Studies of Microthrix parvicella in situ and in laboratory culture: production and use of specific antibodies

2002 ◽  
Vol 46 (1-2) ◽  
pp. 115-118 ◽  
Author(s):  
N. Connery ◽  
A.S. Thompson ◽  
S. Patrick ◽  
M.J. Larkin
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Doubling Time ◽  
Polyclonal Antibodies ◽  
Treatment Plant ◽  
Laboratory Culture ◽  
Specific Antibodies ◽  
Culture Production ◽  
Microthrix Parvicella

Physiological studies on M. parvicella have been conducted to determine the rate of growth of this organism in pure culture. The organism displayed a doubling time of 128 days despite its profuse abundance in a local Wastewater Treatment Plant (WWTW). An extensive survey has been ongoing since February 2000 into the extent of M. parvicella in the WWTW. A suite of monoclonal and polyclonal antibodies has been developed to detect and quantify M. parvicella.

In situ investigations and failure analysis of the rainwater pumps installed in a wastewater treatment plant

2019 ◽  
Vol 109 (S1) ◽  
pp. 42-47
Author(s):  
Sebastian Muntean ◽  
Liviu Marşavina ◽  
Alexandru Hedeş ◽  
Liviu Eugen Anton ◽  
Ilie Vlaicu
Keyword(s):  
Wastewater Treatment ◽  
Failure Analysis ◽  
Wastewater Treatment Plant ◽  
Treatment Plant

Monitoring of a paper mill wastewater treatment plant using UV/VIS spectroscopy

2004 ◽  
Vol 49 (1) ◽  
pp. 9-14 ◽  
Author(s):  
G. Langergraber ◽  
N. Fleischmann ◽  
F. Hofstaedter ◽  
A. Weingartner
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Paper Mill ◽  
Biological Degradation ◽  
Vis Spectroscopy ◽  
Reference Checking ◽  
Paper Mill Wastewater ◽  
Single Instrument

A submersible UV/VIS spectrometer was used to monitor a paper mill wastewater treatment plant. It utilises the UV/VIS range (200-750 nm) for simultaneous measurement of COD, filtered COD, TSS and nitrate with just a single instrument. The instrument measures in-situ, directly in the process. Paper mill wastewater shows typical and reproducible spectra at various process measuring points. There is a relative maximum at 280 mm due to the absorbance by dissolved organic substances, mainly ligninic acids. Comparison of absorbance spectra distinctly shows the decrease of this peak, indicating biological degradation throughout the treatment process. Summarising, one can say that paper mill wastewater cannot be monitored by a simple UV probe measuring only the absorbance at a single wavelength. The required information can only be gained from the whole spectra. Regarding plant control it is suggested that only the overall spectral information is used. Calibrations to conventional parameters are now merely carried out for purposes of reference-checking.

scholarly journals Time-series genome-centric analysis unveils bacterial response to operational disturbance in activated sludge

2019 ◽  
Author(s):  
María Victoria Pérez ◽  
Leandro D. Guerrero ◽  
Esteban Orellana ◽  
Eva L. Figuerola ◽  
Leonardo Erijman
Keyword(s):  
Growth Rate ◽  
Time Series ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Operating Conditions ◽  
The Face

ABSTRACTUnderstanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of three years, including a period of nine month of disturbance, characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons and thein situgrowth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single copy of the ribosomal RNA (rrn) operon. Despite only moderate differences in resource availability, replication rates were distributed uniformly throughout time, with no differences between disturbed and stable periods. We suggest that the length of the growth lag phase, rather than the growth rate, as the primary driver of selection under disturbed conditions. Thus, the system could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions.IMPORTANCEIn this work we investigated the response of microbial communities to disturbances in a full-scale activated sludge wastewater treatment plant over a time-scale that included periods of stability and disturbance. We performed a genome-wide analysis, which allowed us the direct estimation of specific cellular traits, including the rRNA operon copy number and the in situ growth rate of bacteria. This work builds upon recent efforts to incorporate growth efficiency for the understanding of the physiological and ecological processes shaping microbial communities in nature. We found evidence that would suggest that activated sludge could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions. This paper provides relevant insights into wastewater treatment process, and may also reveal a key role for growth traits in the adaptive response of bacteria to unsteady environmental conditions.

Control of the growth of Microthrix parvicella by using an aerobic selector – results of pilot and full scale plant operation

2002 ◽  
Vol 46 (1-2) ◽  
pp. 491-494 ◽  
Author(s):  
M. Lebek ◽  
K.-H. Rosenwinkel
Keyword(s):  
Wastewater Treatment ◽  
Fatty Acid ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Chain Fatty Acid ◽  
Full Scale ◽  
Long Chain Fatty Acid ◽  
Pilot Studies ◽  
Microthrix Parvicella ◽  
Long Chain

A two-stage wastewater treatment plant experiences in bulking sludge problems in winter, correlating with Microthix parvicella abundance. Pilot and full-scale studies of the use of an aerobic selector to control M. parvicella had little success, probably resulting from long chain fatty acid retention in foam at the tank surface. Initial pilot studies with reduced foam retention showed better results.

scholarly journals Time Series Genome-Centric Analysis Unveils Bacterial Response to Operational Disturbance in Activated Sludge

mSystems ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
María Victoria Pérez ◽  
Leandro D. Guerrero ◽  
Esteban Orellana ◽  
Eva L. Figuerola ◽  
Leonardo Erijman
Keyword(s):  
Growth Rate ◽  
Time Series ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Operating Conditions ◽  
Lag Phase

ABSTRACT Understanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of 3 years, including a 9-month period of disturbance characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons, and the in situ growth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single copy of the rRNA (rrn) operon. Despite moderate differences in resource availability, replication rates were distributed uniformly throughout time, with no differences between disturbed and stable periods. We suggest that the length of the growth lag phase, rather than the growth rate, is the primary driver of selection under disturbed conditions. Thus, the system could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions. IMPORTANCE Disturbance is a key determinant of community assembly and dynamics in natural and engineered ecosystems. Microbiome response to disturbance is thought to be influenced by bacterial growth traits and life history strategies. In this time series observational study, the response to disturbance of microbial communities in a full-scale activated sludge wastewater treatment plant was assessed by computing specific cellular traits of genomes retrieved from metagenomes. It was found that the genomes observed in disturbed periods have more copies of the rRNA operon than genomes observed in stable periods, whereas the in situ mean relative growth rates of bacteria present during stable and disturbed periods were indistinguishable. From these intriguing observations, we infer that the length of the lag phase might be a growth trait that affects the microbial response to disturbance. Further exploration of this hypothesis could contribute to better understanding of the adaptive response of microbiomes to unsteady environmental conditions.

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