scholarly journals ROLE OF REDUCING CELL LEAKAGE IN CELL CULTURE USING LARGE PORE SIZE PERMEABLE MEMBRANE

2017 ◽  
Vol 6 (1) ◽  
pp. 16
Author(s):  
Nur Kaliwantoro ◽  
Marsetyawan HNE Soesatyo ◽  
Indarto Indarto ◽  
Mohammad Juffrie ◽  
Rini Dharmastiti ◽  
...  
Keyword(s):  
Cell Culture ◽  
Vero Cell ◽  
Pore Size ◽  
Flow Chamber ◽  
Permeable Membrane ◽  
Large Pore ◽  
Small Pore ◽  
Large Pore Size ◽  
Cell Leakage

Permeable membranes are widely used in many in vitro studies using cell culture. Some cell leakage are often occurs when using permeable membrane with large pore size. Unfortunately the use of permeable membrane with smaller pore size in permeability studies faces some difficulties due to its small pore size and pore density. Recent study provides the protocol in using permeable membrane with large pore size with smaller cell leakage. Vero cell line (CCL-81, ATCC) was used and culture on polyester permeable membrane with 3 µm pore size. Visualization using inverted microscope was used to analized the cell leakage on the permeable membrane. Parallel plate flow chamber was used to analized the permeability performance of the Vero cell cultured on the permeable membrane. The result showed that the current technique is significant in reducing cell leakage of the cell cultured on large pore size of permeable membrane. The same results were found in using polyester and polycarbonate permeable membrane.

scholarly journals In vitro and in vivo evaluations of the fully porous Ti6Al4V acetabular cups fabricated by a sintering technique

RSC Advances ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 6724-6732 ◽  
Author(s):  
Ji Li ◽  
Wei Li ◽  
Zhongli Li ◽  
Yuxing Wang ◽  
Ruiling Li ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Total Hip Arthroplasty ◽  
Pore Size ◽  
Hip Arthroplasty ◽  
High Porosity ◽  
Total Hip ◽  
Large Pore ◽  
Large Pore Size

The fully porous Ti6Al4V cup fabricated by the sintered technique showed high porosity, large pore size with good mechanical properties. It may be effective in achieving in vivo stability after the total hip arthroplasty.

scholarly journals Osteochondral Tissue Regeneration Using a Tyramine-Modified Bilayered PLGA Scaffold Combined with Articular Chondrocytes in a Porcine Model

2019 ◽  
Vol 20 (2) ◽  
pp. 326 ◽  
Author(s):  
Tzu-Hsiang Lin ◽  
Hsueh-Chun Wang ◽  
Wen-Hui Cheng ◽  
Horng-Chaung Hsu ◽  
Ming-Long Yeh
Keyword(s):  
Articular Cartilage ◽  
Pore Size ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Plga Scaffold ◽  
Small Pore Size ◽  
Large Pore ◽  
Small Pore ◽  
Osteochondral Tissue ◽  
Large Pore Size

Repairing damaged articular cartilage is challenging due to the limited regenerative capacity of hyaline cartilage. In this study, we fabricated a bilayered poly (lactic-co-glycolic acid) (PLGA) scaffold with small (200–300 μm) and large (200–500 μm) pores by salt leaching to stimulate chondrocyte differentiation, cartilage formation, and endochondral ossification. The scaffold surface was treated with tyramine to promote scaffold integration into native tissue. Porcine chondrocytes retained a round shape during differentiation when grown on the small pore size scaffold, and had a fibroblast-like morphology during transdifferentiation in the large pore size scaffold after five days of culture. Tyramine-treated scaffolds with mixed pore sizes seeded with chondrocytes were pressed into three-mm porcine osteochondral defects; tyramine treatment enhanced the adhesion of the small pore size scaffold to osteochondral tissue and increased glycosaminoglycan and collagen type II (Col II) contents, while reducing collagen type X (Col X) production in the cartilage layer. Col X content was higher for scaffolds with a large pore size, which was accompanied by the enhanced generation of subchondral bone. Thus, chondrocytes seeded in tyramine-treated bilayered scaffolds with small and large pores in the upper and lower parts, respectively, can promote osteochondral regeneration and integration for articular cartilage repair.

