Spectrophotometric Techniques: A Versatile Tool for Bioprocess Monitoring

2020 ◽  
Vol 09 ◽  
Author(s):  
Chandni Chandarana ◽  
Jyoti Suthar ◽  
Aman Goyel
Keyword(s):  
Raman Spectroscopy ◽  
Online Monitoring ◽  
Uv Spectroscopy ◽  
Continuous Process ◽  
Review Article ◽  
Bioprocess Monitoring ◽  
Spectroscopic Methods ◽  
Sample Analysis ◽  
Online Analysis ◽  
Versatile Tool

Abstract:: Online analysis of bioprocesses by analytical spectroscopic methods is used to produce fast sample analysis. Bio-transformations are directly controlled by continuous process. It improves management of quality. Various methods for online analysis have been reported. This review article majorly covers applications for infrared (NIR and MIR); Fluorescence; Ultraviolet (UV) Spectroscopy and Raman Spectroscopy for online monitoring of bioprocesses.

scholarly journals Raman Spectrometry as a Tool for an Online Control of a Phototrophic Biological Nutrient Removal Process

2021 ◽  
Vol 11 (14) ◽  
pp. 6600
Author(s):  
Rita D. G. Franca ◽  
Virgínia C. F. Carvalho ◽  
Joana C. Fradinho ◽  
Maria A. M. Reis ◽  
Nídia D. Lourenço
Keyword(s):  
Raman Spectroscopy ◽  
Nutrient Removal ◽  
Latent Variables ◽  
Cross Validation ◽  
Online Monitoring ◽  
Biological Nutrient Removal ◽  
Raman Shift ◽  
Bioprocess Monitoring ◽  
Efficient Operation ◽  
Total P

Real-time bioprocess monitoring is crucial for efficient operation and effective bioprocess control. Aiming to develop an online monitoring strategy for facilitating optimization, fault detection and decision-making during wastewater treatment in a photo-biological nutrient removal (photo-BNR) process, this study investigated the application of Raman spectroscopy for the quantification of total organic content (TOC), volatile fatty acids (VFAs), carbon dioxide (CO2), ammonia (NH3), nitrate (NO3), phosphate (PO4), total phosphorus (total P), polyhydroxyalkanoates (PHAs), total carbohydrates, total and volatile suspended solids (TSSs and VSSs, respectively). Specifically, partial least squares (PLS) regression models were developed to predict these parameters based on Raman spectra, and evaluated based on a full cross-validation. Through the optimization of spectral pre-processing, Raman shift regions and latent variables, 8 out of the 11 parameters that were investigated—namely TOC, VFAs, CO2, NO3, total P, PHAs, TSSs and VSSs—could be predicted with good quality by the respective Raman-based PLS calibration models, as shown by the high coefficient of determination (R2 > 90.0%) and residual prediction deviation (RPD > 5.0), and relatively low root mean square error of cross-validation. This study showed for the first time the high potential of Raman spectroscopy for the online monitoring of TOC, VFAs, CO2, NO3, total P, PHAs, TSSs and VSSs in a photo-BNR reactor.

scholarly journals Vitamin C − protective role in oxidative stress conditions induced in human normal colon cells by label free Raman spectroscopy and imaging

2021 ◽  
Author(s):  
Karolina Beton ◽  
Beata Brozek-Pluska
Keyword(s):  
Oxidative Stress ◽  
Raman Spectroscopy ◽  
Vitamin C ◽  
Human Colon ◽  
Protective Role ◽  
Spectroscopic Methods ◽  
Label Free ◽  
Normal Cells ◽  
Colon Cells ◽  
Cancerous Cells

Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer, can detect it in advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise and unambiguous differentiation of healthy and cancerous samples. the most important advantage of Raman spectroscopy is ability to identify biomarkers that help in differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for: lipids, proteins, DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS by spectroscopic methods. Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal, ROS injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis.

Ensuring hemodialysis adequacy by dialysis dose monitoring with UV spectroscopy analysis of spent dialyzate

2021 ◽  
pp. 039139882110598
Author(s):  
Li Zhang ◽  
Wenhu Liu ◽  
Chuanming Hao ◽  
Yani He ◽  
Ye Tao ◽  
...  
Keyword(s):  
Online Monitoring ◽  
Uv Spectroscopy ◽  
Daily Practice ◽  
Dialysis Dose ◽  
Dialysis Adequacy ◽  
Av Fistula ◽  
Dose Monitoring ◽  
Significant Step

