Identification and evaluation of a dominant alga from municipal wastewater in removal of nutrients

2016 ◽  
Vol 74 (11) ◽  
pp. 2727-2735 ◽  
Author(s):  
Yixuan Yang ◽  
Fei Tang ◽  
Xiaoling Su ◽  
Hua Yin ◽  
Fei Ge
Keyword(s):  
Nitrogen Source ◽  
Nutrient Removal ◽  
Municipal Wastewater ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Removal Rate ◽  
Treatment Plant ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
Low Concentrations ◽  
Gradient Gel Electrophoresis

To access better removal of nutrients with algae-based techniques, a dominant alga from real municipal wastewater was identified and its capacity in removing low concentrations of nitrogen (NH+4 or NO−3) and phosphorus (PO3−4) was evaluated. Results showed that Oedogonium brevicingulatum, a filamentous green alga, was confirmed as the dominant alga in the secondary effluent of a municipal wastewater treatment plant by polymerase chain reaction-denaturing gradient gel electrophoresis. Low concentrations of NH+4 or NO−3 (≤5 mg N L−1) and PO3−4 (≤0.5 mg P L−1) were 100% removed by the algae in a 7-d test. The maximum nutrient removal rate (Vmax) and the half-saturation constant (Km) for NH+4 (10.03 ± 0.95 mg g−1d−1 and 0.19 ± 0.03 mg L−1) and NO−3 (8.43 ± 0.21 mg g−1 d−1 and 0.27 ± 0.11 mg L−1) indicated the uptake capability for NH+4 is higher than that for NO−3. Meanwhile, it showed higher affinity for PO3−4 (Vmax: 1.42 ± 0.02 mg g−1 d−1; Km: 0.02 ± 0.00 mg L−1) with NH+4 as nitrogen source than that (Vmax: 1.24 ± 0.15 mg g−1 d−1; Km: 0.06 ± 0.03 mg L−1) with NO−3 as nitrogen source. Moreover, nutrient removal efficiencies were observed steady when nitrogen/phosphorus ratio ranged from 5:1 to 20:1. These results suggest that the dominant algae from municipal wastewater have potentials to be applied in nutrient removal.

Simultaneous removal of nitrogen and phosphorus from wastewater by means of FeS-based autotrophic denitrification

2013 ◽  
Vol 67 (12) ◽  
pp. 2761-2767 ◽  
Author(s):  
Ruihua Li ◽  
Jianmin Niu ◽  
Xinmin Zhan ◽  
Bo Liu
Keyword(s):  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Secondary Effluent ◽  
Autotrophic Denitrification ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
P Concentration ◽  
N Removal ◽  
Wide Ph Range

The efficacy of iron(II) sulfide (FeS)-based autotrophic denitrification in simultaneous nitrogen and phosphorus removal from wastewater was studied with batch experiments. It was efficient at a wide pH range of 5–9, and temperature range of 10–40 °C. The concentrations of NH4+-N, Mg2+ and HCO3− in the wastewater should be kept over 7.8, 0.24 and 30 mg L−1 for efficient nitrate (NO3−-N) reduction, respectively. The NO3−-N removal rate increased from 0 to 82 mg L−1 d−1 and then leveled off when the NO3−-N concentration increased from 0 to 415 mg L−1 and then to 700 mg L−1, respectively. The NO3−-N removal rate quickly increased, leveled off, and then sharply decreased when the PO43−-P concentration increased from 0 to 0.1 mg L−1, then to 114.0 mg L−1, and further to 683.8 mg L−1, respectively. The PO43−-P removal was over 98% when the PO43−-P concentration ranged 0–683.3 mg L−1. During treatment of the secondary effluent of a local municipal wastewater treatment plant containing NO3−-N of 14.9 mg L−1 and total phosphorus (TP) of 3.9 mg L−1, NO3−-N was reduced to 1.1 mg L−1 and TP was completely removed.

