scholarly journals Analysis of Whole Cell Protein Profiles by SDS-PAGE to Identify Indigenous Cellulose-producer Acetic Acid Bacteria

2017 ◽  
Vol 21 (2) ◽  
pp. 86
Author(s):  
Sarkono Sarkono ◽  
Soekarti Moeljopawiro ◽  
Bambang Setiaji ◽  
Langkah Sembiring
Keyword(s):  
Acetic Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Acetic Acid Bacteria ◽  
Cellular Protein ◽  
Cell Protein ◽  
Phenotypic Traits ◽  
Protein Profiles ◽  
Bacterial Isolates ◽  
Sds Page

This study was carried out to analyze the suitability of the identification of four indigenous cellulose-producing acetic acid bacterial isolates (ANG29, KRE65, ANG32 and SAL53) based on the analysis of whole cellular protein profiles against identification based on phenotypic traits. Whole cellular protein profiles were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. The whole cellular protein profiles obtained from sample isolates, were compared with reference isolates for species identification. The results showed that based on visual observations can be determined as much as 12 bands of protein with a molecular weight of 19,099 KDa up to 132.182 KDa. Based on the analysis of protein bands were detected visually, fourth indigenous cellulose- producing acetic acid bacterial isolates in the study had a higher similarity profile to the reference strain Gluconacetobacter xylinus BTCC 769 compared with other reference strains namely G. hansenii NBRC 14820T. This condition is consistent with the results of the identification of fourth cellulose producing acetic acid bacterial isolates based on phenotypic traits. Thus, the whole cellular protein profiles by SDS-PAGE technique can be used as a one of method to identification of cellulose producing acetic acid bacterial isolates.

scholarly journals  Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 259-263 ◽  
Author(s):  
A. Aksakal
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Visual Assessment ◽  
Cell Protein ◽  
Protein Profiles ◽  
Whole Cell ◽  
Sds Page ◽  
Molecular Masses ◽  
Whole Cell Protein ◽  
Salmonella Serovars

This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1, 33.9, 30.7, 27.6, 25.4, and 24 kDa were detected in all Salmonella serovars. Salmonella strains used in this study were closely related and could not be differentiated depending on the whole cell protein profiles using SDS-PAGE.

Whole-cell protein and ITS rDNA profiles as diagnostic tools to discriminate Fusarium avenaceum intraspecific variability and associated virulence

2009 ◽  
Vol 55 (2) ◽  
pp. 117-125 ◽  
Author(s):  
V. Vujanovic ◽  
S. Vidovic ◽  
M. R. Fernandez ◽  
P. Daida ◽  
D. Korber
Keyword(s):  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Intraspecific Variability ◽  
Fusarium Avenaceum ◽  
Cell Protein ◽  
Diagnostic Tools ◽  
Group Method ◽  
Head Blight ◽  
Sds Page

A total of 91 isolates of Fusarium avenaceum were regrouped into 15 phenotypes and 10 vegetative compatibility groups showing specific one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1-D SDS–PAGE) protein profiles and less-specific internal transcribed spacer rDNA profiles. Each isolate possessed reproducible signature protein bands. Indeed, the unweighted pair group method with arithmetic averages clustering revealed that the protein profile of each group of isolates correlated with fungus virulence. The use of SDS–PAGE offers a simple and sensitive technique for routine differentiation between pathogenic and nonpathogenic isolates within unknown F. avenaceum populations. The discovery has significant implications for risk assessment of cereal yield to ensure food and feed safety. This low-cost approach has the potential to be optimized and extended to a broad spectrum of Fusarium head blight pathogens.

scholarly journals Use of Seed Protein Profiles to Characterize Peanut Cultivars1

1994 ◽  
Vol 21 (2) ◽  
pp. 152-159 ◽  
Author(s):  
C. M. Bianchi-Hall ◽  
R. D. Keys ◽  
H. T. Stalker
Keyword(s):  
Seed Protein ◽  
Cultivar Identification ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Genetic Material ◽  
Seed Storage Proteins ◽  
Molecular Techniques ◽  
Protein Profiles ◽  
Sds Page

Abstract In the last 10 to 15 yr, the development of biotechnology and molecular techniques has allowed great advancements toward the identification of cultivars among plant species. In legumes, the success of cultivar identification depends on the species under investigation, the type and variability of genetic material found in cultivars, and the technology used for investigations. In this study, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to assess diversity of peanut (Arachis hypogaea L.) seed protein profiles. The objectives of this investigation were a) to assess diversity of protein profiles in peanuts for cultivar identification using SDS-PAGE and b) to determine the extent of variability of seed storage proteins (SSP) among samples of cultivars originating from different locations. The first study included 34 cultivars grown at Lewiston, NC and the second one included nine cultivars grown at six locations. The results of both studies indicated that it is possible to differentiate between subspecies but not to associate a particular profile with only one specific cultivar. Within subspecies, cultivars clustered in more than one group and most cultivars that grouped together were genetically related.

