Application of Sulfate-Reducing and Sulfide-Oxidizing Bacterial Symbiosis for Wastewater Treatment

1995 ◽  
Vol 30 (2) ◽  
pp. 305-324 ◽  
Author(s):  
V. Tare ◽  
P.C. Sabumon
Keyword(s):  
Organic Matter ◽  
Microbial Population ◽  
Domestic Wastewater ◽  
Growth Conditions ◽  
Sulfate Reducing ◽  
Bacterial Symbiosis ◽  
Scale Models ◽  
Microbial System ◽  
Set Up ◽  
Reducing Bacteria

Abstract This investigation attempted to advance the state of the art of the process which utilizes the symbiotic relationship between the sulfate-reducing bacteria (SRB) and sulfide oxidizing bacteria (SOB) for degradation of organic matter present in wastewater. Major emphasis has been on the development of the desired microbial system without any external seed and comparative evaluation of the two types of multistage reversing flow bioreactor (MRB) systems. Biological vessels (BVs) in the MRB systems simulate conditions which correspond to configurations described as upflow sludge blanket and stationary fixed film. Two bench-scale models – one designed to achieve self granulation of sludge (SGS), and the second designed to promote growth of SRB/SOB on additional nonreactive surface – were set up and operated over a period of 4 months. Domestic wastewater supplemented with organic matter from sugar cane molasses was used as feed to develop the desired microbial population. Several visual and microscopic observations confirmed the presence of a significant number of SRB and SOB in all the biological vessels. Results indicated that it is possible to develop SGS and a microbial population of SRB and SOB which could attach to the nonreactive surface without any external seeding. Domestic wastewater could serve as a source of these organisms. Immobilized growth conditions and suspended growth conditions in BVs yield similar results in terms of organic matter utilization. The empirical formula for MRB biomass can be expressed as C11O12H36N5S.

scholarly journals Simultaneous Degradation of Waste Phosphogypsum and Liquid Manure from Industrial Pig Farm by a Mixed Community of Sulfate-Reducing Bacteria

2010 ◽  
Vol 59 (4) ◽  
pp. 241-247 ◽  
Author(s):  
MARZENNA RZECZYCKA ◽  
ANTONI MIERNIK ◽  
ZDZISLAW MARKIEWICZ
Keyword(s):  
Organic Matter ◽  
Synthetic Medium ◽  
Sulfate Reducing Bacteria ◽  
Pig Manure ◽  
Liquid Manure ◽  
Sulfate Reducing ◽  
Pig Farm ◽  
Set Up ◽  
Reducing Bacteria ◽  
Simultaneous Degradation

The utilization of pig manure as a source of nutrients for the dissimilatory reduction of sulfates present in phosphogypsum was investigated. In both types of media used (synthetic medium and raw pig manure) increased utilization of sulfates with growing COD/SO4(2-)ratio in the medium was observed. The percent of sulfate reduction obtained in synthetic medium was from 18 to 99%, whereas the value for cultures set up in raw liquid manure was from 12% (at COD/SO4(2-) of 0.3) up to as high as 98% (at COD/SO4(2-) equal 3.80). Even with almost complete reduction of sulfates the percent of COD reduction did not exceed 55%. Based on the results obtained it was concluded that the effectiveness of removal of sulfates and organic matter by sulfate-reducing bacteria (SRB) depends to a considerable degree on the proportion between organic matter and sulfates in the purified wastewaters. The optimal COD/SO4(2-)ratio for the removal oforganic matter was between 0.6 and 1.2 whereas the optimal ratio for the removal of sulfates was between 2.4 and 4.8.

Novel application of sulphur metabolism in domestic wastewater treatment

1995 ◽  
Vol 22 (6) ◽  
pp. 1217-1223 ◽  
Author(s):  
Saibal Kumar Basu ◽  
J. A. Oleszkiewicz ◽  
Takashi Mino
Keyword(s):  
Organic Matter ◽  
Domestic Wastewater ◽  
Cod Removal ◽  
Sulphur Metabolism ◽  
Principal Mechanism ◽  
Organic Matter Removal ◽  
Sulphate Reducing Bacteria ◽  
Bacterial Symbiosis ◽  
Relationship Of ◽  
Reducing Bacteria