Nutrient removal performance and microbial characteristics of a full-scale IFAS-EBPR process treating municipal wastewater

2015 ◽  
Vol 73 (6) ◽  
pp. 1261-1268 ◽  
Author(s):  
Yang Bai ◽  
Yaobin Zhang ◽  
Xie Quan ◽  
Shuo Chen
Keyword(s):  
Nutrient Removal ◽  
Municipal Wastewater ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Oxygen Demand ◽  
Full Scale ◽  
Municipal Wastewater Treatment ◽  
Biological Phosphorus Removal ◽  
Microbial Characteristics ◽  
Gradient Gel Electrophoresis ◽  
Biofilm Carriers

This work describes the nutrient removal performance and microbial characteristics of a full-scale integrated fixed-film activated sludge-enhanced biological phosphorus removal (IFAS-EBPR) process for municipal wastewater treatment. The polymerase chain reaction-denaturing gradient gel electrophoresis results showed that the presence of bacteria in this process, including Nitrosomonas sp., Nitrospira sp., Nitrobacter sp., Pseudomonas sp. and Acinetobacter sp., clusters. The fluorescence in situ hybridization results implied that there were more nitrifiers and denitrifiers on the biofilm carriers than in the suspended sludge, whereas more phosphorus-accumulating organisms (PAOs) resided in the suspended sludge. With the cooperation of these functional microbial populations both on the biofilm carriers and in the suspended sludge, the chemical oxygen demand (COD), NH4+-N, total nitrogen (TN) and total phosphorus (TP) removal efficiencies were maintained at 84, 97, 70 and 81%, and the effluent concentrations of them averaged 30, 1.0, 11.5 and 0.6 mg/L, which all satisfy the Chinese discharge standard (COD <50 mg/L, NH4+-N <5 mg/L, TN <15 mg/L and TP <1 mg/L), respectively. Therefore, the IFAS-EBPR process is a reliable and effective process for nutrient removal.

WASTEWATER DISINFECTION BY UV AT TRANI MUNICIPAL PLANT

1994 ◽  
Vol 30 (4) ◽  
pp. 125-132 ◽  
Author(s):  
D. Carnimeo ◽  
E. Contini ◽  
R. Di Marino ◽  
F. Donadio ◽  
L. Liberti ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Continuous Operation ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
South Italy ◽  
Wastewater Disinfection ◽  
Pilot Investigation ◽  
Effluent Characteristics

The pilot investigation on the use of UV as an alternative disinfectant to NaOCI was started in 1992 at Trani (South Italy) municipal wastewater treatment plant (335 m3/h). The results collected after six months continuous operation enabled us to compare UV and NaOCl disinfection effectiveness on the basis of secondary effluent characteristics, quantify photoreactivation effects, evidence possible DBP formation and assess costs.

scholarly journals Assessment of Microplastics in a Municipal Wastewater Treatment Plant with Tertiary Treatment: Removal Efficiencies and Loading per Day into the Environment

Water ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1339
Author(s):  
Javier Bayo ◽  
Sonia Olmos ◽  
Joaquín López-Castellanos
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Source Control ◽  
Municipal Wastewater Treatment ◽  
Tertiary Treatment ◽  
Stable Performance ◽  
Wastewater Samples

This study investigates the removal of microplastics from wastewater in an urban wastewater treatment plant located in Southeast Spain, including an oxidation ditch, rapid sand filtration, and ultraviolet disinfection. A total of 146.73 L of wastewater samples from influent and effluent were processed, following a density separation methodology, visual classification under a stereomicroscope, and FTIR analysis for polymer identification. Microplastics proved to be 72.41% of total microparticles collected, with a global removal rate of 64.26% after the tertiary treatment and within the average retention for European WWTPs. Three different shapes were identified: i.e., microfiber (79.65%), film (11.26%), and fragment (9.09%), without the identification of microbeads despite the proximity to a plastic compounding factory. Fibers were less efficiently removed (56.16%) than particulate microplastics (90.03%), suggesting that tertiary treatments clearly discriminate between forms, and reporting a daily emission of 1.6 × 107 microplastics to the environment. Year variability in microplastic burden was cushioned at the effluent, reporting a stable performance of the sewage plant. Eight different polymer families were identified, LDPE film being the most abundant form, with 10 different colors and sizes mainly between 1–2 mm. Future efforts should be dedicated to source control, plastic waste management, improvement of legislation, and specific microplastic-targeted treatment units, especially for microfiber removal.

