scholarly journals Screening of Salmonella spp. and Chlamydophila psittaciin parrots domiciled in Rio Branco, Acre, Brazil

2021 ◽  
Vol 15 (4) ◽  
pp. 339-344
Author(s):  
Breno Kalyl Freitas Nascimento ◽  
Luciana dos Santos Medeiros ◽  
Leandro dos Santos Machado ◽  
Vânia Maria França Ribeiro
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Infectious Diseases ◽  
Emerging Infectious Diseases ◽  
Wild Animals ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Zoonotic Pathogens ◽  
Polymerase Chain

A large proportion of emerging infectious diseases (60.3%) globally are zoonotic pathogens, and of these, 71.8% originate from wild animals. Salmonellosis and psittacosis, diseases caused by Salmonella spp. and Chlamydophila psittaci, respectively, in wild animals are zoonoses with great risks to public health. Therefore, this study aimed to investigate the presence of Salmonella spp. and C. psittaci in parrots domiciled in Rio Branco, Acre. The animals in the study were raised as pets, and selection was performed based on convenience criteria. The birds were manually restrained to collect biological materials. Subsequently, conventional microbiological and biochemical tests were performed to identify Salmonella spp., and polymerase chain reaction analyses were conducted to identify C. psittaci and Salmonella spp. It was not possible to isolate Salmonella spp. and C. psittaci in the sampled birds. However, the presence of these bacteria in parrots cannot be ruled out because intermittent release and diagnostic limitations are widely described in the literature.

scholarly journals Detection and Molecular Characterization of Vibrio Parahaemolyticus in Shrimp Samples

2018 ◽  
Vol 12 (1) ◽  
pp. 46-50 ◽  
Author(s):  
Daryoush Asgarpoor ◽  
Fakhri Haghi ◽  
Habib Zeighami
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Vibrio Parahaemolyticus ◽  
Foodborne Diseases ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Global Issue ◽  
Retail Outlets ◽  
Polymerase Chain

Background:Food safety has emerged as an important global issue with international trade and public health implications. Bacterial pathogens asVibrio parahaemolyticusrecognized as an important cause of foodborne diseases related to the consumption of raw, undercooked or mishandled seafood worldwide.Methods:A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains ofV. parahaemolyticus.The shrimp samples were immediately homogenized and cultured on TCBS agarand subjected to confirmatory biochemical tests. Polymerase Chain Reaction (PCR) was performed for detection of total and pathogenicV. parahaemolyticusby amplification ofvp–toxR,tdhandtrhgenes.Results:The conventional method indicated that 16 (22.8%) of samples were positive forV. parahaemolyticus. However, PCR verified that only 12 (17.1%) shrimp samples were positive forV. parahaemolyticus.Of the 70 shrimp samples in our study, only 2 (2.8%)tdhand 1 (1.4%)trhpositive strains were identified.Conclusion:Detection oftdhand/ ortrhpositiveV. parahaemolyticusin shrimp marketed in Zanjan, Iran shows a probable risk for public health. Therefore, the reliable molecular methods for monitoring of potentially pathogenicV. parahaemolyticusare strongly recommended for the routine seafood examination.

scholarly journals Pathology of Fowl Paratyphoid and Molecular Detection of Its Pathogen in Layer Chickens

2021 ◽  
Vol 24 (2) ◽  
pp. 47-57
Author(s):  
J Alam ◽  
T Chakma ◽  
MS Islam ◽  
MT Islam ◽  
MAHNA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Salmonella Enteritidis ◽  
Molecular Detection ◽  
Inflammatory Cells ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Gross Lesions ◽  
Pcr Method ◽  
Polymerase Chain

The study was aimed to ascertain the pathology of fowl paratyphoid and molecular detection of its causal agent (Salmonella spp) in chickens. Pathological and swab samples were collected from layers in Gazipur district, Bangladesh. For observing the gross and microscopic lesions of different organs necropsy and histopathology were done, and to isolate and identify the Salmonella spp, different bacteriological tests and Polymerase Chain Reaction (PCR) were performed. Swabs from 150 chickens showed 66% of salmonellosis. Gram’s staining of isolated bacteria showed pink colored rod shaped bacilli. In biochemical tests, Salmonella fermented dextrose, maltose, xylose, arabinose, dulcitol, mannitol except lactose and sucrose. Investigation of gross lesions at necropsy revealed hemorrhage and congestion in intestine, liver, spleen and ovaries. Necrotic foci were found in liver and spleen, and button like ulceration in cecal tonsils as well. Microscopic lesions included hemorrhage and focal necrosis in liver and spleen. Congestion and infiltrations of inflammatory cells were observed in small intestine. Ovary was hemorrhagic and there was infiltration of heterophils. Biochemically positive and isolated Salmonella organisms were confirmed by PCR method using invA and IE1 primers. The final results showed that a total of 91.7% Salmonella suspected cultures were confirmed as Salmonella Enteritidis. Ann. Bangladesh Agric. (2020) 24(2) : 47-57

scholarly journals Development of a Genoserotyping Method for Salmonella Infantis Detection on the Basis of Pangenome Analysis

2020 ◽  
Vol 9 (1) ◽  
pp. 67
Author(s):  
Seung-Min Yang ◽  
Jiwon Baek ◽  
Eiseul Kim ◽  
Hyeon-Be Kim ◽  
Seyoung Ko ◽  
...  
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Bacterial Species ◽  
Cost Effective ◽  
The Other ◽  
Gene Marker ◽  
Diagnostic Assay ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Substantial Economic Burden

