Application of heat shock protein assay and proteome assay to water from wastewater treatment plant

2008 ◽  
Vol 57 (8) ◽  
pp. 1183-1189 ◽  
Author(s):  
Naoyuki Funamizu ◽  
Mikako Takenaka ◽  
Junkyu Han ◽  
Hiroko Isoda
Keyword(s):  
Wastewater Treatment ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cho Cells ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Chinese Hamster ◽  
Decay Process ◽  
Aeration Tank ◽  
Sludge Treatment

In this study we applied bioassay using Chinese hamster ovary (CHO) cells with a heat shock protein (HSP) 47 promoter to the effluent of the wastewater treatment plants in Sapporo and we observed the statistically significant HSP production. This implied the effluent contained some organic matter which can stress the CHO cells. To investigate the possible causes of the toxicity of the effluent, we applied the assay to the rejected water from the sludge treatment plant, the mixtures of sewage and rejected water. The evolution of HSP production during the aerobic decay process and thickening process of sludge was also examined. These assay results showed that dissolved microbial products generated and/or released from activated sludge during its decay process in the aeration tank and during thickening and dewatering process in the sludge treatment train contributed to develop HSP production. The proteomics analysis was also applied to the effluent and detected the production of elongation factor 1β. This result implies that the effluent from wastewater treatment plants may cause changes in cell proteins involved in allergic reaction.

Identification of a protein altered in mutants resistant to microtubule inhibitors as a member of the major heat shock protein (hsp70) family

1990 ◽  
Vol 10 (10) ◽  
pp. 5160-5165
Author(s):  
S Ahmad ◽  
R Ahuja ◽  
T J Venner ◽  
R S Gupta
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Immunofluorescent Staining ◽  
Mutant Form ◽  
Two Dimensional ◽  
Wild Type ◽  
Cho Cell ◽  
Microtubule Inhibitors

A major cellular protein (P2; approximately 70 kilodaltons) which is altered in Chinese hamster ovary (CHO) cell mutants resistant to the microtubule inhibitors colchicine and podophyllotoxin has been shown to correspond to the constitutive form of the 70-kilodalton heat shock protein (hsc70). The inference that P2 and hsc70 are the same protein is based on the following observations: (i) migration of P2 in two-dimensional polyacrylamide gels in the same position as that reported for hsc70; (ii) cross-reactivity of a monoclonal antibody which reacts with both the constitutive and induced forms of hsp70 with the P2 spot from wild-type CHO cells and with both P2 and a mutant form of P2 in a CHO cell mutant; (iii) specific reactivity of a polyclonal antibody to P2 with both the constitutive and heat-induced forms of hsp70 in human cells; (iv) identical immunofluorescent staining of dot/patchlike structures with both P2 and hsp70 antibodies in human and CHO cells; and (v) a cDNA clone for hsc70 has been isolated and sequenced from wild-type CHO cells. The in vitro transcription and translation product of this cDNA has been shown to comigrate with the P2 protein spot in two-dimensional gels, indicating their identity. The fact that there is an alteration in hsc70 in mutants resistant to antimitotic drugs suggests a role for this protein in the in vivo assembly and function of microtubules.

scholarly journals Identification of a protein altered in mutants resistant to microtubule inhibitors as a member of the major heat shock protein (hsp70) family.

1990 ◽  
Vol 10 (10) ◽  
pp. 5160-5165 ◽  
Author(s):  
S Ahmad ◽  
R Ahuja ◽  
T J Venner ◽  
R S Gupta
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Immunofluorescent Staining ◽  
Mutant Form ◽  
Two Dimensional ◽  
Wild Type ◽  
Cho Cell ◽  
Microtubule Inhibitors

A major cellular protein (P2; approximately 70 kilodaltons) which is altered in Chinese hamster ovary (CHO) cell mutants resistant to the microtubule inhibitors colchicine and podophyllotoxin has been shown to correspond to the constitutive form of the 70-kilodalton heat shock protein (hsc70). The inference that P2 and hsc70 are the same protein is based on the following observations: (i) migration of P2 in two-dimensional polyacrylamide gels in the same position as that reported for hsc70; (ii) cross-reactivity of a monoclonal antibody which reacts with both the constitutive and induced forms of hsp70 with the P2 spot from wild-type CHO cells and with both P2 and a mutant form of P2 in a CHO cell mutant; (iii) specific reactivity of a polyclonal antibody to P2 with both the constitutive and heat-induced forms of hsp70 in human cells; (iv) identical immunofluorescent staining of dot/patchlike structures with both P2 and hsp70 antibodies in human and CHO cells; and (v) a cDNA clone for hsc70 has been isolated and sequenced from wild-type CHO cells. The in vitro transcription and translation product of this cDNA has been shown to comigrate with the P2 protein spot in two-dimensional gels, indicating their identity. The fact that there is an alteration in hsc70 in mutants resistant to antimitotic drugs suggests a role for this protein in the in vivo assembly and function of microtubules.

