scholarly journals SYNTHESIS AND CHARACTERIZATION IN VITRO ANTIMICROBIAL AND CYTOTOXICITY TESTING OF OXALIC ACID-DERIVED CADMIUM CHELATING AGENTS

2018 ◽  
Vol 10 (7) ◽  
pp. 80
Author(s):  
Daisy Selasteen F ◽  
Alfred Cecil Raj S ◽  
Alagappa Moses A
Keyword(s):  
Antimicrobial Activity ◽  
Silica Gel ◽  
Optical Conductivity ◽  
Cancer Cell Line ◽  
Uv Spectra ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela

Objective: The aim of this study is to investigate the growth, structure, spectral, solubility and biological activity of sodium cadmium oxalate dehydrate (NaCdOx) and cadmium oxalate trihydrate (CdOx) crystals prepared by a single diffusion method in the silica gel medium.Methods: The present crystals were grown using single diffusion methods and tested for XRD, UV absorption (190 to 1100 mm) and solubility (distilled water at 20-29 °C) studies. The antimicrobial efficacy of the grown samples at various concentrations (25, 50, 75 and 100 μg/ml) was studied against Streptococcus, (G+Ve), Pseudomonas aeruginosa (G-Ve) and Candida albicans (antifungal). The cytotoxicity evolution was carried out against human cervical cancer cell line (HeLa) using MTT assays.Results: The existing single crystals were successfully grown by silica gel technique. The solubility of sodium cadmium oxalate dehydrate (NaCdOx) was moderately good in deionized warm water. The FTIR spectral studies confirmed the chelating bands of the present samples and UV spectra showed the better the optical conductivity of as-grown crystals. The complexes showed good antimicrobial activity against all tested microbial strains and they exhibited a decrease in cytotoxicity activity.Conclusion: The gel method was suitable to grow metal complexes of legend crystals. The modification of structural properties of cadmium oxalate trihydrate (CdOx) by sodium doping was much improved the solubility, anticancer, antimicrobial activity and polarization by the high optical conductivity of sodium cadmium oxalate dehydrate (NaCdOx) compound. Hence sodium cadmium oxalate dehydrate (NaCdOx) might be a candidate for biomedical applications. 

Synthesis, Characterization and Biological Studies of a Organoselenium trans-Palladium(II) Complexes

2020 ◽  
Vol 16 ◽  
Author(s):  
Ivana Raković ◽  
Jovana Bogojeski ◽  
Katarina Mladenović ◽  
Angelina Petrović ◽  
Vera Divac ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell Line ◽  
Spectral Studies ◽  
Cytotoxic Effects ◽  
Hct 116

Background: Over the years, transition metal complexes have exhibited significant antimicrobial and antitumor activity. It all started with cisplatin discovery, but due to the large number of side effects it shows, there is a growing need to find a new metal-based compound with higher selectivity and activity on more tumors. Objectives: Two novel trans-palladium(II) complexes with organoselenium compounds as ligands, [Pd(L1)2Cl2] (L1 = 5- (phenylselanylmethyl)-dihydrofuran-2(3H)-one) and [Pd(L2)2Cl2] (L2 = 2-methyl-5-(phenylselanylmethyl)- tetrahydrofuran) were synthesized, in the text referred to as Pd-Se1 and Pd-Se2. Also, a structurally similar trans-palladium(II) complex, [Pd(L3)2Cl2] (L3= 2,2-dimethyl-3-(phenylselanylmethyl)-tetrahydro-2H-pyran ) was synthesized according to an already published work and is referred to as Pd-Se3. The interaction of synthesized complexes with DNA and bovine serum albumin were done. Also, antimicrobial activity and in vitro testing, cell viability, and cytotoxic effects of synthesized ligands and complexes on human epithelial colorectal cancer cell line HCT-116 were studied. Molecular docking simulations were performed to understand better the binding modes of the complexes reported in this paper with DNA and BSA, as well as to comprehend their antimicrobial activity. Methods: The interactions of the synthesized complexes with DNA and bovine serum albumin were done using UV-Vis and emission spectral studies as well as docking studies. Antimicrobial activity was tested by determining the minimum inhibitory concentrations (MIC) and minimum microbicidal concentration (MMC) using the resazurin microdilution plate method. Cytotoxic activity on cancer cells was studied by MTT test. Results: The Pd(II) complexes showed a significant binding affinity for calf thymus DNA and bovine serum albumin by UV-Vis and emission spectral studies. The intensity of antimicrobial activity varied with the complexes Pd-Se1 and Pd-Se3, showing significantly higher activity than the corresponding ligand. The most significant activity was shown on Pseudomo-nas aeruginosa. Under standardized laboratory conditions for in vitro testing, cell viability and cytotoxic effects of synthesized ligands and complexes were studied on human epithelial colorectal cancer cell line HCT-116, where Pd-Se2 showed some significant cytotoxic effects. Conclusion: The newly synthesized complexes have the potential to be further investigated as metallodrugs.

