scholarly journals STATISTICAL OPTIMIZATION OF MEDIUM COMPONENTS BY PLACKETT BURMAN DESIGN AND RESPONSE SURFACE METHODOLOGY FOR ENHANCED ANTIOXIDANT ACTIVITY BY XYLARIA FEEJEENSIS HMJAU22039

2016 ◽  
Vol 8 (11) ◽  
pp. 159
Author(s):  
Durga Rebbapragada ◽  
Rajagopal Kalyanaraman
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Yeast Extract ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Statistical Optimization ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Burman Design ◽  
Plackett Burman Design

Objective: To enhance the antioxidant potential of Xylaria feejeensis by statistical optimization. The components of potato dextrose yeast extract (PDYEB) medium: potato infusion, dextrose and yeast extract were investigated to optimize antioxidant activity by Plackett-Burman design (P-B design). The interaction between the critical components of the medium and incubation time was further investigated by Response Surface Method (RSM) and the culture conditions were optimized for enhancing antioxidant activity. Central composite design (CCD) was employed and quadratic response surface model was used to analyze the data using analysis of variance (ANOVA).Methods: The antioxidant potential was measured by 1-1diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay, nitric oxide (NO) scavenging assay. The antioxidant activity was compared with total phenolic content measured by Folin-Ciocalteau (FC) reagent based assay.Results: Plackett-Burman design revealed dextrose and yeast extract to be the most significant components of PDYEB medium (p<.0001). ANOVA analysis showed that the model was highly significant (p<0.0001) for antioxidant activity measured by DPPH, NO scavenging and TPC (total phenolic content). The model was justified by applying the optimized conditions and values of 87.38%, 56.43%, 27.28 mg/g for DPPH, NO scavenging and TPC respectively were obtained. The estimated optimum conditions of the variables for the antioxidant activity and phenolic content are 12.72g of dextrose, 1.67g yeast extract, and incubation period of 23.43 d.Conclusion: The study highlights the importance of various components of the medium and the physiochemical limitations in antioxidant potential and phenol production of the fungal strain. 

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

Estimation of total Phenolic and ϑlavonoids content and Studies on antioxidant activity of different extracts of Olax scandens by FRAP assay

2020 ◽  
Vol 11 (3) ◽  
pp. 3367-3371
Author(s):  
Pranaya P ◽  
Akiladevi D
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Frap Assay ◽  
Aerial Parts ◽  
Reducing Ability ◽  
Ic50 Value

Olax scandens Roxb. (family Olacaceae) available in throughout tropical India. The current study, aerial parts of different concentrates of Olax scandens was evaluated for its in-vitro antioxidant potential by FRAP assay taking ascorbic acid as the standard and estimation of total phenolic content and flavonoids content. The IC50 value was originated that methanolic concentrates of Olax scandens are more efficient in antioxidant activity by FRAP methods compared EA & PE concentrates. The methanolic concentrates of Olax scandens & ascorbate exhibited antioxidant potential possessing IC50 207µg/ml & 50µg/ml by Ferric reducing ability Power assay. The methanolic and EA concentrates of Olax scandens showed the total phenolic content (14.426 ± 0.032, 4.128 ± 0.025) respectively, and ৒lavonoids content (11.526 ± 0.054, 3.682 ± 0.042) respectively. Invitro antioxidant studies show methanolic concentrates of Olax scandens have better antioxidant activity as well as a higher content of total phenolic and flavonoids content. These results indicate that aerial parts of methanolic concentrates Olax scandens could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

scholarly journals Optimization of the extraction of phenolic compounds and antioxidant activity from the roots of Waltheria ovata using the response surface methodology

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2095-2102
Author(s):  
O. Herrera-Calderon ◽  
R. Vega
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Sample Ratio