scholarly journals Assessment of the current status of waste sludge treatment of the industrial parks in Binh Duong Province

2020 ◽  
pp. 435-441
Keyword(s):  
Wastewater Treatment ◽  
Industrial Waste ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Current Status ◽  
Industrial Park ◽  
Sludge Treatment ◽  
Waste Sludge ◽  
Helminth Eggs ◽  
Industrial Parks

Study on sampling sludge from 04 industrial parks in Binh Duong province such as My Phuoc, Dong An, Viet Huong 1, Song Than 2 to analyze the pollution criteria to assess the pollution possibility of industrial waste sludge. The research results show that: at present, the total volume of sludge generated today in the industrial park fluctuates about 4,450 – 6,255 kg/day, equivalent to dry sludge volume is 1,035.725 – 1,532.11 kg/day. The estimated volume of dry sludge generated from industrial parks in the future from 2019 to 2020 ranges from 30,023 to 31,396 kg/day. Most sludge from 04 wastewater treatment plants hadn’t heavy metals (Hg, Pb, Cu, Ni, Cr, Zn, Cd), especially Dong An Industrial park Treatment plant had Zn, value 49.7 – 49.9 mg/l. The sludge from the wastewater treatment plants of 04 industrial parks had amount of helminth eggs that exceeds the USEPA's regulations on pathogens (type A sludge).

scholarly journals Effect of Tank Shape on Performance of Aeration Tank in Sewage Treatment

2019 ◽  
Vol 9 (2) ◽  
pp. 475-480
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Sewage Treatment ◽  
Settling Tank ◽  
Treatment Plant ◽  
Pilot Scale ◽  
Aeration Tank ◽  
Effective Parameters ◽  
Operation Costs ◽  
Different Depths

This Study Was Made To Study The Use Of Circular Aeration Tanks Instead Of Rectangular One In Wastewater Treatment Plants. The Study Covered The Effect Of Tank Geometric Shape On Action Stability, Effective Parameters Homogeneity And Treatment Efficiency Inside The Aeration Tank And Its Reflection On The Final Sedimentation Tank Performance. A Pilot Scale Was Erected In Balaqs Wastewater Treatment Plant Pilot Consists From Two Lines One Circular Aeration Tank Followed By Final Settling Tank And Second Rectangular Aeration Tank Followed By Final Settling Tank For Comparison Purpose Under The Same Conditions. The Samples Were Taken Continuously For 5 Weeks From The Inlet, Outlet For (Bod, & Tss) To Measure The Aeration Removal Efficiency. Also Measurements Inside Both Types Of Aeration Tanks To Determine The Parameters Of Temperature & Do Distribution And Stability In Different Depths And Sides Of Tank. Also The Consumed Power Had Been Measured. The Results Shows That The Circular Aeration Tank Achieved Better Stability Inside The Tank With Minimal Variation In Both Of Do And Temperature That Varied Widely In The Rectangular Tank Between Different Depths And Also Longitudinally And In Cross Section Directions That Affects Mainly On The Tank Efficiency And The Consumed Power Needed For Surface Aerators Operation. The Circular Safe About 50% Of The Consumed Power That Also Safe In The Construction And Operation Costs For Such Treatment.

Description and test of a new generation of nutrient sensors

1996 ◽  
Vol 33 (1) ◽  
pp. 25-35 ◽  
Author(s):  
A. Lynggaard-Jensen ◽  
N. H. Eisum ◽  
I. Rasmussen ◽  
H. Svankjær Jacobsen ◽  
T. Stenstrøm
Keyword(s):  
Wastewater Treatment ◽  
Response Times ◽  
Wastewater Treatment Plants ◽  
Waste Water Treatment ◽  
Flow Analysis ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Pilot Scale ◽  
Waste Water Treatment Plant ◽  
Aeration Tank

