scholarly journals STUDY OF PARMELIA PERLATA FOR ITS POTENTIAL AS ANTI-INFLAMMATORY AND ANTIARTHRITIC AGENT USING IN VITRO MODEL

2019 ◽  
Vol 12 (1) ◽  
pp. 95 ◽  
Author(s):  
Yogesh Diwakar ◽  
Chitra V ◽  
Evelyn Sharon S
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Maximum Effect ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Vitro Model

Objective: The objective of this study was to evaluate the anti-inflammatory and antiarthritic potential of Parmelia perlata. Methods: The relative study is based on in vitro anti-inflammatory and antiarthritic activity using hydroalcoholic extract of P. perlata (HAEPP). The preliminary phytochemical tests showed the presence of various phytochemical compounds such as alkaloids, flavonoids, and glycosides since the lichen species of P. perlata has the folklore claim of anti-inflammatory activity, thus it was studied by human red blood cells membrane stabilization method, and arthritic activity was carried using protein denaturation method using diclofenac as a standard.Results: The results showed eminent anti-inflammatory and antiarthritic activity in a dose-dependent manner. The membrane stabilization showed the maximum effect at 78.54% at the concentration of 1000 μg/, and the protein denaturation was also found maximum at 1000 μg/ml concentration at 79.43%. Thus, our research states the potent anti-inflammatory activity and antiarthritic effect in P. perlata. Conclusion: The HEAPP has a potent anti-inflammatory activity and antiarthritic activity. A further study has to be conducted to establish the pharmacological evidence behind the compound and the mechanism of action of the HAEPP on the inhibition of the inflammation process.

scholarly journals STUDY OF PARMELIA PERLATA FOR ITS POTENTIAL AS ANTI-INFLAMMATORY AND ANTIARTHRITIC AGENT USING IN VITRO MODEL

2019 ◽  
Vol 12 (1) ◽  
pp. 95
Author(s):  
Yogesh Diwakar ◽  
Chitra V ◽  
Evelyn Sharon S
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Maximum Effect ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Vitro Model

Objective: The objective of this study was to evaluate the anti-inflammatory and antiarthritic potential of Parmelia perlata. Methods: The relative study is based on in vitro anti-inflammatory and antiarthritic activity using hydroalcoholic extract of P. perlata (HAEPP). The preliminary phytochemical tests showed the presence of various phytochemical compounds such as alkaloids, flavonoids, and glycosides since the lichen species of P. perlata has the folklore claim of anti-inflammatory activity, thus it was studied by human red blood cells membrane stabilization method, and arthritic activity was carried using protein denaturation method using diclofenac as a standard.Results: The results showed eminent anti-inflammatory and antiarthritic activity in a dose-dependent manner. The membrane stabilization showed the maximum effect at 78.54% at the concentration of 1000 μg/, and the protein denaturation was also found maximum at 1000 μg/ml concentration at 79.43%. Thus, our research states the potent anti-inflammatory activity and antiarthritic effect in P. perlata. Conclusion: The HEAPP has a potent anti-inflammatory activity and antiarthritic activity. A further study has to be conducted to establish the pharmacological evidence behind the compound and the mechanism of action of the HAEPP on the inhibition of the inflammation process.

IN VITRO ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF PYRENACANTHA VOLUBILIS (EEPV)

2020 ◽  
pp. 156-158
Author(s):  
ANJALI P ◽  
VIMALAVATHINI R ◽  
KAVIMANI S
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Human Red Blood Cells ◽  
Anti Inflammatory ◽  
Dose Dependent

Objectives: The study was undertaken to evaluate the in vitro anti-inflammatory and anti-arthritic activity of the ethanolic extract of leaves of Pyrenacantha volubilis (EEPV) using human red blood cells (HRBCs) membrane stabilization and protein denaturation methods. Methods: In the present study, the in vitro anti-inflammatory and anti-arthritic activity of EEPV was carried out using HRBC membrane stabilization by hypotonicity-induced hemolysis and protein denaturation using egg albumin methods at various concentrations (100, 200, 400, 800, and 1000) of EEPV. Diclofenac sodium was used as reference standard. Results: P. volubilis was effective in inhibiting HRBC membrane stabilization and protein denaturation in a dose-dependent manner and was comparable to the standard drug diclofenac sodium. Conclusion: The study suggests that P. volubilis has potential anti-inflammatory and anti-arthritic activity.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS

2019 ◽  
pp. 278-281
Author(s):  
RAJESH A ◽  
DOSS A ◽  
TRESINA PS ◽  
MOHAN VR
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Inflammatory Agent ◽  
Potential Source ◽  
Anti Inflammatory

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action. Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin. Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml. Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

scholarly journals In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)

2018 ◽  
Vol 5 (10) ◽  
pp. 417-426 ◽  
Author(s):  
Samanjit Kaur ◽  
M. Syed Ali ◽  
V. Anuradha ◽  
V. Suganya ◽  
A. Ashashalini ◽  
...  
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Rhizophora Mucronata ◽  
Membrane Stabilization ◽  
In Vivo Models ◽  
Maximum Inhibition ◽  
Anti Inflammatory

In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.

