Leptospira patógenas en murciélagos de Campeche y Yucatán, México

Revista Mvz Córdoba ◽

10.21897/rmvz.1815 ◽

2020 ◽

pp. e1815

Author(s):

Marco Torres-Castro ◽

Viviana Febles-Solís ◽

Silvia Hernández-Betancourt ◽

Henry Noh-Pech ◽

Erendira Estrella ◽

...

Keyword(s):

Artibeus Jamaicensis ◽

Pteronotus Parnellii ◽

16S Rna ◽

Leptospira Borgpetersenii

Objetivo. Reportar la infección con Leptospira en riñones de murciélagos de Campeche y Yucatán, México, a través de la amplificación por PCR de dos fragmentos distintos del gen 16S RNA ribosomal. Materiales y métodos. Se realizaron capturas en un sitio de Campeche y dos de Yucatán. A los murciélagos capturados se les aplicó la eutanasia y se les realizó una necropsia para recolectar tejido renal que se usó en la extracción de ADN total. Se realizaron dos PCR convencionales para la amplificación de los fragmentos de 16S RNA ribosomal. Se obtuvieron las secuencias de algunos productos positivos y se analizaron con herramientas bioinformáticas para identificar la especie infectante de Leptospira. Resultados. Se capturaron 69 murciélagos pertenecientes a cuatro familias y a ocho especies distintas. La familia con mayor diversidad fue Phyllostomidae con cinco especies. La especie con mayor frecuencia de captura fue Artibeus jamaicensis (59.4%). Las PCR arrojaron una frecuencia de infección global de 21.7%. Las especies infectadas fueron A. jamaicensis, Pteronotus parnellii y Chiroderma villosum. El análisis bioinformático arrojó un 99.0% de identidad para Leptospira noguchii, Leptospira borgpetersenii y Leptospira santarosai. Conclusiones. Algunas especies de murciélagos de Yucatán y Campeche son portadores renales de leptospiras patógenas, por lo que pueden participar en el ciclo silvestre de transmisión en la región. La frecuencia de infección encontrada en los riñones de los murciélagos utilizados es mayor en comparación con aquellas obtenidas en otros reservorios de Yucatán y Campeche. Nuevas especies de murciélagos son reportadas como portadores de Leptospira para México.

Download Full-text

Análisis de la colección de murciélagos (Mammalia: Chiroptera) del Museo Nacional de Historia Natural de Santo Domingo

Novitates Caribaea ◽

10.33800/nc.v0i4.160 ◽

2011 ◽

pp. 109-119 ◽

Cited By ~ 3

Author(s):

Miguel S. Núñez-Novas ◽

Yolanda M. León

Keyword(s):

Santo Domingo ◽

Artibeus Jamaicensis ◽

Pteronotus Parnellii ◽

Historia Natural ◽

Distribución Geográfica ◽

Molossus Molossus

Se hace la descripción de la colección de murciélagos del Museo Nacional de Historia Natural de Santo Domingo. Se atiende a la composición de la colección y la distribución geográfica de sus especímenes; se hace una actualización taxonómica. Se examinaron 723 especímenes, pertenecientes a seis (6) familias, 17 géneros y 18 especies, estando representadas todas las especies reportadas para La Hispaniola. Todos los géneros son monoespecíficos, con excepción de Pteronotus Gray, 1838 con dos especies. Las fechas de colecta datan desde 1961 y se extienden hasta el 2008, correspondiendo la mayor parte de registros a la Región Suroeste de la República Dominicana. Las especies más abundantes en la colección resultaron ser generalistas en cuanto a selección de refugios, tales como los filostómidos Artibeus jamaicensis y Macrotus waterhousii y el molósido Molossus molossus. Cabe destacar la presencia de especies de cuevas calientes (Pteronotus parnellii, P. quadridens, y Phyllonycteris poeyi), así como especies raras: Chilonatalus micropus, Lasiurus minor y Natalus major.