In vitro Study on the Effect of Nanoparticles and Transfersomes as Targeted Drug Carrier for Cancer

2019 ◽  
Vol 9 (4) ◽  
pp. 512-518
Author(s):  
Karthikeyan Muthu ◽  
Blessy Vijayakumar ◽  
Thirumurugan Alagu
Keyword(s):  
Gold Nanoparticles ◽  
Drug Carrier ◽  
Cancer Cell Line ◽  
Sem Analysis ◽  
Cervical Cancer Cell Line ◽  
Hela Cell Lines ◽  
Cell Line Hela ◽  
Uv Visible ◽  
Vitro Effect

Introduction: Cancer is one of the current leading cause of death all over the world. Among the various emerging technologies, nanotechnology plays a prominent role in delivering the drug to the target region. Materials and Methods: In this study, the In vitro effect of doxorubicin adsorbed gold nanoparticles synthesized by Azadirachta Indica leaves extract as reducing agent and the doxorubicin entrapped modified liposomes called transfersomes was compared over the cervical cancer cell line (HeLa cell lines). The synthesized gold nanoparticles were characterized using a UV-visible spectrophotometer, SEM analysis. Results: The UV-Visible spectrum showed the peak at 537nm and the incorporation of drug over the nanoparticles was conformed using FTIR and SEM analysis. The drug entrapment onto transfersomes was also characterized using FTIR and SEM analysis. When compared, the drug entrapped transfersomes shows significant effect with the lowest concentration of drug (0.25 µg/mL) than the drug adsorbed nanoparticles. Conclusion: Hence, the transfersomes may also become the promising drug carrier in the future.

In vitro and in vivo anti-cancer activity of formononetin on human cervical cancer cell line HeLa

Tumor Biology ◽  
2013 ◽  
Vol 35 (3) ◽  
pp. 2279-2284 ◽  
Author(s):  
Yue-mei Jin ◽  
Tian-min Xu ◽  
Yan-hui Zhao ◽  
Yi-chao Wang ◽  
Man-hua Cui
Keyword(s):  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela ◽  
Anti Cancer ◽  
Human Cervical Cancer Cell ◽  
Line Hela ◽  
Cancer Activity ◽  
Human Cervical Cancer

scholarly journals Synthesis of 3-(2-(subsituted-(trifluoromethyl)phenylamino)acetyl)-2H-chromen-2-one derivatives as new anticancer agents

2022 ◽  
Vol 11 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Anees Pangal ◽  
Yusufi Mujahid ◽  
Bajarang Desai ◽  
Javed A. Shaikh ◽  
Khursheed Ahmed
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Solvent Free Conditions ◽  
Cell Line Hela ◽  
Mcf 7

Under solvent free conditions and in presence of a base 3-(2-(subsituted-(trifluoromethyl)phenylamino)acetyl)-2H-chromen-2-one derivatives were synthesized by grinding technique. Structural investigations were carried out with IR studies, HRMS, 1HNMR and 13CNMR. The compounds were checked for their in vitro anticancer activities against three different human cancer cell lines viz human breast cancer cell line (MCF-7), human cervical cancer cell line (HeLa) and human oral squamous cell carcinoma (SCC-40) using SRB method. All the title compounds showed low toxicity towards non-malignant PBMC cells indicating their tumour selectivity. The compounds exhibited good in vitro anti-proliferative potency at lower concentrations against HeLa and MCF-7 cell lines and remain moderately active against SCC-40.

scholarly journals Surfactant-Ruthenium(II) Complexes: Synthesis, Characterization, DNA Binding, Anticancer and Antimicrobial Activity

2019 ◽  
Vol 31 (9) ◽  
pp. 1931-1942
Author(s):  
R. Suganthi Devi ◽  
N. Kumaraguru
Keyword(s):  
Antimicrobial Activity ◽  
Cancer Cell Line ◽  
Antimicrobial Activities ◽  
Spectroscopic Technique ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela ◽  
Human Cervical Cancer Cell ◽  
The Impact ◽  
Ct Dna ◽  
Line Hela