Sensor prototypes for measurement of ammonium, nitrate and phosphate in wastewater treatment plants are described together with the results obtained in laboratory and pilot scale wastewater treatment plants. A functional description of the sensor principles is presented together with the installation and operation procedures. Basically the measurements are done using membrane technology in combination with semi-micro Continuous Flow Analysis (μCFA) with classic colorimetry. Because of this the sensors can be installed directly in the aeration tanks without any need for sampling, filtration, etc. Furthermore, the semi-micro scale is used in such a way, that handling of chemicals and waste is a closed loop in a package to be changed once a month. The sensors have been tested thoroughly in a pilot scale waste water treatment plant (recirculation) using real raw wastewater as well as artificial wastewater. The sensors have been placed directly in the aeration tank or in the anoxic tank of the pilot plant. The tests show very little, if any, fouling problems due to the membrane material used. The test results show a good reproducibility and most important, compared to other available sensors/analyzers on the market, very low response times, less than 5 minutes. Owing to these low response times, experiments with direct measurement of nitrification and denitrification rates were carried out.

Environmental Impact of the Microbial Aerosol Emissions from Wastewater Treatment Plants

1982 ◽  
Vol 14 (1-2) ◽  
pp. 289-309 ◽  
Author(s):  
C Lue-Hing ◽  
D R Zenz ◽  
S J Sedita
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Ambient Air ◽  
Waste Water Treatment Plant ◽  
Aeration Tank ◽  
Major Study ◽  
Aerosol Emissions

The literature on microbial emissions from wastewater treatment plants is reviewed and a major study of microbial aerosol emissions from such a plant is described and discussed. The literature was found to be repleted with studies which showed that microbial aerosols are emitted from wastewater treatment plants but rapid die-away was shown once the aerosols reach the ambient air. Epidemiological studies of wastewater treatment plant workers and of communities nearby such plants gave overwhelming evidence to show that wastewater treatment plants do not affect the health of the local populace nor the plant workers themselves. The paper describes a major study of the microbial aerosol emissions of a waste-water treatment plant operated by the Metropolitan Sanitary District of Greater Chicago. In this study, it was found that aerosols containing microorganisms were emitted from the aeration tank surface at rates of 208 to 386 standard plate counts per cubic meter of air at a distance of 0.3 m above the surface. However, it was found that there was rapid die-away of microbial emission with distance from the aeration tanks both horizontally and vertically. It was concluded, based upon the literature review and the major study described, that microbial aerosol emissions from wastewater treatment plants were not a significant factor in the health of the surrounding populace.

scholarly journals Particle balance and return loops for microplastics in a tertiary-level wastewater treatment plant

2021 ◽  
Author(s):  
Pauliina Salmi ◽  
Kalle Ryymin ◽  
Anna K. Karjalainen ◽  
Anna Mikola ◽  
Emilia Uurasjärvi ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Wastewater Sludge ◽  
Tertiary Level ◽  
Sludge Treatment ◽  
Reject Water ◽  
Exit Route ◽  
Sampling Campaign ◽  
The One

Abstract Microplastics (MPs) from households, stormwater, and various industries are transported to wastewater treatment plants (WWTPs), where a high proportion of them are captured before discharging their residuals to watersheds. Although recent studies have indicated that the removed MPs are mainly retained in wastewater sludge, sludge treatment processes have gained less attention in MP research than water streams at primary, secondary, and tertiary treatments. In this study, we sampled twelve different process steps in a tertiary-level municipal WWTP in central Finland. Our results showed that, compared to the plant influent load, three times more MPs circulated via reject water from the sludge centrifugation back to the beginning of the treatment process. Especially fibrous MPs were abundant in the dewatered sludge, whereas fragment-like MPs were observed in an aqueous stream. We concluded that, compared to the tertiary effluent, sludge treatment is the major exit route for MPs into the environment, but sludge treatment is also a return loop to the beginning of the process. Our sampling campaign also demonstrated that WWTPs with varying hydraulic conditions (such as the one studied here) benefit from disc filter–based tertiary treatments in MP removal.

scholarly journals Methodology for Optimizing Sewage Sludge Treatment, Valorization and Disposal Methods. Application to the Marrakech (Morocco) Wastewater Treatment Plant

2020 ◽  
Vol 9 (4) ◽  
pp. 305-318
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Mathematical Formulation ◽  
Operating Cost ◽  
Treatment Plant ◽  
Optimal Solution ◽  
Sludge Management ◽  
Optimal Method ◽  
Sludge Treatment ◽  
Support Tool