In recent years, Salmonella Infantis has become a predominant serovariant in clinical and poultry isolates, thereby imposing a substantial economic burden on both public health and the livestock industry. With the aim of coping with the steep increase in serovar Infantis prevalence, a polymerase chain reaction (PCR)-based rapid and accurate diagnostic assay was developed in this study through pangenome profiling of 60 Salmonella serovars. A gene marker, SIN_02055, was identified, which is present in the S. Infantis genome but not in the pangenome of the other serovars. Primers specific to SIN_02055 were used to accurately detect serovar Infantis, and to successfully differentiate Infantis from the other 59 serovars in real-time PCR with a R2 of 0.999 and an efficiency of 95.76%. The developed method was applied to 54 Salmonella strains belonging to eight dominant serovars, and distinguished Infantis from the other seven serovars with an accuracy of 100%. The diagnostic primer set also did not show false positive amplification with 32 strains from eight non-Salmonella bacterial species. This cost-effective and rapid method can be considered an alternative to the classic serotyping using antisera.

Polymerase Chain Reaction Detection of Salmonella spp. in Fecal Samples of Pet Birds

2008 ◽  
Vol 3 (1) ◽  
pp. e29-e29
Author(s):  
B. Sareyyüpoğlu ◽  
A Çelik Ok ◽  
Z. Cantekin ◽  
H. Yardimci ◽  
M. Akan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Pet Birds ◽  
Polymerase Chain

scholarly journals Prescription Surveillance and Polymerase Chain Reaction Testing to Identify Pathogens during Outbreaks of Infection

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Hiroaki Sugiura ◽  
Tsuguto Fujimoto ◽  
Tamie Sugawara ◽  
Nozomu Hanaoka ◽  
Masami Konagaya ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Syndromic Surveillance ◽  
Respiratory Infections ◽  
Emerging Infectious Diseases ◽  
Upper Respiratory Tract ◽  
Chain Reaction ◽  
Specific Pathogen ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Tract Infections

Syndromic surveillance, including prescription surveillance, offers a rapid method for the early detection of agents of bioterrorism and emerging infectious diseases. However, it has the disadvantage of not considering definitive diagnoses. Here, we attempted to definitively diagnose pathogens using polymerase chain reaction (PCR) immediately after the prescription surveillance system detected an outbreak. Specimens were collected from 50 patients with respiratory infections. PCR was used to identify the pathogens, which included 14 types of common respiratory viruses andMycoplasma pneumoniae. Infectious agents includingM. pneumoniae, respiratory syncytial virus (RSV), rhinovirus, enterovirus, and parainfluenza virus were detected in 54% of patients. For the rapid RSV diagnosis kit, sensitivity was 80% and specificity was 85%. For the rapid adenovirus diagnosis kit, no positive results were obtained; therefore, sensitivity could not be calculated and specificity was 100%. Many patients were found to be treated for upper respiratory tract infections without the diagnosis of a specific pathogen. In Japan, an outbreak ofM. pneumoniaeinfection began in 2011, and our results suggested that this outbreak may have included false-positive cases. By combining syndromic surveillance and PCR, we were able to rapidly and accurately identify causative pathogens during a recent respiratory infection outbreak.

An Investigation of Contagious Agalactia Disease of Sheep and Goats in Isparta and Afyonkarahisar in Turkey

2021 ◽  
Author(s):  
Ülkü Karatekeli ◽  
Beytullah Kenar
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Inhibition ◽  
Colony Morphology ◽  
Economic Losses ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Contagious Agalactia ◽  
Nasal Swabs ◽  
Polymerase Chain

Background: Contagious agalactia causes significant economic losses. The aim of this study is to investigate the presence of contagious agalactia disease in cities of Isparta and Afyonkarahisar, Turkey. Methods: The study includes 45.500 animals in 220 ovine enterprises and samples were taken from those suspected of contagious agalactia disease. 202 animals in the 21 ovine enterprises comprised of 139 goats, 56 sheep, 3 kid goats, 2 goats, 2 lambs in total suspected of the disease were sampled. A total of 289 samples were collected, including 91 milk samples, 28 nasal swabs, 101 eye swabs, 8 joint fluids and 61 ear swabs. The isolates obtained after incubation were identified with polymerase chain reaction by using specific primers to assess film and spot formation, glucose fermentation, growth inhibition tests. Result: Three Mycoplasma spp. isolates obtained from 28 nasal swabs turned out to be negative for M. agalactiae after PCR analysis. Colony morphology, biochemical tests and growth inhibition tests revealed that one agent was M. arginine and the two factors were identified as M. ovipneumoniae with centerless colony morphology. The obtained results were confirmed with the polymerase chain reaction. None of the four factors causing contagious agalactia were isolated and identified.

scholarly journals Validation of Droplet Digital Polymerase Chain Reaction for Salmonella spp. Quantification

2020 ◽  
Vol 11 ◽  
Author(s):  
Carolina Villamil ◽  
Martha Nancy Calderon ◽  
Maria Mercedes Arias ◽  
John Emerson Leguizamon
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction
Sign in / Sign up

Export Citation Format

Share Document