ANTIBACTERIAL ACTIVITY OF SYNTHESIZED SILVER NANOPARTICLES BY SIMAROUBAGLAUCA AGAINST PATHOGENIC BACTERIA

Objective: The present study outline the plant-mediated synthesis of silver nanoparticles (AgNPs) using leaf extract Simaroubaglauca, which act as both reducing and stabilizing agent.Methods: Formation of silver nanoparticles was confirmed by primarily by Ultraviolet/visible spectroscopy. X-ray diffraction studies revealed the crystallinity of the nanoparticles. The scanning electron microscopy was carried out to determine the mean particle size, as well as the morphology of the NPs and the composition of elements, was studied with Energy Dispersive X-ray analysis (EDS).Results: The silver nanoparticles were spherical in shape with a mean size of 23 nm. The EDS showed strong optical absorption peak at 3keV and it was confirmed the formation of AgNPs. The synthesised AgNPs further utilized for the evaluation of antibacterial activity and shown significant antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Enterobacter and Klebsiella pneumonia at 50 µg/ml and 100µg/ml concentrations.Conclusion: The synthesised silver nanoparticles have been characterised by UV-vis, SEM-EDAX and XRD to determine the sizes and shapes of the silver nanoparticles.

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GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES OF A MARINE RED ALGA SPYRIDIA FUSIFORMIS AND THEIR ANTIBACTERIAL ACTIVITY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.17105 ◽

2017 ◽

Vol 9 (5) ◽

pp. 192 ◽

Cited By ~ 7

Author(s):

Subbiah Murugesan ◽

Sundaresan Bhuvaneswari ◽

Vajiravelu Sivamurugan

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Red Alga ◽

Diffusion Method ◽

Present System ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Uv Visible

Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out.Methods: The seaweed extract was used for the synthesis of AgNPs at room temperature. The silver nanoparticles were characterized by using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

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In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

Bioinorganic Chemistry and Applications ◽

10.1155/2014/581890 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 61

Author(s):

S. Rajeshkumar ◽

C. Malarkodi

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Food Safety ◽

Pathogenic Bacteria ◽

Face Centered Cubic ◽

X Ray Diffraction ◽

X Ray ◽

Food Borne ◽

Uv Visible

Biosynthesis of silver nanoparticles usingPlanomicrobiumsp. and to explore the antibacterial activity against food borne pathogenic bacteriaBacillus subtilis, (3053)Klebsiella planticola(2727)Klebsiella pneumoniae(MAA)Serratia nematodiphila(CAA) andEscherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL ofPlanomicrobiumsp. culture supernatant. The bioreduction of pure AgNO3was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.

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Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.38.35 ◽

2014 ◽

Vol 38 ◽

pp. 35-45 ◽

Cited By ~ 2

Author(s):

B. Anandh ◽

A. Muthuvel ◽

M. Emayavaramban

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Face Centered Cubic ◽

Lagenaria Siceraria ◽

Human Pathogens ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Ft Ir ◽

Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

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Antibacterial Activity of Ag-Doped TiO2 and Ag-Doped ZnO Nanoparticles

International Journal of Photoenergy ◽

10.1155/2018/5927485 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 19

Author(s):

Gebretinsae Yeabyo Nigussie ◽

Gebrekidan Mebrahtu Tesfamariam ◽

Berhanu Menasbo Tegegne ◽

Yemane Araya Weldemichel ◽

Tesfakiros Woldu Gebreab ◽

...

Keyword(s):

Antibacterial Activity ◽

Zno Nanoparticles ◽

Pathogenic Bacteria ◽

Anatase Phase ◽

Sol Gel ◽

X Ray Diffraction ◽

Zno Nps ◽

Sem Images ◽

X Ray ◽

Doped Zno

We report in this paper antibacterial activity of Ag-doped TiO2 and Ag-doped ZnO nanoparticles (NPs) under visible light irradiation synthesized by using a sol-gel method. Structural, morphological, and basic optical properties of these samples were investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectrum, and UV-Vis reflectance. Room temperature X-ray diffraction analysis revealed that Ag-doped TiO2 has both rutile and anatase phases, but TiO2 NPs only have the anatase phase. In both ZnO and Ag-doped ZnO NPs, the hexagonal wurtzite structure was observed. The morphologies of TiO2 and ZnO were influenced by doping with Ag, as shown from the SEM images. EDX confirms that the samples are composed of Zn, Ti, Ag, and O elements. UV-Vis reflectance results show decreased band gap energy of Ag-doped TiO2 and Ag-doped ZnO NPs in comparison to that of TiO2 and ZnO. Pathogenic bacteria, such as Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, were used to assess the antibacterial activity of the synthesized materials. The reduction in the viability of all the three bacteria to zero using Ag-doped ZnO occurred at 60 μg/mL of culture, while Ag-doped TiO2 showed zero viability at 80 μg/mL. Doping of Ag on ZnO and TiO2 plays a vital role in the increased antibacterial activity performance.

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Synthesis and Characterization of Surface Protected Nanocrystalline Particles of Titania

MRS Proceedings ◽

10.1557/proc-519-329 ◽

1998 ◽

Vol 519 ◽

Cited By ~ 1

Author(s):

E. Scolan ◽

C. Sanchez

Keyword(s):

X Ray Diffraction ◽

Elastic Light Scattering ◽

Titanium Butoxide ◽

X Ray ◽

Toluene Sulfonic Acid ◽

Nanocrystalline Particles ◽

Mean Size ◽

The Mean ◽

Cp Mas Nmr

AbstractMonodisperse non-aggregated nanoparticles of titania are obtained through hydrolysis at 60°C of titanium butoxide in the presence of acetylacetone and para-toluene sulfonic acid. After drying the resulting xerosols can be dispersed without aggregation in water-alcoholic or alcoholic solutions. The characterizations of the nanoparticles have been carried out by using quasi-elastic light scattering (QELS), 13C 1H in solution and X-ray diffraction, TEM, TG-DTA, 13C CP-MAS NMR in the solid state. The mean size of the anatase oxide core can be adjusted in the I to 5 nanometer range by a careful tuning of the synthetic conditions. The anatase particles are surrounded by acetylacetonato ligands, para-toluene sulfonate based species and water.

