Allelopathic effect of rhubarb extracts on the growth of Microcystis aeruginosa

Abstract With its advantages of ecological safety, environmental affinity, and high selectivity, allelopathic technology has been widely developed for algae inhibition. However, obtaining effective allelochemicals and realizing their mechanism are difficult. In this paper, a Chinese herbal medicine, namely, Rheum palmatum L. (Chinese rhubarb), was utilized as a source of allelopathic substances for the first time. Four units of rhubarb organic extracts were collected to study the inhibition of growth, photosynthesis, proteins, and algal toxin of Microcystis aeruginosa. Results showed that the ethyl acetate, n-butanol, and aqueous phases of the rhubarb extracts have notable inhibitory effects. After a 16-day treatment, the four extracts reduced M. aeruginosa by 64.1%, 59.3%, 61.9%, and 7.2% with disruption of algal photosynthesis and protein synthesis and reduction of algal toxin.

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Inhibitory effects of Pontederia cordata on the growth of Microcystis aeruginosa

Water Science & Technology ◽

10.2166/wst.2018.090 ◽

2018 ◽

Vol 2017 (1) ◽

pp. 99-107 ◽

Cited By ~ 2

Author(s):

Yanping Qian ◽

Ning Xu ◽

Juan Liu ◽

Runan Tian

Keyword(s):

Microcystis Aeruginosa ◽

Inhibitory Effect ◽

Butyl Alcohol ◽

Inhibition Rate ◽

Inhibitory Effects ◽

Fibrous Root ◽

Pontederia Cordata ◽

Algal Inhibition ◽

Organic Extracts ◽

Acetone Extracts

Abstract This study investigated the effect of Pontederia cordata on Microcystis aeruginosa growth in three different experimental settings: (i) co-cultivation, (ii) exposure of cyanobacteria to culture water of P. cordata, and (iii) exposure of M. aeruginosa to organic extracts of P. cordata. Results showed that the growth of M. aeruginosa was significantly inhibited by co-cultivation, with the highest inhibition rate of 61.9% within 5 days. Moreover, 95% culture water with P. cordata could markedly inhibit the growth of M. aeruginosa, with inhibition rate of 98.3% on day 6, indicating that most of the algal cells died. The organic extracts of fibrous root showed stronger inhibition effect than the leaf and stalk extracts. Acetone extract of fibrous root showed the strongest inhibitory effect on M. aeruginosa. Different components of 80% acetone extracts from fibrous root exhibited varied effects on the growth of M. aeruginosa. Ethyl acetate and water components had strong inhibition effects on M. aeruginosa. By contrast, n-butyl alcohol components had weak inhibition effects, and hexane components even promoted the growth of M. aeruginosa. Allelochemicals of P. cordata were primarily released into the water through the fibrous root. Results indicated that P. cordata can be applied in environmentally friendly algal inhibition.

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Allelopathic effect of Chitosan Fiber on the growth of Microcystis aeruginosa

E3S Web of Conferences ◽

10.1051/e3sconf/202020602012 ◽

2020 ◽

Vol 206 ◽

pp. 02012

Author(s):

Jingguo Cao ◽

Hongyan Zhao ◽

Zezhang Dong ◽

Zongzheng Yang

Keyword(s):

Microcystis Aeruginosa ◽

Cyanobacterial Bloom ◽

Allelopathic Effect ◽

Environment Protection ◽

Inhibition Rate ◽

Algal Toxins ◽

Safety Protection ◽

Novel Method ◽

Chitosan Fiber ◽

First Time

Cyanobacterial bloom elimination has become an urgent concern in aquatic environment protection. Allelopathic technology, however, is one of the potential effective approaches because of its exclusive and ecological safety protection. The potential application of chitosan fiber in the inhibition of Microcystis aeruginosa was explored for the first time. Results showed that the cell growth of M. aeruginosa was inhibited effectively by chitosan fibers. After 12 days of treatment, the inhibition rate (IR) of algal cells was above 78.9%; those of chlorophyll a and carotenoids in algal cells were 86.9% and 93.1%, respectively; those of phycocyanin, allophycocyanin and phycoerythrin in phycobiliproteins were all approximately 100%; those of soluble proteins and algal toxins were 88% and 100%, respectively; and those of superoxide dismutase and catalase activities were 68% and 74%, respectively. In addition, chitosan fiber effectively destroyed the structure of algal cells involved in the photosynthetic process, thereby destroying the metabolic activity of algal cells. This study provides a novel method utilizing a new allelopathic material.

