Potassium and Magnesium Requirements for Enhanced Biological Phosphorus Removal from Wastewater

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2203-2206 ◽  
Author(s):  
L. F. Rickard ◽  
S. A. McClintock
Keyword(s):  
Phosphorus Removal ◽  
Test Phase ◽  
Synthetic Wastewater ◽  
Molar Ratio ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
System A ◽  
Calcium Iron ◽  
Biological Phosphorus

The role of potassium (K) and magnesium (Mg) in enhanced biological phosphorus removal (EBPR) by activated sludge was studied using a bench-scale continuous-flow A/O system. A synthetic wastewater containing all the nutrients required for EBPR was used as the influent feed for the control phase of the experiment. The influent feed to the test phase of the experiment was changed to totally limit specific cations. The results clearly indicated that both K and Mg were absolutely required for successful EBPR. Failure of EBPR occurred when either K or Mg were eliminated from the influent. The molar ratio of K:P during anaerobic release and aerobic uptake was observed to be 0.22 mol/mol, while Mg:P was 0.30 mol/mol. Calcium was not required for successful EBPR. Neither calcium, iron, nor sodium were co-transported with phosphorus during release and uptake.

A flux-based stoichiometric model of enhanced biological phosphorus removal metabolism

1998 ◽  
Vol 37 (4-5) ◽  
pp. 609-613
Author(s):  
J. Pramanik ◽  
P. L. Trelstad ◽  
J. D. Keasling
Keyword(s):  
Phosphorus Removal ◽  
Reaction Network ◽  
Bacterial Biomass ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Stoichiometric Model ◽  
Aerobic Reactor ◽  
Complete Set ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) in wastewater treatment involves metabolic cycling through the biopolymers polyphosphate (polyP), polyhydroxybutyrate (PHB), and glycogen. This cycling is induced through treatment systems that alternate between carbon-rich anaerobic and carbon-poor aerobic reactor basins. While the appearance and disappearance of these biopolymers has been documented, the intracellular pressures that regulate their synthesis and degradation are not well understood. Current models of the EBPR process have examined a limited number of metabolic pathways that are frequently lumped into an even smaller number of “reactions.” This work, on the other hand, uses a stoichiometric model that contains a complete set of the pathways involved in bacterial biomass synthesis and energy production to examine EBPR metabolism. Using the stoichiometric model we were able to analyze the role of EBPR metabolism within the larger context of total cellular metabolism, as well as predict the flux distribution of carbon and energy fluxes throughout the total reaction network. The model was able to predict the consumption of PHB, the degradation of polyP, the uptake of acetate and the release of Pi. It demonstrated the relationship between acetate uptake and Pi release, and the effect of pH on this relationship. The model also allowed analysis of growth metabolism with respect to EBPR.

scholarly journals Layered Extraction and Adsorption Performance of Extracellular Polymeric Substances from Activated Sludge in the Enhanced Biological Phosphorus Removal Process

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3358 ◽  
Author(s):  
Daxue Li ◽  
Hailing Xi
Keyword(s):  
Activated Sludge ◽  
Functional Groups ◽  
Phosphorus Removal ◽  
Extracellular Polymeric Substances ◽  
Cation Exchange Resin ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Adsorption Performance ◽  
Biological Phosphorus

A large amount of phosphorus was found in the extracellular polymeric substances (EPS) of activated sludge used in enhanced biological phosphorus removal (EBPR), so the role of EPS and extracellular phosphorus in EBPR should not be neglected. The composition and properties of tightly bound EPS (TB-EPS) and loosely bound EPS (LB-EPS) were significantly different, and it was necessary to study the adsorption performance of EPS through the fractionating of activated sludge into LB-EPS, TB-EPS and microbial cells. In this study, the adsorption performance of LB-EPS and TB-EPS for phosphate was explored by extracting LB-EPS and TB-EPS via sonication and cation exchange resin (CER), respectively. The results indicated that the sonication-CER method was an efficient and reliable extraction method for EPS with a synergistic effect. The performance of EPS in the adsorption/complexing of phosphate was excellent because of its abundant functional groups. Specifically, the type and content of metal elements and functional groups in TB-EPS were much greater than those in LB-EPS, which led to the key role of TB-EPS in the adsorption/complexing of phosphate. Finally, a metabolic model for EBPR with consideration of the adsorption performance of LB-EPS and TB-EPS was proposed.