Synthesis, Anti-Arthritic, and Anti-Inflammatory Activity of N-Tosyl aza Cyclophanes

2012 ◽  
Vol 65 (2) ◽  
pp. 186 ◽  
Author(s):  
Perumal Rajakumar ◽  
Ramar Padmanabhan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blood Cells ◽  
Bovine Serum ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Anti Inflammatory

The synthesis of novel N-tosyl tetraaza cyclophanes and N-tosyl diaza cyclophane incorporating m-terphenyl as spacer units is described. Anti-arthritic activity was studied by inhibition of the protein denaturation method (bovine serum albumin). All the N-tosyl aza cyclophanes exhibit excellent anti-arthritic activity. Anti-inflammatory activity of the synthesized cyclophanes was investigated using the human red blood cells (HRBC) membrane stabilization method and some of the N-tosyl aza cyclophanes exhibited good anti-inflammatory activity.

scholarly journals THE UNEXPLORED ROLES OF PROBIOTIC BACTERIA: IN VITRO ANTI-INFLAMMATORY AND ANTHELMINTIC ACTIVITY OF ENTEROCOCCUS FAECIUM BM10 KY788342 AND LACTOBACILLUS CASEI GM10 KY794586

2017 ◽  
Vol 10 (9) ◽  
pp. 117
Author(s):  
Neha Jain ◽  
Archana Mehta
Keyword(s):  
Enterococcus Faecium ◽  
Maximum Concentration ◽  
Lactobacillus Casei ◽  
Protein Denaturation ◽  
Anthelmintic Activity ◽  
Inflammatory Activity ◽  
Probiotic Strains ◽  
Anti Inflammatory ◽  
Vitro Model

  Objective: The aim of the present study was to evaluate the in vitro anti-inflammation activity and anthelmintic potential of two novel isolated probiotic strains through Enterococcus faecium BM10 KY788342 and Lactobacillus casei GM10 KY794586.Methods: In vitro anti-inflammatory activity was evaluated using protein denaturation inhibition method. Pheretima posthuma was used as a suitable in vitro model, and time of paralysis and death were used as parameters to evaluate anthelmintic potential of probiotic strains.Results: Lyophilized solutions of L. casei GM10 showed significant protein denaturation inhibition (56.20±0.86%) followed by E. faecium BM10 (52.28±0.31%) comparable to diclofenac (93.62±1.39%) at the maximum concentration of 250 μg/ml. Intracellular cell-free extract of E. faecium BM10 showed a strong anthelmintic (vermicidal) activity (6±0.23 minutes), followed by L. casei GM10 (9±0.05 minutes) comparable to piperazine citrate (20±0.422 minutes) and albendazole (24±0.43 minutes) at the maximum concentration of 100 mg/ml.Conclusion: Results of the present study concluded that both tested lactic acid bacteria strains exhibited significant in vitro anti-inflammatory activity and can be used as potent and safe anthelmintic agent.

scholarly journals Assessment of In vitro Anti-inflammatory Activity of Ginger and Diclofenac sodium combination

2020 ◽  
pp. 442-447
Author(s):  
Mousmi D. Thakur ◽  
Navin R. Sheth ◽  
Mihir K. Raval
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Research Work ◽  
Pharmacological Action ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Ginger Extract ◽  
Anti Inflammatory