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Inhibitory effects of Cyperus alternifolius on growth of Microcystis aeruginosa and identification of algicidal substances

Allelopathy Journal ◽

10.26651/allelo.j/2019-46-1-1199 ◽

2019 ◽

Vol 46 (1) ◽

pp. 73-84

Author(s):

L. Zhou ◽

S. Nakai ◽

G. F. Chen ◽

Q. Pan ◽

N. X. Cui ◽

...

Keyword(s):

Microcystis Aeruginosa ◽

Inhibitory Effects ◽

Cyperus Alternifolius

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Allelopathic Effect of Serphidium kaschgaricum (Krasch.) Poljak. Volatiles on Selected Species

Plants ◽

10.3390/plants10030495 ◽

2021 ◽

Vol 10 (3) ◽

pp. 495

Author(s):

Shixing Zhou ◽

Toshmatov Zokir ◽

Yu Mei ◽

Lijing Lei ◽

Kai Shi ◽

...

Keyword(s):

Volatile Compounds ◽

Volatile Oil ◽

Amaranthus Retroflexus ◽

Allelopathic Effect ◽

Chemical Profile ◽

Volatile Oils ◽

Phytotoxic Activity ◽

Volatile Organic ◽

First Time ◽

Stem Leaf

The chemical profile and allelopathic effect of the volatile organic compounds (VOCs) produced by a dominant shrub Serphidium kaschgaricum (Krasch.) Poljak. growing in northwestern China was investigated for the first time. Serphidium kaschgaricu was found to release volatile compounds into the surroundings to affect other plants’ growth, with its VOCs suppressing root elongation of Amaranthus retroflexus L. and Poa annua L. by 65.47% and 60.37% at 10 g/1.5 L treatment, respectively. Meanwhile, volatile oils produced by stems, leaves, flowers and flowering shoots exhibited phytotoxic activity against A. retroflexus and P. annua. At 0.5 mg/mL, stem, leaf and flower oils significantly reduced seedling growth of the receiver plants, and 1.5 mg/mL oils nearly completely prohibited seed germination of both species. GC/MS analysis revealed that among the total 37 identified compounds in the oils, 19 of them were common, with eucalyptol (43.00%, 36.66%, 19.52%, and 38.68% in stem, leaf, flower and flowering shoot oils, respectively) and camphor (21.55%, 24.91%, 21.64%, and 23.35%, respectively) consistently being the dominant constituents in all oils. Eucalyptol, camphor and their mixture exhibited much weaker phytotoxicity compared with the volatile oils, implying that less abundant compounds in the volatile oil might contribute significantly to the oils’ activity. Our results suggested that S. kaschgaricum was capable of synthesizing and releasing allelopathic volatile compounds into the surroundings to affect neighboring plants’ growth, which might improve its competitiveness thus facilitate the establishment of dominance.

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A novel role for WAVE1 in controlling actin network growth rate and architecture

Molecular Biology of the Cell ◽

10.1091/mbc.e14-10-1477 ◽

2015 ◽

Vol 26 (3) ◽

pp. 495-505 ◽

Cited By ~ 9

Author(s):

Meredith O. Sweeney ◽

Agnieszka Collins ◽

Shae B. Padrick ◽

Bruce L. Goode

Keyword(s):

Actin Filament ◽

Specific Activity ◽

Inhibitory Effect ◽

Actin Network ◽

Inhibitory Effects ◽

Cellular Functions ◽

Network Growth ◽

Assembly Kinetics ◽

Filament Networks ◽

First Time

Branched actin filament networks in cells are assembled through the combined activities of Arp2/3 complex and different WASP/WAVE proteins. Here we used TIRF and electron microscopy to directly compare for the first time the assembly kinetics and architectures of actin filament networks produced by Arp2/3 complex and dimerized VCA regions of WAVE1, WAVE2, or N-WASP. WAVE1 produced strikingly different networks from WAVE2 or N-WASP, which comprised unexpectedly short filaments. Further analysis showed that the WAVE1-specific activity stemmed from an inhibitory effect on filament elongation both in the presence and absence of Arp2/3 complex, which was observed even at low stoichiometries of WAVE1 to actin monomers, precluding an effect from monomer sequestration. Using a series of VCA chimeras, we mapped the elongation inhibitory effects of WAVE1 to its WH2 (“V”) domain. Further, mutating a single conserved lysine residue potently disrupted WAVE1's inhibitory effects. Taken together, our results show that WAVE1 has unique activities independent of Arp2/3 complex that can govern both the growth rates and architectures of actin filament networks. Such activities may underlie previously observed differences between the cellular functions of WAVE1 and WAVE2.

