Comparative effectiveness of UV wavelengths for the inactivation of Cryptosporidium parvum oocysts in water

2001 ◽  
Vol 43 (12) ◽  
pp. 171-174 ◽  
Author(s):  
K. G. Linden ◽  
G. Shin ◽  
M. D. Sobsey

Cryptosporidium parvum oocysts in water were exposed to distinct wavelength bands of collimated beam ultraviolet (UV) radiation across the germicidal UV wavelength range (210-295 nm) that were emitted from a medium pressure (MP) mercury vapour lamp. The dose of UV radiation transmitted though each narrow bandpass filter was measured utilising potassium ferrioxalate actinometry. Oocyst infectivity was determined using a cell culture assay and titre was expressed as an MPN. The log10 inactivation for each band of radiation was determined for a dose of 2 mJ/cm2. Doses from all wavelengths between 250-275 nm resulted in approximately 2 log10 inactivation of Cryptosporidium parvum oocyst infectivity while doses with wavelengths higher and lower than this range were less effective. Because polychromatic radiation from MP UV lamps had about the same germicidal activity between the wavelengths of 250-275 nm for inactivation of oocyst infectivity, there was no unique advantage of MP UV over low pressure (LP) UV except for the simultaneous delivery of a wide range of germicidal wavelengths.

The measurement of the intensity of a source of light is, it is well known, a somewhat unsatisfactory process. The eye cannot estimate light intensity; it can only tell when the illumination of two adjacent surfaces is equal. If, for example, we desire to measure the intensity of a metal filament lamp, we compare it with a Hefner lamp and say that the intensities are inversely as the squares of the distances from the photometer head, when equal illumination is obtained. In strictness, however, this method is applicable only when the colours of the two sources, or more accurately when the distribution of energy in the spectra of the two sources, is exactly the same; for the relative luminosity of the different colours of a spectrum varies with the intensity of that spectrum. Abney has two well-known curves illustratinghis. One, which represents the relative luminosity of the different colours of a spectrum at ordinary intensity, has a maximum in the orange; the other which is for a spectrum with the same distribution of energy, but with an intensity of less than 1/100 candle-foot, has its maximum in the green. If, therefore, we have an extremely long photometer bench, and an experimenter with normal colour vision compares the intensities of the metal filament lamp and the Hefner lamp, at first placing the Hefner lamp one foot from the photometer head and afterwards placing it more than 100 ft. from the latter, he should not obtain the same result both times. In the first case, owing to the reddish tint of the Hefner lamp, the intensity of the metal filament lamp should appear less. If, again, a second observer, whose colour vision is slightly abnormal, compares the lamps at the first distance, he gets a third result. Of course the difficulty does not arise in practice, because the sources to be compared have usually the same colour and the illumination of the field of the photometer does not vary over a wide range. Still, a standard unit of light should meet all conceivable cases, and we are at present unable to state satisfactorily in terms of our standards, once for all, the candle power of, for example, a mercury vapour lamp. In order to be definite we must specify, first of all, normal colour vision on the part of the observer, and then we must state the illumination of the fields he compares. It is, of course, the Purkinje effect, the change from rod to cone vision, that causes all this trouble. And it is precisely within the range of illumination in common use, 1 to 100 metre-candles, that this change from rod to cone vision takes place.


2001 ◽  
Vol 67 (7) ◽  
pp. 3029-3032 ◽  
Author(s):  
Gwy-Am Shin ◽  
Karl G. Linden ◽  
Michael J. Arrowood ◽  
Mark D. Sobsey

ABSTRACT Because Cryptosporidium parvum oocysts are very resistant to conventional water treatment processes, including chemical disinfection, we determined the kinetics and extent of their inactivation by monochromatic, low-pressure (LP), mercury vapor lamp UV radiation and their subsequent potential for DNA repair of UV damage. A UV collimated-beam apparatus was used to expose suspensions of purifiedC. parvum oocysts in phosphate-buffered saline, pH 7.3, at 25�C to various doses of monochromatic LP UV. C. parvuminfectivity reductions were rapid, approximately first order, and at a dose of 3 mJ/cm2 (=30 J/m2), the reduction reached the cell culture assay detection limit of ∼3 log10. At UV doses of 1.2 and 3 mJ/cm2, the log10 reductions of C. parvum oocyst infectivity were not significantly different for control oocysts and those exposed to dark or light repair conditions for UV-induced DNA damage. These results indicate that C. parvum oocysts are very sensitive to inactivation by low doses of monochromatic LP UV radiation and that there is no phenotypic evidence of either light or dark repair of UV-induced DNA damage.


2000 ◽  
Vol 41 (7) ◽  
pp. 127-134 ◽  
Author(s):  
M. J. Casteel ◽  
M. D. Sobsey ◽  
M. J. Arrowood

Alternative disinfectants of water and wastewater are needed because conventional chlorination is ineffective against C. parvum oocysts. Reliable indicators of disinfection efficacy against C. parvum also are needed. Mixedoxidants (MO) electrochemically generated from brine were evaluated in batch disinfection experiments for inactivation of C. parvum oocysts and Cl. perfringensspores in both oxidant demand-free (ODF) water and treated wastewater. Coliphage MS2 and Escherichia coli B were also tested under some conditions. C. parvum oocyst infectivity was quantified by cell culture assay, and the dyes DAPI (4′,6-diamidino-2-phenylindole) and propidium iodide (PI) were used to assess oocyst viability in wastewater experiments. In treated wastewater dosed with 10–13 mg/L MO, inactivation after 90 minutes was about 3 log10 for C. parvum and about 2.5 log10 for Cl. perfringens spores; MS2 and E. coli were rapidly inactivated by > 5 log10. In ODF water, a 4 mg/L dose of MO inactivated ∼3 log10 of C. parvum oocysts and ∼1.5 log10 of Cl. perfringens spores. Inactivation of C. parvum oocysts and Cl. perfringensspores was less extensive at a lower MO dose of 2 mg/L. The use of DAPI and PI to determine viability of oocysts treated with MO did not correlate with, and greatly overestimated, cell culture infectivity. At practical doses and contact times, MO disinfection of water and wastewater achieves appreciable inactivation of both C. parvum oocysts and Cl. perfringens spores. Cl. perfringens spores reliably indicated oocyst inactivation by MO, but E. coli and coliphage MS2 were inactivated much too rapidly to indicate C. parvum inactivation.


