Distribution of ammonia-oxidizing bacteria in sewage activated sludge: analysis based on 16S rDNA sequence

2004 ◽  
Vol 50 (8) ◽  
pp. 9-14 ◽  
Author(s):  
T. Limpiyakorn ◽  
Y. Shinohara ◽  
F. Kurisu ◽  
O. Yagi
Keyword(s):  
Activated Sludge ◽  
16S Rdna ◽  
Treatment Process ◽  
Sewage Treatment ◽  
Pcr Amplification ◽  
Ammonia Oxidizing Bacteria ◽  
Conventional Activated Sludge ◽  
Process Configuration ◽  
Nitrosomonas Oligotropha ◽  
Activated Sludge Systems

This study carried out analysis of ammonia-oxidizing bacteria (AOB) communities in 12 sewage activated sludge systems standing in eight sewage treatment plants located in Tokyo. The systems were different in the treatment process configuration: anaerobic/anoxic/aerobic (A2O), anaerobic/aerobic (AO), and conventional activated sludge (AS) processes. AOB communities were analyzed by sequences of 16S rDNA amplicons, which were separated by denaturing gradient gel eletrophoresis (DGGE) after specific polymerase chain reaction (PCR) amplification. The results demonstrated that low ammonium concentrations in the influents of the 12 sewage activated sludge systems resulted in the dominance of Nitrosomonas oligotropha-like sequences. Further, Nitrosomonas europaea- and Nitrosomonas cryotolerans-like sequences were recovered from only one A2O system of which the influent contained higher ammonium and chloride concentrations than those of other systems. Nitrosomonas communis-like sequences were found in every A2O and AO system, but mostly not found in every AS system. In summary, influent characteristics and treatment process configuration affected the AOB communities in the 12 sewage activated sludge systems.

High rate and compact single sludge pre-denitrification process for retrofit

1994 ◽  
Vol 30 (6) ◽  
pp. 31-40 ◽  
Author(s):  
Hiroyshi Emori ◽  
Hiroki Nakamura ◽  
Tatsuo Sumino ◽  
Tadashi Takeshima ◽  
Katsuzo Motegi ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Sewage Treatment ◽  
Treatment Plant ◽  
High Rate ◽  
Activated Sludge Process ◽  
Nitrogen And Phosphorus ◽  
Conventional Activated Sludge ◽  
Treatment Techniques ◽  
Conventional Activated Sludge Process ◽  
Denitrification Process

For the sewage treatment plants near rivers and closed water bodies in urbanized areas in Japan and European countries, there is a growing demand for introduction of advanced treatment processes for nitrogen and phosphorus from the viewpoints of water quality conservation and environmental protection. In order to remove nitrogen by the conventional biological treatment techniques, it is necessary to make a substantial expansion of the facility as compared with the conventional activated sludge process. In such urbanized districts, it is difficult to secure a site and much capital is required to expand the existing treatment plant. To solve these problems, a compact single sludge pre-denitrification process using immobilized nitrifiers was developed. Dosing the pellets, which are suitable for nitrifiers growth and physically durable, into the nitrification tank of single sludge pre-denitrification process made it possible to perform simultaneous removal of BOD and nitrogen in a retention time equal to that in the conventional activated sludge process even at the low water temperature of about 10 °C. The 3,000 m3/d full-scale conventional activated sludge plant was retrofitted and has been successfully operated.

scholarly journals Unravelling diversity and metabolic potential of microbial consortia at each stage of leather sewage treatment

RSC Advances ◽  
2017 ◽  
Vol 7 (66) ◽  
pp. 41727-41737 ◽  
Author(s):  
Hebin Liang ◽  
Dongdong Ye ◽  
Lixin Luo
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Treatment Process ◽  
Sewage Treatment ◽  
Microbial Consortia ◽  
Biological Wastewater Treatment ◽  
Metabolic Potential ◽  
Ecological Functions ◽  
Wastewater Treatment Process ◽  
System P

Activated sludge is essential for the biological wastewater treatment process and the identification of active microbes enlarges awareness of their ecological functions in this system.

