Short-term effects of carbon source on the competition of polyphosphate accumulating organisms and glycogen accumulating organisms

2004 ◽  
Vol 50 (10) ◽  
pp. 139-144 ◽  
Author(s):  
A. Oehmen ◽  
Z. Yuan ◽  
L.L. Blackall ◽  
J. Keller
Keyword(s):  
Carbon Source ◽  
In Situ Hybridisation ◽  
Operating Conditions ◽  
Fluorescence In Situ Hybridisation ◽  
Biological Phosphorus Removal ◽  
Short Term ◽  
Uptake Rates ◽  
Polyphosphate Accumulating Organisms ◽  
The Impact ◽  
Short Term Effects

The effectiveness of enhanced biological phosphorus removal (EBPR) systems is directly affected by the competition of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs). This study investigated the short-term effects of carbon source on PAO and GAO performance. The tests were designed to clearly determine the impact of volatile fatty acid (VFA) composition on the performance of two types of biomass, one enriched for PAOs and the other for GAOs. The two populations were enriched in separate reactors using identical operating conditions and very similar influent compositions with acetate as the sole carbon source. The only difference was that a very low level of phosphorus was present in the influent to the GAO reactor. The abundance of PAOs and GAOs was quantified using fluorescence in-situ hybridisation. The results clearly show that there are some very distinctive differences between PAOs and GAOs in their ability to utilise different carbon substrates. While both are able to take up acetate rapidly and completely, the GAOs are far slower at consuming propionate than the PAOs during short-term substrate changes. This provides a potentially highly valuable avenue to influence the competition between PAOs and GAOs. Other VFAs studied seem to be less usable in the short term by both PAOs and GAOs, as indicated by their much lower uptake rates.

The effect of GAOs (glycogen accumulating organisms) on anaerobic carbon requirements in full-scale Australian EBPR (enhanced biological phosphorus removal) plants

2003 ◽  
Vol 47 (11) ◽  
pp. 37-43 ◽  
Author(s):  
A.M. Saunders ◽  
A. Oehmen ◽  
L.L. Blackall ◽  
Z. Yuan ◽  
J. Keller
Keyword(s):  
Phosphorus Removal ◽  
In Situ Hybridisation ◽  
Full Scale ◽  
Fluorescence In Situ Hybridisation ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Accumulibacter Phosphatis ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

Glycogen-accumulating organisms (GAOs) were present in six full-scale plants investigated and in all but one made a significant contribution to the amount of volatile fatty acid (VFA) taken up anaerobically. While most plants surveyed contain GAOs, it was demonstrated that it is possible for a full-scale plant to operate with an insignificant GAO population.“Candidatus Accumulibacter phosphatis”were the significant polyphosphate-accumulating organisms (PAOs) in all plants surveyed. “Candidatus Competibacter phosphatis” were found in all plants along with other possible GAOs that were observed but not identified. A significant GAO population will increase the carbon requirements by removing VFA that could otherwise have been used by PAOs. Process optimization minimizing GAOs in full-scale plants would lead to a more efficient use of VFA. Enhanced biological phosphorus removal (EBPR), fluorescence in situ hybridisation (FISH), glycogen accumulating organism (GAO); polyphosphate accumulating organism (PAO);

Denitrification activity of polyphosphate accumulating organisms (PAOs) in full-scale wastewater treatment plants

2018 ◽  
Vol 78 (12) ◽  
pp. 2449-2458 ◽  
Author(s):  
Ana B. Lanham ◽  
Adrian Oehmen ◽  
Gilda Carvalho ◽  
Aaron M. Saunders ◽  
Per H. Nielsen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
In Situ Hybridisation ◽  
Full Scale ◽  
Type I ◽  
Fluorescence In Situ Hybridisation ◽  
Biological Phosphorus Removal ◽  
Aerobic Conditions ◽  
Polyphosphate Accumulating Organisms ◽  
P Removal

Abstract A comprehensive assessment of full-scale enhanced biological phosphorus removal (EBPR) plants (five plants, 19 independent tests) was undertaken to determine their effectiveness in terms of aerobic and anoxic P removal. By comparing parallel P uptake tests under only aerobic or under anoxic-aerobic conditions, results revealed that introducing an anoxic stage led to an overall P removal of on average 90% of the P removed under only aerobic conditions. This was achieved with negligible higher PHA and glycogen requirements, 30% lower overall oxygen consumption and with the simultaneous removal of nitrate, reducing up to an estimate of 70% of carbon requirements for simultaneous N and P removal. Varying fractions of denitrifying polyphosphate accumulating organisms (DPAOs), from an average of 25% to 84%, were found in different plants. No correlation was found between the DPAO fractions and EBPR configuration, season, or the concentration of any of the microbial groups measured via quantitative fluorescence in situ hybridisation. These included Type I and Type II Ca. Accumulibacter and glycogen accumulating organisms, suggesting that chemical batch tests are the best methodology for quantifying the potential of anoxic P removal in full-scale wastewater treatment plants.

