Ionic Liquids, Microextraction Methods and Capillary Electrophoresis in Biomedical Research

2020 ◽  
Vol 17 ◽  
Author(s):  
Natalia Miękus ◽  
Martyna Ceraficka ◽  
Marta Chyła ◽  
Aleksandra Durska ◽  
Tomasz Bączek
Keyword(s):  
Ionic Liquid ◽  
Capillary Electrophoresis ◽  
Biomedical Research ◽  
Drug Targets ◽  
Biological Fluids ◽  
Tissue Samples ◽  
Analytical Strategies ◽  
Life Threatening ◽  
Speed Up ◽  
Analytical Approaches

Abstract:: The review aims to present the importance of implementing microextraction-, capillary electrophoresis- and ionic liquid-based approaches in biomedical research. These analytical strategies could improve the biochemical diagnosis of various life-threatening diseases, aid in the search for therapeutic agents and the discovery of drug targets and could be used when designing newer, safer medicinal products. All the proposed analytical approaches meet the requirements of “green chemistry”-based methods, which is relevant nowadays in view of the pollution of the Earth becoming a serious problem. The review is divided into three main sections, and biomedical examples of the application of each presented approach are discussed. It is assumed that the undoubted advantages of ionic liquid-, microextraction- and capillary electrophoresis-based methods will speed up their use in the study of various clinically important analytes from different biological fluids and tissue samples.

scholarly journals Food Allergen Detection Methods: A Coordinated Approach

2004 ◽  
Vol 87 (6) ◽  
pp. 1383-1390 ◽  
Author(s):  
Philip R Goodwin
Keyword(s):  
Hazard Analysis ◽  
Adult Population ◽  
Control Point ◽  
Cost Effective ◽  
Test Methods ◽  
Detection Methods ◽  
Critical Control Point ◽  
Life Threatening ◽  
Monitoring And Surveillance ◽  
Analytical Approaches

Abstract The levels (1–2%) and increasing severity of allergic responses to food in the adult population are well documented, as is the phenomenon of even higher (3–8%) and apparently increasing incidence in children, albeit that susceptibility decreases with age. Problematic foods include peanut, milk, eggs, tree nuts, and sesame, but the list is growing as awareness continues to rise. The amounts of such foods that can cause allergic reactions is difficult to gauge; however, the general consensus is that ingestion of low parts per million is sufficient to cause severe reactions in badly affected individuals. Symptoms can rapidly—within minutes—progress from minor discomfort to severe, even life-threatening anaphylactic shock in those worst affected. Given the combination of high incidence of atopy, potential severity of response, and apparently widespread instances of “hidden” allergens in the food supply, it is not surprising that this issue is increasingly subject to legislative and regulatory scrutiny. In order to assist in the control of allergen levels in foods to acceptable levels, analysts require a combination of test methods, each designed to produce accurate, timely, and cost-effective analytical information. Such information contributes significantly to Hazard Analysis Critical Control Point programs to determine food manufacturers’ risk and improves the accuracy of monitoring and surveillance by food industry, commercial, and enforcement laboratories. Analysis thereby facilitates improvements in compliance with labeling laws with concomitant reductions in risks to atopic consumers. This article describes a combination of analytical approaches to fulfill the various needs of these 3 analytical communities.

Laboratory Specimens and Genetic Privacy: Evolution of Legal Theory

2013 ◽  
Vol 41 (S1) ◽  
pp. 65-68 ◽  
Author(s):  
Michelle Huckaby Lewis
Keyword(s):  
Biomedical Research ◽  
Biological Samples ◽  
Biological Tissue ◽  
Genetic Information ◽  
Legal Theory ◽  
Genetic Privacy ◽  
Tissue Samples

Human biological tissue samples are an invaluable resource for biomedical research designed to find causes of diseases and their treatments. Controversy has arisen, however, when research has been conducted with laboratory specimens either without the consent of the source of the specimen or when the research conducted with the specimen has expanded beyond the scope of the original consent agreement. Moreover, disputes have arisen regarding which party, the researcher or the source of the specimen, has control over who may use the specimens and for what purposes. The purposes of this article are: (1) to summarize the most important litigation regarding the use of laboratory specimens, and (2) to demonstrate how legal theory regarding control of laboratory specimens has evolved from arguments based upon property interests in biological samples to claims that the origins of laboratory specimens have privacy interests in their genetic information that should be protected.

Analysis of nitrite and nitrate in biological fluids by capillary electrophoresis

1999 ◽  
Vol 32 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Phuong Nhi Bories ◽  
Elisabeth Scherman ◽  
Lydie Dziedzic
Keyword(s):  
Capillary Electrophoresis ◽  
Biological Fluids

scholarly journals Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6694
Author(s):  
Niamat Khan ◽  
Sidra Shahid ◽  
Abdul R. Asif
Keyword(s):  
Drug Targets ◽  
Protein Complexes ◽  
Dynamic Structure ◽  
Associated Diseases ◽  
Specific Complex ◽  
Analytical Strategies ◽  
Associated Proteins ◽  
Therapeutic Molecules ◽  
Unique Nature ◽  
Mass Spectrometric Identification

Chromatin is a dynamic structure comprising of DNA and proteins. Its unique nature not only help to pack the DNA tightly within the cell but also is pivotal in regulating gene expression DNA replication. Furthermore it also protects the DNA from being damaged. Various proteins are involved in making a specific complex within a chromatin and the knowledge about these interacting partners is helpful to enhance our understanding about the pathophysiology of various chromatin associated diseases. Moreover, it could also help us to identify new drug targets and design more effective remedies. Due to the existence of chromatin in different forms under various physiological conditions it is hard to develop a single strategy to study chromatin associated proteins under all conditions. In our current review, we tried to provide an overview and comparative analysis of the strategies currently adopted to capture the DNA bounded protein complexes and their mass spectrometric identification and quantification. Precise information about the protein partners and their function in the DNA-protein complexes is crucial to design new and more effective therapeutic molecules against chromatin associated diseases.

Analysis of aromatic acids by nonaqueous capillary electrophoresis with ionic-liquid electrolytes

2014 ◽  
Vol 35 (23) ◽  
pp. 3310-3316 ◽  
Author(s):  
Yuanqi Lu ◽  
Dunqing Wang ◽  
Chunyan Kong ◽  
Hao Zhong ◽  
Michael C. Breadmore
Keyword(s):  
Ionic Liquid ◽  
Capillary Electrophoresis ◽  
Aromatic Acids ◽  
Nonaqueous Capillary Electrophoresis ◽  
Liquid Electrolytes
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