scholarly journals Bacterial community diversity in a full scale biofilter treating wastewater odor

2018 ◽  
Vol 77 (8) ◽  
pp. 2014-2022 ◽  
Author(s):  
M. J. Allievi ◽  
D. D. Silveira ◽  
M. E. Cantão ◽  
P. B. Filho
Keyword(s):  
Microbial Community ◽  
Bacterial Community ◽  
Operating Time ◽  
Full Scale ◽  
Community Diversity ◽  
Sequencing Analysis ◽  
Rrna Sequencing ◽  
Odor Treatment ◽  
Spatial Depth ◽  
Empty Bed Residence Time

Abstract Constantly, the odors coming from sewage plants are considered a problem by the population. The purpose of this study was to evaluate the microbial community present in a full scale biofilter used for odor treatment. The filter was packed with peat. The main gas treated was hydrogen sulphide (H2S). The removal efficiency reached 99%, with an empty bed residence time of 30 seconds. Molecular analysis can enhance our understanding of the microbial communities in biofilters treating wastewater odor. The analysis made to characterize microbial community was High-throughput 16S rRNA sequencing analysis MiSeq® Illumina. The sampling, carried out in the year 2015, was seasonal (summer and winter) and spatial (depth and position in the biofilter). In this study, a total of 206,174 raw sequence reads for six samples were analyzed using Mothur software (v 1.33.3) based on MiSeq SOP protocol. After Mothur analysis, the results of the bacterial community were explored at the Phylum and Genus levels. In this study, the efficiency removal of hydrogen sulfide reached values greater than 99% during the monitoring, and the main bacterial genera found were Acidotermus, Telmatobacter, Methylovirgula and Bryobacter representing the bacterial community active in the transformation of H2S into a system with long operating time.

scholarly journals Interaction between typical sulfonamides and bacterial diversity in drinking water

2018 ◽  
Vol 16 (6) ◽  
pp. 914-920 ◽  
Author(s):  
Qing Wu ◽  
Shuqun Li ◽  
Xiaofei Zhao ◽  
Xinhua Zhao
Keyword(s):  
Drinking Water ◽  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Bacterial Diversity ◽  
Microbial Community Structure ◽  
Antibiotic Use ◽  
Community Diversity ◽  
Wide Range ◽  
Growth Of Bacteria

Abstract The abuse of antibiotics is becoming more serious as antibiotic use has increased. The sulfa antibiotics, sulfamerazine (SM1) and sulfamethoxazole (SMZ), are frequently detected in a wide range of environments. The interaction between SM1/SMZ and bacterial diversity in drinking water was investigated in this study. The results showed that after treatment with SM1 or SMZ at four different concentrations, the microbial community structure of the drinking water changed statistically significantly compared to the blank sample. At the genus level, the proportions of the different bacteria in drinking water may affect the degradation of the SM1/SMZ. The growth of bacteria in drinking water can be inhibited after the addition of SM1/SMZ, and bacterial community diversity in drinking water declined in this study. Furthermore, the resistance gene sul2 was induced by SM1 in the drinking water.

Heterotrophic Bacterial Community Structure of Multistage Biofilters in a Commercial Pufferfish Takifugu rubripes RAS

2013 ◽  
Vol 726-731 ◽  
pp. 1621-1627 ◽  
Author(s):  
Zhu Chen ◽  
Ying Liu ◽  
Liang Zi Liu ◽  
Xiao Jing Wang ◽  
Zhi Pei Liu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Heterotrophic Bacteria ◽  
Diversity Index ◽  
Community Diversity ◽  
Takifugu Rubripes ◽  
Recirculating Aquaculture ◽  
Microbial Resources

The success of a recirculating aquaculture system (RAS) greatly depends on the structure, dynamics and activities of microbial community. Heterotrophic bacteria as the major members play various roles. The heterotrophic bacterial community structure in threestaged biofilters was studied using four different media. 228 isolates belonging to 77species were obtained and affiliated toGammaproteobacteria,Alphaproteobacteria,Bacteroidetes,Firmicutes,ActinobacteriaandBetaproteobacteria.Gammaproteobacteriawas the predominant group. The concurrence was found between potential pathogens (VibrioandShewanella) and probiotics (BacillusandPseudomonas). On the basis of community diversity index, we could infer that differences existed between stages, and the diversity index increased along the biofilters. A comprehensive understanding of microbial community in RAS will be in favor of utilization of microbial resources and optimizing the culture systems' operation.

scholarly journals Phyllosphere community assembly and response to drought stress on common tropical and temperate forage grasses

2021 ◽  
Author(s):  
Emily K. Bechtold ◽  
Stephanie Ryan ◽  
Sarah E. Moughan ◽  
Ravi Ranjan ◽  
Klaus Nüsslein
Keyword(s):  
Microbial Community ◽  
Drought Stress ◽  
Bacterial Community ◽  
Community Assembly ◽  
Community Diversity ◽  
Grass Species ◽  
Severe Drought ◽  
Hormone Production ◽  
Plant Host ◽  
Bacterial Community Diversity

