Larvicidal activity of green synthesized silver nanoparticles and chitosan nanoparticles encapsulated Aloe vera gel extract against Musca domestica (Diptera: Muscidae)

2021 ◽  
Vol 14 ◽  
Author(s):  
Olfat M. El-Monairy ◽  
Ahmed A. El-Sayed ◽  
Manar M. Emara ◽  
Abla D. Abdel-Meguid

Background: Metal nanoparticles synthesized by various chemical reactions have significantly gained worldwide interest for their use in biomedical applications. These processes exhibit a broad range of toxicity in none target organisms. To avoid the chemical toxicity, green synthesis of metal nanoparticles is proposed as a cost-effective and eco-friendly alternative. Aloe vera (A. vera) leaf extract is a medicinal agent with multiple properties including antibacterial effect. Its constituents include lignin, pectin and hemicellulose which can be used in the reduction of silver ions to produce silver nanoparticles (AgNPs). Objective: The aim of this article is to study the use of naturally occurring compounds as a reducing and stabilizing agent for the biosynthesis of nanoparticles and to investigate the insecticidal activity of these compounds against Musca domestica (M. domestica) larvae. Methods: Phytochemical analysis of A. vera gel extract was performed and the phytochemical components identified by Gas chromatography–mass spectrometry (GC-MS) analysis. AgNPs and encapsulated chitosan nanoparticles (CsNPs) were prepared by an eco-friendly method using A. vera gel extract as a reducing and stabilizing agent. A. vera-AgNPs and A. vera encapsulated CsNPs were characterized using Ultraviolet-visible spectrophotometer (UV–vis spectrum), Transmission Electron Microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Then, the insecticidal activity of these compounds was investigated against late second instar larvae of house fly. Results: : The most active ingredients identified by GC-MS analysis were Terpene and Sesquiterpens hydrocarbons. The synthesized AgNPs were spherical with average size about 12-75 nm as revealed by TEM. While encapsulated CsNPs were ranged between 34-75 nm and the shape seems spherical with dark parts confirmed the capsulated plant extract. FTIR and XRD confirmed the successful encapsulation of the gel extract within the chitosan nanoparticles. Results proved the insecticidal potential of the tested compounds against M. domestica larvae and the relative potency of encapsulated CsNPs was nearly 148.51 times more potent than A. vera crude extract whereas, AgNPs was nearly 40.65 times more potent than A. vera crude extract. Furthermore, a prolongation of larval duration and reduction in the percentage pupation and adult emergence were observed. Conclusion: Overall, green-synthesized silver and chitosan nanoparticles are used as a biopesticide for house fly control through safer and cost-effective approach.

2009 ◽  
Vol 106 (1) ◽  
pp. 207-212 ◽  
Author(s):  
Sara M. Palacios ◽  
Alberto Bertoni ◽  
Yanina Rossi ◽  
Rocío Santander ◽  
Alejandro Urzúa

RSC Advances ◽  
2017 ◽  
Vol 7 (1) ◽  
pp. 180-189 ◽  
Author(s):  
Maryam Bordbar

Silver nanoparticles have been immobilized on the surface of waste almond shell using Ruta graveolens sleeves extract as a reducing and stabilizing agent in the present work.


2020 ◽  
Vol 9 (1) ◽  
pp. 451-461 ◽  
Author(s):  
Abid Hussain ◽  
Ansar Mehmood ◽  
Ghulam Murtaza ◽  
Khawaja Shafique Ahmad ◽  
Aneela Ulfat ◽  
...  

AbstractIn this study, we reported an easy, rapid, cost-effective and environmentally benign method for the fabrication of silver nanoparticles (Ag-NPs) using Olea ferruginea as reducing, capping and stabilizing agent. For this, an aqueous extract of leaf and bark of O. ferruginea was treated with 1 mM AgNO3, which reduces Ag ions to Ag-NPs by establishing reddish brown color. The synthesized Ag-NPs were spherical crystals, with a mean size of 23 and 17 nm for leaf- and bark-mediated Ag-NPs, respectively. Fourier transform infrared spectroscopy affirmed the role of leaf and bark extracts of O. ferruginea as reducing, capping and stabilizing agent. These biosynthesized Ag-NPs showed profound antibacterial activity against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Streptococcus pneumonia and Staphylococcus aureus) bacteria. The highest antibacterial activity was shown by bark Ag-NPs against S. aureus (14.00 mm), while leaf Ag-NPs showed higher activity against S. pneumonia (13.00 mm). Additionally, they produced effective antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) as compared to plant extracts and positive control. It was observed that the bark-mediated Ag-NPs had higher percentage (90%) of scavenging potential than the leaf-mediated Ag-NPs (78%). The significance of the current study is the synthesis of eco-friendly, easy and cost-effective Ag-NPs as biomedical products.


