scholarly journals Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

2014 ◽  
Vol 38 ◽  
pp. 35-45 ◽  
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Face Centered Cubic ◽  
Lagenaria Siceraria ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

Biosynthesis of silver nanoparticles using lavender leaf and their applications for catalytic, sensing, and antioxidant activities

2016 ◽  
Vol 5 (6) ◽  
Author(s):  
Brajesh Kumar ◽  
Kumari Smita ◽  
Luis Cumbal
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Present Report ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Transmission Electron ◽  
Red Color ◽  
Uv Visible

AbstractThe present report summarizes an eco-friendly approach for the biosynthesis of silver nanoparticles (AgNPs) using the leaf extract of lavender. Initially, the synthesis of AgNPs was visually observed by the appearance of a wine red color. The optical property, morphology, and structure of as-synthesized AgNPs were characterized by UV-visible spectroscopy, dynamic light scattering, transmission electron microscopy, and X-ray diffraction analyses. All characterization data revealed the formation of crystalline and spherical AgNPs (Ag/Ag

A Facile Method for the Synthesis of Silver Nanoparticles in the Presence of Sodium Phosphate

2011 ◽  
Vol 109 ◽  
pp. 174-177 ◽  
Author(s):  
Yu Li Shi ◽  
Qi Zhou ◽  
Li Yun Lv ◽  
Wang Hong
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Sodium Phosphate ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Stabilizing Agents ◽  
Transmission Electron ◽  
Silver Nps ◽  
Uv Visible

A facile method for the synthesis of silver nanoparticles (NPs) has been developed by using sodium phosphate (Na3PO4) as stabilizing agents and glucose the reducing agent, respectively. The obtained silver NPs have been characterized by X-ray diffraction (XRD), UV-visible spectroscopy (UV-vis) and transmission electron microscopy (TEM). It was found that in the presence of sodium phosphate, silver NPs with different morphologies and sizes were obtained. The formation mechanism of diverse silver NPs was studied preliminarily.

scholarly journals Phyto-Extract-Mediated Synthesis of Silver Nanoparticles Using Aqueous Extract of Sanvitalia procumbens, and Characterization, Optimization and Photocatalytic Degradation of Azo Dyes Orange G and Direct Blue-15

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6144
Author(s):  
Madeeha Aslam ◽  
Fozia Fozia ◽  
Anadil Gul ◽  
Ijaz Ahmad ◽  
Riaz Ullah ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Carboxylic Acid ◽  
Plant Extract ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Direct Blue ◽  
Orange G ◽  
Ft Ir ◽  
Uv Visible ◽  
Direct Blue 15

Green synthesis of silver nanoparticles (AgNPs) employing an aqueous plant extract has emerged as a viable eco-friendly method. The aim of the study was to synthesize AgNPs by using plant extract of Sanvitalia procumbens (creeping zinnia) in which the phytochemicals present in plant extract act as a stabilizing and reducing agent. For the stability of the synthesized AgNPs, different parameters like AgNO3 concentration, volume ratios of AgNO3, temperature, pH, and contact time were studied. Further, AgNPs were characterized by UV–visible spectroscopy, FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-ray Diffraction), SEM (Scanning Electron Microscopy), and EDX (Energy Dispersive X-ray Spectrometer) analysis. FT-IR analysis showed that the plant extract contained essential functional groups like O–H stretching of carboxylic acid, N–H stretching of secondary amides, and C–N stretching of aromatic amines, and C–O indicates the vibration of alcohol, ester, and carboxylic acid that facilitated in the green synthesis of AgNPs. The crystalline nature of synthesized AgNPs was confirmed by XRD, while the elemental composition of AgNPs was detected by energy dispersive X-ray analysis (EDX). SEM studies showed the mean particle diameter of silver nanoparticles. The synthesized AgNPs were used for photocatalytic degradation of Orange G and Direct blue-15 (OG and DB-15), which were analyzed by UV-visible spectroscopy. Maximum degradation percentage of OG and DB-15 azo dyes was observed, without any significant silver leaching, thereby signifying notable photocatalytic properties of AgNPs.

scholarly journals GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES OF A MARINE RED ALGA SPYRIDIA FUSIFORMIS AND THEIR ANTIBACTERIAL ACTIVITY