A simple and rapid method for the differentiation and identification of thermophilic bacteria

2006 ◽  
Vol 52 (8) ◽  
pp. 753-758 ◽  
Author(s):  
Bin Liu ◽  
Hebin Li ◽  
Suijie Wu ◽  
Xiaobo Zhang ◽  
Lianhui Xie
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Random Amplified Polymorphic Dna ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cell Protein ◽  
Protein Patterns ◽  
Simple Method ◽  
Whole Cell ◽  
Sds Page

In total, 170 strains of thermophilic bacteria were isolated from deep-sea hydrothermal fields in the Pacific Ocean and a hot spring in Xiamen of China. To facilitate the identification of thermophilic strains, sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS–PAGE) of whole-cell proteins of these strains was first performed. The results showed that there exist four different protein patterns, indicating that the 170 strains might belong to four species or genera. The RAPD (random amplified polymorphic DNA) profiles of nine representative strains were consistent with those of SDS–PAGE. To further identify the species of the nine strains, their 16S rDNA sequences were analyzed. The results showed that the nine strains fell into four species of three genera, which was the same as revealed by SDS–PAGE. Therefore, SDS–PAGE of whole-cell proteins could be used as a rapid and simple method for the discrimination of thermophilic bacteria as the first step of species identification.Key words: thermophilic bacteria, SDS–PAGE, whole-cell protein, discrimination.

Phenotypic and genetic diversity of enterococci isolated from Italian cheeses

2001 ◽  
Vol 68 (2) ◽  
pp. 303-316 ◽  
Author(s):  
CHRISTIAN ANDRIGHETTO ◽  
EDO KNIJFF ◽  
ANGIOLELLA LOMBARDI ◽  
SANDRA TORRIANI ◽  
MARC VANCANNEYT ◽  
...  
Keyword(s):  
Species Recognition ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Reference Method ◽  
Antagonistic Activity ◽  
Cell Protein ◽  
Rapd Pcr ◽  
Sds Page ◽  
Carbohydrate Fermentation ◽  
Proteolytic Activities

In the present study, 124 enterococcal strains, isolated from traditional Italian cow, goat and buffalo cheeses, were characterized using phenotypic features and randomly amplified polymorphic DNA polymerase chain reaction (RAPD–PCR). The RAPD–PCR profiles obtained with four primers and five different amplification conditions were compared by numerical analysis and allowed an inter- and intraspecific differentiation of the isolates. Whole-cell protein analysis by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) was used as a reference method for species identification. The strains were identified as Enterococcus faecalis (82 strains), E. faecium (27 strains), E. durans (nine strains), E. gallinarum (four strains) and E. hirae (two strains). Species recognition by means of RAPD–PCR was in agreement with the SDS–PAGE results except for eight strains of E. faecium that clustered in separated groups. On the other hand, phenotypic identification based on carbohydrate fermentation profiles, using the rapid ID 32 STREP galleries, gave different results from SDS–PAGE in 12·1% of the cases. The majority of the strains had weak acidifying and proteolytic activities in milk. One E. faecium strain showed vanA (vancomycin resistance) genotype while four strains showed a β-haemolytic reaction on human blood. Several strains showed antagonistic activity towards indicator strains of Listeria innocua, Clostridium tyrobutyricum and Propionibacterium freudenreichii subsp. shermanii.

scholarly journals Assessment of Some Bacteria from Panteka Stream, Kaduna, Nigeria, for their Larvicidal Activity Against Anopheles gambiae

2019 ◽  
Vol 3 (12) ◽  
pp. 149-154
Author(s):  
Thankgod Ositadinma Ndibe ◽  
Nancy Erika Nwabufo ◽  
Johnson John Usman ◽  
Winnie Chuno Eugene
Keyword(s):  
Anopheles Gambiae ◽  
Larvicidal Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Profiling ◽  
Mosquito Larvae ◽  
Bacterial Isolates ◽  
Sample Collection ◽  
Sds Page ◽  
Biochemical Identification

It is obvious that malaria is one of the commonest diseases in Africa, hence the need to embark on a study to reduce its transmission by eliminating the vector. Some microorganisms are known to have larvicidal activity leading to destruction of mosquito larvae, thereby, preventing them from metamorphosing into adult mosquitoes that can transmit Plasmodium spp. Panteka stream, Kaduna, Nigeria, is a dumping site for refuse and automobile waste and thus, a potential source of bacteria. This present investigation was aimed at screening bacterial isolates for their larvicidal activity against Anopheles gambiae. Standard methods were employed in sample collection, isolation, morphological, biochemical identification and protein profiling of these bacteria isolates. Five different types of bacteria were identified; Bacillus thuringiensis, Staphylococcus aureus, Micrococcus sedentarius, Enterococcus faecalis and Streptococcus pneumonia. Among these bacteria, B. thuringiensis exhibited the most larvicidal activity, followed by M. sedentarius. On the basis of lethal concentration (LC50), B. thuringiensis exhibited the highest lethal activity against Anopheles gambiae larvae at 48 hour duration of exposure. Results showed that concentration of bacterial isolates and duration of exposure of larvae to the bacterial isolates, determine the mortality rate of larvae. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) revealed variable bands between B. thuringiensis and M. sedentarius, which might have accounted for their differences in larvicidal activity. The use of bacteria for the control of mosquito larvae is highly recommended. Further research should be conducted to search for more bacteria and possibly fungi which have potentials for larvicidal activity.

