The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate

Abstract The study aimed to identify interspecies interactions within a native microbial community present in a hydrogen-producing bioreactor fed with two wheat straw cultivars. The relationships between the microbial community members were studied building a canonical correspondence analysis and corroborated through in vitro assays. The results showed that the bioreactor reached a stable hydrogen production of ca. 86 mL/kg·d where the cultivar change did not affect the average performance. Lactobacillus and Clostridium dominated throughout the whole operation period where butyric acid was the main metabolite. A canonical correspondence analysis correlated positively Lactobacillus with hydrogen productivity and hydrogen-producing bacteria like Clostridium and Ruminococaceae. Agar diffusion testing of isolated strains confirmed that Lactobacillus inhibited the growth of Enterococcus, but not of Clostridium. We suggest that the positive interaction between Lactobacillus and Clostridium is generated by a division of labor for degrading the lignocellulosic substrate where Lactobacillus produces lactic acid from the sugar fermentation while Clostridium quickly uses this lactic acid to produce hydrogen and butyric acid. The significance of this work lies in the fact that different methodological approaches confirm a positive association in the duo Lactobacillus-Clostridium in a bioreactor with stable hydrogen production from a complex substrate.

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Reduced-Particle-Size Wheat Bran Is Efficiently Colonized by a Lactic Acid-Producing Community and Reduces Levels ofEnterobacteriaceaein the Cecal Microbiota of Broilers

Applied and Environmental Microbiology ◽

10.1128/aem.01343-18 ◽

2018 ◽

Vol 84 (21) ◽

Cited By ~ 6

Author(s):

Karen Vermeulen ◽

Joran Verspreet ◽

Christophe M. Courtin ◽

Freddy Haesebrouck ◽

Steve Baeyen ◽

...

Keyword(s):

Particle Size ◽

Lactic Acid ◽

Microbial Community ◽

Butyric Acid ◽

Wheat Bran ◽

Feed Additive ◽

Dietary Fibers ◽

Content Type ◽

Coa Transferase ◽

Cecal Microbiota

ABSTRACTIn the present study, we investigated whether reducing the particle size of wheat bran affects the colonizing microbial community using batch fermentations with cecal inocula from seven different chickens. We also investigated the effect of in-feed administration of regular wheat bran (WB; 1,690 μm) and wheat bran with reduced particle size (WB280; 280 μm) on the cecal microbial community composition of broilers. During batch fermentation, WB280 was colonized by a lactic acid-producing community (BifidobacteriaceaeandLactobacillaceae) and byLachnospiraceaethat contain lactic acid-consuming butyric acid-producing species. The relative abundances of theEnterobacteriaceaedecreased in the particle-associated communities for both WB and WB280 compared to that of the control. In addition, the community attached to wheat bran was enriched in xylan-degrading bacteria. When administered as a feed additive to broilers, WB280 significantly increased the richness of the cecal microbiota and the abundance of bacteria containing the butyryl-coenzyme A (CoA):acetate CoA-transferase gene, a key gene involved in bacterial butyrate production, while decreasing the abundances ofEnterobacteriaceaefamily members in the ceca. Particle size reduction of wheat bran thus resulted in the colonization of the bran particles by a very specific lactic acid- and butyric acid-producing community and can be used to steer toward beneficial microbial shifts. This can potentially increase the resilience against pathogens and increase animal performance when the reduced-particle-size wheat bran is administered as a feed additive to broilers.IMPORTANCEPrebiotic dietary fibers are known to improve the gastrointestinal health of both humans and animals in many different ways. They can increase the bulking capacity, improve transit times, and, depending on the fiber, even stimulate the growth and activity of resident beneficial bacteria. Wheat bran is a readily available by-product of flour processing and is a highly concentrated source of (in)soluble dietary fiber. The intake of fiber-rich diets has been associated with increasedFirmicutesand decreasedProteobacterianumbers. Here, we show that applying only 1% of a relatively simple substrate which was technically modified using relatively simple techniques reduces the concentration ofEnterobacteriaceae. This could imply that in future intervention studies, one should take the particle size of dietary fibers into account.

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Effects of processing, moisture, and storage length on the fermentation profile, particle size, and ruminal disappearance of reconstituted corn grain

Journal of Animal Science ◽

10.1093/jas/skaa332 ◽

2020 ◽

Vol 98 (11) ◽

Author(s):

Ana L M Gomes ◽

Antonio V I Bueno ◽

Fernando A Jacovaci ◽

Guilherme Donadel ◽

Luiz F Ferraretto ◽

...

