Antibacterial Effect of Azadirachta indica (Neem) or Curcuma longa (Turmeric) against Enterococcus faecalis Compared with That of 5% Sodium Hypochlorite or 2% Chlorhexidine in vitro

Objective. To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results. The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions. In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.

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Antibacterial Efficacy of Neem (Azadirachta indica) Extract against Enterococcus faecalis: An in vitro Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1932 ◽

2016 ◽

Vol 17 (10) ◽

pp. 791-794 ◽

Cited By ~ 3

Author(s):

Mohammed Mustafa

Keyword(s):

Enterococcus Faecalis ◽

Analysis Of Variance ◽

Sodium Hypochlorite ◽

Azadirachta Indica ◽

Leaf Extract ◽

Antimicrobial Efficacy ◽

Antibacterial Efficacy ◽

Zones Of Inhibition ◽

Neem Leaf Extract

ABSTRACT Introduction Debridement and disinfection of the root canal is a crucial step in the success of endodontic treatment. Several antimicrobial agents alone or in combination are used to achieve this. The objective of this in vitro study was to assess the antimicrobial efficacy of neem (Azadirachta indica) extract against Enterococcus faecalis. Materials and methods Neem leaf extract, 2% chlorhexidine, 3% sodium hypochlorite were used to assess the antimicrobial efficiency. Agar well diffusion test was used to study the antimicrobial efficacy with saline as control. The zone of inhibition was recorded, tabulated, and analyzed statistically with the help of IBM Statistical Package for the Social Sciences statistics version 20 using analysis of variance test. Results All the three medicaments showed well-defined and comparable zones of inhibition around their respective wells. All values were significantly higher than the control group. Analysis of variance showed significant difference between zone diameters of chlorhexidine, neem leaf extract, and 3% sodium hypochlorite against E. faecalis (p < 0.05). Conclusion From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and sodium hypochlorite. Clinical significance Neem leaf extract has significant antimicrobial activity against E. faecalis and thus opens the perspectives for the use of neem extract as an intracanal medication. How to cite this article Mustafa M. Antibacterial Efficacy of Neem (Azadirachta indica) Extract against Enterococcus faecalis: An in vitro Study. J Contemp Dent Pract 2016;17(10):791-794.

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Antibacterial effect of ozonated olive oil versus sodium hypochlorite against Enterococcus faecalis bacteria: In vitro study

Egyptian Dental Journal ◽

10.21608/edj.2019.74003 ◽

2019 ◽

Vol 65 (4) ◽

pp. 3271-3276

Author(s):

Nada Wassef ◽

Marwa Fouad

Keyword(s):

Olive Oil ◽

Enterococcus Faecalis ◽

Sodium Hypochlorite ◽

Antibacterial Effect ◽

In Vitro Study ◽

Vitro Study

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Antibacterial efficacy of Melaleuca alternifolia (Tea tree oil), Curcuma longa (Turmeric), 2% chlorhexidine, and 5% sodium hypochlorite against Enterococcus faecalis: An in vitro study

Saudi Endodontic Journal ◽

10.4103/1658-5984.163623 ◽

2015 ◽

Vol 5 (3) ◽

pp. 182 ◽

Cited By ~ 3

Author(s):

Agrima Vasudeva ◽

Natasha Jaiswal ◽

Paridhi Garg ◽

ShashiPrabha Tyagi ◽

Jagvir Singh ◽

...

Keyword(s):

Enterococcus Faecalis ◽

Sodium Hypochlorite ◽

Curcuma Longa ◽

In Vitro Study ◽

Vitro Study ◽

Antibacterial Efficacy ◽

Tea Tree Oil ◽

Melaleuca Alternifolia ◽

Tea Tree

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Antibacterial effect of sodium hypochlorite gel on Enterococcus faecalis in endodontics: A systematic review

International Dental Research ◽

10.5577/intdentres.2020.vol10.no1.5 ◽

2020 ◽

Vol 10 (1) ◽

pp. 22-29

Author(s):

Salma Fuad Al Nesser ◽

Nada George Bshara ◽

Özkan Adıgüzel

Keyword(s):

Systematic Review ◽

Enterococcus Faecalis ◽

Sodium Hypochlorite ◽

Root Canal ◽

Antibacterial Effect ◽

Risk Of Bias ◽

Publication Date ◽

Sodium Hypochlorite Solution ◽

Root Canal Irrigant

Aim: The aim of this systematic review was to investigate the in vitro antibacterial effect of sodium hypochlorite gel on Enterococcus faecalis during root canal treatment. Methods: An electronic databases (PubMed, ScienceDirect, Google Scholar, CENTRAL, Health Advance) was performed (last updated was 1st April 2019). The reference lists of the included studies were hand-searched. Randomized in vitro studies that compared sodium hypochlorite gel to solution as a root canal irrigant were included. No limitation on publication date. Results: Out of 20741 articles, only two studies with 116 human teeth were included in this review. The studies showed 50% high risk of bias, and 50% medium risk of bias for the included studies. Both sodium hypochlorite gel and solution had some bacterial growth inhibition over E. faecalis. The heterogeneity in methodology of the included studies and the lack of high evidence led to contradictory results. However, sodium hypochlorite solution was better in enhancing the antibacterial effect according to the robust study. Conclusion: There is insufficient reliable evidence about the antibacterial effect of sodium hypochlorite gel on enterococcus faecalis. Although the robust study included in this review revealed that sodium hypochlorite gel is less efficient than the solution as a root canal irrigant, further studies are needed to consider the most effective type, concentration, duration and treatment protocols in enhancing the antibacterial effect of sodium hypochlorite. How to cite this article: Al Nesser S, Bshara N, Adıgüzel Ö. Antibacterial effect of sodium hypochlorite gel on Enterococcus faecalis in endodontics: A systematic review. Int Dent Res 2020;10(1):22-29. https://doi.org/10.5577/intdentres.2020.vol10.no1.3 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

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