2017 ◽  
Vol 9 (5) ◽  
pp. 192 ◽  
Author(s):  
Subbiah Murugesan ◽  
Sundaresan Bhuvaneswari ◽  
Vajiravelu Sivamurugan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Red Alga ◽  
Diffusion Method ◽  
Present System ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Uv Visible

Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out.Methods: The seaweed extract was used for the synthesis of AgNPs at room temperature. The silver nanoparticles were characterized by using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

scholarly journals In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
S. Rajeshkumar ◽  
C. Malarkodi
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Food Safety ◽  
Pathogenic Bacteria ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
X Ray ◽  
Food Borne ◽  
Uv Visible

Biosynthesis of silver nanoparticles usingPlanomicrobiumsp. and to explore the antibacterial activity against food borne pathogenic bacteriaBacillus subtilis, (3053)Klebsiella planticola(2727)Klebsiella pneumoniae(MAA)Serratia nematodiphila(CAA) andEscherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL ofPlanomicrobiumsp. culture supernatant. The bioreduction of pure AgNO3was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.

scholarly journals Antibacterial and Antioxidant Studies of Green Synthesized Silver Nanoparticles using Azadirachta indica (Neem) Leaf Extract

2021 ◽  
Vol 9 (3) ◽  
pp. 220-226
Author(s):  
Bishow Regmi ◽  
Tirtha Raj Binadi ◽  
Sarb Narayan Jha ◽  
Rajib Kumar Chaudhary ◽  
Bhoj Raj Poudel ◽  
...  
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Average Particle Size ◽  
Diffusion Method ◽  
Average Particle ◽  
Face Centered Cubic ◽  
X Ray ◽  
Uv Visible

Silver nanoparticles (AgNPs) have been synthesized by green synthesis using Azadirachta indica leaf extract as both reducing and stabilizing agent. Synthesis of colloidal AgNPs was monitored by UV- visible spectroscopy. The UV- visible spectrum showed a peak at 455 nm corresponding to the plasmon absorbance of the silver nanoparticles. Crystallite structure of silver nanoparticles was studied using X-ray diffraction (XRD) analysis which revealed the face-centered cubic structure (FCC) with average particle size of 8.9 nm, calculated using Debye-Scherrer’s equation. Transmission electron microscopy (TEM) image revealed the agglomeration of small grain with particle size ranging from 2 to 14 nm. FCC crystalline nature was also evident from selected area electron diffraction (SAED) analysis. High purity of as-synthesized AgNPs was analyzed using energy dispersive X-ray (EDX) spectroscopy. Band gap energy was calculated to be 2.7 eV from UV- Visible spectra. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was stabilized by AgNPs which reveals its antioxidant efficacy. Well diffusion method showed 7 mm to 12 mm zone of inhibition (ZOI) against Gram-positive and Gram-negative bacteria, respectively confirming the antibacterial potential of AgNPs. Int. J. Appl. Sci. Biotechnol. Vol 9(3): 220-226.

Phytochemical Preparation, Characterization and Photocatalytic Applications of Ag-TiO2 Nanocatalyst

2015 ◽  
Vol 1086 ◽  
pp. 1-6 ◽  
Author(s):  
P. Yuvasree ◽  
K. Nithya ◽  
N. Neelakandeswari ◽  
N. Rajasekaran ◽  
K. Uthayarani ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Rhodamine B ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
Ag Nps ◽  
X Ray ◽  
Chemical Approach ◽  
Uv Visible ◽  
Photocatalytic Applications

Green chemical approach is adopted for the synthesis of silver nanoparticles (Ag NPs). Since the nanoparticles tend to aggregate during the course of the catalytic reaction, they need to be stabilized by employing a suitable template or a support. Hence the prepared nanoparticles were loaded on titania support and used as a photocatalyst. Silver loaded titania (Ag-TiO2) is characterized by UV-visible spectroscopy, powder X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic absorption spectroscopy (AAS). Prepared composite was also tested for its photocatalytic activity against the degradation of an industrial pollutant Rhodamine B dye. Effect of pH, ratio between the catalyst and the substrate and also the role of free radical generator in the degradation have been tested and summarized in this work.Keywords: Silver nanoparticles, Phytochemical preparation, Green chemical approach, Photocatalysis, Rhodamine B

