Controlled Bioactive Molecules Delivery Strategies for Tendon and Ligament Tissue Engineering using Polymeric Nanofibers

2015 ◽  
Vol 21 (15) ◽  
pp. 1991-2005 ◽  
Author(s):  
Thomas Teh ◽  
James Goh ◽  
Siew Toh
Keyword(s):  
Tissue Engineering ◽  
Bioactive Molecules ◽  
Polymeric Nanofibers ◽  
Delivery Strategies ◽  
Ligament Tissue Engineering

scholarly journals FUNCTIONALIZATION OF POLYMERIC NANOFIBERS USING PLATELETS FOR MELANOCYTE CULTURE

2020 ◽  
Vol 50 (1) ◽  
pp. 16-22
Author(s):  
Karolina Vocetkova ◽  
Vera Sovkova ◽  
Matej Buzgo ◽  
Radek Divin ◽  
Evzen Amler ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Scanning Electron Microscopy ◽  
Wide Spectrum ◽  
Bioactive Molecules ◽  
Interdisciplinary Field ◽  
Polymeric Nanofibers ◽  
Psychosocial Implications ◽  
Mts Assay ◽  
Scanning Electron

Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the therapy of multiple diseases, including depigmentation disorders. The most common depigmentation disorder is vitiligo, a disease with deep psychosocial implications. Thanks to their unique properties, electrospun polymeric nanofibers represent a material suitable for tissue engineering applications. Furthermore, they may be functionalized with platelets, cells that contain a wide spectrum of growth factors and chemokines. The aim of this paper was to evaluate the functionalization of polymeric nanofibers with platelets and their effects in melanocyte culture. The scaffolds were visualized using scanning electron microscopy, the metabolic activity and proliferation of melanocytes was determined using MTS assay and dsDNA quantification, respectively. Furthermore, the melanocytes were stained and visualized using confocal microscopy. The acquired data showed that poly-ε-caprolactone functionalized with platelets promoted the viability and proliferation of melanocytes. According to the results, such a functionalized scaffold combining nanofibers and platelets may be suitable for melanocyte culture.

Mechano growth factor-C24E, a potential promoting biochemical factor for ligament tissue engineering

2016 ◽  
Vol 105 ◽  
pp. 249-263 ◽  
Author(s):  
Yang Song ◽  
Can Yu ◽  
Chunli Wang ◽  
Xingshuang Ma ◽  
Kang Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Mechano Growth Factor ◽  
Biochemical Factor ◽  
Ligament Tissue Engineering

scholarly journals Polymeric Bioinks for 3D Hepatic Printing

Chemistry ◽  
2021 ◽  
Vol 3 (1) ◽  
pp. 164-181
Author(s):  
Joyita Sarkar ◽  
Swapnil C. Kamble ◽  
Nilambari C. Kashikar
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Synthetic Polymers ◽  
Bioactive Molecules ◽  
Complex Geometries ◽  
Printing Techniques ◽  
Natural And Synthetic

Three-dimensional (3D) printing techniques have revolutionized the field of tissue engineering. This is especially favorable to construct intricate tissues such as liver, as 3D printing allows for the precise delivery of biomaterials, cells and bioactive molecules in complex geometries. Bioinks made of polymers, of both natural and synthetic origin, have been very beneficial to printing soft tissues such as liver. Using polymeric bioinks, 3D hepatic structures are printed with or without cells and biomolecules, and have been used for different tissue engineering applications. In this review, with the introduction to basic 3D printing techniques, we discuss different natural and synthetic polymers including decellularized matrices that have been employed for the 3D bioprinting of hepatic structures. Finally, we focus on recent advances in polymeric bioinks for 3D hepatic printing and their applications. The studies indicate that much work has been devoted to improvising the design, stability and longevity of the printed structures. Others focus on the printing of tissue engineered hepatic structures for applications in drug screening, regenerative medicine and disease models. More attention must now be diverted to developing personalized structures and stem cell differentiation to hepatic lineage.

scholarly journals Marine Polysaccharides: A Source of Bioactive Molecules for Cell Therapy and Tissue Engineering

