scholarly journals Polyhydroxyalkanoate (PHA) production from sludge and municipal wastewater treatment

2013 ◽  
Vol 69 (1) ◽  
pp. 177-184 ◽  
Author(s):  
F. Morgan-Sagastume ◽  
F. Valentino ◽  
M. Hjort ◽  
D. Cirne ◽  
L. Karabegovic ◽  
...  

Polyhydroxyalkanoates (PHAs) are biodegradable polyesters with comparable properties to some petroleum-based polyolefins. PHA production can be achieved in open, mixed microbial cultures and thereby coupled to wastewater and solid residual treatment. In this context, waste organic matter is utilised as a carbon source in activated sludge biological treatment for biopolymer synthesis. Within the EU project Routes, the feasibility of PHA production has been evaluated in processes for sludge treatment and volatile fatty acid (VFA) production and municipal wastewater treatment. This PHA production process is being investigated in four units: (i) wastewater treatment with enrichment and production of a functional biomass sustaining PHA storage capacity, (ii) acidogenic fermentation of sludge for VFA production, (iii) PHA accumulation from VFA-rich streams, and (iv) PHA recovery and characterisation. Laboratory- and pilot-scale studies demonstrated the feasibility of municipal wastewater and solid waste treatment alongside production of PHA-rich biomass. The PHA storage capacity of biomass selected under feast–famine with municipal wastewater has been increased up to 34% (g PHA g VSS−1) in batch accumulations with acetate during 20 h. VFAs obtained from waste activated sludge fermentation were found to be a suitable feedstock for PHA production.

2013 ◽  
Vol 20 (1) ◽  
pp. 199-208 ◽  
Author(s):  
Marketa Julinova ◽  
Jan Kupec ◽  
Roman Slavik ◽  
Maria Vaskova

Abstract A synthetic polymer, polyvinylpyrrolidone (PVP - E 1201) primarily finds applications in the pharmaceutical and food industries due to its resistance and zero toxicity to organisms. After ingestion, the substance passes through the organism unchanged. Consequently, it enters the systems of municipal wastewater treatment plants (WWTP) without decomposing biologically during the waste treatment process, nor does it attach (through sorption) to particles of activated sludge to any significant extent, therefore, it passes through the system of a WWTP, which may cause the substance to accumulate in the natural environment. For this reason the paper investigates the potential to initiate aerobic biodegradation of PVP in the presence of activated sludge from a municipal wastewater treatment plant. The following agents were selected as the initiators of the biodegradation process - co-substrates: acrylamide, N-acethylphenylalanine and 1-methyl-2-pyrrolidone, a substance with a similar structure to PVP monomer. The biodegradability of PVP in the presence of co-substrates was evaluated on the basis of biological oxygen demand (BOD) as determined via a MicroOxymax O2/CO2/CH4 respirometer. The total substrate concentration in the suspension equaled 400 mg·dm-3, with the ratio between PVP and the cosubstrate being 1:1, while the concentration of the dry activated sludge was 500 mg·dm-3. Even though there was no occurrence of a significant increase in the biodegradation of PVP alone in the presence of a co-substrate, acrylamide appeared to be the most effective type of co-substrate. Nevertheless, a recorded decrease in the slope of biodegradation curves over time may indicate that a process of primary decomposition was underway, which involves the production of metabolites that inhibit activated sludge microorganisms. The resulting products are not identified at this stage of experimentation.


2020 ◽  
Vol 12 (11) ◽  
pp. 4758
Author(s):  
Huyen T.T. Dang ◽  
Cuong V. Dinh ◽  
Khai M. Nguyen ◽  
Nga T.H. Tran ◽  
Thuy T. Pham ◽  
...  

Fixed-film biofilm reactors are considered one of the most effective wastewater treatment processes, however, the cost of their plastic bio-carriers makes them less attractive for application in developing countries. This study evaluated loofah sponges, an eco-friendly renewable agricultural product, as bio-carriers in a pilot-scale integrated fixed-film activated sludge (IFAS) system for the treatment of municipal wastewater. Tests showed that pristine loofah sponges disintegrated within two weeks resulting in a decrease in the treatment efficiencies. Accordingly, loofah sponges were modified by coating them with CaCO3 and polymer. IFAS pilot tests using the modified loofah sponges achieved 83% organic removal and 71% total nitrogen removal and met Vietnam’s wastewater effluent discharge standards. The system achieved considerably high levels of nitrification and it was not limited by the loading rate or dissolved oxygen levels. Cell concentrations in the carriers were twenty to forty times higher than those within the aeration tank. Through 16S-rRNA sequencing, the major micro-organism types identified were Kluyvera cryocrescens, Exiguobacterium indicum, Bacillus tropicus, Aeromonas hydrophila, Enterobacter cloacae, and Pseudomonas turukhanskensis. This study demonstrated that although modified loofah sponges are effective renewable bio-carriers for municipal wastewater treatment, longer-term testing is recommended.


