Application of Moringa oleifera Lam. fractionated proteins for inactivation of Escherichia coli from water

2020 ◽  
Vol 81 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Fabiane Masala R. Silveira ◽  
Aline Takaoka A. Baptista ◽  
Tatiane V. Dutra ◽  
Benício Alves de Abreu Filho ◽  
Raquel G. Gomes ◽  
...  

Abstract Chemical products traditionally used in the disinfection of water bodies often pose human health risks. For this reason, studies on natural coagulants such as Moringa oleifera Lam. represent an alternative for the inactivation of pathogenic microorganisms, among which is Escherichia coli. This study evaluated the effect of different concentrations of coagulants obtained from Moringa seed extracts and their protein fractions in the inactivation of E. coli during the coagulation/flocculation process. The coagulants studied were the aqueous extract, saline extract and protein fractions albumin and globulin, highlighting that the protein fractions were more effective on inactivating E. coli. The protein fraction globulin at a concentration of 10.0 mg L−1 showed bactericidal effects against E. coli within 18 min, whereas the albumin showed a bacteriostatic effect within 48 min because it isolated colonies in the sediment sample.

Author(s):  
Reinaldo Romero Vargas ◽  
Marise Cotta Machado ◽  
Maria Aparecida Da Silva ◽  
Thiago Engels

The seed extracts of Moringa oleifera has been studied as alternative coagulating agent to chemicals commonly used in order to minimize costs while reducing environmental impacts and human health arising from traditional processes of wastewater treatment. This work studied the performance of the aqueous extract of Moringa oleifera seed in the removal of four different types of textile dyes classified as dispersed, acidic, basic and reactive. Studies with aqueous and saline solutions were performed aiming to improve performance in the removal of textile dyes, and stability testing on the storage time of the extract of Moringa oleifera. Studies were performed using spectrophotometric analysis by means of calibration curves of textile dyes in an aqueous medium.The results showed that the seed extract of Moringa oleifera was quite efficient in the removal of textile dyes, except for the cationic type. Regarding the behavior of textile dyes in aqueous solution and the saline extract were not found significant differences in terms of removal. Other promising results were obtained to evaluate coagulant activity of the extract up to three days storage at room temperature. This fact is important from the operational aspect arising from the use of the extract on a large scale: the possibility to prepare a larger amount of extract, without loss of coagulant activity may enable the utilization of this resource


2020 ◽  
Vol 9 (4) ◽  
pp. e164943041
Author(s):  
Semirames do Nascimento Silva ◽  
Eliezer da Cunha Siqueira ◽  
Josivanda Palmeira Gomes ◽  
Roberta de Oliveira Sousa Wanderley ◽  
Polyana Barbosa Silva

O objetivo deste trabalho foi avaliar a qualidade química e microbiológica da água de cisternas antes e após o tratamento com sementes de Moringa ou leifera Lam. Como sementes de moringa usadas no trabalho foram compostas no município de Cajazeiras. O coagulante da moringa foi usado em meio aquoso. A água filtrada na obra veio de cisternas construídas no assentamento de Santo Antônio, município de Cajazeiras. A água utilizada foi analisada antes e após o tratamento.Uma análise dos parâmetros: pH, condutividade elétrica, alcalinidade total, provável resíduo e total de dissolvidos foi realizada no Laboratório de Água e Solo e no Laboratório de Microbiologia do Instituto Federal de Educação, Ciência e Tecnologia do Campus da Paraíba, análises de coliformes fecais e Escherichia coli foram mantidos. Todas as estatísticas de substâncias químicas mostram diferenças antes e depois do tratamento. Os controles C1 e C5 apresentam presença de Escherichia coli , os demais são causados pela E. coli .


Water ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 2731
Author(s):  
Nury Infante ◽  
Refugio Rodríguez ◽  
Yaneth Bartolo ◽  
Olga Sánchez ◽  
Isabel Sanz ◽  
...  

Cork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cork) on the inhibition of Escherichia coli (InhEc). The LacZ gene from a strain of E. coli was used as the target sequence using viability quantification Polymerase Chain Reaction (qPCR) and differentiation of viable and dead bacteria through selective cell viability PMA staining. To perform this, a 27−4 fractional factorial design and a biofiltration system were used to evaluate the effect of the active protein in MoSe immobilized in granulated cork on InhEc. We found that the potential for antimicrobial activity increased with f-cork for an effective maximal bacterial reduction (99.99%; p < 0.05). The effect of f-cork functionalized with MoSe on E. coli viability was of 0.024% and 0.005% for the cells exposed to PMA, respectively, being the relevant conditions in treatment 2: (0 L/min) without aeration, (5%) MoSe and (5 mm) cork particle. In conclusion, the f-cork functionalized with MoSe presented biosorbent and antibacterial properties that effectively reduced the E. coli growth.


Author(s):  
Md. Shahin Azad ◽  
Syaza Azhari ◽  
Mohd Sukri Hassan

The utilization of biopolymer derived from Moringa oleifera bark using ZnCl2 and H2SO4 as activating agents for eliminating Methylene blue, Escherichia coli and Pseudomonas aeruginosa from producing wastewater. In this study, Methylene blue and both bacteria were effectively adsorbed by activated carbon with lowest dosage. The activated carbon was prepared from natural-by product of Moringa oleifera bark by pyrolysis in a furnace at 700°C for 1 h. The characteristics of activated carbon have been determined using Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET), pHzpc (zero point charge), and FTIR spectroscopy. The obtained result were closely fitted with Freundlich isotherm model and adsorption kinetics follow the pseudo-second order model with the highest value of correlation coefficient (R2~1). Adsorption quantity was dose dependent and bacteria were maximum adsorbed using 10 mg of activated carbon as well as 25mg for methylene blue. The maximum adsorption capacity showed within 1 hour. The bacterial load was reduced by 98% for E. coli, 96% for P. aeruginosa as well as methylene blue reduced 94.2% from aqueous solution using batch adsorption methods. Adsorption process controlled by film diffusion mechanism. These result proposed that the activated carbon of Moringa oleifera can be used as a good adsorbent for the removal of Methylene blue, E. coli and P. aeruginosa.


