scholarly journals Establishment of a Mouse Macula Densa Cell Line with an nNOS Promoter Driving EGFP Expression

2005 ◽  
Vol 55 (6) ◽  
pp. 365-372 ◽  
Author(s):  
Yukiko Yasuoka ◽  
Hideaki Kawada ◽  
Yoshiro Suzuki ◽  
Masahiro Sato ◽  
Hitoshi Endou ◽  
...  
Keyword(s):  
Cell Line ◽  
Macula Densa ◽  
Egfp Expression

Renal localization and regulation of 15-hydroxyprostaglandin dehydrogenase

2008 ◽  
Vol 294 (2) ◽  
pp. F433-F439 ◽  
Author(s):  
Bing Yao ◽  
Jie Xu ◽  
Raymond C. Harris ◽  
Ming-Zhi Zhang
Keyword(s):  
Proximal Tubule ◽  
Cell Line ◽  
Collecting Duct ◽  
Macula Densa ◽  
Immunofluorescent Staining ◽  
Kidney Cortex ◽  
Proximal Tubule Cell ◽  
Thick Ascending Limb ◽  
Key Enzyme ◽  
Cox 2

Tissue prostaglandin levels are determined by both biosynthesis and catabolism. The current studies report the expression and localization of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), a key enzyme in prostaglandin catabolism in the kidneys. We also investigated potential interactions between 15-PGDH and cyclooxygenase (COX), a key enzyme in prostaglandin biosynthesis. Both 15-PGDH mRNA and protein levels were significantly higher in kidney cortex than in papilla, which is opposite to the expression pattern of COX-2. In situ hybridization indicated that 15-PGDH mRNA was mainly localized to the tubular epithelial cells in kidney cortex and outer medulla but not in the glomerulus or papilla. Dual immunofluorescent staining indicated that 15-PGDH was expressed in the proximal tubule, cortical, and outer medullary thick ascending limb and collecting duct but not in the macula densa or papilla. 15-PGDH levels were significantly lower in a macula densa cell line (MMDD1) than in a proximal tubule cell line. Although a high-salt diet decreased COX-2 expression in macula densa, it increased macula densa 15-PGDH expression in both mouse and rat kidneys. In MMDD1 cells, a COX-2 inhibitor increased 15-PGDH, whereas a COX-1 inhibitor had no effect. Furthermore, intense 15-PGDH immunofluorescent staining was found in both macula densa and glomerulus in COX-2 knockout mice. The intrarenal distribution of 15-PGDH and its interactions with COX-2 suggest that differential regulation of COX-2 and 15-PGDH may play an important role in determining levels of prostaglandins involved in regulation of salt, volume, and blood pressure homeostasis.

Low salt concentrations activate AMP-activated protein kinase in mouse macula densa cells

2009 ◽  
Vol 296 (4) ◽  
pp. F801-F809 ◽  
Author(s):  
Natasha Cook ◽  
Scott A. Fraser ◽  
Marina Katerelos ◽  
Frosa Katsis ◽  
Kurt Gleich ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Line ◽  
Kinase Inhibitor ◽  
Chloride Concentration ◽  
Macula Densa ◽  
Tubuloglomerular Feedback ◽  
Specific Substrate ◽  
Ampk Activation ◽  
Low Salt ◽  
Amp Activated Protein Kinase

The energy-sensing kinase AMP-activated protein kinase (AMPK) is associated with the sodium-potassium-chloride cotransporter NKCC2 in the kidney and phosphorylates it on a regulatory site in vitro. To identify a potential role for AMPK in salt sensing at the macula densa, we have used the murine macula densa cell line MMDD1. In this cell line, AMPK was rapidly activated by isosmolar low-salt conditions. In contrast to the known salt-sensing pathway in the macula densa, AMPK activation occurred in the presence of either low sodium or low chloride and was unaffected by inhibition of NKCC2 with bumetanide. Assays using recombinant AMPK demonstrated activation of an upstream kinase by isosmolar low salt. The specific calcium/calmodulin-dependent kinase kinase inhibitor STO-609 failed to suppress AMPK activation, suggesting that it was not part of the signal pathway. AMPK activation was associated with increased phosphorylation of the specific substrate acetyl-CoA carboxylase (ACC) at Ser79, as well as increased NKCC2 phosphorylation at Ser126. AMPK activation due to low salt concentrations was inhibited by an adenovirus construct encoding a kinase dead mutant of AMPK, leading to reduced ACC Ser79 and NKCC2 Ser126 phosphorylation. This work demonstrates that AMPK activation in macula densa-like cells occurs via isosmolar changes in sodium or chloride concentration, leading to phosphorylation of ACC and NKCC2. Phosphorylation of these substrates in vivo is predicted to increase intracellular chloride and so reduce the effect of salt restriction on tubuloglomerular feedback and renin secretion.