Download Full-text

Horas de éxodo y estacionalidad de los murciélagos en cuatro cuevas de República Dominicana

Novitates Caribaea ◽

10.33800/nc.v0i7.68 ◽

2014 ◽

pp. 83-94

Author(s):

Miguel S. Núñez-Novas ◽

Yolanda M. León ◽

Jeannette Mateo ◽

Liliana M. Dávalos

Keyword(s):

Puerto Rico ◽

Eptesicus Fuscus ◽

Artibeus Jamaicensis ◽

Pteronotus Parnellii

Se estudiaron los patrones de emergencia y estados reproductivos de las poblaciones de murciélagos en cuatro cuevas de la República Dominicana. Se capturó un total de 12 especies: Mormoops blainvillei, Pteronotus parnellii, Pteronotus quadridens, Chilonatalus micropus, Natalus major, Artibeus jamaicensis, Brachyphylla pumila, Erophylla bombifrons, Macrotus waterhousii, Monophyllus redmani, Phyllonycteris poeyi y Eptesicus fuscus. Los murciélagos se capturaron de forma más o menos continua entre las 18:15 y 23:00h. De un total de 1,445 ejemplares a los cuales se les determinó el sexo, 969 fueron machos (67.1%). Los resultados obtenidos con respecto a la actividad reproductiva muestran cómo las especies presentes en las cuevas se encuentran en estados reproductivos diferentes, registrándose ejemplares preñadas o lactantes, pertenecientes a dos familias dominantes: Mormopidae y Phyllostomidae. Además, que se contrastaron reportes de Cuba y Puerto Rico donde se muestra que algunas especies podrían tener épocas reproductivas más largas que las reportadas actualmente.

Download Full-text

Dengue virus in Mexican bats

Epidemiology and Infection ◽

10.1017/s0950268808000460 ◽

2008 ◽

Vol 136 (12) ◽

pp. 1678-1683 ◽

Cited By ~ 29

Author(s):

Á. AGUILAR-SETIÉN ◽

M. L. ROMERO-ALMARAZ ◽

C. SÁNCHEZ-HERNÁNDEZ ◽

R. FIGUEROA ◽

L. P. JUÁREZ-PALMA ◽

...

Keyword(s):

Dengue Virus ◽

Rainy Season ◽

Gulf Coast ◽

Ns1 Protein ◽

Rt Pcr ◽

Artibeus Jamaicensis ◽

Pteronotus Parnellii ◽

Endemic Areas

SUMMARYIndividuals belonging to five families, 12 genera, and 19 different species of bats from dengue endemic areas in the Gulf and Pacific coasts of Mexico were examined by ELISA, RT–PCR, and for the presence of dengue virus (DV) NS1 protein. Nine individuals from four species were seropositive by ELISA: three insectivorous,Myotis nigricans(four positives/12 examined),Pteronotus parnellii(3/19), andNatalus stramineus(1/4), and one frugivorousArtibeus jamaicensis(1/35) (12·86% seroprevalence in positive species). DV serotype 2 was detected by RT–PCR in four samples from three species (all from the Gulf coast – rainy season): two frugivorous,A. jamaicensis(2/9), andCarollia brevicauda(1/2), and one insectivorous,M. nigricans(1/11). The latter was simultaneously positive for NS1 protein. DV RT–PCR positive animals were all antibody seronegative.M. nigricansshowed positive individuals for all three tests. This is the first evidence suggesting the presence of DV in bats from Mexico.

Download Full-text

Structural Role of rRNAs on the Ribosomal Subunits from E. Coli by Scanning Transmission Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100098629 ◽

1981 ◽

Vol 39 ◽

pp. 424-425

Author(s):

M. Boublik ◽

N. Robakis ◽

J.S. Wall

Keyword(s):

Three Dimensional ◽

Uranyl Acetate ◽

Dimensional Structure ◽

Electron Microscopic ◽

Ribosomal Subunits ◽

E Coli ◽

Freeze Dried ◽

16S Rna ◽

Scanning Transmission ◽

And Function

The three-dimensional structure and function of biological supramolecular complexes are, in general, determined and stabilized by conformation and interactions of their macromolecular components. In the case of ribosomes, it has been suggested that one of the functions of ribosomal RNAs is to act as a scaffold maintaining the shape of the ribosomal subunits. In order to investigate this question, we have conducted a comparative TEM and STEM study of the structure of the small 30S subunit of E. coli and its 16S RNA.The conventional electron microscopic imaging of nucleic acids is performed by spreading them in the presence of protein or detergent; the particles are contrasted by electron dense solution (uranyl acetate) or by shadowing with metal (tungsten). By using the STEM on freeze-dried specimens we have avoided the shearing forces of the spreading, and minimized both the collapse of rRNA due to air drying and the loss of resolution due to staining or shadowing. Figure 1, is a conventional (TEM) electron micrograph of 30S E. coli subunits contrasted with uranyl acetate.