Wastewater treatment leads to a significant production of about 150 to 200 kg of sludge per population equivalent (P.E) per year, with a humidity level higher than 90%. The treatment and discharge of this sludge involves high costs for wastewater treatment plants (WWTPs), which can amount to up to 60% of their total operating cost. The environmental and public health aspect should also be carefully considered during evacuation as sludge may contain harmful elements. Currently, several treatment, valorization and disposal methods are possible for sludge. In order to respect the various constraints on the possibilities of discharging sludge and to minimize its cost, a reasonable approach must be followed to establish an optimal management path according to the WWTP situation. The objective of this work is to develop a methodology for optimizing sludge treatment, valorization and disposal methods for a given WWTP. This method is based on operational research technique. The methodology follows the following six steps: description of the existing situation, identification of possible treatment, valorization and disposal methods for the WWTP and development of a decision tree specific to the WWTP, mathematical formulation and assumptions, mathematical resolution and sensitivity analysis, validation of the solution and application of the solution. After the application of this methodology, a decision support tool is developed to define the optimal method(s) for sludge management in the WWTP. According to the example of the application of this methodology on sludge management of the WWTP of Marrakech (Morocco), the optimal solution, with respect to the data used and keeping the installations in place, is the application of agricultural spreading after the sludge treatments which are: thickening, anaerobic digestion, mechanical dehydration and solar drying. On a 20-year operation, an average gain of about €10 (110 DHs) per ton of dry solid (DS) incoming is generated.

scholarly journals THE INFLUENCE OF AERATOR MOUNTING AND WASTEWATER TREATMENT PLANT DESIGN ON THE PERFORMANCE OF AERATION SYSTEMS

2010 ◽  
Vol 2 (5) ◽  
pp. 97-102
Author(s):  
Ala Sokolova ◽  
Mindaugas Rimeika
Keyword(s):  
Wastewater Treatment ◽  
Oxygen Transfer ◽  
Wastewater Treatment Plants ◽  
Oxygen Transfer Rate ◽  
Treatment Plant ◽  
Aeration Tank ◽  
Plant Design ◽  
Operational Parameters ◽  
Aeration System ◽  
The Impact

The paper analyzes the impact of the way of mounting a tube diffuser, the design of an aeration tank and the presence of a fixed carrier on the operational parameters of aeration systems used in small wastewater treatment plants. It was found out that the vertically mounted tube diffuser decreased standard oxygen transfer rate (SOTR) of the aeration system by approximately 20% and standard oxygen transfer efficiency (SOTE) by 25% comparing to the horizontally mounted tube diffuser. It was also defined that the design of the aeration tank might have an impact on the operation parameters of the aeration system: when the centre shell used to protect a diffuser was dismantled from a test tank, SOTR and SOTE increased by approximately 20%. It was also established that the presence of the fixed carrier in the aeration tank did not have an impact on the performance of aeration systems. Finally, research was carried out to compare the operational parameters of two diffusers of different types offered on the market and used in small wastewater treatment plants. It was found out that the performance different type diffusers might vary considerably.

scholarly journals "Aeration Tank Behavior in the Activated Sludge Wastewater Treatment Plant Startup Conditions Case study; (Wastewater Treatment plant of General Mosul hospital- IRAQ)"

2013 ◽  
Vol 1 (1) ◽  
pp. 1-13
Author(s):  
Riyadh M. S. Al-Obaidi
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Organic Content ◽  
Aeration Tank ◽  
Surface Aeration ◽  
Start Up ◽  
Simple Growth

The biological unit in the wastewater treatment plants can be considered the most sensitive treatment units. It begins work with dynamic conditions for several weeks until reach the steady state conditions. Therefore, this study was done to observe aeration tank behavior (activated sludge unit) in the wastewater treatment plant of general hospital complex of Mosul city. Sampling made daily for more than 30 days. The biological treatment monitoring parameter was tested. The study shown that there is need to about 45-60 days to complete the start up work to be the operation stable and successful (if it worked without seeding). There was simple growth of microorganisms with modest treatment of organic matter, and then it rose after 3 first weeks of operation. There was contrary relationship between organic content and suspended solids in the aeration tank; an equation of this relation has been presented. The pH has risen in the first days in the effluent wastewater, then return to be less than influent pH with a relative relation with the activity of the microorganisms and surface aeration in the tank. The EC was simply reduced in the Effluent along with the study.

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