scholarly journals A STUDY OF URINE FROM HIV-POSITIVE AND HIV-NEGATIVE INDIVIDUALS AT ANDHRA PRADESH

Author(s):  
Gundala Obulesu ◽  
Hanumanthappa Ar ◽  
Prabakar Reddy E

Objective: The aim of this study is to investigate the variations, if any, in urinary bacterial pathogens in HIV-positive and HIV-negative individuals.Methods: Urine sample, macroscopic and microscopic examination for identification of bacteria use by method of Standard loop method, using identification methods, Grams-staining, Biochemical tests.Results: Out of 75 HIV-positive males, 52 males were married, 23 males were unmarried; risk factors are sexually 28, occupationally majority were labor, 31, age group more in 19-25 age, only 9 HIV-positive patients presented with symptoms of urinary tract infection, Escherichia coli was the most frequently occurring bacterial isolate from positive urine culture followed by Klebsiella spp.Conclusion: The study period of 1 year and 8 months revealed that it is difficult to locate/identify an HIV-positive person in a place like Kadapa on account of confidentiality/lack of awareness among the population. Having identified the HIV-positive cases, collection of urine samples from the patients also needed counseling and intervention by the respective clinicians, especially when patients were not having any symptoms. Out of 50 HIV-negative individuals, only 1 (2%) urine sample is showing significant bacteriuria.

Author(s):  
Gundala Obulesu ◽  
Hanumanthappa Ar ◽  
Prabakar Reddy E

 Objective: The objective of this study is to investigate the variations, if any, in the types of intestinal parasites in HIV-positive and HIV-negative individuals.Methods: For their HIV status in our laboratory by enzyme-linked immunoasorbant assay and TRIDOT, sample stool, macroscopic and microscopic examination, formal-ether concentration technique f, saline wet mount, iodine wet mount, modified acid-fast stain. Results: For the purpose of statistical analysis, the enteric parasites are divided into the coccidian group (Cryptosporidium+Isospora belli) and conventional enteric parasites (Entamoeba histolytica, Giardia intestinalis, and Ascaris lumbricoides), and enteric parasitic infection is more common in HIV-positive patients with diarrhea than HIV-positive patient without diarrhea or HIV-negative individuals.Conclusion: The study period of 1 year and 8 months revealed that it is difficult to locate/identify a HIV-positive person in a place like Kadapa on account of confidentiality/lack of awareness among the population. Detection of intestinal parasites is 17.3% in HIV-positive patients and 12% in HIV-negative individuals.


Author(s):  
Gundala Obulesu ◽  
Hanumanthappa Ar ◽  
Prabakar Reddy E

 Objective: The objective of this study is to investigate the variations, if any, in the types of intestinal parasites in HIV-positive and HIV-negative individuals.Methods: For their HIV status in our laboratory by enzyme-linked immunoasorbant assay and TRIDOT, sample stool, macroscopic and microscopic examination, formal-ether concentration technique f, saline wet mount, iodine wet mount, modified acid-fast stain. Results: For the purpose of statistical analysis, the enteric parasites are divided into the coccidian group (Cryptosporidium+Isospora belli) and conventional enteric parasites (Entamoeba histolytica, Giardia intestinalis, and Ascaris lumbricoides), and enteric parasitic infection is more common in HIV-positive patients with diarrhea than HIV-positive patient without diarrhea or HIV-negative individuals.Conclusion: The study period of 1 year and 8 months revealed that it is difficult to locate/identify a HIV-positive person in a place like Kadapa on account of confidentiality/lack of awareness among the population. Detection of intestinal parasites is 17.3% in HIV-positive patients and 12% in HIV-negative individuals.


2020 ◽  
Vol 7 (3) ◽  
pp. 674
Author(s):  
Kagitha Pratap Gowd ◽  
Guntur Sai Ram ◽  
Gangadhar B. Belavadi

Background: It is well recognized that many fetomaternal and neonatal conditions are associated with thrombocytopenia. Study aimed to establish the possible etiology of children presenting with thrombocytopenia.Methods: The study was carried out in 100 children 1 month-12 years, admitted at department of paediatrics, Narayana Medical College and Hospital, Nellore, Andhra Pradesh, India with fever and thrombocytopenia. A thorough history was obtained and a general and systemic examination done. Routine investigations were done in all cases and specific investigations as and when required.Results: Total 100 cases were studied. Among them, 65% developed only fever, 10% developed fever with bleeding and 25% developed fever with shock. In total admissions 55% of children admitted with warning symptoms including abdominal pain, vomiting, reduced urine output and black colored stools. There is a significant influence of warning symptoms in predicting the outcome of fever with thrombocytopenia. 30% of children admitted with respiratory distress. 30% of children admitted with abdominal distension. 60% children admitted with hepatomegaly. In these children, 20% of them developed fever with shock and 13% of them developed fever with bleeding, 52% children admitted with platelet count less than 1 lakh. In these patients, 19% of them developed fever with shock and 9% of them developed fever with bleeding. 60% had dengue serology positive, 12% had positive Widal test, 8% children had positive urine culture and sensitivity. There was a significant influence of etiology in predicting the morbidity as 15% of children having positive serology for dengue developed fever with shock. 10% of children having positive serology for dengue developed fever with bleeding. Bleeding manifestations were seen most commonly in children with a platelet count less than 50000/μl.Conclusions: The commonest cause of febrile thrombocytopenia in this study was dengue fever in Children. Platelet count was the predictive of bleeding manifestations.