Short-Term Culturing Influences the Number of bcr/abl-Fused Cells Detected by Fluorescence in situ Hybridisation in Bone Marrow Aspirates of CML Patients

Pathobiology ◽  
1999 ◽  
Vol 67 (4) ◽  
pp. 163-168 ◽  
Author(s):  
Ludwig Wilkens ◽  
Sabine Varga ◽  
Martin Werner ◽  
Martina Nolte ◽  
Reinhard v. Wasielewski ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridisation ◽  
Fluorescence In Situ Hybridisation ◽  
Short Term

The impact of noise on the short-term effects of traffic-related air pollution on heart rate variability in young healthy adults

2013 ◽  
Vol 2013 (1) ◽  
pp. 4244
Author(s):  
Jing Huang ◽  
Furong Deng ◽  
Shaowei Wu ◽  
Henry Lu ◽  
Yu Hao ◽  
...  
Keyword(s):  
Heart Rate ◽  
Air Pollution ◽  
Heart Rate Variability ◽  
Healthy Adults ◽  
Short Term ◽  
The Impact ◽  
Short Term Effects

Short-Term Impact of Cancer Prevention and Screening Activities on Quality of Life

2004 ◽  
Vol 22 (5) ◽  
pp. 943-952 ◽  
Author(s):  
Jennifer Cullen ◽  
Marc D. Schwartz ◽  
William F. Lawrence ◽  
Joe V. Selby ◽  
Jeanne S. Mandelblatt
Keyword(s):  
Quality Of Life ◽  
Health Status ◽  
Cancer Prevention ◽  
Depression Symptoms ◽  
Original Research ◽  
Short Term ◽  
The Impact ◽  
Short Term Effects

Purpose There are few data on the short-term effects of participating in cancer prevention activities, undergoing genetic risk assessment, or having routine screening. The objective of this article is to systematically review existing research on short-term effects of prevention, genetic counseling and testing, and screening activities on quality of life. Methods We conducted a MEDLINE search for original research studies that were published between January 1, 1985, and December 31, 2002, and conducted in North America or Western Europe. Data were abstracted and summarized using a standardized format. Results We reviewed 210 publications. Most studies focused on psychological states (anxiety, depression), symptoms, or general health status. One hundred thirty-one studies used 51 previously validated noncancer instruments. Many researchers (12.6%) also added cancer-specific measures, such as perceived cancer risk or symptom indices. Only one study measured satisfaction or quality of provider-client communication. While one report examined lost workdays, no other economic consequences of short-term outcomes were evaluated. Among seven studies that assessed short-term outcomes preferences, only four specifically used time trade-off or linear rating scale methods. No study used standard gamble or willingness-to-pay methods. The overwhelming majority of research indicated that short-term effects were transient. Only two studies linked short-term effects to long-term cancer-related health behaviors such as repeat screening. Conclusion There is considerable heterogeneity in short-term outcome measurement. Clinicians need to be aware of potential for short-term, transient adverse effects. The impact of short-term experiences should to be linked to long-term health status and use of services.

Applicability of experience from laboratory reactors with biological phosphorus removal in full-scale plants

2006 ◽  
Vol 54 (1) ◽  
pp. 267-275 ◽  
Author(s):  
E. Tykesson ◽  
L.L. Blackall ◽  
Y. Kong ◽  
P.H. Nielsen ◽  
J. la Cour Jansen
Keyword(s):  
Phosphorus Removal ◽  
Wastewater Treatment Plants ◽  
In Situ Hybridisation ◽  
Full Scale ◽  
Fluorescence In Situ Hybridisation ◽  
Biological Phosphorus Removal ◽  
Batch Tests ◽  
P Release ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) has been used at many wastewater treatment plants all over the world for many years. In this study a full-scale sludge with good EBPR was tested with P-release batch tests and combined FISH/MAR (fluorescence in situ hybridisation and microautoradiography). Proposed models of PAOs and GAOs (polyphosphate- and glycogen-accumulating organisms) and microbial methods suggested from studies of laboratory reactors were found to be applicable also on sludge from full-scale plants. Dependency of pH and the uptake of both acetate and propionate were studied and used for calculations for verifying the models and results from microbial methods. All rates found from the batch tests with acetate were higher than in the batch tests with propionate, which was explained by the finding that only those parts of the bacterial community that were able to take up acetate anaerobically were able to take up propionate anaerobically.

scholarly journals A Glyoxalase-1 Knockdown Does Not Have Major Short Term Effects on Energy Expenditure and Atherosclerosis in Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Markus Wortmann ◽  
Maani Hakimi ◽  
Thomas Fleming ◽  
Andreas S. Peters ◽  
Tjeerd P. Sijmonsma ◽  
...  
Keyword(s):  
Body Weight ◽  
Energy Expenditure ◽  
Advanced Glycation Endproducts ◽  
Standard Diet ◽  
High Fat ◽  
Short Term ◽  
Glyoxalase 1 ◽  
Liver And Kidney ◽  
The Impact ◽  
Short Term Effects