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Biosynthesis of silver nanoparticles using lavender leaf and their applications for catalytic, sensing, and antioxidant activities

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0041 ◽

2016 ◽

Vol 5 (6) ◽

Cited By ~ 11

Author(s):

Brajesh Kumar ◽

Kumari Smita ◽

Luis Cumbal

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Antioxidant Activities ◽

Present Report ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Transmission Electron ◽

Red Color ◽

Uv Visible

AbstractThe present report summarizes an eco-friendly approach for the biosynthesis of silver nanoparticles (AgNPs) using the leaf extract of lavender. Initially, the synthesis of AgNPs was visually observed by the appearance of a wine red color. The optical property, morphology, and structure of as-synthesized AgNPs were characterized by UV-visible spectroscopy, dynamic light scattering, transmission electron microscopy, and X-ray diffraction analyses. All characterization data revealed the formation of crystalline and spherical AgNPs (Ag/Ag

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A Facile Method for the Synthesis of Silver Nanoparticles in the Presence of Sodium Phosphate

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.109.174 ◽

2011 ◽

Vol 109 ◽

pp. 174-177 ◽

Cited By ~ 2

Author(s):

Yu Li Shi ◽

Qi Zhou ◽

Li Yun Lv ◽

Wang Hong

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Sodium Phosphate ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Stabilizing Agents ◽

Transmission Electron ◽

Silver Nps ◽

Uv Visible

A facile method for the synthesis of silver nanoparticles (NPs) has been developed by using sodium phosphate (Na3PO4) as stabilizing agents and glucose the reducing agent, respectively. The obtained silver NPs have been characterized by X-ray diffraction (XRD), UV-visible spectroscopy (UV-vis) and transmission electron microscopy (TEM). It was found that in the presence of sodium phosphate, silver NPs with different morphologies and sizes were obtained. The formation mechanism of diverse silver NPs was studied preliminarily.

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Synthesis, Characterization, and Antibacterial Activity of Ag2O-Loaded Polyethylene Terephthalate Fabric via Ultrasonic Method

Nanomaterials ◽

10.3390/nano9030450 ◽

2019 ◽

Vol 9 (3) ◽

pp. 450 ◽

Cited By ~ 6

Author(s):

Armin Rajabi ◽

Mariyam Ghazali ◽

Ebrahim Mahmoudi ◽

Amir Baghdadi ◽

Abdul Mohammad ◽

...

Keyword(s):

Antibacterial Activity ◽

Polyethylene Terephthalate ◽

Ultrasonic Method ◽

Antibacterial Properties ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Key Factor ◽

Effects Of Ph ◽

Testing Field

In this study, Ag2O was synthesized on polyethylene terephthalate fabrics by using an ultrasonic technique with Ag ion reduction in an aqueous solution. The effects of pH on the microstructure and antibacterial properties of the fabrics were evaluated. X-ray diffraction confirmed the presence of Ag2O on the fabrics. The fabrics were characterized by Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, and wettability testing. Field-emission scanning electron microscopy verified that the change of pH altered the microstructure of the materials. Moreover, the antibacterial activity of the fabrics against Escherichia coli was related to the morphology of Ag2O particles. Thus, the surface structure of Ag2O particles may be a key factor of the antibacterial activity.

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Actinobacterial-Mediated Fabrication of Silver Nanoparticles and Their Broad Spectrum Antibacterial Activity Against Clinical Pathogens

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2020.17440 ◽

2020 ◽

Vol 20 (5) ◽

pp. 2902-2910 ◽

Cited By ~ 4

Author(s):

Poongazhalselvan Devagi ◽

Thirunavukarasu Chitrikha Suresh ◽

Revathi Vedachalam Sandhiya ◽

Mohan Sairandhry ◽

Selvaraj Bharathi ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Broad Spectrum ◽

Fourier Transforms ◽

Salmonella Typhi ◽

X Ray Diffraction ◽

Vibrio Cholera ◽

X Ray ◽

The Face ◽

Average Size

The aim of this study is to fabricate silver nanoparticles (AgNPs) using actinobacterial strain isolated from lawn soil. Among six isolates, one isolate named AS-3 was potent in AgNPs production; hence it was identified deployed on gene sequence (16S rRNA) as Streptomyces spongiicola AS-3 (99.8% similarity). Actinobacteria mediated synthesized AgNPs were analyzed using UV-visible spectroscopy (UV-Vis), which showed a Surface Plasmon Resonance (SPR) at around λ = 443 nm. Scanning electron microscopy (SEM) analyses revealed the occurrence of predominant spherical AgNPs with polydispersed, with an average size of 22 nm. Energy-dispersive X-ray spectroscopy (EDS) established the existence of silver component. While the Fourier transforms infrared spectroscopy (FTIR) evidenced the occurrence of proteins as the bio reduction and topping agents over the AgNPs. X-ray diffraction (XRD) examination confirmed the obtained AgNPs were in crystalline planes of the face centric cubic. The S. spongiicola AgNPs antibacterial activity showed a broad spectrum antibacterial action against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Vibrio cholera, Shigella sp., and Salmonella typhi were confirmed by disc diffusion test and MIC analysis.

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