Waltheria ovata is a medicinal plant belonging to the Sterculiaceae genus. Natural products of Waltheria ovata could be used in the food industry as natural antioxidants due to its high content of polyphenols according to the literature. The main objective in this research was to optimize the extraction of phenolic compounds and the antioxidant activity from Waltheria ovata roots using response surface methodology (RSM). The total phenolic content in different extracts was determined by spectrophotometric method (Folin-Ciocalteu reagent) and the antioxidant activity by using DPPH assay. To optimize the conditions for total phenolic content and antioxidant activity were used three independent variables: solvent/sample ratio (1:10, 1:20 and 1:30 g/mL), temperature (40, 50, and 60°C) and time (40, 50 and 60 mins). The results showed that total phenolic content and antioxidant activity in the experiments ranged from 8.7 to 12.1 mg GAE/g and 76.1% to 96.7%, respectively. The coefficients of determination (R2 values) for phenolic content and antioxidant activity were 0.86 and 0.91, respectively. Under the optimum conditions of 1:20 g/mL, 60°C and 55 mins of extraction, the values for total phenolic content and antioxidant activity were 0.448±0.02 mg GAE/g and 87.00±2.0%, respectively. These data showed that the experimental responses were reasonably close to the predicted responses (0.444 mg GAE/g and 84.67%). Therefore, the results showed that Waltheria ovata can be used as antioxidant in foods.

Analysis of Antioxidant Activity and Total Phenolic Content in Garcinia mangostana Using Response Surface Methodology

2015 ◽  
Vol 1113 ◽  
pp. 217-222
Author(s):  
Ummi Kalthum Ibrahim ◽  
Amira Sofea Mahamad Husin ◽  
Ruzitah Mohd Salleh
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Garcinia Mangostana ◽  
Different Parts

This study investigates the antioxidant activity and total phenolic content on different parts of Garcinia mangostana which are pericarp, leaf, fruit, and seed. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to determine the antioxidant activity and the total phenolic content was determined by Folin-Ciocalteu method. Response surface method (RSM) was used to determine the effect of the two extraction variables: extraction time (30-120 minutes) and extraction temperature (30-50°C) on yield of total phenolic content and antioxidant activity of different parts of Garcinia mangostana. The significant factors on each experimental design response were identified from the analysis of variance (ANOVA). The best condition for total phenolic content of different parts of Garcinia mangostana was chosen based on the desirability obtained by using extraction temperature of 30°C and extraction time of 60.09 minutes, which resulted in 0.0274 mg GAE/g of total phenolic content in pericarp, 0.2501 mg GAE/g of total phenolic content in leaf, 0.0202 mg GAE/g of total phenolic content in fruit, and 0.0198 mg GAE/g of total phenolic content in seed of Garcinia mangostana. Under this conditions, it was found that the antioxidant activity of pericarp, leaf, fruit, and seed of Garcinia mangostana were 89.45%, 86.58%, 93.33%, and 78.80% of radical scavenging activity, respectively.

scholarly journals Optimization of Antioxidant Potential of Penicillium granulatum Bainier by Statistical Approaches

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Priyanka Chandra ◽  
Daljit Singh Arora
Keyword(s):  
Response Surface ◽  
Incubation Temperature ◽  
Classical Method ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Step Response ◽  
Total Phenolic ◽  
Burman Design ◽  
Statistical Approaches ◽  
Plackett Burman Design

A three-step optimization strategy which includes one-factor-at-a-time classical method and different statistical approaches (Plackett-Burman design and response surface methodology) that were applied to optimize the antioxidant potential of Penicillium granulatum. Antioxidant activity was assayed by different procedures and compared with total phenolic content. Primarily, different carbon and nitrogen sources were screened by classical methods, which revealed sucrose and NaNO3 to be the most suitable. In second step, Plackett-Burman design also supported sucrose and NaNO3 to be the most significant. In third step, response surface analysis showed 4.5% sucrose, 0.1% NaNO3, and incubation temperature of 25°C to be the optimal conditions. Under these conditions, the antioxidant potential assayed through different procedures was 78.2%, 70.1%, and 78.9% scavenging effect for DPPH radical, ferrous ion, and nitric oxide ion, respectively. The reducing power showed an absorbance of 1.6 with 68.5% activity for FRAP assay.

scholarly journals Effects of Water Deficit Irrigation on Phenolic Composition and Antioxidant Activity of Monastrell Grapes under Semiarid Conditions

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1301
Author(s):  
Eva P. Pérez-Álvarez ◽  
Diego S. Intrigliolo ◽  
María Pilar Almajano ◽  
Pilar Rubio-Bretón ◽  
Teresa Garde-Cerdán
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Deficit Irrigation ◽  
Phenolic Content ◽  
Negative Impact ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Vitis Vinifera L ◽  
Phenolic Composition