Enhanced Performance of Ultrafiltration Membrane with Powdered Zeolite Addition for Secondary Effluent Treatment

2013 ◽  
Vol 838-841 ◽  
pp. 2712-2716
Author(s):  
Yong Tu ◽  
Yong Gang Bai ◽  
Yong Chen ◽  
Wei Jing Liu ◽  
Jun Xu ◽  
...  
Keyword(s):  
Membrane Fouling ◽  
Fluorescence Spectra ◽  
Municipal Wastewater ◽  
Three Dimensional ◽  
Treatment Plant ◽  
Ultrafiltration Membrane ◽  
Effluent Treatment ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
Sem Images

The research on ultrafiltration membrane assisted by powdered zeolite for the treatment of secondary effluent from a municipal wastewater treatment plant was studied. The results show that membrane fouling rate is reduced by pre-coating the ultrafiltration membrane with powdered zeolite, and the treatment performance of secondary effluent is enhanced. UV-vis, three-dimensional excitation emission matrix (3D-EEM) fluorescence spectra and scanning electron microscopy (SEM) images for ultrafiltration were also discussed.

scholarly journals Upflow packed bed Anammox reactor used in two-stage deammonification of sludge digester effluent

2018 ◽  
Vol 78 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
İ. Çelen-Erdem ◽  
E. S. Kurt ◽  
B. Bozçelik ◽  
B. Çallı
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Packed Bed ◽  
Municipal Wastewater Treatment ◽  
Two Stage ◽  
N Removal ◽  
Total Nitrogen Removal

Abstract The sludge digester effluent taken from a full scale municipal wastewater treatment plant (WWTP) in Istanbul, Turkey, was successfully deammonified using a laboratory scale two-stage partial nitritation (PN)/Anammox (A) process and a maximum nitrogen removal rate of 1.02 kg N/m3/d was achieved. In the PN reactor, 56.8 ± 4% of the influent NH4-N was oxidized to NO2-N and the effluent nitrate concentration was kept below 1 mg/L with 0.5–0.7 mg/L of dissolved oxygen and pH of 7.12 ± 12 at 24 ± 4°C. The effluent of the PN reactor was fed to an upflow packed bed Anammox reactor where high removal efficiency was achieved with NO2-N:NH4-N and NO3-N:NH4-N ratios of 1.32 ± 0.19:1 and 0.22 ± 0.10:1, respectively. The results show that NH4-N removal efficiency up to 98.7 ± 2.4% and total nitrogen removal of 87.7 ± 6.5% were achieved.

Tertiary nitrification in pure oxygen moving bed biofilm reactors

2000 ◽  
Vol 41 (4-5) ◽  
pp. 361-368 ◽  
Author(s):  
L. Bonomo ◽  
G. Pastorelli ◽  
E. Quinto ◽  
G. Rinaldi
Keyword(s):  
Municipal Wastewater ◽  
Treatment Plant ◽  
Ammonia Nitrogen ◽  
Pure Oxygen ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
Typical Application ◽  
Moving Bed ◽  
Biofilm Reactors ◽  
Nitrogen Ratio

Two bench-scale reactors, fed with the secondary effluent of a municipal wastewater treatment plant (WWTP), were used in order to study tertiary nitrification in pure oxygen moving bed biofilm reactors (PO-MBBRs) with patented KMT® media as biofilm carriers. The process allowed to measure very high nitrification rates, both in ammonia limiting conditions (up to 7 gN m−2 d−1; oxygen-to-ammonia nitrogen ratio higher than 3–4 mgO2 (mgN)−1) and in oxygen limiting conditions (up to 8 gN m−2 d−1; oxygen-to-ammonia nitrogen ratio lower than 1–2 mgO2 (mgN)−1). Since the process proved flexible and reliable, it is suitable for full-scale application to municipal WWTPs. Typical application could regard, but is not limited to, tertiary nitrification of secondary effluent from existing high-purity oxygen activated sludge systems designed to achieve only organic carbon removal.

