measured in the leaves and roots of whole plants of Catharanthus roseus grown in different nitrogen sources: water (control), 20 mM KNO3, 2 mM NH4Cl, and a mixture of nitrate and ammonium. The activities of these enzymes were also measured in leaf explants incubated in the same nitrogen sources. The results indicate that nitrate reductase and nitrite reductase in leaves behaved very differently from those in roots. Activity of leaf nitrate reductase measured in vitro decreased with nitrate and increased with ammonium, while in the root, it only decreased when the mixture of both nitrogen sources was employed. In contrast, nitrite reductase from roots was modified by the nitrogen source while the enzyme from the leaves was not. The amino acid pool was increased by all of the nitrogen sources in both whole plants and explants.

scholarly journals Combined effects of NPK fertilizer with foliar application of benzyladenine or gibberellic acid on Dracaena marginata ‘Bicolor’ grown in different potting media

2020 ◽  
Vol 26 (4) ◽  
pp. 545-561
Author(s):  
Hossam Ahmed Ashour ◽  
Asmaa Bader Eldeen El-Attar ◽  
Mohamed Mahmoud Abdel Wahab
Keyword(s):  
Gibberellic Acid ◽  
Foliar Application ◽  
Growth Parameters ◽  
Chemical Constituents ◽  
Peat Moss ◽  
Npk Fertilizers ◽  
Growing Media ◽  
Economic Production ◽  
Total Carbohydrates ◽  
Npk Fertilizer

Abstract A pot experiments was carried out to evaluate the influence of growing media and combined treatments of NPK with either benzyladenine or gibberellic acid on growth, chemical constituents and anatomical structure of Dracaena marginata ‘Bicolor’. The plants were grown in two growing media; peat-moss, peat-moss+ sand (1:1, v v-1), received monthly NPK fertilizers (2 and 4 g pot-1) combined with either of benzyl adenine (BA) at 100 and 150 ppm or gibberellic acid (GA3) at 150 and 250 ppm, while the control plants received no treatments. As general, the results indicated that, peat- moss was superior to peat-moss+ sand medium on increasing most of vegetative growth parameters in terms of plant height, number of leaves/plant, leaf area, root length, as well as fresh and dry weights of leaves, stems and roots/plant, besides some macro elements represented in N, P, K, Ca and Mg% in both leaves and stems. While plants grown in peat-moss+ sand possessed significantly higher contents of total chlorophylls, total carbohydrates, Cu, Fe, Mn, Zn and B than those grown in peat- moss alone. Plants received combined NPK with either BA or GA3 resulted in significant increases in most of morphological and chemicals content over the control plants and it was outstanding that, GA3 was more effective than BA when they were combined with NPK. It can be concluded that for the highest quality, quantity growth and economic production of Dracaena marginata ‘Bicolor’, the plants could be grown in a medium of peat-moss and supplied monthly with NPK fertilizer at 2 g plant-1 along with foliar sprayed with 250 ppm GA3.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

Sem and X-Ray Analysis of Renal and Biliary Calculi

1976 ◽  
Vol 34 ◽  
pp. 306-307
Author(s):  
R. J. Narconis ◽  
G. L. Johnson
Keyword(s):  
Chemical Constituents ◽  
Natural State ◽  
X Ray Diffraction ◽  
Chemical Methods ◽  
X Ray ◽  
Additional Dimension ◽  
Biliary Calculi ◽  
Calculus Formation ◽  
Scanning Electron

Analysis of the constituents of renal and biliary calculi may be of help in the management of patients with calculous disease. Several methods of analysis are available for identifying these constituents. Most common are chemical methods, optical crystallography, x-ray diffraction, and infrared spectroscopy. The application of a SEM with x-ray analysis capabilities should be considered as an additional alternative.A scanning electron microscope equipped with an x-ray “mapping” attachment offers an additional dimension in its ability to locate elemental constituents geographically, and thus, provide a clue in determination of possible metabolic etiology in calculus formation. The ability of this method to give an undisturbed view of adjacent layers of elements in their natural state is of advantage in determining the sequence of formation of subsequent layers of chemical constituents.

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