2019 ◽  
Vol 7 (2) ◽  
pp. 13 ◽  
Author(s):  
Claire Stephane Metsopkeng ◽  
Chretien Lontsi Djimeli ◽  
Olive Vivien Noah Ewoti ◽  
Lucienne Marlyse Moungang ◽  
Paul Alain Nana ◽  
...  

This study aimed to evaluate in microcosm condition, the survival of Aeromonas hydrophila and Enteropathogenic Escherichia coli (EPEC), in the presence of M. oleifera aqueous seeds extract at concentrations varying from 1 to 40 g/L, and under 4 °C and 23 °C incubation temperature. It has been noted that cell abundances decrease gradually with the increasing in the seeds extract concentration. However, a marked cells regrowth was sometimes noted. In monospecies cell incubation condition, under 4 °C, the EPEC cells inhibition percentages (CIP) values varied from 52.12 to 99.84%. Those of A. hydrophila varied from 13.2 to 96%. The lowest CIPs were noted at the extract concentration 1g/L for EPEC and A. hydrophila. The highest CIP value was registered at 10 and 40 g/L for EPEC and at 15 g/L for A. hydrophila. Under 23 °C incubation, the EPEC CIPs values varied from 74.04 to 99.9% and those of A. hydrophila varied from 21.2 to 97.8%. For E. coli, the lowest and the highest CIP were recorded at the extract concentration 1g/L and 30 g/L, respectively. In bispecies cells incubation condition, the CIPs were relatively different. These results show the potential exploitation of M. oleifera extracts in the microbiological treatment of potable water.


Author(s):  
Maiara Luzia Grigoli OLIVIO ◽  
Elizângela Flávia Jacob ESTEVES ◽  
Dagmar Aparecida de Marco FERRO

Comumente o sulfato de alumínio é o coagulante químico utilizado nas Estações de Tratamentos para a clarificação da água, embora produza um lodo tóxico de difícil deposição no ambiente. No entanto, os coagulantes naturais como sementes de Moringa oleífera Lam. têm demonstrado vantagens para tratamento em comparação aos químicos, pela simplicidade de uso e baixo custo. O estudo objetivou analisar a eficiência coagulante da semente de Moringa oleífera na clarificação de água com turbidez e comparar com os parâmetros do coagulante químico sulfato de alumínio. As amostras de água foram coletadas no Córrego Jacu Queimado e as sementes colhidas em um plantio da própria espécie. Foram realizados, em laboratório, etapas sequenciais do experimento: comparação da eficiência da semente verde e seca, determinação da concentração e tempo de exposição da semente à água, comparação de agitação manual e magnética entre elas e do coagulante biológico com o químico, sendo analisados os aspectos físico, químico e biológico antes e após a adição de ambos. Observou-se que apenas a semente seca realiza a clarificação da água em comparação com os resultados das análises da água clarificada com o sulfato, determinando-se que 5 sementes são eficazes para tratar 1 litro de água com turbidez de até 25,0 NTU, considerando-se mais relevante a agitação manual para o efeito de coagulação e o aguardo de 2 horas para o término da decantação. Conclui-se, que a semente de Moringa é um coagulante biológico que se apresenta como alternativa para o tratamento de água com turbidez, porém, ainda não é indicada para a potabilidade. É preciso dar continuidade ao tratamento em busca de processos que visam melhorar a qualidade da água para aproximar-se aos parâmetros aceitáveis para o consumo humano.   Moringa oleífera Lam SEEDS: EFFICIENT USE OF BIOLOGICAL COAGULANT FOR WATER TURBIDITY TREATMENT, COMPARED TO ALUMINUM SULFATE CHEMICAL COAGULANT   ABSTRACT Aluminum sulfate is a chemical coagulant commonly used at Water Treatment Plants for water clarification, although it produces a toxic sludge of difficult deposition in the environment. However, natural coagulants such as Moringa oleíferaLam seeds have evidenced the advantages for the treatment when compared to chemicals, due to either their simplicity in and low cost.  This research aims at reviewing the efficient use of   Moringa oleífera seeds as coagulants for turbid water clarification as well as comparing the parameters of aluminum sulfate chemical coagulants. The water samples were collected at Córrego Jacu Queimado and the seeds were harvested from a crop of its own species. Sequential steps were performed in the lab for this experiment: Comparison of green and dry seeds efficiency, concentration and time of exposition of the seed to water measurements, comparison of manual and magnetic stirring among them and among biological and chemical coagulants, being analyzed physical, chemical and biological aspects before and after adding both of them. It was observed that only the dry seed enables water clarification when comparing the analysis results to sulfate water clarification, it was ascertained that 5 seeds are efficient to treat a liter of 25,0 NTU turbidity in water, considering as more relevant manual stirring for coagulation effect after a two-hour rest for decantation completion. It was concluded that Moringa seed is a biological coagulant presented as an alternative for turbid water treatment, although it is not indicated for drinkability. Further treatment is necessary in order to keep searching for processes that target to improve the quality of water and meeting acceptable parameters for water consumption.   Keywords: Biological Coagulant. Chemical Coagulant. Efficiency. Water Clarification. Comparative Parameters.


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