The present research work aimed at evaluating the anti-inflammatory activity of Zingiber officinalis with Diclofenac sodium by HRBC membrane stabilization & Protein denaturation. The precluding of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization at different concentrations was performed for methanolic, hydro-methanolic ginger extract and diclofenac sodium. At a dose of 50µg/ml the maximum membrane stabilization 86.34% was found for Ginger extract(test) and at a dose of 500 mcg/ml membrane stabilization was found 91.16% for diclofenac sodium(standard) and the membrane stabilization for combination (ginger with diclofenac sodium) at a dose of 50µg/ml was recorded 86.43%, as the concentration increase(1000 mcg/ml) for combination(ginger with diclofenac sodium) the percentage protection was decreased. In vitro protein denaturation was performed by using egg albumin method. Maximum inhibition was observed in case of methanolic extract of ginger at concentration 1000mcg/ml and it was 78.83±5.17 and in hydro methanolic extract for Diclofenac sodium at concentration 1000mcg/ml and it was 63.37±2.78.Minimum inhibition observed in combination of methanolic extract of ginger and diclofenac sodium at concentration 1000mcg/ml and it was 25.27±1.76 and in combination of hydro-methanolic extract of ginger and Diclofenac sodium at concentration 1000mcg/ml and it was 28.23±3.14. The results of this study divulge that low dose combination of ginger and diclofenac sodium has higher anti-inflammatory activity than diclofenac sodium and ginger alone. With this initial study, research work could be extended further; therefore, the particular pharmacological action for the combination of ginger with diclofenac sodium could be discovered.

scholarly journals IN VITRO ANTI-INFLAMMATORY ACTIVITY OF SYRINGIC ACID

2018 ◽  
pp. 71-73 ◽  
Author(s):  
Shilpee Chanda ◽  
Archana R. Juvekar
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Syringic Acid ◽  
Inflammatory Activity ◽  
Proteinase Activity ◽  
Membrane Stabilization ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Proteinase Inhibition

Objective: The present study was carried out to investigate the in vitro anti-inflammatory activity of syringic acid (SA).  Methods: SA was tested for it's in vitro anti-inflammatory activity at different concentrations in protein denaturation, proteinase inhibition and human red blood cell (HRBC) membrane stabilization assay. The reference drugs used were aspirin and diclofenac sodium. Results: SA showed concentration-dependent inhibition of protein denaturation and proteinase activity with a half-maximal inhibitory concentration (IC50) value of 49.38±0.56 µg/ml and 53.73±0.27 µg/ml respectively. Heat-induced haemolysis was inhibited by SA with an IC50 value of 57.13±0.24 µg/ml. SA also inhibited the hypotonicity-induced haemolysis (IC50 value of 53.87±0.72 µg/ml). Conclusion: From the present study, we can conclude that SA possesses appreciable anti-inflammatory effect against denaturation of proteins, proteinase activity, and human red blood membrane stabilization assays. Further studies are required for determining the possible mechanisms behind its anti-inflammatory action.

scholarly journals In-vitro anti-inflammatory activity and anti-arthritic activity of ethyl acetate extracts of Skimmia anquetilia leaves

2020 ◽  
pp. 42-44
Author(s):  
Poonam Verma ◽  
Baljinder Singh ◽  
Amarjit Kaur ◽  
Vijender Kumar
Keyword(s):  
Ethyl Acetate ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Maximum Effect ◽  
Membrane Stabilization ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Anti Inflammatory ◽  
Dose Dependent

Current investigations were carried out for the validation of in-vitro anti-inflammatory and anti-arthritic property of leaves of Skimmia anquetilia using red blood cells membrane stabilization and protein denaturation methods respectively. Defatted ethylacetate extracts at different concentration levels (50, 100, 200 and 400 mg/ml) were used in these studies. Dose dependent inhibition of protein denaturation was found 92.41% at 400 mg/ml of extracts and 96.21 % at 100 mg/ml of acetyl salicylic acid as standard in antiarthritic study. Similarly, in membrane stabilization methods, maximum effect found 90.70 % at 400 mg/ml of extracts and 94.88 % at 100 mg/ml of diclofenac sodium as standard for anti-inflammatory evaluation. The results concluded that, ethyl acetate extract of S. anquetilia leaves has shown significant (*aP<0.05) anti-inflammatory and anti-arthritic effects.

scholarly journals Synthesis, Characterization and Evaluation of Anti-inflammatory Activity of Novel Indoline Derivatives

2016 ◽  
Vol 12 (12) ◽  
pp. 4557-4563
Author(s):  
Dhinesh kumar Manoharan
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Potent Inhibition ◽  
Ft Ir ◽  
Anti Inflammatory

Series of indoline derivatives were synthesized using N-(4-aminophenyl) indoline-1-carbothiamide as a precursor. The structures of synthesized compounds were confirmed by   FT-IR, 1H-NMR, 13C-NMR and LC-MS. The in vitro anti-inflammatory activity of synthesized indoline derivatives were examined by standard anti-denaturation assay. The compounds 4a (IC50 = 62.2 µg/ml) and 4b (IC50 = 60.7 µg/ml) showed potent inhibition on protein denaturation. The compounds 5a (IC50 = 97.8 µg/ml) exhibits moderate inhibition on protein denaturation

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