scholarly journals Low Chloride Stimulation of Prostaglandin E2Release and Cyclooxygenase-2 Expression in a Mouse Macula Densa Cell Line

2000 ◽  
Vol 275 (48) ◽  
pp. 37922-37929 ◽  
Author(s):  
Tianxin Yang ◽  
John M. Park ◽  
Lois Arend ◽  
Yuning Huang ◽  
Rezan Topaloglu ◽  
...  
Keyword(s):  
Cell Line ◽  
Cyclooxygenase 2 ◽  
Macula Densa ◽  
Stimulation Of

Increasing NaCl Induces Superoxide Production in Macula Densa Cell Line

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Ruisheng Liu ◽  
Chunxia Lin ◽  
Oscar A. Carretero ◽  
YiLin Ren ◽  
Pamela Harding
Keyword(s):  
Cell Line ◽  
Macula Densa ◽  
Superoxide Production

scholarly journals Novel miRNA Sponges to Specifically Modulate Gene Expression in Colon Cancer Cells

2019 ◽  
Author(s):  
Alejandro Aguilera ◽  
Gloria Perazzoli ◽  
Laura Cabeza ◽  
Cristina Mesa ◽  
Francisco Quiñonero ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Fluorescent Protein ◽  
Vector System ◽  
Egfp Expression ◽  
Expression Levels ◽  
Selective Blockade ◽  
Novel Mirna ◽  
Mirna Sponges ◽  
Green Fluorescent

miRNA sponges allow the selective blockade of a complete family of associated miRNAs which induce posttranscriptional gene silencing in its target through binding to 3´UTR mRNA. MiRNA-365 and miRNA-145 are down-regulated in colorectal cancer (CRC), but not in health tissues. Based on this, we constructed two vectors by inserting miRNA sponge (one for miRNA-365 and other for miRNA-145), and used EGFP (enhanced green fluorescent protein) as a 3′ UTR reporter gene to analyse the ability of each sponge to catch its respective miRNA. qPCR results corroborated that the expression levels of both miRNAs were lower in CRC cell lines than in normal colon cell line. Flow cytometry analysis revealed a decrease of the EGFP expression levels in the cell lines transfected with both sponges, being higher on the normal cell line while CRC cell lines presented a minimal decline. Also, this decrease was inversely proportional to the levels of expression of both miRNAs obtained by qPCR. These results were corroborated by fluorescence microscopy, showing a similar decrease fluorescence. We propose a new vector system to carry in a specific way the expression of genes to CRC cells without affecting healthy cells, preventing damage to healthy tissues.

Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line

2012 ◽  
Vol 49 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Ramakant Kaushik ◽  
Karn Pratap Singh ◽  
Archana Kumari ◽  
Manoj Kumar Singh ◽  
Radhey Shyam Manik ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Bubalus Bubalis ◽  
Egfp Expression ◽  
Epithelial Cell Line ◽  
Mammary Gland Epithelial Cell

Ultrastructure of NPC/HK1 cells heterotransplanted in athymic nude mice

1982 ◽  
Vol 40 ◽  
pp. 236-237
Author(s):  
E.C. Chew ◽  
C.L. Li ◽  
D.P. Huang ◽  
H.C. Ho ◽  
L.S. Mak ◽  
...  
Keyword(s):  
Cell Line ◽  
Nude Mice ◽  
Biopsy Specimen ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Recurrent Tumour ◽  
Athymic Nude Mice ◽  
Cell Line Establishment ◽  
Fine Structures ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been established from a biopsy specimen of a recurrent tumour of the nasopharynx which was histologically diagnosed as a moderately to well differentiated squamous cell carcinoma. A definite decrease in the amount of tonofilaments and desmosomes in the NPC/HK1 cells during the cell line establishment was observed. The present communication reports on the fine structures of the NPC/HK1 cells heterotraneplanted in athymic nude mice.

Ultrastructure of Choriocarcinoma in Vitro

1969 ◽  
Vol 27 ◽  
pp. 362-363
Author(s):  
John C. Garancis ◽  
R. A. Pattillo
Keyword(s):  
Cell Line ◽  
Physiological Function ◽  
Cell System ◽  
Bewo Cell ◽  
Bewo Cells ◽  
Gestational Choriocarcinoma ◽  
Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

Sign in / Sign up

Export Citation Format

Share Document