Download Full-text

Protein-induced conformational changes in 16S rRNA during assembly of the Escherichia Coli 30S ribosomal subunit: A STEM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128833 ◽

1987 ◽

Vol 45 ◽

pp. 910-911

Author(s):

M. Boublik ◽

V. Mandiyan ◽

J.F. Hainfeld ◽

J.S. Wall

Keyword(s):

16S Rrna ◽

Conformational Changes ◽

Ribosomal Proteins ◽

Structural Changes ◽

Ribosomal Subunit ◽

Compact Structure ◽

E Coli ◽

Freeze Dried ◽

16S Rna ◽

30S Subunit

The aim of this study is to understand the mechanism of 16S rRNA folding into the compact structure of the small 30S subunit of E. coli ribosome. The assembly of the 30S E. coli ribosomal subunit is a sequence of specific interactions of 16S rRNA with 21 ribosomal proteins (S1-S21). Using dedicated high resolution STEM we have monitored structural changes induced in 16S rRNA by the proteins S4, S8, S15 and S20 which are involved in the initial steps of 30S subunit assembly. S4 is the first protein to bind directly and stoichiometrically to 16S rRNA. Direct binding also occurs individually between 16S RNA and S8 and S15. However, binding of S20 requires the presence of S4 and S8. The RNA-protein complexes are prepared by the standard reconstitution procedure, dialyzed against 60 mM KCl, 2 mM Mg(OAc)2, 10 mM-Hepes-KOH pH 7.5 (Buffer A), freeze-dried and observed unstained in dark field at -160°.

Download Full-text

Imaging of ribosomal RNA-protein interactions by dark-field STEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100153221 ◽

1989 ◽

Vol 47 ◽

pp. 250-251

Author(s):

M. Boublik ◽

V. Mandiyan ◽

S. Tumminia ◽

J.F. Hainfeld ◽

J.S. Wall

Keyword(s):

Nucleic Acid ◽

16S Rrna ◽

Dark Field ◽

Radius Of Gyration ◽

Central Domain ◽

The Core ◽

16S Rna ◽

30S Subunit ◽

Core Particles

Success in protein-free deposition of native nucleic acid molecules from solutions of selected ionic conditions prompted attempts for high resolution imaging of nucleic acid interactions with proteins, not attainable by conventional EM. Since the nucleic acid molecules can be visualized in the dark-field STEM mode without contrasting by heavy atoms, the established linearity between scattering cross-section and molecular weight can be applied to the determination of their molecular mass (M) linear density (M/L), mass distribution and radius of gyration (RG). Determination of these parameters promotes electron microscopic imaging of biological macromolecules by STEM to a quantitative analytical level. This technique is applied to study the mechanism of 16S rRNA folding during the assembly process of the 30S ribosomal subunit of E. coli. The sequential addition of protein S4 which binds to the 5'end of the 16S rRNA and S8 and S15 which bind to the central domain of the molecule leads to a corresponding increase of mass and increased coiling of the 16S rRNA in the core particles. This increased compactness is evident from the decrease in RG values from 114Å to 91Å (in “ribosomal” buffer consisting of 10 mM Hepes pH 7.6, 60 mM KCl, 2 m Mg(OAc)2, 1 mM DTT). The binding of S20, S17 and S7 which interact with the 5'domain, the central domain and the 3'domain, respectively, continues the trend of mass increase. However, the RG values of the core particles exhibit a reverse trend, an increase to 108Å. In addition, the binding of S7 leads to the formation of a globular mass cluster with a diameter of about 115Å and a mass of ∽300 kDa. The rest of the mass, about 330 kDa, remains loosely coiled giving the particle a “medusa-like” appearance. These results provide direct evidence that 16S RNA undergoes significant structural reorganization during the 30S subunit assembly and show that its interactions with the six primary binding proteins are not sufficient for 16S rRNA coiling into particles resembling the native 30S subunit, contrary to what has been reported in the literature.

Download Full-text