New approaches to minimize excess sludge in activated sludge systems

2001 ◽  
Vol 44 (10) ◽  
pp. 203-208 ◽  
Author(s):  
G.-H. Chen ◽  
S. Saby ◽  
M. Djafer ◽  
H.-K. Mo
Keyword(s):  
Activated Sludge ◽  
Activated Sludge Process ◽  
Conventional System ◽  
Excess Sludge ◽  
Active Sludge ◽  
New Approaches ◽  
Conventional Activated Sludge ◽  
Sludge Settleability ◽  
Activated Sludge Systems ◽  
Sludge Production

This paper presents three new approaches to reduce excess sludge production in activated sludge systems: 1) modification of conventional activated sludge process with insertion of a sludge holding tank in the sludge return line; 2) chlorination of excess sludge so as to minimize excess sludge production; and 3) utilization of a metabolic uncoupler, 3, 3′, 4′, 5-Tetrachlorosalicylanilide (TCS) to maximize futile activity of sludge microorganisms thereby leading to a reduction of sludge growth. Pilot study was carried out to evaluate this modified activated sludge process (OSA). It has been confirmed that the OSA process is effective in reducing excess sludge; particularly when the ORP level in the sludge holding tank was kept at -250 mV, more than 50% of the excess sludge was reduced. This process can maintain the effluent quality and even perform with a better sludge settleability than a conventional system. Experimental work on the second approach showed that chlorination treatment of excess sludge at a chlorine dose of 0.066 g Cl2/g MLSS reduced the excess sludge by 60%, while concentration of THMS was found below 200 ppb in the treated sludge. However, such sludge chlorination treatment sacrificed sludge settleability. Thus, it is not feasible to introduce the chlorination step to a conventional system. The third approach confirmed that addition of TCS could reduce sludge growth effectively if the TCS concentration is greater than 0.4 ppm. A 0.8-ppm concentration of TCS actually reduced excess sludge by 45%. It was also experimentally demonstrated that presence of TCS increases the portion of active sludge microorganisms over the entire microbial population.

The dynamic behaviour of nitrifying Activated sludge systems influenced by inhibiting wastewater compounds

1995 ◽  
Vol 31 (2) ◽  
pp. 115-124 ◽  
Author(s):  
O. Nowak ◽  
K. Svardal ◽  
P. Schweighofer
Keyword(s):  
Activated Sludge ◽  
Treatment Process ◽  
Dynamic Behaviour ◽  
Competitive Inhibition ◽  
Nitrification Inhibition ◽  
Peak Loads ◽  
Inhibitory Compounds ◽  
Competitive Inhibitors ◽  
Nitrification Process ◽  
Activated Sludge Systems

More or less severe nitrification inhibition was observed in several pilot and full-scale activated sludge plants treating industrial wastewaters. In order to control the treatment process under inhibiting conditions, extended nitrification models have been developed on base of the ‘Activated sludge model No. 1’. In the case of temperatures between 25 and 30°C, the nitrification process has been expressed as a two-step reaction with nitrite as intermediate. Model elements for competitive and non-competitive inhibition as well as for biodegradation of the inhibitor were added, if required. The dynamic behaviour of the investigated activated sludge systems indicates that there are biodegradable non-competitive inhibitors. Operational as well as simulation results show that nitrifying activated sludge plants may become acclimatized to inhibitory compounds but have to be protected from peak loads of both nitrogen and inhibitory compounds.