2000 ◽  
Vol 41 (7) ◽  
pp. 23-29 ◽  
Author(s):  
T. R. Slifko ◽  
A. Coulliette ◽  
D. E. Huffman ◽  
J. B. Rose

The development of new technologies for Cryptosporidium oocyst detection as well as inactivation and removal is at the forefront of research objectives for the drinking and wastewater industries. One of the major issues associated with testing new technology is determining oocyst viability and infectivity before and after treatment. Because oocysts must be isolated from feces, preparation and pretreatment procedures may affect oocyst infectivity and potentially confound results obtained during survival and disinfection studies. The principal objective of this study was to evaluate the effects of preparation and pretreatment on C. parvum oocyst (Ames, Iowa isolate) infectivity in an in vitro cell culture assay. In vitro excystation, sporozoite yield, and vital dye exclusion using DAPI and PI were used to test viability. A matrix of purification procedures using two defatting agents (ethyl acetate and ethyl ether), cesium chloride (CsCl) and Sheather's solution was evaluated. Effects of immuno-magnetic separation (IMS) and bleach treatment were also assessed. Oocysts purified using CsCl alone showed the most consistent infection from experiment to experiment, compared to preparations that were purified using a defatting agent. Defatting agents, IMS and bleach treatment had no detrimental effects on oocyst infectivity, however, considerable variability between oocyst lots was observed. This study determined that both purification processes and age affect oocyst infectivity.


2002 ◽  
Vol 2 (3) ◽  
pp. 159-168 ◽  
Author(s):  
V. Gitis ◽  
R.C. Haught ◽  
R.M. Clark ◽  
E. Radha Krishnan

Pilot-scale experiments were conducted to investigate removal of Cryptosporidium parvum by contact granular filtration. The research demonstrated enhanced removal of Cryptosporidium parvum in the presence of kaolin particles. This is believed to be due electrostatic adhesion of Cryptosporidium parvum oocysts to the kaolin clay particles. The elementary physico-chemical interactions between filter granules and suspension particles will be discussed. This innovative concept was successfully implemented to reduce the ripening sequence of subsequent filtration experimental test runs by the addition of large surface area particles to slurry of kaolin and Cryptosporidium parvum in surface water.


1997 ◽  
Vol 35 (11-12) ◽  
pp. 261-268 ◽  
Author(s):  
D. C. Johnson ◽  
C. E. Enriquez ◽  
I. L. Pepper ◽  
T. L. Davis ◽  
C. P. Gerba ◽  
...  

Discharge of sewage into the ocean is still a common method of disposal worldwide. Both treated and untreated sewage may contain significant concentrations of waterborne pathogens, such as Giardia, Cryptosporidium, poliovirus and Salmonella. Limited studies exist on the survival of poliovirus and Salmonella in marine waters; however, almost no information exists on the survival of protozoan parasites in marine waters. This study examined the survival of Giardia muris cysts, Cryptosporidium parvum oocysts, poliovirus-1 and Salmonella typhimurium in marine waters. The survival of the microorganisms varied according to the presence of light, salinity and water quality (as determined by quantity of enterococci). All microorganisms survived longer in the dark than in sunlight, the order of survival in sunlight being: Cryptosporidium > poliovirus > Giardia > Salmonella.


2021 ◽  
Vol 7 (2) ◽  
pp. 205521732110227
Author(s):  
Friederike Held ◽  
Sudhakar Reddy Kalluri ◽  
Achim Berthele ◽  
Ana-Katharina Klein ◽  
Markus Reindl ◽  
...  

Background Myelin oligodendrocyte glycoprotein (MOG) antibody disease (MOG-AD) is recognized as a distinct nosological entity. IgG antibodies against MOG (MOG-Ab) overlap with neuromyelitis optica spectrum disorders (NMOSD) phenotype in adults. However, an increasing number of clinical phenotypes have been reported to be associated with MOG-Ab. Objective To investigate the seroprevalence of MOG-Ab under consideration of demographics, disease entities and time course in a large cohort of unselected neurological patients. Methods Blood samples of 2.107 consecutive adult neurologic patients admitted to our department between 2016-2017 were tested for MOG-Ab using a cell-based assay. MOG-Ab persistence was analyzed in follow-up samples. External validation was performed in two independent laboratories. Results We found MOG-Ab in 25 of 2.107 (1.2%) patients. High antibody ratios were mostly associated with NMOSD and MOG-AD phenotype (5/25). Low ratios occurred in a wide range of neurological diseases, predominantly in other demyelinating CNS diseases (5/25) and stroke (6/25). MOG-Ab persistence over time was not confined to NMOSD and MOG-AD phenotype. Conclusion The present study demonstrates the occurrence of MOG-Ab in a wide range of neurological diseases. Only high MOG-Ab ratios were associated with a defined clinical phenotype, but low MOG-Ab ratios were not. The diagnostic value of low MOG-Ab is thus highly limited.


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