2011 ◽  
Vol 111 (6) ◽  
pp. 2439-2448 ◽  
Author(s):  
Chinnaperumal Kamaraj ◽  
Govindasamy Rajakumar ◽  
Abdul Abdul Rahuman ◽  
Kanayairam Velayutham ◽  
Asokan Bagavan ◽  
...  

2019 ◽  
Vol 55 (55) ◽  
pp. 7990-7993 ◽  
Author(s):  
Nathaniel E. Larm ◽  
Dronareddy Madugula ◽  
Mark W. Lee ◽  
Gary A. Baker

Small (<5 nm) gold and silver nanoparticles and their bimetallic counterparts were prepared using the fundamental boron cluster [closo-B10H10]2− as the reducing and stabilizing agent.


Author(s):  
Wilson Mbiti Njue ◽  
Jackson Kilonzo Kithokoi ◽  
Jane Mburu ◽  
Henry Mwangi ◽  
Sauda Swaleh

Metal nanoparticles in the field of nanotechnology are of great interest to modern scientific research due to their size effects, medical uses and, catalytic, electronic and optical properties. Green synthesis of metal nanoparticles is a feasible alternative to chemical methods as it is environmentally friendly and cost effective. In continuation with our research on green synthesis of silver nanoparticles using Kenyan medicinal plants, we here report the synthesis of novel silver nanoparticles (AgNPs) on ultrasonic bath using Adansonia digitata leaves extracts and analysis of their antibacterial activity. The nanoparticles were characterized by UV-Vis, High Resolution Transmission Electron Microscopy (HRTEM), FTIR spectroscopy and Energy Dispersive X-ray (EDX). EDX analysis affirmed the nanoparticles were pure silver. Crystalline nature of the nanoparticles was confirmed by bright circular spots in the Selected Area Electron Diffraction (SAED) in HRTEM image. The AgNPs were spherical with an average size 13 nm. FTIR analysis showed strong –C=C- and –OH stretching bands due to compounds capping the nanoparticles. The synthesized AgNPs showed high inhibition zones of 17.1±0.130 mm towards Gram-negative bacteria E. coli and 12.9±0.082mm towards Gram positive bacteria S. aureus. The aqueous A. digitata extract had no effect on growth inhibition of test bacteria. The study showed that the silver nanoparticles synthesized from the plant’s leaves extract had antibacterial activity against both Gram negative and positive pathogenic bacteria. The nanoparticles can be utilized towards developing novel drugs useful in combating pathogens.


2019 ◽  
Vol 4 (2) ◽  
pp. 112-124
Author(s):  
Edward K.B. Bragais ◽  
Lynne M. Labaclado

Background and Objective: In this study, dudoa (Hydnocarpus alcalae C.DC.) leaf extract was used as a reducing and stabilizing agent in a novel one-step green synthesis of silver nanoparticles. Dudoa is an endemic plant in the province of Legazpi, Philippines and its seed oil was used as an anti-leprotic drug. Method: Therefore, the dudoa leaf extract was used to synthesize silver nanoparticles. Moreover, optimization of various parameters greatly affected the size and morphology of the synthesized AgNPs as indicated by the Ultraviolet-visible (UV-vis) spectrophotometry. The synthesized AgNPs were further characterized using spectral analyses such as XRD, EDX, SEM, FT-IR, TGA and DLS. The antimicrobial activity of synthesized AgNPs was also demonstrated. Results and Discussions: The synthesized AgNPs exhibited a diffraction pattern and a particle size ranging from 22-48 nm. The AgNPs also showed complete inhibitory and mild reactivity against representative pathogenic gram-positive (S.aureus) and gram-negative (E.coli) bacteria. Conclusion: Silver nanoparticles were successfully synthesized using H. alcalae leaf extract. Furthermore, this green synthesis approach appeared to be cost-effective, non-toxic, and eco-friendly which is a best alternative to the conventional chemical methods.


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