Molecular Weight Distributions of Soluble Organic Matter in Various Secondary and Tertiary Effluents

1987 ◽  
Vol 19 (3-4) ◽  
pp. 529-538 ◽  
Author(s):  
Gary L. Amy ◽  
Curtis W. Bryant ◽  
Mosen Belyani
Keyword(s):  
Molecular Weight ◽  
Organic Matter ◽  
Activated Sludge ◽  
Water Reuse ◽  
Treatment Process ◽  
Apparent Molecular Weight ◽  
Pure Oxygen ◽  
Soluble Organic Matter ◽  
Conventional Activated Sludge ◽  
Absorbing Material

Differences in the nature of soluble organic matter were measured for various full-scale wastewater treatment processes. Conventional activated sludge, pure oxygen activated sludge, biofiltration, granular activated carbon, and tertiary sand filtration were evaluated. Effluent soluble organic matter was analyzed by ultrafiltration for the apparent molecular weight distribution of soluble organic carbon and UV-absorbing material. The effects of annual season, secondary treatment process, and tertiary treatment process upon the properties of the effluent soluble organic matter were statistically significant at the 99% level. Effluent properties from the various treatments were sufficiently different to support the concept of the selection of appropriate treatments to minimize the effluent concentration of specific fractions of the soluble organic material as required for specific water reuse applications.

Oxygen transfer in activated sludge – new insights and potentials for cost saving

2011 ◽  
Vol 63 (12) ◽  
pp. 3034-3038 ◽  
Author(s):  
J. Henkel ◽  
P. Cornel ◽  
M. Wagner
Keyword(s):  
Activated Sludge ◽  
Nutrient Removal ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Transfer Rate ◽  
Organic Load ◽  
Current Opinion ◽  
Conventional Activated Sludge ◽  
Current State ◽  
Activated Sludge Systems

The α-factor has the greatest impact on the calculation of the required standard oxygen transfer rate (SOTR) in activated sludge systems equipped with submerged aeration systems. Knowing the dependencies of the α-factor leads to a better design of the aeration devices and, consequently, to a more efficient use of aeration energy. Applying the current state of knowledge about oxygen transfer leads to the conclusion that, in contrast to current opinion, simultaneous aerobic stabilization requires the same SOTR as conventional activated sludge systems with advanced nutrient removal, even though a higher organic load is degraded.

Enhancement of 2,4-dichlorophenol degradation in conventional activated sludge systems bioaugmented with mixed special culture

2004 ◽  
Vol 38 (1) ◽  
pp. 245-253 ◽  
Author(s):  
Xiangchun Quan ◽  
Hanchang Shi ◽  
Hong Liu ◽  
Pingping Lv ◽  
Yi Qian
Keyword(s):  
Activated Sludge ◽  
Conventional Activated Sludge ◽  
Activated Sludge Systems

Influence of microbial activity on polar xenobiotic degradation in activated sludge systems

2010 ◽  
Vol 62 (3) ◽  
pp. 701-707 ◽  
Author(s):  
M. Majewsky ◽  
T. Gallé ◽  
L. Zwank ◽  
K. Fischer
Keyword(s):  
Activated Sludge ◽  
Microbial Activity ◽  
Suspended Solids ◽  
Sewage Treatment ◽  
Total Suspended Solids ◽  
Active Biomass ◽  
Sewage Treatment Plants ◽  
Degradation Rates ◽  
Xenobiotic Degradation ◽  
Activated Sludge Systems

The influence of activated sludge quality on the co-metabolic biodegradation of three aminopolycarboxyl acids was investigated for a variety of Luxembourg sewage treatment plants. A combination of biodegradation experiments and respirometric techniques are presented as a reliable approach for the estimation of biokinetics and biological xenobiotic degradation rates that allow for identification of governing parameters such as microbial activity and active biomass. Results showed that biokinetics and degradation rates vary greatly between different plants. The fraction of active biomass on the total suspended solids ranged between 16.9 and 53.7%. Xenobiotic biodegradation rates correlated with microbial activity suggesting a relationship with WWTP performance for carbon and nutrient removal. The biokinetic information can be used to increase the prediction accuracy of xenobiotics removal by individual WWTPs.

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