Introduction: Patients’ session-to-session variation has been shown to influence outcomes, making critical the monitoring of dialysis dose in each session. The aim of this study was to detect the intra-patient variability of blood single pool Kt/V as measured from pre-post dialysis blood urea and from the online tool Adimea®, which measures the ultraviolet absorbance of spent dialyzate. Methods: This open, one-armed, prospective non-interventional study, evaluates patients on bicarbonate hemodialysis or/and on hemodiafiltration. Dialysis was performed with B. Braun Dialog+ machines equipped with Adimea®. In the course of the prospective observation, online monitoring with Adimea® in each session was established without the target warning function being activated. A sample size of 97 patients was estimated. Results: A total of 120 patients were enrolled in six centers in China (mean age 51.5 ± 12.2 years, 86.7% males, 24.2% diabetics). All had an AV-fistula. The proportion of patients with blood Kt/V < 1.20 at baseline was 48.3%. During follow-up with Adimea®, the subgroup with Kt/V > 1.20 at baseline remains at the same adequacy level for more than 90% of the patients. Those with a Kt/V < 1.20 at baseline, showed a significant increase of Kt/V to 60% of the patients reaching the adequacy level >1.20. The coefficient of variation for spKt/V as evaluated by Adimea® was 9.6 ± 3.4%, not significantly different from the 9.6 ± 8.6% as blood Kt/V taken at the same time. Conclusion: Online monitoring of dialysis dose by Adimea® improves and maintains dialysis adequacy. Implementing online monitoring by Adimea into daily practice moves the quality of dialysis patient care a significant step forward.

Toward the Development of Raman Spectroscopy as a Nonperturbative Online Monitoring Tool for Gasoline Adulteration

2013 ◽  
Vol 85 (3) ◽  
pp. 1846-1851 ◽  
Author(s):  
Khay M. Tan ◽  
Ishan Barman ◽  
Narahara C. Dingari ◽  
Gajendra P. Singh ◽  
Tet F. Chia ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Online Monitoring ◽  
Monitoring Tool

Raman spectroscopy as a versatile tool for studying of explicit contribution of anharmonicity

2014 ◽  
Vol 23 (04) ◽  
pp. 1450051 ◽  
Author(s):  
M. R. Joya ◽  
J. D. Gonzalez ◽  
J. Barba-Ortega
Keyword(s):  
Raman Spectroscopy ◽  
Thermal Expansion ◽  
Physical Properties ◽  
Raman Spectra ◽  
Lead Titanate ◽  
Ferroelectric Ceramics ◽  
Effect Of Temperature ◽  
Grüneisen Parameter ◽  
Versatile Tool ◽  
Temperature And Pressure

The Raman spectroscopy can be used as a didactic technique in the study of the thermal expansion of Grüneisen parameter and anharmonic behavior of materials. Here, we present exemplary, the lead titanate doped with lanthanum (PLT). However, this method can be used to study various materials. The ferroelectric ceramics have been one of the most studied compounds in the literature both in obtaining the same, as in behavior of chemical and physical properties. The structural PbTiO 3 phase may change either by the application of pressure, temperature or concentration, thereby allowing a study of behavior anharmonic of the material [M. R. Joya, J. Barba and P. S. Pizani, J. Appl. Phys.113 (2013) 013512]. This paper gives a brief review of the effect of temperature and pressure on Raman spectra.

Online Monitoring of Suspension Polymerization Reactions Using Raman Spectroscopy

2004 ◽  
Vol 43 (23) ◽  
pp. 7282-7289 ◽  
Author(s):  
Juliana C. Santos ◽  
Marlon M. Reis ◽  
Ricardo A. F. Machado ◽  
Ariovaldo Bolzan ◽  
Claudia Sayer ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Online Monitoring ◽  
Suspension Polymerization

Application of spectroscopic methods for identification (FT-IR, Raman spectroscopy) and determination (UV, EPR) of quercetin-3-O-rutinoside. Experimental and DFT based approach

2015 ◽  
Vol 140 ◽  
pp. 132-139 ◽  
Author(s):  
Magdalena Paczkowska ◽  
Kornelia Lewandowska ◽  
Waldemar Bednarski ◽  
Mikołaj Mizera ◽  
Agnieszka Podborska ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Spectroscopic Methods ◽  
Ft Ir

scholarly journals Anionic Copolymerization of Styrene Sulfide with Elemental Sulfur (S8)

Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2597
Author(s):  
Jakub Wręczycki ◽  
Dariusz M. Bieliński ◽  
Marcin Kozanecki ◽  
Paulina Maczugowska ◽  
Grzegorz Mlostoń
Keyword(s):  
Thermal Stability ◽  
Raman Spectroscopy ◽  
Ring Opening ◽  
Chemical Structure ◽  
Elemental Sulfur ◽  
Ring Opening Polymerization ◽  
Spectroscopic Methods ◽  
Initial Ratio ◽  
Anionic Copolymerization ◽  
Thermal Stability Of

The superior ability of thiiranes (episulfides) to undergo ring-opening polymerization (ROP) in the presence of anionic initiators allows the preparation of chemically stable polysulfide homopolymers. Incorporation of elemental sulfur (S8) by copolymerization below the floor temperature of S8 permits the placement of a large quantity of sulfur atoms in the polysulfide mainchain. The utility of styrene sulfide (2-phenylthiirane; StS) for copolymerization with elemental sulfur is reported here. A few polysulfides differing depending on the initial ratio of S8 to StS and copolymerization time were synthesized. Various spectroscopic methods (1H NMR, 13C NMR, Raman spectroscopy and FTIR spectroscopy) were applied to characterize the chemical structure of the copolymers. Additionally, the phase structure and thermal stability of the synthesized polysulfides were investigated using DSC and TGA, respectively. The successful anionic copolymerization of styrene sulfide and elemental sulfur has been demonstrated.

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