scholarly journals In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
S. Rajeshkumar ◽  
C. Malarkodi
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Food Safety ◽  
Pathogenic Bacteria ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
X Ray ◽  
Food Borne ◽  
Uv Visible

Biosynthesis of silver nanoparticles usingPlanomicrobiumsp. and to explore the antibacterial activity against food borne pathogenic bacteriaBacillus subtilis, (3053)Klebsiella planticola(2727)Klebsiella pneumoniae(MAA)Serratia nematodiphila(CAA) andEscherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL ofPlanomicrobiumsp. culture supernatant. The bioreduction of pure AgNO3was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.

scholarly journals Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

2014 ◽  
Vol 38 ◽  
pp. 35-45 ◽  
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Face Centered Cubic ◽  
Lagenaria Siceraria ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

A Facile Method for the Synthesis of Silver Nanoparticles in the Presence of Sodium Phosphate

2011 ◽  
Vol 109 ◽  
pp. 174-177 ◽  
Author(s):  
Yu Li Shi ◽  
Qi Zhou ◽  
Li Yun Lv ◽  
Wang Hong
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Sodium Phosphate ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Stabilizing Agents ◽  
Transmission Electron ◽  
Silver Nps ◽  
Uv Visible

A facile method for the synthesis of silver nanoparticles (NPs) has been developed by using sodium phosphate (Na3PO4) as stabilizing agents and glucose the reducing agent, respectively. The obtained silver NPs have been characterized by X-ray diffraction (XRD), UV-visible spectroscopy (UV-vis) and transmission electron microscopy (TEM). It was found that in the presence of sodium phosphate, silver NPs with different morphologies and sizes were obtained. The formation mechanism of diverse silver NPs was studied preliminarily.

GC/MS Analysis and Phyto-synthesis of Silver Nanoparticles Using Amygdalus spinosissima Extract: Antibacterial, Antioxidant Effects, Anticancer and Apoptotic Effects

2022 ◽  
Author(s):  
Azadeh Farmahini Farahani ◽  
Seyed Mohammad Mahdi Hamdi ◽  
Amir Mirzaee
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Cell Line ◽  
Face Centered Cubic ◽  
X Ray ◽  
Ms Analysis ◽  
Uv Visible ◽  
Apoptotic Effects ◽  
Antioxidant Effects ◽  
Mcf 7

Background: The present study was aimed at phyto-synthesized silver nanoparticles (AgNPs) using Amygdalus spinosissima (A. spinosissima) extract and to investigate the antibacterial, antioxidant effects, anticancer and apoptotic effects of phyto-synthe-sized AgNPs. Methods: The bio-fabricated AgNPs were characterized using UV-visible spectroscopy (UV-visible), X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Energy Disper-sive X-ray (EDX). Results: The phyto-synthesized AgNPs showed maximum absorption in 438 nm, in the UV-visible spectrum. XRD peaks were observed at 2θ values in 38.20°, 44.40°, 64.60°, and 77.50° which are indexed as (111), (200), (220), and (311) bands of Face-Centered Cubic (FCC) structures of silver. FTIR analysis indicated that the AgNPs were capped with A. spinosissima extract. SEM and TEM micrographs revealed that the fabricated AgNPs were spherical and the average size range was 17.89 nm. Also, the EDX results show that the content of Ag was 90%. Conclusion: The phyto-synthesized AgNPs had significant antibacterial activity against Gram-negative bacteria, as well as, the AgNPs exhibited great inhibitory effects on DPPH radicals and their antioxidant properties were favorably comparable to the antioxidant outcomes of ascorbic acid. Moreover, the AgNPs showed anti-cancer activity against the MCF-7 cell line with the IC50=6.1 µg/ml. Moreover, the phyto-synthesized AgNPs could induce apoptosis in the MCF-7 cell line significantly. The GC-MS analysis of the A. spinosissima extract showed that 102 bioactive phyto-chemical compounds, which be of use to the synthesis of AgNPs.   

scholarly journals Antimicrobial Activity of Silver Nanoparticles Synthesized Using Goat Milk against Pathogens of Selected Vegetables

2019 ◽  
pp. 1-10 ◽  
Author(s):  
T. A. Ihum ◽  
C. C. Iheukwumere ◽  
I. O. Ogbonna ◽  
G. M. Gberikon
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Goat Milk ◽  
Cubic Phase ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron

This study was carried out to determine the antimicrobial activity of silver nanoparticles synthesized using goat milk against pathogens of selected vegetables. Synthesis of Silver nanoparticles was done using Goat milk, and characterized using Ultra Violet-Visible absorption spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X- ray diffraction (XRD) and Transmission Electron Microscopy (TEM). Maximum absorbance of Goat milk synthesized AgNPs was observed at 417 nm, with FTIR peaks at 3455 cm−1, 1628 cm−1, 1402 cm−1, 1081 cm−1 and 517 cm−1, indicating that proteins in Goat milk (GM) were the capping and stabilization molecules involved the synthesis of AgNPs. Transmission electron microscopy analysis showed that the biosynthesized particles were spherical in shape having a size of 10-100 nm, X- ray diffraction (XRD) pattern agreed with the crystalline nature and face-centered cubic phase of AgNPs. Evaluation of the antimicrobial activity of AgNPs synthesized using GM against the indicator strains (Staphylococcus aureus CIP 9973, Pectobacterium carotovorum Pec1, Enterobacter cloacae AS10, Klebsiella aerogenes OFM28, Proteus mirabilis UPMSD3 and Escherichia coli 2013C-3342) isolated from selected vegetables, was carried out using the Agar diffusion assay at different concentrations of 25, 75 and 100 µl/ml. The present study demonstrated that the AgNPs synthesized using Goat milk have potent biological activities, which can find applications in diverse areas.

scholarly journals GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES OF A MARINE RED ALGA SPYRIDIA FUSIFORMIS AND THEIR ANTIBACTERIAL ACTIVITY

2017 ◽  
Vol 9 (5) ◽  
pp. 192 ◽  
Author(s):  
Subbiah Murugesan ◽  
Sundaresan Bhuvaneswari ◽  
Vajiravelu Sivamurugan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Red Alga ◽  
Diffusion Method ◽  
Present System ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Uv Visible

Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out.Methods: The seaweed extract was used for the synthesis of AgNPs at room temperature. The silver nanoparticles were characterized by using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

scholarly journals Photo-Irradiated Biosynthesis of Silver Nanoparticles Using Edible MushroomPleurotus floridaand Their Antibacterial Activity Studies

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Ravishankar Bhat ◽  
Raghunandan Deshpande ◽  
Sharanabasava V. Ganachari ◽  
Do Sung Huh ◽  
A. Venkataraman
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
X Ray Diffraction ◽  
Spectroscopy Analysis ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Synthetic Procedure ◽  
Uv Visible

This is a report on photo-irradiated extracellular synthesis of silver nanoparticles using the aqueous extract of edible oyster mushroom (Pleurotus florida) as a reducing agent. The appearance, size, and shape of the silver nanoparticles are understood by UV-visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The X-ray diffraction studies, energy dispersive X-ray analysis indicate that particles are crystalline in nature. Fourier transform infrared spectroscopy analysis revealed that the nanoparticles are covered with biomoieties on their surface. As can be seen from our studies, the biofunctionalized silver nanoparticles thus produced have shown admirable antimicrobial effects, and the synthetic procedure involved is eco-friendly and simple, and hence high range production of the same can be considered for using them in many pharmaceutical applications.

scholarly journals Synthesis of silver nanoparticles using marine macroalgae Padina sp. and its antibacterial activity towards pathogenic bacteria

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Prakash Bhuyar ◽  
Mohd Hasbi Ab. Rahim ◽  
Sathyavathi Sundararaju ◽  
Rameshprabu Ramaraj ◽  
Gaanty Pragas Maniam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Marine Algae ◽  
Marine Alga ◽  
Ag Nps ◽  
X Ray ◽  
Uv Visible ◽  
Scanning Electron

Abstract Background Marine algae used as a food source for ocean life and range in color from red to green to brown grow along rocky shorelines around the world. The synthesis of silver nanoparticles by marine alga Padina sp. and its characterization were fulfilled by using UV-visible spectrophotometer, Fourier transform infrared spectroscopy, scanning electron microscopy and field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Results UV-visible absorption spectrum revealed that the formation of Ag nanoparticles was increased by the addition of marine algae and the spectral peak observed between a wavelength of ~ 420 nm and 445 nm. In addition, SEM and FESEM images examined the surface morphology and the size of the synthesized NPs was relatively uniform in size ~ 25–60 nm. Energy-dispersive X-ray spectroscopy analysis confirmed the purity of Ag NPs with atomic percentage of 48.34% Ag. The synthesized Ag NPs showed highly potent antibacterial activity. The Staphylococcus aureus and Pseudomonas aeruginosa were found to be more susceptible to silver nanoparticles by forming 15.17 ± 0.58 mm and 13.33 ± 0.76 mm of diameter of the inhibition zone, respectively. Conclusions The study suggested that marine alga Padina sp. could be an alternative source for the production of Ag nanoparticles and are efficient antimicrobial compounds against both gram-negative and gram-positive bacteria which can be a promising material against infectious bacteria.