Marine Drugs ◽  
2011 ◽  
Vol 9 (9) ◽  
pp. 1664-1681 ◽  
Author(s):  
Karim Senni ◽  
Jessica Pereira ◽  
Farida Gueniche ◽  
Christine Delbarre-Ladrat ◽  
Corinne Sinquin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Therapy ◽  
Bioactive Molecules

scholarly journals Tissue engineering of urethra: Systematic review of recent literature

2017 ◽  
Vol 242 (18) ◽  
pp. 1772-1785 ◽  
Author(s):  
Stanislav Žiaran ◽  
Martina Galambošová ◽  
L'uboš Danišovič
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Clinical Trials ◽  
Animal Studies ◽  
Recent Literature ◽  
Cell Attachment ◽  
Experimental Studies ◽  
Clinical Results ◽  
Bioactive Molecules ◽  
Urethral Reconstruction

The purpose of this article was to perform a systematic review of the recent literature on urethral tissue engineering. A total of 31 articles describing the use of tissue engineering for urethra reconstruction were included. The obtained results were discussed in three groups: cells, scaffolds, and clinical results of urethral reconstructions using these components. Stem cells of different origin were used in many experimental studies, but only autologous urothelial cells, fibroblasts, and keratinocytes were applied in clinical trials. Natural and synthetic scaffolds were studied in the context of urethral tissue engineering. The main advantage of synthetic ones is the fact that they can be obtained in unlimited amount and modified by different techniques, but scaffolds of natural origin normally contain chemical groups and bioactive proteins which increase the cell attachment and may promote the cell proliferation and differentiation. The most promising are smart scaffolds delivering different bioactive molecules or those that can be tubularized. In two clinical trials, only onlay-fashioned transplants were used for urethral reconstruction. However, the very promising results were obtained from animal studies where tubularized scaffolds, both non-seeded and cell-seeded, were applied. Impact statement The main goal of this article was to perform a systematic review of the recent literature on urethral tissue engineering. It summarizes the most recent information about cells, seeded or non-seeded scaffolds and clinical application with respect to regeneration of urethra.

TRENDS AND CHALLENGES OF CARTILAGE TISSUE ENGINEERING

2009 ◽  
Vol 21 (03) ◽  
pp. 149-155 ◽  
Author(s):  
Hsu-Wei Fang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factors ◽  
Bone Cells ◽  
Cartilage Tissue Engineering ◽  
Bioactive Molecules ◽  
In Vivo Studies ◽  
Cartilage Tissue

Cartilage injuries may be caused by trauma, biomechanical imbalance, or degenerative changes of joint. Unfortunately, cartilage has limited capability to spontaneous repair once damaged and may lead to progressive damage and degeneration. Cartilage tissue-engineering techniques have emerged as the potential clinical strategies. An ideal tissue-engineering approach to cartilage repair should offer good integration into both the host cartilage and the subchondral bone. Cells, scaffolds, and growth factors make up the tissue engineering triad. One of the major challenges for cartilage tissue engineering is cell source and cell numbers. Due to the limitations of proliferation for mature chondrocytes, current studies have alternated to use stem cells as a potential source. In the recent years, a lot of novel biomaterials has been continuously developed and investigated in various in vitro and in vivo studies for cartilage tissue engineering. Moreover, stimulatory factors such as bioactive molecules have been explored to induce or enhance cartilage formation. Growth factors and other additives could be added into culture media in vitro, transferred into cells, or incorporated into scaffolds for in vivo delivery to promote cellular differentiation and tissue regeneration.Based on the current development of cartilage tissue engineering, there exist challenges to overcome. How to manipulate the interactions between cells, scaffold, and signals to achieve the moderation of implanted composite differentiate into moderate stem cells to differentiate into hyaline cartilage to perform the optimum physiological and biomechanical functions without negative side effects remains the target to pursue.

scholarly journals Targeted Delivery of Bioactive Molecules for Vascular Intervention and Tissue Engineering

2018 ◽  
Vol 9 ◽  
Author(s):  
Hannah A. Strobel ◽  
Elisabet I. Qendro ◽  
Eben Alsberg ◽  
Marsha W. Rolle
Keyword(s):  
Tissue Engineering ◽  
Targeted Delivery ◽  
Bioactive Molecules ◽  
Vascular Intervention
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