Objective. Glyoxalase-1 is an enzyme detoxifying methylglyoxal (MG). MG is a potent precursor of advanced glycation endproducts which are regarded to be a key player in micro- and macrovascular damage. Yet, the role of Glo1 in atherosclerosis remains unclear. In this study, the effect of Glo1 on mouse metabolism and atherosclerosis is evaluated.Methods. Glo1 knockdown mice were fed a high fat or a standard diet for 10 weeks. Body weight and composition were investigated by Echo MRI. The PhenoMaster system was used to measure the energy expenditure. To evaluate the impact of Glo1 on atherosclerosis, Glo1KDmice were crossed with ApoE-knockout mice and fed a high fat diet for 14 weeks.Results. Glo1 activity was significantly reduced in heart, liver, and kidney lysates derived from Glo1KDmice. Yet, there was no increase in methylglyoxal-derived AGEs in all organs analyzed. The Glo1 knockdown did not affect body weight or body composition. Metabolic studies via indirect calorimetry did not show significant effects on energy expenditure. Glo1KDmice crossed to ApoE−/−mice did not show enhanced formation of atherosclerosis.Conclusion. A Glo1 knockdown does not have major short term effects on the energy expenditure or the formation of atherosclerotic plaques.

scholarly journals Short-term effects of GPS collars on the activity, behavior, and adrenal response of scimitar-horned oryx (Oryx dammah)

2019 ◽  
Author(s):  
Jared A. Stabach ◽  
Stephanie A. Cunningham ◽  
Grant Connette ◽  
Joel L. Mota ◽  
Dolores Reed ◽  
...  
Keyword(s):  
Animal Movement ◽  
Stress Hormones ◽  
Long Term Effects ◽  
Short Term ◽  
High Resolution Data ◽  
Gps Collars ◽  
Behavioral Observations ◽  
Activity Behavior ◽  
The Impact ◽  
Short Term Effects

AbstractGPS collars have revolutionized the field of animal ecology, providing detailed information on animal movement and the habitats necessary for species survival. GPS collars also have the potential to cause adverse effects ranging from mild irritation to severe tissue damage, reduced fitness, and death. The impact of GPS collars on the behavior, stress, or activity, however, have rarely been tested on study species prior to release. The objective of our study was to provide a comprehensive assessment of the short-term effects of GPS collars fitted on scimitar-horned oryx (Oryx dammah), an extinct-in-the-wild antelope once widely distributed across Sahelian grasslands in North Africa. We conducted behavioral observations, assessed fecal glucocorticoid metabolites (FGM), and evaluated high-resolution data from tri-axial accelerometers. Using a series of non-standard regression models, we illustrate clear but short-term effects to animals fitted with GPS collars. Behavioral observations highlighted a significant increase in the amount of headshaking from pre-treatment levels, returning below baseline levels during the post-treatment period (>3 days post-collaring). Similarly, FGM concentrations (i.e., stress hormones) increased after GPS collars were fitted on animals but returned to pre-collaring levels within 5 days of collaring. Lastly, tri-axial accelerometers, collecting data at eight positions per second, indicated a > 480 percent increase in the amount of hourly headshaking immediately after collaring. This post-collaring increase in headshaking was estimated to decline in magnitude within 4 hours after GPS collar fitting. These effects constitute a handling and/or habituation response (model dependent), with animals showing short-term responses in activity, behavior, and stress that dissipated within several hours to several days of being fitted with GPS collars. Importantly, none of our analyses indicated any long-term effects that would have more pressing animal welfare concerns.

scholarly journals The Short-Term Effects of European Integration on Mortality Convergence: A Case Study of European Union’s 2004 Enlargement

2021 ◽  
Author(s):  
Rok Hrzic ◽  
Tobias Vogt ◽  
Helmut Brand ◽  
Fanny Janssen
Keyword(s):  
European Integration ◽  
National Level ◽  
The European Union ◽  
Eu Accession ◽  
Short Term ◽  
Member States ◽  
Sigma Convergence ◽  
The Impact ◽  
Short Term Effects

AbstractAlthough European integration can be expected to result in mortality convergence (reduced mortality differences), a life expectancy divide persists in the European Union (EU) between the old Member States (OMS) in the west and the new Member States (NMS) in the east. Studies investigating the impact of European integration on mortality convergence are rare and did not consider regional differences. We examine the short-term effects of the 2004 enlargement on mortality convergence at the supranational, national, and subnational levels. Using sex-specific life expectancies for 23 Member States (1990–2017) and the NUTS 2 regions in Czechia, Hungary, and Poland for 1992–2016, we examined the trend in sigma and beta mortality convergence measures at the country and regional levels using joinpoint regression. We found no compelling evidence that EU accession influenced the process of mortality convergence between OMS and NMS, or within the three NMS, over the short term. While there was overall beta and sigma convergence at the national level during 1990–2017, no regional convergence showed, and the trends in convergence did not significantly change at the time of EU accession or soon after (2004–2007). The accession in 2004 did not visibly impact the overall process of mortality convergence over the short term, likely because of the greater influence of country and region-specific policies and characteristics. The interaction of Member State and regional contexts with the mechanisms of European integration requires further study. Future enlargement procedures should emphasise tailored support to ensure more equitable gains from European integration.

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