The high phenolic compound content of grapes makes them an important source of natural antioxidants, among other beneficial health properties. Vineyard irrigation might affect berry composition and quality. Regulated deficit irrigation (RDI) is a widely used strategy to reduce the possible negative impact of irrigation on grapes, improving grape composition and resulting in water savings. Monastrell grapevines (Vitis vinifera L.) grown in eastern Spain were subjected to two water regime strategies: rainfed (non-irrigation) and RDI. The content of anthocyanins, flavonols, flavanols, hydroxybenzoic and hydroxycinnamic acids, and stilbenes was determined by HPLC and was related with total phenolic content and three antioxidant activity methods (ABTS, DPPH, and ORAC). The study aimed to evaluate and compare the phenolic composition and antioxidant potential of Monastrell grapes. The rainfed regime concentrated grapes in terms of phenolic compounds. Thus, total content of anthocyanins, flavonols, flavanols, hydroxybenzoic acids, and total phenols were higher in the rainfed grapes than in the RDI ones. Besides, the rainfed grapes doubled their antioxidant potential with respect to the RDI grapes with the ORAC method. Total phenolic content and antioxidant activity by ORAC assay positively correlated with most of the total phenolic compounds analyzed. This study demonstrates how field practices can modulate final grape composition in relation to their antioxidant activity.

scholarly journals Spent Coffee Grounds’ Valorization towards the Recovery of Caffeine and Chlorogenic Acid: A Response Surface Methodology Approach

2021 ◽  
Vol 13 (16) ◽  
pp. 8818
Author(s):  
Georgia-Christina Mitraka ◽  
Konstantinos N. Kontogiannopoulos ◽  
Maria Batsioula ◽  
George F. Banias ◽  
Andreana N. Assimopoulou
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Chlorogenic Acid ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Value Added ◽  
Total Phenolic ◽  
Spent Coffee Grounds ◽  
Coffee Grounds

The amount of spent coffee grounds (SCGs) created, represents an environmental challenge worldwide. In this context, the aim of the present study was to exploit the potential of SCGs as a source of bioactive compounds that can be utilized in high value-added products. Thus, a cost-effective and environmentally friendly extraction technique was developed to ensure extracts with high total phenolic content and antioxidant activity, as well as significant amounts of caffeine and chlorogenic acid. Response surface methodology was implemented to evaluate the effects of the main extraction parameters (i.e., time, temperature, and ethanol-to-water ratio) and their interactions on the defined responses. The ethanol ratio was found to be the most significant variable. Then, a set of optimum values was determined (i.e., 7 min, 75 °C, and ethanol:water ratio 5:95), where the predicted values for responses were found to be 5.65% for the yield (Y1), 152.68 mg gallic acid equivalents per L for total phenolic content (Y2), 0.797 μmol Trolox equivalent per mL for the antioxidant activity (Y3), 30.5 ppm for caffeine concentration (Y4), and 17.4 ppm for chlorogenic acid concentration (Y5). Furthermore, the corresponding high experimental values from the validation experiment fitted well to these predictions, clearly clarifying the high potential of SCG extracts for use in high value-added applications.

scholarly journals IN VITRO EVALUATION OF ANTIOXIDANT POTENTIAL AND TOTAL PHENOLIC CONTENT OF BARLERIA LONGIFLORA LEAF EXTRACTS

2019 ◽  
pp. 212-215
Author(s):  
JOTHIMUNIYANDI M ◽  
JAYACHITRA A
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Metal Chelating ◽  
Reducing Ability

Objective: The aim of this present study is to estimate the antioxidant potential found in the leaves of Barleria longiflora that belongs to the family Acanthacea. Methods: Antioxidant activity of six different solvent extracts system of B. longiflora leaves was assayed for 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical activity, scavenging of hydrogen peroxide (H2O2), ferric reducing antioxidant activity, metal chelating ability assay, and total reducing ability. Total phenolic content of the extracts was determined by Folin–Ciocalteu method. Results: The radical scavenging activity was evaluated by DPPH of ethanol extract at concentration of 100 μg/mg was found to be 56.5% followed by methanol 48.4%, whereas maximum scavenging of H2O2 was observed in ethanol 83.4% followed by chloroform 70.8%. Ethanolic extract of B. longiflora leaves showed the highest value in ferric reducing antioxidant power assay 74.8%, metal chelating activity 61.6%, and total reducing ability 0.76±0.02 when compared to the standard ascorbic acid. Conclusion: The results suggest that the antioxidant potential of the ethanol extract has the highest activity in compared to other five extracts of B. longiflora leaves.

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