Grasslands represent a critical ecosystem important for global food production, soil carbon storage, and water regulation. Current intensification and expansion practices add to the degradation of grasslands and dramatically increase greenhouse gas emissions and pollution. Thus, new ways to sustain and improve their productivity are needed. Research efforts focus on the plant-leaf microbiome, or phyllosphere, because its microbial members impact ecosystem function by influencing pathogen resistance, plant hormone production, and nutrient availability through processes including nitrogen fixation. However, little is known about grassland phyllospheres and their response to environmental stress. In this study, globally dominant temperate and tropical forage grass species were grown in a greenhouse under current climate conditions and drought conditions that mimic future climate predictions to understand if (i) plant host taxa influence microbial community assembly, (ii) microbial communities respond to drought stress, and (iii) phyllosphere community changes correlate to changes in plant host traits and stress-response strategies. Community analysis using high resolution sequencing revealed Gammaproteobacteria as the dominant bacterial class, which increased under severe drought stress on both temperate and tropical grasses while overall bacterial community diversity declined. Bacterial community diversity, structure, and response to drought were significantly different between grass species. This community dependence on plant host species correlated with differences in grass species traits, which became more defined under drought stress conditions, suggesting symbiotic evolutionary relationships between plant hosts and their associated microbial community. Further understanding these strategies and the functions microbes provide to plants will help us utilize microbes to promote agricultural and ecosystem productivity in the future.

scholarly journals Assembly Processes and Co-occurrence Patterns of Abundant and Rare Bacterial Community in the Eastern Indian Ocean

2021 ◽  
Vol 12 ◽  
Author(s):  
Liuyang Li ◽  
Laxman Pujari ◽  
Chao Wu ◽  
Danyue Huang ◽  
Yuqiu Wei ◽  
...  
Keyword(s):  
Microbial Community ◽  
Indian Ocean ◽  
Bacterial Community ◽  
Variable Selection ◽  
Rare Species ◽  
Abundant Species ◽  
Community Diversity ◽  
Eastern Indian Ocean ◽  
Rare Taxa ◽  
Deterministic Processes

Microbial communities are composed of many rare species and a few abundant species. Considering the disproportionate importance of rare species for ecosystem functioning, it is important to understand the mechanisms structuring the rare and abundant components of a diverse community in response to environmental changes. Here, we used a 16S ribosomal RNA gene sequencing approach to investigate the bacterial community diversity in the Eastern Indian Ocean (EIO) during the monsoon and intermonsoon. We employed a phylogenetic null model and network analysis to evaluate the assembly processes and co-occurrence pattern of the microbial community. We found that higher bacterial diversity was detected in the intermonsoon with high temperature and low Chlorophyll a concentrations and N/P ratios. The balance between ecological deterministic processes and stochastic processes varied with seasons in the EIO. Meanwhile, conditionally rare taxa (CRT) were more likely modulated by variable selection processes than always rare taxa (ART) and abundant taxa (AT) (CRT > ART > AT). By linking assembly process and species co-occurrence, we demonstrated that the microbial co-occurrence associations tended to be higher when deterministic processes (mainly variable selection) were weaker. This negative trend was observed in rare species rather than abundant species. The linkage could enhance our understanding of the underlying mechanisms underpinning the generation and maintenance of microbial community diversity.

scholarly journals Microbial community diversity of Jinghong laying hens at peak production based on 16S rRNA sequencing

2018 ◽  
Vol 46 (1) ◽  
pp. 1430-1436 ◽  
Author(s):  
Shijun Fu ◽  
Shijin Guo ◽  
Jianjun Wang ◽  
Yumao Wang ◽  
Zhimei Zhang ◽  
...  
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Laying Hens ◽  
Community Diversity ◽  
16S Rrna Sequencing ◽  
Microbial Community Diversity ◽  
Rrna Sequencing

scholarly journals The Bacterial Community Diversity of Bathroom Hot Tap Water Was Significantly Lower Than That of Cold Tap and Shower Water

2021 ◽  
Vol 12 ◽  
Author(s):  
Chiqian Zhang ◽  
Ke Qin ◽  
Ian Struewing ◽  
Helen Buse ◽  
Jorge Santo Domingo ◽  
...  
Keyword(s):  
Water Quality ◽  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Water Temperature ◽  
Water Samples ◽  
Tap Water ◽  
Community Diversity ◽  
Community Structures ◽  
Bacterial Community Diversity

Microbial drinking water quality in premise plumbing systems (PPSs) strongly affects public health. Bacterial community structure is the essential aspect of microbial water quality. Studies have elucidated the microbial community structure in cold tap water, while the microbial community structures in hot tap and shower water are poorly understood. We sampled cold tap, hot tap, and shower water from a simulated PPS monthly for 16 consecutive months and assessed the bacterial community structures in those samples via high-throughput sequencing of bacterial 16S rRNA genes. The total relative abundance of the top five most abundant phyla (Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, and Firmicutes) was greater than 90% among the 24 identified phyla. The most abundant families were Burkholderiaceae, Sphingomonadaceae, unclassified Alphaproteobacteria, unclassified Corynebacteriales, and Mycobacteriaceae. A multiple linear regression suggests that the bacterial community diversity increased with water temperature and the age of the simulated PPS, decreased with total chlorine residual concentration, and had a limited seasonal variation. The bacterial community in hot tap water had significantly lower Shannon and Inverse Simpson diversity indices (p < 0.05) and thus a much lower diversity than those in cold tap and shower water. The paradoxical results (i.e., diversity increased with water temperature, but hot tap water bacterial community was less diverse) were presumably because (1) other environmental factors made hot tap water bacterial community less diverse, (2) the diversity of bacterial communities in all types of water samples increased with water temperature, and (3) the first draw samples of hot tap water could have a comparable or even lower temperature than shower water samples and the second draw samples of cold tap water. In both a three-dimensional Non-metric multidimensional scaling ordination plot and a phylogenetic dendrogram, the samples of cold tap and shower water cluster and are separate from hot tap water samples (p < 0.05). In summary, the bacterial community in hot tap water in the simulated PPS had a distinct structure from and a much lower diversity than those in cold tap and shower water.

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