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Morin Exerts Anti-Diabetic Effects in Human HepG2 Cells Via Down-Regulation of miR-29a

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/a-0650-4082 ◽

2018 ◽

Vol 127 (09) ◽

pp. 615-622 ◽

Cited By ~ 2

Author(s):

Toktam Razavi ◽

Shideh Montasser Kouhsari ◽

Khalil Abnous

Keyword(s):

Insulin Signaling ◽

High Glucose ◽

Hepg2 Cells ◽

Target Genes ◽

Expression Level ◽

Inhibitory Effects ◽

Down Regulation ◽

Insulin Mimetic ◽

Important Regulator ◽

First Time

Abstract Diabetes mellitus is a complex metabolic disease around the world that is characterized by hyperglycemia resulting from impaired insulin secretion, insulin action, or both. MicroRNA-29a is an important regulator of insulin signaling and gluconeogenesis pathways through IRS2, PI3K and PEPCK expressions which up regulates in Diabetes. Morin is a substantial bioflavonoid which has insulin mimetic effect, and interacting with nucleic acids and proteins. In this study HepG2 cells, were exposed to high glucose to induce diabetic condition. We have determined whether high glucose stimulation might promotes miR-29a expression level in HepG2 cells and subsequently evaluated the Morin treatment effects on this state. In HepG2 cells, high glucose increases miR-29a expression level and decreases its target genes, IRS2 and PI3K expression, and increases associated downstream gene in gluconeogenic pathway, PEPCK. Morin treatment down regulates miR-29a expression level and improves insulin signaling and glucose metabolism. To confirm the inhibitory effects of Morin on miR-29a, we have transfected cells with mimic and inhibitor-miR-29a. This study for the first time identifies that Morin improves diabetic condition through down regulation of the miR-29a level, and suggest that this new inhibitor of miR-29a may be a useful biomedicine to treat diabetes.

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Allelopathic effect of Azadirachta indica L. on the germination of Abelmoschus esculentus L.

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.15.13 ◽

2014 ◽

Vol 15 ◽

pp. 13-22 ◽

Cited By ~ 1

Author(s):

T. Vaithiyanathan ◽

M. Soundari ◽

M. Rajesh ◽

K. Sankar Ganesh ◽

P. Sundaramoorthy

Keyword(s):

Azadirachta Indica ◽

Photosynthetic Pigment ◽

Research Work ◽

Chemical Compounds ◽

Abelmoschus Esculentus ◽

Allelopathic Effect ◽

Root Extract ◽

Root Bark ◽

Inhibitory Effects ◽

Leaf Extracts

The chemical compounds have been reported to be exuded by plants and their inhibitory effects of other plants. The research work was carried out to study the allelopathic effect of root, bark and leaf extracts of Azadirachta indica L. on the seed germination of Abelmoschus esculentus L. Germination studies were conducted in laboratory, the bhendi seeds treated with the root, bark and leaf extracts of Neem. On the seventh day the morphological parameters, photosynthetic pigment and biochemical contents were analysed. The result showed that the rate of germination of bhendi decreased in all Azadirachta indica L. extracts. The reduction in biochemical contents of bhendi was also noted. The highest rate of germination was observed in control (distilled water) treatment and the highest rate of inhibition was observed in root extract followed by bark and leaf extracts.

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Experimental evidence for foraminiferal calcification under anoxia

Biogeosciences ◽

10.5194/bg-11-4029-2014 ◽

2014 ◽

Vol 11 (14) ◽

pp. 4029-4038 ◽

Cited By ~ 36

Author(s):

M. P. Nardelli ◽

C. Barras ◽

E. Metzger ◽

A. Mouret ◽

H. L. Filipsson ◽

...