Keyword(s):

Particle Size ◽

Lactic Acid ◽

Butyric Acid ◽

Hammer Mill ◽

Moisture Concentration ◽

Fermentation Profile ◽

And Storage ◽

Ground Corn ◽

Corn Grain

Abstract Our objective was to examine the effects of processing, moisture, and anaerobic storage length of reconstituted corn grain (RCG) on the fermentation profile, geometric mean particle size (GMPS), and ruminal dry matter disappearance (DMD). Dry corn kernels were ground (hammer mill, 5-mm screen) or rolled, then rehydrated to 30%, 35%, or 40% moisture, and stored for 0, 14, 30, 60, 90, 120, or 180 d in laboratory silos. Rolled corn had an increased GMPS compared with ground corn (2.24 and 1.13 mm, respectively, at ensiling). However, there was a trend for an interaction between processing and moisture concentration to affect particle size, with GMPS increasing with increased moisture concentration, especially in ground corn. Longer storage periods also slightly increased GMPS. Processing, moisture, and storage length interacted to affect the fermentation pattern (two- or three-way interactions). Overall, pH decreased, whereas lactic acid, acetic acid, ethanol, and NH3-N increased with storage length. RCG with 30% moisture had less lactic acid than corn with 35% and 40% moisture, indicating that fermentation might have been curtailed and also due to the clostridial fermentation that converts lactic acid to butyric acid. Ensiling reconstituted ground corn with 30% of moisture led to greater concentrations of ethanol and butyric acid, resulting in greater DM loss than grain rehydrated to 35% or 40% of moisture. Ammonia-N and in situ ruminal DMD were highest for reconstituted ground corn with 35% or 40% of moisture, mainly after 60 d of storage. Therefore, longer storage periods and greater moisture contents did not offset the negative effect of greater particle size on the in situ ruminal DMD of rolled RCG. Nonetheless, RCG should be ensiled with more than 30% moisture and stored for at least 2 mo to improve the ruminal DMD and reduce the formation of ethanol and butyric acid.

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Continuous hydrogen production and microbial community profile in the dark fermentation of tequila vinasse: Response to increasing loading rates and immobilization of biomass

Biochemical Engineering Journal ◽

10.1016/j.bej.2021.108049 ◽

2021 ◽

pp. 108049

Author(s):

Luis Arellano-García ◽

Jesús B. Velázquez-Fernández ◽

Marcos Macías-Muro ◽

Erika N. Marino-Marmolejo

Keyword(s):

Microbial Community ◽

Hydrogen Production ◽

Dark Fermentation ◽

Loading Rates ◽

Community Profile ◽

Microbial Community Profile

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Effects of spraying lactic acid and peroxyacetic acid on the quality and microbial community dynamics of vacuum skin-packaged chilled beef during storage

Food Research International ◽

10.1016/j.foodres.2021.110205 ◽

2021 ◽

Vol 142 ◽

pp. 110205

Author(s):

Jina Han ◽

Yunge Liu ◽

Lixian Zhu ◽

Rongrong Liang ◽

Pengcheng Dong ◽

...

Keyword(s):

Lactic Acid ◽

Microbial Community ◽

Community Dynamics ◽

Peroxyacetic Acid ◽

Microbial Community Dynamics

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RS5 Produced More Butyric Acid through Regulating the Microbial Community of Human Gut Microbiota

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.0c08187 ◽

2021 ◽

Vol 69 (10) ◽

pp. 3209-3218

Author(s):

Renbing Qin ◽

Jin Wang ◽

Chen Chao ◽

Jinglin Yu ◽

Les Copeland ◽

...

Keyword(s):

Microbial Community ◽

Gut Microbiota ◽

Butyric Acid ◽

Human Gut ◽

Human Gut Microbiota

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Correspondence analysis and canonical correspondence analysis

Statistics for Biology and Health - Analysing Ecological Data ◽

10.1007/978-0-387-45972-1_13 ◽

2007 ◽

pp. 225-244

Keyword(s):

Correspondence Analysis ◽

Canonical Correspondence Analysis

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Environmental factors including management practices as correlates of weed community composition in spring seeded crops

Canadian Journal of Botany ◽

10.1139/b92-240 ◽

1992 ◽

Vol 70 (10) ◽

pp. 1931-1939 ◽

Cited By ~ 58

Author(s):

M. R. T. Dale ◽

A. G. Thomas ◽

E. A. John

Keyword(s):

Community Composition ◽

Correspondence Analysis ◽

Canonical Correspondence Analysis ◽

Environmental Variables ◽

Management Practices ◽

Geographic Region ◽

Climatic Conditions ◽

Weed Species ◽

Weed Communities ◽

Seed Crops

The relationship between the weed communities and environmental variables such as soil, crop, and management was investigated in two provinces of Canada. The frequencies of weed species were recorded in cereal and oilseed fields in Saskatchewan (2244 fields over 4 years) and Manitoba (864 fields over 3 years), Canada. Information on some of the physical characteristics of the fields and on the management practices imposed on them was also collected, in part by questionnaire. The two kinds of data were ordinated together using canonical correspondence analysis to assess the relationships between the species and the environmental variables. In the Saskatchewan data, there was a clear and consistent separation of the species into groups along an axis correlated with soil zone and the associated climatic gradient. In the Manitoba data, the same groups of species did not separate as clearly or as consistently, although geographic region was a major determinant of the weed communities. The greater variability may be due to the fact that the gradient of soil types is much more restricted in Manitoba and not as closely correlated with climatic conditions. In both provinces, culture practices were less important as correlates of the weed community composition than soil type or, to a certain extent, the previous crop. Key words: canonical correspondence analysis, cereal crops, oil seed crops.

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