CONSTITUENT AND ANTIHYPERURICEMIC ACTIVITY OF STELECHOCARPUS BURAHOL LEAVES SUBFRACTIONS
Objectives: The goal of this research was to evaluate antihyperuricemic activity of Stelechocarpus burahol leaves subfractions and isolate its chemical constituent of active subfraction.Methods: Ethyl acetate fraction from S. burahol extract was subfractionated by vacuum liquid chromatography and the active subfractions were further subfractionated by classic column chromatography using isocratic eluent followed by isolated the chemical constituent from active subfraction. Hyperuricemic rat model was induced by given potassium oxonate intraperitoneally. The inhibitory effect of subfractions on the xanthine oxidase (XO) activity was determined using UV-visible spectrophotometry method.Results: Subfraction E.3, E.4 and E.5 significantly (p<0.05) reduced the serum uric acid level 43, 46 and 33,9%, respectively. The E.3, E.4 and E.5 have very weak activity. Subfraction E.3.2 and E.4.3 significantly (p<0.05) reduced the serum uric acid level 29 and 38 % respectively, however still very weak effect on XO activity. Chemical constituent which was isolated from E.4.3 subfraction was kaempferol-3-O-rhamnoside.Conclusion: The subfractions of ethyl acetate fraction had antihyperuricemic activity in vivo but less effect on XO acitivity in vitro. Compound R in active antihyperuricemic of subfraction E.4.3 was kaempferol-3-O-rhamnosideKeyword: Stelechocarpus burahol, subfraction, antihyperuricemic, xanthin oxidase inhibitory activity, kaempferol-3-O-rhamnoside.