Keyword(s):

Environmental Variability ◽

Sediment Cores ◽

Adult Mortality ◽

Short Term ◽

Essential Information ◽

Ample Evidence ◽

Anoxic Conditions ◽

Inhibition Of Growth ◽

Foraminiferal Species ◽

First Time

Abstract. Benthic foraminiferal tests are widely used for paleoceanographic reconstructions from a range of different environments with varying dissolved oxygen concentrations in the bottom water. There is ample evidence that foraminifera can live in anoxic sediments. For some species, this is explained by a switch to facultative anaerobic metabolism (i.e. denitrification). Here we show for the first time that adult specimens of three benthic foraminiferal species are not only able to survive, but are also able to calcify under anoxic conditions, at various depths in the sediment, and with or without nitrates. In fact, several specimens of Ammonia tepida (1–4%), Bulimina marginata (8–24%) and Cassidulina laevigata (16–23%) were able to calcify at different redox fronts of sediment cores, under laboratory conditions. This demonstrates ongoing metabolic processes, even in micro-environments where denitrification is not possible. Earlier observations suggest that the disappearance of foraminiferal communities after prolonged anoxia is not due to instantaneous or strongly increased adult mortality. Here we show that it cannot be explained by an inhibition of growth through chamber addition either. Our observations of ongoing calcification under anoxic conditions mean that geochemical proxy data obtained from benthic foraminifera in settings experiencing intermittent anoxia have to be reconsidered. The analysis of whole single specimens or of their successive chambers may provide essential information about short-term environmental variability and/or the causes of anoxia.

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Natural Clovanes from the Gorgonian Coral Rumphella Antipathies

Natural Product Communications ◽

10.1177/1934578x1300800801 ◽

2013 ◽

Vol 8 (8) ◽

pp. 1934578X1300800 ◽

Cited By ~ 2

Author(s):

Hsu-Ming Chung ◽

Wei-Hsien Wang ◽

Tsong-Long Hwang ◽

Yang-Chang Wu ◽

Ping-Jyun Sung

Keyword(s):

Spectral Data ◽

Natural Source ◽

Human Neutrophils ◽

Spectroscopic Methods ◽

Superoxide Anions ◽

Inhibitory Effects ◽

Gorgonian Coral ◽

First Time

Three natural clovane-related sesquiterpenoids, 2β-acetoxyclovan-9α-ol (1), 9α-acetoxyclovan-2β-ol (2) and clovan-2β,9β-diol (3), were isolated from the gorgonian coral Rumphella antipathies. The structures of clovanes 1–3 were elucidated by spectroscopic methods and by comparison of the spectral data with those of known clovane analogues. This is the first time that clovanes 1–3 have been isolated from a natural source. Clovanes 1 and 2 displayed inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils.

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Guaiane Sesquiterpenes from the Rhizome of Curcuma xanthorrhiza and Their Inhibitory Effects on UVB-induced MMP-1 Expression in Human Keratinocytes

Natural Product Communications ◽

10.1177/1934578x1701201003 ◽

2017 ◽

Vol 12 (10) ◽

pp. 1934578X1701201

Author(s):

Ji-Hae Park ◽

Mohamed Antar Aziz Mohamed ◽

Nhan Nguyen Thi ◽

Kyeong-Hwa Seo ◽

Ye-Jin Jung ◽

...

Keyword(s):

Mrna Level ◽

Human Keratinocytes ◽

Electron Ionization Mass Spectrometry ◽

Ionization Mass ◽

Aging Effects ◽

Inhibitory Effects ◽

Chemical Structures ◽

Skin Cells ◽

Curcuma Xanthorrhiza ◽

First Time

A new guaiane sesquiterpene, (1 R,4 S,5 S,10 R)-10-methyl guaianediol (1), along with five known compounds, 10-methylalismoxide (2), (+)-alismoxide (3), isozedoarondiol (4), zedoarondiol (5), and zedoalactone B (6), were isolated from the rhizomes of Curcuma xanthorrhiza Roxb. Compounds 1-6 were isolated from this plant for the first time. Chemical structures were determined using nuclear magnetic resonance (NMR), electron ionization mass spectrometry (EI/MS), polarimetry, circular dichroism (CD), and infrared spectroscopy (IR). Compounds 3 and 4 decreased MMP-1 expression in UVB-treated human keratinocytes by about 8.9-fold and 7.6-fold at the mRNA level, and by about 9.2-fold and 6.6-fold at the protein level, respectively. Results indicate that the isolated compounds have anti-aging effects by inhibiting MMP-1 expression in skin cells.

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