Dicarbohydrazide based chemosensors for copper and cyanide ions via a displacement approach

2018 ◽  
Vol 42 (8) ◽  
pp. 6023-6033 ◽  
Author(s):  
Neetu Yadav ◽  
Ashok Kumar Singh
Keyword(s):  
Single Crystal ◽  
Elemental Analysis ◽  
Emission Spectra ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible ◽  
Displacement Approach ◽  
P Ligands

Ligands attached to pyridine dicarbohydrazide were synthesized and characterized using NMR, FT-IR, elemental analysis, UV-visible spectroscopy, mass spectrophotometry, emission spectra and single crystal X-ray diffraction, and were assessed for their ability to sense copper and cyanide ions.

scholarly journals Green synthesis of silk sericin-embedded silver nanoparticles and their antibacterial application against multidrug-resistant pathogens

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Md. Abdullah Al Masud ◽  
Hamid Shaikh ◽  
Md. Shamsul Alam ◽  
M. Minnatul Karim ◽  
M. Abdul Momin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Bombyx Mori ◽  
Multidrug Resistant ◽  
Visible Spectroscopy ◽  
Silk Sericin ◽  
X Ray ◽  
Synthesis Strategy ◽  
Uv Visible ◽  
Multidrug Resistant Pathogens

Abstract Background The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver nanoparticles (AgNPs) have been synthesized by natural products such as starch, soy protein, various extract of leaves, barks, and roots functioning both as reducing and stabilizing agents. Likewise, silk sericin (SS) is a globular protein discarded in the silk factory might be used for NP synthesis. In this research, we focus on the green synthesis and stabilization of AgNPs by SS as well as assessment of their antibacterial activities against some drug-resistant pathogen. Results SS was extracted from Bombyx mori silkworm cocoons in an aqueous medium. 17 w/w% of dry sericin powder with respect to the cocoon’s weight was obtained by freeze-drying. Furthermore, AgNPs conjugated to sericin, i.e., SS-capped silver nanoparticles (SS-AgNPs) were synthesized by easy, cost-effective, and environment-friendly methods. The synthesized SS-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction measurement. It has been found from the absorbance of UV-visible spectroscopy that a higher percent of SS-AgNPs was obtained at a higher concentration of silver nitrate solution. FTIR-ATR spectra showed that the carboxylate groups obtained from silk sericin act as a reducing agent for the synthesis of silver nanoparticles, while NH2+ and COO− act as a stabilizer of AgNPs. The X-ray diffractogram of SS-AgNPs was quite different from AgNO3 and sericin due to a change in the crystal structure. The diameter of AgNPs was around 20–70 nm observed using TEM. The synthesized SS-AgNPs exhibited strong antibacterial activity against multidrug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 20μg/mL. Conclusions This study encourages the use of Bombyx mori for the ecofriendly synthesis of SS-AgNPs to control multidrug-resistant microorganisms.

scholarly journals The Bio-fabrication of Gold Nanoparticles (AuNPs) by Green Method and Study of its Electrochemical Applications

2020 ◽  
Vol 1 (4) ◽  
pp. 22-25
Author(s):  
Azwan Morni
Keyword(s):  
Gold Nanoparticles ◽  
Ir Spectroscopy ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
Green Method ◽  
Oh Groups ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

This study reports a green method for the synthesis of gold nanoparticles (AuNPs) using the aqueous extract of Salix aegyptiaca extract. The effects of gold salt concentration, extract concentration and extract quantity were investigated on nanoparticles synthesis. Novel methods of ideally synthesizing AuNPs are thus thought that are formed at ambient temperatures, neutral pH, low costs and environmentally friendly fashion. AuNPs were characterized with different techniques such as UV–vis spectroscopy, FT-IR spectroscopy, X-ray diffraction, and TEM. FT-IR spectroscopy revealed that gold nanoparticles were functionalized with biomolecules that have primary carbonyl group, -OH groups and other stabilizing functional groups. TEM experiments showed that these nanoparticles are formed with various shapes and X-ray diffraction pattern showed high purity and face centered cubic structure of AuNPs. For electrochemical properties of AuNPs, a modified glassy carbon electrode using AuNPs (AuNPs/GCE) was investigated. The results show that electronic transmission rate between the modified electrode and [Fe (CN)6]3-/4- increased.

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