Hydrolysis and fermentation of activated sludge to enhance biological phosphorus removal

2006 ◽  
Vol 53 (12) ◽  
pp. 55-64 ◽  
Author(s):  
J. Vollertsen ◽  
G. Petersen ◽  
V.R. Borregaard

The conventional mainstream enhanced biological phosphorus removal (EBPR) process depends on the quality of the raw incoming wastewater. An alternative sidestream EBPR process is presented, where the substrates for storage by the polyphosphate accumulating organisms (PAOs) instead come from hydrolysis of the return activated sludge. This process is studied in full-scale at two treatment plants and quantified by means of phosphorus release rates and readily biodegradable COD (RBCOD) accumulation rates. It was seen that not only was a significant amount of RBCOD stored by PAOs but an approximately equal amount was accumulated in the sidestream hydrolysis tank and made available for the subsequent nitrogen removal process. The phosphorus release of the sludge with and without addition of different substrates was furthermore studied in laboratory scale. The study showed that the process is promising and in a number of cases will have significant advantages compared with the conventional mainstream EBPR design.

1986 ◽  
Vol 13 (3) ◽  
pp. 345-351 ◽  
Author(s):  
B. Rabinowitz ◽  
W. K. Oldham

This paper examines the role of short-chain volatile fatty acids in the excess biological phosphorus removal mechanism of the activated sludge process. The effectiveness of various substrate additions in inducing phosphorus removal was investigated through a series of laboratory and pilot-scale experiments. Phosphorus release and substrate uptake both take place in the anaerobic zone of the process and there appears to be an exchange phenomenon that occurs between the two molecules. The system phosphorus removal of the process is improved by the addition of sodium acetate to the anaerobic zone. It is important that the zone receives no incoming nitrate, as the added substrate will be oxidized in the denitrification reaction, rendering it unavailable for the phosphorus removal mechanism. Acetate and propionate, the two substrates that are most effective in inducing anaerobic phosphorus release, can be generated on-site at a treatment plant by primary sludge fermentation in concentrations sufficient to significantly enhance the phosphorus removal characteristics of the process. Key words: biological phosphorus removal, short-chain volatile fatty acids, phosphorus release, substrate utilization, primary sludge fermentation.


2015 ◽  
Vol 8 ◽  
pp. ASWR.S27218 ◽  
Author(s):  
Qiuyan Yuan ◽  
Richard Sparling ◽  
Jan Oleszkiewicz

In this study, a sequencing batch reactor was set up and operated for over three months to cultivate polyphosphate-accumulating organisms polyphosphate-accumulating organisms (PAOs) in the enriched activated sludge. Batch studies were then carried out to study the effect of different carbon sources on phosphorus removal as well as polyhydroxybutyrate (PHB) production. The carbon sources investigated were acetate, glucose, wastewater, and beef extract. It was found that enhanced biological phosphorus removal could not be achieved using glucose as substrate. This suggested that glucose was not a good candidate for biological phosphorus removal. In terms of PHB production, using acetate and glucose as substrate resulted in PHB production of 42% and 40%, respectively, of the dry cell weight (DCW). Lower PHB production was obtained from using municipal wastewater and beef extract as a carbon source. This resulted in ∼15% and 13% of DCW. It was concluded that municipal wastewater activated sludge can be an economic alternative for PHB production if municipal wastewater is mixed with certain kinds of carbon-enriched industrial wastewater.


1985 ◽  
Vol 17 (11-12) ◽  
pp. 297-298 ◽  
Author(s):  
Takao Murakami ◽  
Atsushi Miyairi ◽  
Kazuhiro Tanaka

In Japan various biological phosphorus removal processes have recently been researched by laboratory or pilot plant scale studies and most of them have shown good results. Based on these results, the Japan Sewage Works Agency has conducted a full scale study of the biological phosphorus removal process from June 1982 until February 1983, which was the first full scale operation of this process in Japan. The main purpose of the study was to evaluate phosphorus removal efficiency and also nitrogen removal efficiency of the process and in addition, to ascertain the important operating factors of the process. For the study a treatment train of a large scale sewage treatment plant was remodelled. The aeration tank of 3.825 m3 volume was divided into four equal cells. The whole train including return sludge line was operated entirely independently of the other trains. During the experiment the train was operated under two different modes, Mode 1 and Mode 2. In Mode 1, the train was operated as an A/O process, the first cell of the aeration tank being anaerobic and the other cells oxic. In Mode 2, the train was operated as a Modified Phoredox process. In this case, the first cell was anaerobic, but the second cell was anoxic and nitrified liquor was returned to it from the end of the oxic cells. Mode 1 and Mode 2 were further divided into many ‘runs' and the flow rate varied between 12,550 m3 d−1 and 25,270 m3 d−1 , corresponding to retention times of 7.3 hours and 3.6 hours, respectively. Throughout the experimental period the mean value of influent (primary effluent) total-P concentration was 3.38 mg 1−1 , and that of the final effluent was 0.47 mg 1−1 . A cumulated frequency curve of the data showed that about 93% of measured effluent total-P was below 1.0 mg l−1 . Therefore, it can be concluded that with these influent total-P levels, biological phosphorus removal processes can sufficiently satisfy the effluent standard of 1 mg 1−1 total-P. Even when the process was operated as a Modified Phoredox Process, no obstruction to phosphorus removal because of nitrification was observed and phosphorus removal remained good. However, since the sewage treatment plant treated influent from a combined sewerage system, phosphorus removal was sometimes affected by heavy rainfalls. In such cases phosphorus release in the anaerobic cell was insufficient because of increased influent NOx concentration and accordingly increased denitrification level in the anaerobic cell. Therefore, as a result, enhanced phosphorus uptake in the following cells could not be observed. Higher process stability can be expected if an effective countermeasure to high influent NOx concentration can be made. Influence of flow rate fluctuation on the process was also studied. The treatment train was operated for a week under a daily flow rate fluctuation pattern which ranged between 460 m3 hr−1 and 820 m3 hr−1 . Nevertheless, the effluent total-P concentration showed no increase and stayed constantly lower than 0.5 mg 1−1. The oxidation reduction potential (ORP) was an effective control index to evaluate the degree of phosphorus release in the anaerobic cell. Water temperature did not affect phosphorus release and uptake rates.


1999 ◽  
Vol 39 (6) ◽  
pp. 37-43 ◽  
Author(s):  
Damir Brdjanovic ◽  
Mark C. M. van Loosdrecht ◽  
Christine M. Hooijmans ◽  
Takashi Mino ◽  
Guy J. Alaerts ◽  
...  

This paper describes several techniques and methods for sludge characterization which are recently developed and used in our ongoing research on biological phosphorus removal (BPR): (i) chemical measurement of the poly-hydroxy-alkanoate (PHA) content of the biomass, (ii) bioassay for glycogen determination in BPR systems, (iii) determination of the fraction of denitrifying phosphorus accumulating organisms (DPAOs) in P-removing sludge, (iv) determination of the aerobic and anoxic ATP/NADH2 ratio (δ value) in BPR systems, and (v) determination of the glycogen accumulating organisms (GAOs) / phosphorus accumulating organisms (PAOs) ratio in activated sludge.


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