The feasibility of utilizing the symbiotic relationship of bacteria related to sulphur metabolism was investigated for organic matter removal in a sludge blanket type reactor. The microaerophilic upflow sludge bed reactor (MUSB) relies on the interaction between sulphate reducing bacteria (SRB) and microaerophilic sulphide oxidizing bacteria (SOB), Beggiatoa, for organic matter removal. A five-stage MUSB reactor with a volume of 173 L was operated for 120 days at three hydraulic loadings. The efficiency of the process to remove total-COD (T-COD), filtered-COD (F-COD), and suspended solids (SS) depended on the hydraulic loading. Maximum removals of T-COD, F-COD, and SS were 92%, 94%, and 87% respectively at hydraulic retention time (HRT) of 4.5 h. At a low HRT of 2.5 h, a F-COD removal efficiency of 89% could still be achieved. Although no granulation was observed, a dense flocculated biomass developed which exhibited very good settleability (SVI = 16 mL/g). While the effluent SS increased at the lower HRTs of 3.5 and 2.5 h, the system still operated effectively without a secondary sedimentation tank. Sulphate balance, batch studies, and microscopic examinations indicated the proliferation of SRB and SOB, Beggiatoa, in the biological vessels. Although some COD removal took place by aerobic metabolism in the aeration vessels, sulphur metabolism appears to be the principal mechanism responsible for organic matter removal in the MUSB process. Key words: bacterial symbiosis, sulphur metabolism, microaerophilic upflow sludge bed reactor, sulphate reducing bacteria, sulphide oxidizing bacteria, Beggiatoa.

Studies on Corrosion Behavior of Q235 Steel by Iron Bacteria, Sulfate-Reducing Bacteria and Total General Bacteria in Sedimentary Water of Storage Tank

2011 ◽  
Vol 337 ◽  
pp. 281-284
Author(s):  
Dong Sheng Chen ◽  
Yong Zhang Zhou ◽  
Min Liu ◽  
Kai Wei Guo ◽  
Wu Ji Wei
Keyword(s):  
Corrosion Behavior ◽  
Storage Tank ◽  
Corrosion Current ◽  
Sulfate Reducing Bacteria ◽  
Steel Electrode ◽  
Q235 Steel ◽  
Iron Bacteria ◽  
Sulfate Reducing ◽  
Microbial System ◽  
Reducing Bacteria

The corrosion behavior of Q235 steel by Iron Bacteria (IB), Sulfate-reducing Bacteria (SRB) and Total General Bacteria (TGB) in sedimentary water of storage tank from an aromatics plant was investigated mainly by static hanging piece method, potentiodynamic polarization curve and hysteresis loop method. The results showed that the interaction of IB, SRB and TGB accelerated the corrosion rate of Q235 steel. The corrosion current density of Q235 steel electrode in IB, SRB and TGB solution was higher than that in the sterile solution, and the corrosion potential shifted in negative direction. IB, SRB and TGB reduced the corrosion resistance of Q235 steel. The corrosion of Q235 steel in the mixture of IB, SRB and TGB was more serious than in a single microbial system. The presence of IB, SRB and TGB made the pitting occur easily.

scholarly journals Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue

2020 ◽  
Vol 17 (8) ◽  
pp. 2715 ◽  
Author(s):  
Aung Kyaw Phyo ◽  
Yan Jia ◽  
Qiaoyi Tan ◽  
Heyun Sun ◽  
Yunfeng Liu ◽  
...  
Keyword(s):  
Organic Matter ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Sulfate Reducing Bacteria ◽  
Sulfide Minerals ◽  
Sulfate Reducing ◽  
Heap Bioleaching ◽  
Waste Rocks ◽  
Sulfur Oxidizing ◽  
Reducing Bacteria

Mining waste rocks containing sulfide minerals naturally provide the habitat for iron- and sulfur-oxidizing microbes, and they accelerate the generation of acid mine drainage (AMD) by promoting the oxidation of sulfide minerals. Sulfate-reducing bacteria (SRB) are sometimes employed to treat the AMD solution by microbial-induced metal sulfide precipitation. It was attempted for the first time to grow SRB directly in the pyritic heap bioleaching residue to compete with the local iron- and sulfur-oxidizing microbes. The acidic SRB and iron-reducing microbes were cultured at pH 2.0 and 3.0. After it was applied to the acidic heap bioleaching residue, it showed that the elevated pH and the organic matter was important for them to compete with the local bioleaching acidophiles. The incubation with the addition of organic matter promoted the growth of SRB and iron-reducing microbes to inhibit the iron- and sulfur-oxidizing microbes, especially organic matter together with some lime. Under the growth of the SRB and iron-reducing microbes, pH increased from acidic to nearly neutral, the Eh also decreased, and the metal, precipitated together with the microbial-generated sulfide, resulted in very low Cu in the residue pore solution. These results prove the inhibition of acid mine drainage directly in situ of the pyritic waste rocks by the promotion of the growth of SRB and iron-reducing microbes to compete with local iron and sulfur-oxidizing microbes, which can be used for the source control of AMD from the sulfidic waste rocks and the final remediation.

scholarly journals The Self Ignition of Fiberglass Lines

2014 ◽  
Vol 39 ◽  
pp. 1-9
Author(s):  
Moussa Bounoughaz ◽  
Noura Touabi
Keyword(s):  
Microbial Population ◽  
Bacterial Species ◽  
Methanogenic Bacteria ◽  
Oil Field ◽  
Microbiological Analysis ◽  
High Concentration ◽  
Sulfate Reducing ◽  
Maintenance Work ◽  
Reducing Bacteria

