Relative In Vitro Potentials of Parthenolide to Induce Apoptosis and Cell Cycle Arrest in Skin Cancer Cells

From a root bark of Lespedeza bicolor Turch we isolated two new (7 and 8) and six previously known compounds (1–6) belonging to the group of prenylated polyphenols. Their structures were elucidated using mass spectrometry, nuclear magnetic resonance and circular dichroism spectroscopy. These natural compounds selectively inhibited human drug-resistant prostate cancer in vitro. Prenylated pterocarpans 1–3 prevented the cell cycle progression of human cancer cells in S-phase. This was accompanied by a reduced expression of mRNA corresponding to several human cyclin-dependent kinases (CDKs). In contrast, compounds 4–8 induced a G1-phase cell cycle arrest without any pronounced effect on CDKs mRNA expression. Interestingly, a non-substituted hydroxy group at C-8 of ring D of the pterocarpan skeleton of compounds 1–3 seems to be important for the CDKs inhibitory activity.

Download Full-text

Cell cycle arrest and induction of apoptosis in pancreatic cancer cells exposed to eicosapentaenoic acid in vitro

British Journal of Cancer ◽

10.1038/bjc.1996.552 ◽

1996 ◽

Vol 74 (9) ◽

pp. 1375-1383 ◽

Cited By ~ 65

Author(s):

PBS Lai ◽

JA Ross ◽

KCH Fearon ◽

JD Anderson ◽

DC Carter

Keyword(s):

Cell Cycle ◽

Pancreatic Cancer ◽

Eicosapentaenoic Acid ◽

Cell Cycle Arrest ◽

Cancer Cells ◽

Cycle Arrest ◽

Pancreatic Cancer Cells ◽

Induction Of Apoptosis

Download Full-text

Cell cycle arrest and the induction of apoptosis in pancreatic cancer cells exposed to adenosine triphosphate in vitro

Oncology Reports ◽

10.3892/or.9.1.113 ◽

2002 ◽

Cited By ~ 1

Author(s):

Takayuki Yamada ◽

Fumikazu Okajima ◽

Mohammed Akbar ◽

Hideaki Tomura ◽

T. Narita ◽

...

Keyword(s):

Cell Cycle ◽

Pancreatic Cancer ◽

Cell Cycle Arrest ◽

Cancer Cells ◽

Adenosine Triphosphate ◽

Cycle Arrest ◽

Pancreatic Cancer Cells ◽

Induction Of Apoptosis

Download Full-text

Cepharanthine Induces Autophagy, Apoptosis and Cell Cycle Arrest in Breast Cancer Cells

Cellular Physiology and Biochemistry ◽

10.1159/000471234 ◽

2017 ◽

Vol 41 (4) ◽

pp. 1633-1648 ◽

Cited By ~ 24

Author(s):

Sumei Gao ◽

Xiaoyan Li ◽

Xia Ding ◽

Wenwen Qi ◽

Qifeng Yang

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Cell Cycle Arrest ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Invasion And Migration ◽

Cycle Arrest ◽

And Migration ◽

Anti Tumour Effect

Background: Cepharanthine (CEP) is a biscoclaurine alkaloid extracted from Stephania cepharantha and has been shown to have an anti-tumour effect on different types of cancers. However, the anti-cancer effect of CEP on human breast cancer cells is still unclear. Methods: We used MTT, clone formation, in vitro scratch, invasion and migration assays to confirm the inhibitory role of CEP on the proliferation of breast cancer cells. Flow cytometry, plasmid construction and western blot analysis were used to study the detailed mechanisms. Results: Our study showed that CEP could inhibit cell proliferation by inducing autophagy, apoptosis, and G0/G1 cell cycle arrest of breast cancer cells. Furthermore, we found that CEP induced autophagy and apoptosis by inhibiting the AKT/mTOR signalling pathway. Conclusion: We found that CEP could inhibit growth and motility of MCF-7 and MDA-MB-231 breast cancer cell. Our study revealed an anti-tumour effect of CEP on breast cancer cells and suggests that CEP could be a potential new clinical therapy for breast cancer.

Download Full-text

Cell-cycle arrest and senescence in TP53-wild type renal carcinoma by enhancer RNA-P53-bound enhancer regions 2 (p53BER2) in a p53-dependent pathway

Cell Death and Disease ◽

10.1038/s41419-020-03229-8 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Haibiao Xie ◽

Kaifang Ma ◽

Kenan Zhang ◽

Jingcheng Zhou ◽

Lei Li ◽

...

Keyword(s):

Cell Cycle ◽

Cell Cycle Arrest ◽

Cancer Cells ◽

Cell Lines ◽

Kidney Cancer ◽

Renal Cancer ◽

Wild Type ◽

Enhancer Rna ◽

Cycle Arrest

AbstractTP53 is a classic tumor suppressor, but its role in kidney cancer remains unclear. In our study, we tried to explain the role of p53 in kidney cancer through the p53-related enhancer RNA pathway. Functional experiments were used to explore whether P53-bound enhancer regions 2 (p53BER2) has a role in the cell cycle and senescence response of TP53-wild type (WT) renal cancer cells in vitro or vivo. RNA-sequencing was used to identify the potential target of p53BER2. The results showed that the expression level of P53BER2 was downregulated in renal cancer tissues and cell lines, further dual-luciferase experiments and APR-256-reactivated experiments showed p53BER2 expresses in a p53-dependent way. Moreover, knockdown p53BER2 could reverse nutlin-3-induced cytotoxic effect in TP53-WT cell lines. Further exploration showed the downregulation of p53BER2 could reverse nutlin-3-induced G1-arrest and senescence in TP53-WT cell lines. What is more, the knockdown of p53BER2 showed resistance to nutlin-3 treatment in vivo. Additionally, we found BRCA2 could be regulated by p53BER2 in vitro and vivo; further experiment showed p53BER2 could induce cell-cycle arrest and DNA repair by mediating BRCA2. In summary, the p53-associated enhancer RNA-p53BER2 mediates the cell cycle and senescence of p53 in TP53-WT renal cancer cells.

Download Full-text