1991 ◽  
Vol 23 (4-6) ◽  
pp. 1097-1106 ◽  
Author(s):  
H. Nakazawa ◽  
K. Tanaka

Mathematical models based on the kinetic aspect of the sequencing batch activated sludge process were developed to explain the characteristics of the process treating municipal wastewater. These models are a steady-state model dealing with the overall relationship between biomass concentrations in a reactor and operational conditions of the process, and a kinetic model dealing with the behaviors of biomass and substrate in a reactor within one cycle time of the process. Applying these mathematical models for the results of pilot-scale experiments for municipal wastewater treatment, reasonable parameters' values were obtained and the effects of operating strategies including the aeration time ratio and the solids retention time became clear for the process performance.


1996 ◽  
Vol 33 (12) ◽  
pp. 199-210
Author(s):  
R. Gnirss ◽  
A. Peter-Fröhlich ◽  
V. Schmidt

For municipal wastewater treatment, space-saving 10m deep activated sludge tanks are an interesting alternative to conventional tanks of shallow construction. Results from pilot tests in the Berlin-Ruhleben WWTP have shown that the biological P-elimination, nutrification and denitrification processes can be implemented as in shallow tanks. However, the activated sludge did not settle satisfactorily. Flotation was implemented in the process for secondary clarification and in the meanwhile has shown to be advantageous. Tests run over a period of some years with the pilot plant have proven the feasibility of this process. Energy requirements for both systems were found to be approximately the same. A cost estimate based on a preplan revealed a 10% advantage in favour of the 10m WWTP with flotation. For these reasons, one 10m WWTP with flotation for secondary clarification and a capacity of 80,000 m3/d will be built in Berlin in the near future.


1994 ◽  
Vol 30 (4) ◽  
pp. 211-214 ◽  
Author(s):  
E. Brands ◽  
M. Liebeskind ◽  
M. Dohmann

This study shows a comparison of important parameters for dynamic simulation concerning the highrate and low-rate activated sludge tanks of several municipal wastewater treatment plants. The parameters for the dynamic simulation of the single-stage process are quite well known, but parameters for the high-ratellow-rate activated sludge process are still missi ng, although a considerable number of wastewater treatment plants are designed and operated that way. At present any attempt to simulate their operation is restricted to the second stage due to missing data concerning growth rate, decay rate, yield coefficient and others.


2003 ◽  
Vol 48 (5) ◽  
pp. 257-266 ◽  
Author(s):  
K. Boonsong ◽  
S. Piyatiratitivorakul ◽  
P. Patanaponpaiboon

The study evaluated the possibility of using mangrove plantation to treat municipal wastewater. Two types of pilot scale (100 × 150 m2) free water surface constructed wetland were set up. One system was a natural Avicennia marina dominated forest system. The other system was a newly planted system in which seedlings of Rhizophora spp., A. marina, Bruguiera cylindrica and Ceriops tagal were planted in 4 strips. Municipal wastewater was retained within the systems for 7 and 3 days, respectively. The results indicated that the average removal percentage of TSS, BOD, NO3-N, NH4-N, TN, PO4-P and TP in the newly planted system were 27.6-77.1, 43.9-53.9, 37.6-47.5, 81.1-85.9, 44.8-54.4, 24.7-76.8 and 22.6-65.3, respectively. Whereas the removal percentage of those parameters in the natural forest system were 17.1-65.9, 49.5-51.1, 44.0-60.9, 51.1-83.5, 43.4-50.4, 28.7-58.9 and 28.3-48.0, respectively. Generally, the removal percentages within the newly planted system and the natural forest system were not significantly different. However, when the removal percentages were compared with detention time, TSS, PO4-P and TP percentages removed were significantly higher in the 7-day detention time treatment. Even though the removal percentages were highly varied and temporally dependent, the overall results showed that mangrove plantation could be used as constructed wetland for municipal wastewater treatment in a similar way to the natural mangrove system.


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