ANTIOXIDANT ACTIVITIES FROM VARIOUS EXTRACTS OF DIFFERENT PARTS OF KELAKAI (STENOCHLAENA PALUSTRIS) GROWN IN CENTRAL KALIMANTAN - INDONESIA
<p>ABSTRACT<br />Objectives: The aims of this research were to determine antioxidant activity from various extracts of different parts of kelakai (Stenochlaena palustris<br />[Burm.f.] Bedd) using two antioxidant testing methods, which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power<br />(FRAP), and correlation of total phenolic contents (TPC), total flavonoid contents (TFC), and total carotenoid contents (TCC) with their inhibitory<br />concentration 50% (IC<br />50<br />) of DPPH and exhibitory concentration 50% (EC<br />50<br />) of FRAP.<br />Methods: Sample was extracted by reflux using different polarity solvents. The extracts were evaporated using vacuum rotary evaporator. Antioxidant<br />activities were tested using DPPH and FRAP assays, determination of TPC, TFC, and TCC was carried out by ultraviolet-visible spectrophotometry, and<br />correlation with their IC<br />50<br /> of DPPH and EC<br />50<br /> of FRAP capacities was analyzed by Pearson’s method.<br />Results: Ethanolic root extract of kelakai (S. palustris) had the lowest IC<br />50<br /> of DPPH scavenging activity 0.8 µg/ml and the lowest EC<br /> of FRAP capacity<br />5.4 µg/ml. Ethanolic kelakai root extract demonstrated the highest phenolic content, ethyl acetate young leaves extract had the highest flavonoid<br />content, and the highest carotenoid content was given by n-hexane root extract. There was significantly negative correlation between TPC in root<br />extract of kelakai with their IC<br />50<br /> of DPPH and EC<br />50<br /> of FRAP.<br />Conclusions: All different extracts of kelakai parts were categorized as very strong antioxidants by DPPH method. Phenolic compounds in kelakai<br />root extract were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant<br />activities of root kelakai extract.<br />Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Stenochlaena palustris, Young leaves, Old leaves, Root.<br />50</p>