The fiberglass pipes were used for transportation of water in order to maintain the pressure in the oil field of Zarzaïtine (Region of In Amenas located in the south of Algeria). During the maintenance work in summer, a season well known for its extensive heat, the deposits contained in the pipe, and after its contact with the atmosphere, have caused a smoke and it was followed by a fire that ignited a portion of the pipe. To give an answer to the causes of this phenomenon, we have first incriminated the role of specific bacterial species and therefore we have made a microbiological analysis of the deposits collected from the site of the incident. The obtained results revealed the presence of a heterogeneous microbial population with a high concentration level of sulfate-reducing bacteria (SRB), methanogenic bacteria (MB), yeasts and fungi. The interaction between the different species of bacteria and the organic matter contained in the deposits has generated the formation of methane which under the influence of the great heat burnt and the fire caused the ignition of the fiberglass line.

Introduction

2021 ◽  
pp. 1-20
Author(s):  
David Rickard
Keyword(s):  
Organic Matter ◽  
High Temperature ◽  
Water Column ◽  
Peat Bog ◽  
Chemical Analyses ◽  
Limited Distribution ◽  
Sulfate Reducing ◽  
Technological Advances ◽  
Framboid Formation ◽  
Reducing Bacteria

Framboids are microscopic subspherical clusters of equant and equidimensional microcrystals. They overwhelmingly consist of the mineral pyrite, cubic FeS2. There are about 1030 framboids on Earth and they are forming at a rate of about 1014 per second. They may be the most abundant mineral texture on Earth. Framboids are especially concentrated in sediments, although they are also to be found in the water column and in high temperature systems. The oldest framboids are possibly 2.9 Ga and they are found in all geologic periods from that time. The first framboids were described in 1885 from a peat bog, and the term framboid was coined in 1935. They have fascinated researchers ever since, not least because a substantial fraction of them display astonishing regular microarchitectures where their constituent microcrystals are geometrically ordered. Understanding of the nature of framboids has paralleled technological advances in microscopy, structural and chemical analyses, and computing. The sulfur in sedimentary framboids is almost exclusively sourced from sulfate-reducing bacteria, and the idea that framboids were fossil microorganisms was first propounded in 1923. Subsequently, the limited distribution of organic matter in framboids, its absence in hydrothermal framboids, and inorganic framboid syntheses showed that organisms were not necessary for framboid formation.

Influence of Sulfate-Reducing Bacteria on the Mobility of Arsenic in Soils an "In Vitro" Study

2013 ◽  
Vol 825 ◽  
pp. 548-551 ◽  
Author(s):  
Lillian Rabelo Lopes ◽  
Mônica Cristina Teixeira
Keyword(s):  
Organic Matter ◽  
Soil Samples ◽  
Adsorptive Capacity ◽  
Gold Mines ◽  
Sulfate Reducing ◽  
Bacterial Inoculum ◽  
Group 2 ◽  
Reducing Bacteria ◽  
As Solubility

Ouro Preto/MG/Brazil soils are rich in arsenic containing minerals that once solubilized may contaminate water or food. Arsenic (As) is toxic if ingested or inhaled. Microorganisms and organic matter plays an important role in the dynamics of As in soils and sediments affecting its mobilization. Aims: to study the mobility of arsenic in the presence of organic matter and sulfur reducing bacteria (SRB) and also to obtain some As resistant bacterial cultures. Materials: Soil samples were collected from abandoned gold mines named Old Mine, Chico Rei and Santa Rita. As content in solid samples were in a range of 465 to 1829 mg Kg-1. Soil samples (5 g) were mixed with 35 ml of 2.5 M, CaCl2, stirred (5 min) and allowed to rest at 21.0°C for 1, 30, 60, 90 and 120 days. Other set of experiments were prepared following the previous procedure with some modifications: (i) group 1, flasks with bacterial inoculum (4 ml) from an enrichment of 5 g of soil and 45 ml of liquid medium B Postgate, pH 7.0, incubated at 35°C under anaerobic conditions; (ii) group 2, flasks with (5 g) of organic matter with As (III) adsorptive capacity (powdered chicken feathers - PCF), and (iii) group 3, flasks containing bacterial inoculum and organic matter. Results: As solubility was inversely proportional to time and depends on Fe and Mn contents. The influence of microorganisms on As immobilization was more relevant than the presence of organic matter. Surprisingly, in some cases, As solubility enhanced in the presence of PCF besides its As adsorptive capacity. One microbial consortium adapted to the culturing at pH 5.0 was obtained and this is an interesting feature considering the acid pH of the studied arsenic soils. Indigenous bacteria phenotypically similar to SRB may contribute to As immobilization in natural or impacted environment.

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