scholarly journals ANTIBACTERIAL ACTIVITY OF SYNTHESIZED SILVER NANOPARTICLES BY SIMAROUBAGLAUCA AGAINST PATHOGENIC BACTERIA

2017 ◽  
Vol 9 (4) ◽  
pp. 19 ◽  
Author(s):  
C. P. Chandrappa ◽  
N. Chandrasekar ◽  
M. Govindappa ◽  
Chaitra Shanbhag ◽  
Uttam Kumar Singh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Klebsiella Pneumonia ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Optical Absorption Peak ◽  
Mean Size ◽  
The Mean

Objective: The present study outline the plant-mediated synthesis of silver nanoparticles (AgNPs) using leaf extract Simaroubaglauca, which act as both reducing and stabilizing agent.Methods: Formation of silver nanoparticles was confirmed by primarily by Ultraviolet/visible spectroscopy. X-ray diffraction studies revealed the crystallinity of the nanoparticles. The scanning electron microscopy was carried out to determine the mean particle size, as well as the morphology of the NPs and the composition of elements, was studied with Energy Dispersive X-ray analysis (EDS).Results: The silver nanoparticles were spherical in shape with a mean size of 23 nm. The EDS showed strong optical absorption peak at 3keV and it was confirmed the formation of AgNPs. The synthesised AgNPs further utilized for the evaluation of antibacterial activity and shown significant antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Enterobacter and Klebsiella pneumonia at 50 µg/ml and 100µg/ml concentrations.Conclusion: The synthesised silver nanoparticles have been characterised by UV-vis, SEM-EDAX and XRD to determine the sizes and shapes of the silver nanoparticles.

Green synthesis of silver nanoparticles of olea ferruginea root extract: Characterizations, biological assays and their catalytic application in dye degradation

2021 ◽  
pp. 1-13
Author(s):  
Iqbal Wadan ◽  
Haroon Khan ◽  
Kamran Tahir ◽  
Muhammad Khalid Khan ◽  
Barkat Ali Khan
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Dye Degradation ◽  
Root Extract ◽  
X Ray Diffraction ◽  
Visible Absorption ◽  
Biological Assays ◽  
X Ray ◽  
Transmission Electron ◽  
Uv Visible

In current work, silver nanoparticles (AgNPs) were prepared by a rapid biogenic technique “Green method” utilizing root extract of Olea ferruginea. The synthesized nanoparticles were characterized for UV visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning electron microscopy (SEM), High Resolution Transmission Electron Microscopy Studies (HRTEM) and biological assays. UV visible absorption spectroscopy confirms the formation of AgNPs by giving Surface Plasmon Resonance (SPR) peak at 430 nm. FTIR study showed occurrence of various functional groups present in root extract of Olea ferruginea. X-ray diffraction analysis determined the crystalline nature of bio-fabricated silver nanoparticles. The elemental composition of green made silver nanoparticles was studied through Energy Dispersive Spectroscopy (EDS) analysis. SEM & HRTEM study revealed the size, shape, surface morphology & dispersion level of molecules. The biologically synthesized AgNPs showed high antimicrobial, antifungal and antioxidant activity. The AgNPs are observed to be an excellent catalyst on reduction of hazardous dyes, which is confirmed by a decrease in absorbance of maximum values.

scholarly journals Decolorization of Methylene Blue by Ag/SrSnO3 Composites under Ultraviolet Radiation

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Patcharanan Junploy ◽  
Titipun Thongtem ◽  
Somchai Thongtem ◽  
Anukorn Phuruangrat
Keyword(s):  
Electron Microscopy ◽  
Methylene Blue ◽  
Uv Radiation ◽  
Photoelectron Spectroscopy ◽  
Separation Process ◽  
X Ray Diffraction ◽  
Electron Hole ◽  
X Ray ◽  
Negative Effect ◽  
Uv Visible

SrSn(OH)6 precursors synthesized by a cyclic microwave radiation (CMR) process were calcined at 900°C for 3 h to form rod-like SrSnO3. Further, the rod-like SrSnO3 and AgNO3 in ethylene glycol (EG) were ultrasonically vibrated to form rod-like Ag/SrSnO3 composites, characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), electron microscopy (EM), Fourier transform infrared (FTIR) spectroscopy, and UV-visible analysis. The photocatalyses of rod-like SrSnO3, 1 wt%, 5 wt%, and 10 wt% Ag/SrSnO3 composites were studied for degradation of methylene blue (MB, C16H18N3SCl) dye under ultraviolet (UV) radiation. In this research, the 5 wt% Ag/SrSnO3 composites showed the highest activity, enhanced by the electron-hole separation process. The photoactivity became lower by the excessive Ag nanoparticles due to the negative effect caused by reduction in the absorption of UV radiation.

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