scholarly journals Analysis of Polyphosphate during the Enhanced Biological Phosphorus Removal process using Fourier Transform Infrared (FTIR) spectroscopy

2020 ◽  
Vol 145 ◽  
pp. 02077
Author(s):  
Rong wang ◽  
Yuanyuan Ma ◽  
Di Zhang ◽  
Weihua Li
Keyword(s):  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Phosphorus Removal ◽  
Rapid Determination ◽  
Principal Component ◽  
Fourier Transform Infrared ◽  
Synthetic Wastewater ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus

Two enhanced biological phosphorus removal (EBPR) reactors were operated in synthetic wastewater with different Chemical Oxygen Demand/Phosphorus (COD/P) ratios. The sludge samples at different sample times were lyophilized and characterized by Fourier Transform Infrared (FTIR) spectroscopy. Results showed that the infrared spectral curves from two reactors were similar but the intensities were different at specific wavenumbers. The spectral intensities at 1260cm-1 and 890cm-1 of the sludge samples from the reactor at low COD/P ratios were relatively stronger, but those of the reactor at high COD/P ratios were relatively weaker. The principal component projection plot of FTIR spectra analyzed with principal components analysis (PCA) showed that the sludge samples at the same COD/P ratios assembled together implicating the principal component projection plot could discriminate the sludge samples from different reactor. The infrared peak near 890cm-1 was separated and integrated with Gaussian peak fitting method. The integrated areas were correlated to the polyphosphate content in the sludge. Results showed that the prediction values were in good accordance with those determined by chemical method. This work provides a new method for the recognition of polyphosphate in sludge and provides a basis for the rapid determination of polyphosphate.

The competition between PAOs (phosphorus accumulating organisms) and GAOs (glycogen accumulating organisms) in EBPR (enhanced biological phosphorus removal) systems at different temperatures and the effects on system performance

2003 ◽  
Vol 47 (11) ◽  
pp. 1-8 ◽  
Author(s):  
U.G. Erdal ◽  
Z.K. Erdal ◽  
C.W. Randall
Keyword(s):  
Phosphorus Removal ◽  
System Performance ◽  
Glycogen Metabolism ◽  
Reaction Rates ◽  
Synthetic Wastewater ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Different Temperatures ◽  
High Concentrations ◽  
Biological Phosphorus

It is well known and firmly established that the rate of chemical and biochemical reactions slow down as temperature decreases. Nevertheless, several studies have reported that the efficiency of enhanced biological phosphorus removal (EBPR) improves as temperature decreases. However, several recent studies have reported that EBPR reaction rates decrease with temperature decrease in accordance with the Arrhenius relationship. This study was designed to more thoroughly investigate this controversy using two UCT plants fed with a synthetic wastewater consisting primarily of acetate as the COD form, and a small amount of supplemental yeast extract. Experiments were performed over temperatures ranging from 5 to 20°C. The results showed that, even though the kinetic rates decrease as temperature decreases, EBPR systems perform better at colder temperatures. The reason for better system performance is apparently related to reduced competition for substrate in the non-oxic zones, which results in an increased population of PAOs and, thus, greater EBPR efficiency. The proliferation of PAOs apparently occurs because they are psychrophilic whereas their competitors are not. The experiments showed that the EBPR sludges accumulated high concentrations of both PHA and glycogen at 20°C, but accumulated more PHA and much less glycogen at 5°C. Although the results could be interpreted as the result of changes in the PAO-GAO competition, Mann-Whitney non-parametric comparisons of transmission electron microscopy examinations revealed no indication of the presence of GAOs population under any temperature conditions. Regardless, mass balances of the glycogen data showed that the involvement of glycogen is less at cold temperature, even though EBPR was greater. Unlike current EBPR models (e.g. Mino model), the results suggest that glycogen metabolism is not a precursor for EBPR biochemistry. The results also indicate that temperature not only may cause selective pressure on the dominant organisms, but also may force them to use a different metabolic pathway as temperature decreases.

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