Integrated Treatment of Domestic Wastewater Using Anaerobic Filter-Constructed Wetland in Military Camps

2011 ◽  
Vol 356-360 ◽  
pp. 1331-1334
Author(s):  
Tao Lv ◽  
Wu Long Zhang ◽  
Xie Zhang ◽  
Feng Xue
Keyword(s):  
Constructed Wetland ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Domestic Wastewater ◽  
Integrated Treatment ◽  
Municipal Wastewater Treatment ◽  
Anaerobic Filter ◽  
Integrated Technique ◽  
Packing Layer

This paper studies through project cases the treatment effect of integrated technique of anaerobic filter and constructed wetland on domestic wastewater in military camps, and the method for preventing the blocking of anaerobic filter and constructed wetland packing. The results show that its average removal rate of COD, NH4+-N, TP and SS is 83.1%, 37.5%, 49.8% and 91.5% respectively, with effluent meeting the standard of Grade II in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB18918-2002); as planned, two anaerobic filters, one for operation and the other laying fallow at a alternate period of 6 months, in combination of the design of spoil disposal, can effectively prevent the packing layer from being blocked; being preprocessed, anaerobic filter can effectively prevent the packing layer of constructed wetland from being blocked; in case of a certain difference in elevation, the integrated technique can achieve unpowered operation. Besides, it is easy to implement and manage at a low operational cost without professional technician, and can treat decentralized domestic wastewater, therefore, is suitable for camps.

scholarly journals Quantifying Community Dynamics of Nitrifiers in Functionally Stable Reactors

2007 ◽  
Vol 74 (1) ◽  
pp. 286-293 ◽  
Author(s):  
Lieven Wittebolle ◽  
Han Vervaeren ◽  
Willy Verstraete ◽  
Nico Boon
Keyword(s):  
Dominant Species ◽  
Municipal Wastewater ◽  
Community Dynamics ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Batch Reactor ◽  
Dominant Role ◽  
Treatment Plant ◽  
Sampling Period ◽  
Rate Of Change ◽  
Municipal Wastewater Treatment

ABSTRACT A sequential batch reactor (SBR) and a membrane bioreactor (MBR) were inoculated with the same sludge from a municipal wastewater treatment plant, supplemented with ammonium, and operated in parallel for 84 days. It was investigated whether the functional stability of the nitrification process corresponded with a static ammonia-oxidizing bacterial (AOB) community. The SBR provided complete nitrification during nearly the whole experimental run, whereas the MBR showed a buildup of 0 to 2 mg nitrite-N liter−1 from day 45 until day 84. Based on the denaturing gradient gel electrophoresis profiles, two novel approaches were introduced to characterize and quantify the community dynamics and interspecies abundance ratios: (i) the rate of change [Δ t (week)] parameter and (ii) the Pareto-Lorenz curve distribution pattern. During the whole sampling period, it was observed that neither of the reactor types maintained a static microbial community and that the SBR evolved more gradually than the MBR, particularly with respect to AOB (i.e., average weekly community changes of 12.6% ± 5.2% for the SBR and 24.6% ± 14.3% for the MBR). Based on the Pareto-Lorenz curves, it was observed that only a small group of AOB species played a numerically dominant role in the nitritation of both reactors, and this was true especially for the MBR. The remaining less dominant species were speculated to constitute a reserve of AOB which can proliferate to replace the dominant species. The value of these parameters in terms of tools to assist the operation of activated-sludge systems is discussed.

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