2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
EIY Madela ◽  
Alain Assounga

Abstract Background and Aims Urinary tract infection is among the most common causes of sepsis presenting in hospitals. The aim was to collect data to enable empirical treatment of urinary tract infections in HIV negative and positive nephrology patients while waiting for urine culture results in order to reduce hospital stay. Our aim is also to assess incidence and antimicrobial susceptibility of urinary tract infections in HIV negative and positive patients. Method A retrospective chart review of nephrology patients admitted from January to December 2014 in Nephrology ward and the first consecutive 200 outpatients seen in Nephrology clinic in 2014 at Inkosi Albert Luthuli Central Hospital was conducted. Information was gathered with the use of a data collection sheet and urinary tract infection was based on urine culture results. All data was analysed using Statistical Package for Social Sciences version 23. Percentages of basic characteristics were calculated between groups. Logistic regression analysis was used to identify factors associated with positive urine culture. Results There were 654 patients in the study, 514 (79%) were HIV negative and 139 (21%) were HIV positive. The incidence of UTI in nephrology patients was 9%, 10.1% in inpatients and 6.5% in outpatients. 22% were HIV positive (p value 0.883, 95% CI 0.550-2.003). 19% had Diabetes mellitus, 15% had Systemic Lupus Erythematosus and 5% were post renal transplant patients. Escherichia coli and Klebsiella pneumonia were the common causes of urinary tract infection at 40.7% and 15.3% respectively with 22% cases on extended beta lactam resistance. Conclusion There was no statistically significant difference on the incidence and anti-microbial isolates between HIV infected and HIV negative nephrology patients with urinary tract infection. Hence, empirical treatment for UTI should remain the same in the 2 groups. Escherichia coli and Klebsiella pneumoniae were the most commonly cultured organisms in both groups. There is microbial resistance to commonly used antibiotics. Constant assessment of anti-microbial sensitivity of urinary tract infections is of paramount importance.


2018 ◽  
Vol 2 (S1) ◽  
pp. e000136
Author(s):  
Suhana Jotva ◽  
Hemani Desai ◽  
Hansa Goswami

Aims and Objectives: The aim of present study is to estimate the frequency of abnormal PAP’s smears and mainly to detect precancerous and cancerous lesions as well as inflammatory lesions in HIV infected women. Methodology: Our study was a retrospective study of total 130 cases and PAP’s smears were examined in cytology section in Department of Pathology, BJ Medical College, Ahmedabad from 1st March 2017 to 31st August 2017. Both HIV positive and HIV negative patients were included in the study. 80 patients were HIV negative and 50 patients were HIV positive. The clinical history and relevant parameters were noted. All the smears were processed by a conventional method using Papanicolaou stain.  Results: Out of 130 cases, 118 cases were reported negative for intraepithelial lesions or malignancy (NILM). Five cases were positive for squamous intraepithelial lesions (SIL) out of which four were HIV positive. Seven cases were of atypical squamous cells of undetermined significance (ASCUS) out of which 5 were HIV positive. Three cases were showing bacterial vaginosis. 1 case was showing Trichomonas Vaginalis. 19 cases were showing changes of non-specific inflammation. 6 cases were showing atrophic changes.  Conclusion: HIV infected women are at more risk to develop cervical cytological abnormalities. The study revealed a maximum number of non-specific inflammatory smears followed by smears showing atrophic changes. Smears suspicious for malignancy (ASCUS) and smears showing squamous intraepithelial lesions (SIL) were more common in HIV positive patients. Thus regular PAP’s smears screening as recommended by National AIDS Control Organization (NACO) will help in early detection of cervical abnormalities in HIV positive women. 


2019 ◽  
Vol 9 (2) ◽  
pp. 70-72
Author(s):  
Satish Bagewadi ◽  
◽  
Umadevi Angadi ◽  
Roopali Shinde ◽  
◽  
...  

Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Samaneh Vaziri-Amjad ◽  
Mohammad Taghi Goodarzi ◽  
Jalal Poorolajal

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