scholarly journals ANALYSIS OF FRUIT BUD PROTEINS ASSOCIATED WITH PLANT DORMANCY

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1068d-1068 ◽  
Author(s):  
Gregory A. Lang ◽  
Joshua Tao
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Cold Treatment ◽  
Sodium Dodecyl ◽  
Growth Chamber ◽  
Physiological Status ◽  
Chilling Requirement ◽  
Protein Profiles ◽  
Sds Page ◽  
Relationship Of ◽  
The Relationship

Plant dormancy research has long been stifled by the lack of appropriate biochemical markers to characterize the changing physiological status of dormant vegetative or reproductive buds. Two sets of experiments were conducted in an attempt to identify changes in soluble protein profiles during endodormancy of peach and blueberry reproductive apices. Bud samples from the peach cultivars `La Festival' (low chilling requirement) and `La White' (moderate chilling requirement) were taken every 15 days in the orchard during December and January, extracted for soluble proteins, and analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Outshoots were forced at 25C in a growth chamber to determine the intensity of endodormancy. A further experiment utilized potted `Bluechip' and `Meader' (troth high chilling requirement) blueberry plants given varying periods of cold (4.5C) chamber treatment, followed by forcing at 25C in a growth chamber. Bud samples were taken following cold treatment for extraction and SDS-PAGE. The relationship of the resulting protein profiles to chilling unit accumulation and intensity of endodormancy will be discussed.

scholarly journals Perbandingan Metode Hierarchical Cluster Analysis untuk Analisis Keragaman Hayati Trypanosoma evansi dari Indonesia Berdasarkan Profil Protein (COMPARISON OF HIERARCHICAL CLUSTER ANALYSIS METHODS FOR BIODIVERSITY ANALYSIS OF TRYPANOSOMA EVANSI

2018 ◽  
Vol 18 (4) ◽  
pp. 516
Author(s):  
Didik Tulus Subekti ◽  
Ichwan Yuniarto ◽  
Sulinawati Sulinawati
Keyword(s):  
Cluster Analysis ◽  
Hierarchical Cluster Analysis ◽  
Binary Data ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Hierarchical Cluster ◽  
Trypanosoma Evansi ◽  
Protein Profiles ◽  
Sds Page

Hierarchical Clustering Analysis (HCA) has long been known to be useful for the analysis of biodiversity of microorganisms based on SDSPAGE protein profile (sodium dodecyl sulfate polyacrylamide gel electrophoresis). However, varying methods of HCA consequently produce variability of analysis results and interpretations. Therefore, it is necessary to evaluate and further determine the most appropriate method which could described the biodiversity based on protein profiles of T.evansi isolates from Indonesia. Eleven isolates of T.evansi from different geographic locations were run on SDS PAGE. Furthermore, SDS PAGE protein profiles from eleven isolates were converted into binary data and analyzed using five different methods of HCA i.e. Average Linkage, Complete Linkage, Single Linkage, Ward Linkage and McQuitty Linkage, respectively.Data were also analyzed by multidimensional scaling (MDS) and densitogram. The analysis showed that the dendrogram constructed with Ward Linkage gives the best results and corresponding with densitogram, MDS and able to describe the geographical origins of isolates.

Heterozygous Abnormal Fibrinogen Osaka III with the Replacement of γ Arginine-275 by Histidine Has an Apparently Higher Molecular Weight γ-Chain Variant

1992 ◽  
Vol 68 (05) ◽  
pp. 534-538 ◽  
Author(s):  
Nobuhiko Yoshida ◽  
Shingi Imaoka ◽  
Hajime Hirata ◽  
Michio Matsuda ◽  
Shinji Asakura
Keyword(s):  
Molecular Weight ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Tetraacetic Acid ◽  
Fibrin Monomer ◽  
Sds Page ◽  
Thrombotic Tendency ◽  
Chain Variant

SummaryCongenitally abnormal fibrinogen Osaka III with the replacement of γ Arg-275 by His was found in a 38-year-old female with no bleeding or thrombotic tendency. Release of fibrinopeptide(s) by thrombin or reptilase was normal, but her thrombin or reptilase time in the absence of calcium was markedly prolonged and the polymerization of preformed fibrin monomer which was prepared by the treatment of fibrinogen with thrombin or reptilase was also markedly defective. Propositus' fibrinogen had normal crosslinking abilities of α- and γ-chains. Analysis of fibrinogen chains on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the system of Laemmli only revealed the presence of abnormal γ-chain with an apparently higher molecular weight, the presence of which was more clearly detected with SDS-PAGE of fibrin monomer obtained by thrombin treatment. Purified fragment D1 of fibrinogen Osaka III also seemed to contain an apparently higher molecular weight fragment D1 γ remnant on Laemmli gels, which was digested faster than the normal control by plasmin in the presence of [ethy-lenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA).

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