Cu2O mesoporous spheres with a high internal diffusion capacity and improved catalytic ability for the aza-Henry reaction driven by visible light

2014 ◽  
Vol 50 (91) ◽  
pp. 14237-14240 ◽  
Author(s):  
Jun Wang ◽  
Jingwen Ma ◽  
Xiaojie Li ◽  
Yang Li ◽  
Guoliang Zhang ◽  
...  
Keyword(s):  
Visible Light ◽  
Pore Size ◽  
Internal Diffusion ◽  
Henry Reaction ◽  
Diffusion Capacity ◽  
Small Pore Size ◽  
Large Pore ◽  
Small Pore ◽  
Large Pore Size

Mesoporous Cu2O spheres with a large pore size (LP-Cu2O) bring out a better performance towards the photocatalytic aza-Henry reaction than Cu2O spheres with a small pore size (SP-Cu2O).

Large Pore Size Nanoporous Materials from the Self-Assembly of Asymmetric Bottlebrush Block Copolymers

Nano Letters ◽  
2011 ◽  
Vol 11 (3) ◽  
pp. 998-1001 ◽  
Author(s):  
Justin Bolton ◽  
Travis S. Bailey ◽  
Javid Rzayev
Keyword(s):  
Block Copolymers ◽  
Pore Size ◽  
Self Assembly ◽  
Nanoporous Materials ◽  
The Self ◽  
Large Pore ◽  
Large Pore Size

Gradient porosity and large pore size NiTi shape memory alloys

2007 ◽  
Vol 57 (11) ◽  
pp. 1020-1023 ◽  
Author(s):  
Y.P. Zhang ◽  
D.S. Li ◽  
X.P. Zhang
Keyword(s):  
Shape Memory Alloys ◽  
Shape Memory ◽  
Pore Size ◽  
Large Pore ◽  
Niti Shape Memory Alloys ◽  
Large Pore Size

scholarly journals SBA-15 with Crystalline Walls Produced via Thermal Treatment with the Alkali and Alkali Earth Metal Ions

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5270
Author(s):  
Sung Soo Park ◽  
Sang-Wook Chu ◽  
Liyi Shi ◽  
Shuai Yuan ◽  
Chang-Sik Ha
Keyword(s):  
Metal Ions ◽  
Pore Size ◽  
Metal Ion ◽  
Pore Diameter ◽  
Earth Metal ◽  
Silica Matrix ◽  
Alkali Earth Metal ◽  
Alkali Earth ◽  
Large Pore ◽  
Large Pore Size

Crystalline walled SBA-15 with large pore size were prepared using alkali and alkali earth metal ions (Na+, Li+, K+ and Ca2+). For this work, the ratios of alkali metal ions (Si/metal ion) ranged from 2.1 to 80, while the temperatures tested ranged from 500 to 700 °C. The SBA-15 prepared with Si/Na+ ratios ranging from 2.1 to 40 at 700 °C exhibited both cristobalite and quartz SiO2 structures in pore walls. When the Na+ amount increased (i.e., Si/Na increased from 80 to 40), the pore size was increased remarkably but the surface area and pore volume of the metal ion-based SBA-15 were decreased. When the SBA-15 prepared with Li+, K+ and Ca2+ ions (Si/metal ion = 40) was thermally treated at 700 °C, the crystalline SiO2 of quartz structure with large pore diameter (i.e., 802.5 Å) was observed for Ca+2 ion-based SBA-15, while no crystalline SiO2 structures were observed in pore walls for both the K+ and Li+ ions treated SBA-15. The crystalline SiO2 structures may be formed by the rearrangement of silica matrix when alkali or alkali earth metal ions are inserted into silica matrix at elevated temperature.

Selective dealkylation of alkyl polycyclic aromatic hydrocarbons towards innovative upgrading process of practical heavy oil

2021 ◽  
Author(s):  
Fumiya Nakano ◽  
Tomohide Goma ◽  
Satoshi Suganuma ◽  
Etsushi Tsuji ◽  
Naonobu Katada
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Pore Size ◽  
Aromatic Hydrocarbons ◽  
Heavy Oil ◽  
Acid Sites ◽  
Polycyclic Aromatics ◽  
Large Pore ◽  
Polycyclic Aromatic ◽  
Long Chain ◽  
Large Pore Size

A silica-monolayer loaded on alumina with weak Brønsted acid sites and large pore size can selectively dealkylate alkyl polycyclic aromatics to long-chain alkanes and polycyclic aromatics for production of chemicals and fuel.

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