The interaction of ligand bound ruthenium(II) complexes with DNA have grown fame because of their relevance in the development of new reagents for medicinal applications and the impact of dominating cisplatin. Surfactant-ruthenium(II) complexes [Ru(DMP)2(DA)Cl](ClO4) (1) and [Ru(DMP)2(DA)2](ClO4)2 (2) with primary ligand as DMP (2,9-dimethyl[1,10]-phenanthroline) and secondary ligand as dodecyl amine (DA) were synthesized and characterized. The critical micelle concentration (CMC) of complexes in aqueous solution were obtained from conductivity measurements. The interaction of surfactant-ruthenium(II) complexes with CT-DNA has been explored by spectroscopic technique and viscosity dimensions. These complexes were tested for cytotoxic and antimicrobial activities with human cervical cancer cell line (HeLa) and pathogenic microorganisms. The results indicate that the complex 2 binds more strongly to DNA than complex 1 further affecting the viability of the cells significantly and also showed moderate antimicrobial activity.

scholarly journals Synthesis, thermal and antitumour studies of Th(IV) complexes with furan-2-aldehyde-N-phenyl thiosemicarbazone

2010 ◽  
Vol 75 (6) ◽  
pp. 749-761 ◽  
Author(s):  
G. Rajendran ◽  
C.S. Amritha ◽  
Ruby Anto ◽  
Vino Cheriyan
Keyword(s):  
Schiff Base ◽  
Antitumour Activity ◽  
Cancer Cell Line ◽  
Bidentate Ligand ◽  
Cervical Cancer Cell Line ◽  
Kinetic And Thermodynamic Parameters ◽  
Composition And Structure ◽  
Cell Line Hela ◽  
Human Cervical Cancer Cell

Thorium(IV) complexes with the Schiff base furan-2-aldehyde-N-phenyl thiosemicarbazone (L) were synthesised and characterized. The composition and structure of the metal complexes were proposed base on elemental analysis, molar conductivity measurements, FTIR and 1H-NMR spectroscopy. The Schiff base behaves as a neutral bidentate ligand coordinating through the azomethine N and the thioketo S atoms. From various studies, complexes were ascertained the general formula [ThL2X4] and [ThL2Y2] where X represents the monovalent anions NO3-, NCS-, CH3COO-, CH3CHOHCOO-, ClO4-,and Y the bivalent anions SO4 2-and C2O4 2-. The thermal behaviour of the nitrato and oxalato complexes was studied and kinetic and thermodynamic parameters were calculated using the Coats-Redfern Equation. The ligand and a representative complex [ThL2(NO3)4] were screened in vitro for their antitumour activity against the human cervical cancer cell line (Hela).

scholarly journals Synthesis, Spectral Analysis, Molecular Docking and Biological Evaluation of Cyclohepta[b]indole Derivatives

2019 ◽  
Vol 92 (3) ◽  
pp. 347-356 ◽  
Author(s):  
Ayyachamy Pandian Amuthavalli ◽  
Babu Prakash ◽  
David Edison ◽  
Rajendran Velmurugan
Keyword(s):  
Cancer Cell Line ◽  
Docking Studies ◽  
Biological Evaluation ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Cervical Cancer Cell Line ◽  
Indole Derivatives ◽  
Standard Drug ◽  
Cell Line Hela

A new series of specifically substituted cyclohepta[b]indole derivatives from the precursor thiophen-2-ylmethylene has been synthesized. The structures of synthesized derivatives were established by spectral and elemental analyses. The docking studies with protein kinase CK2 was performed, derivative 6c exhibited the most excellent glide and E model score of –7.61 and –58.27, respectively. In-vitro anticancer activity against cervical cancer cell line (HeLa) was studied. The IC50 values were compared with the standard drug Ellipticine. Compounds 5c, 6c and 6d showed better IC50 value when compared to the other molecules. The derivatives were evaluated for their antibacterial activity against the reference drugs Sparfloxacin and Norfloxacin using agar dilution method. The derivatives 4a–d exhibited better MIC values against Gram-positive bacteria and Gram-negative bacteria when compared with remaining derivatives. Structure activity relationship (SAR) analyses established that the derivatives are potential lead compounds for future drug development studies.

Chromene–triazole-pyrimidine based chemosensor therapeutics for the in vivo and in vitro detection of Fe3+ ions

2021 ◽  
Vol 45 (15) ◽  
pp. 6760-6767
Author(s):  
Aranhikkal Shamsiya ◽  
Damodaran Bahulayan
Keyword(s):  
Cervical Cancer ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela ◽  
Human Cervical Cancer Cell ◽  
Line Hela ◽  
Click Synthesis ◽  
Human Cervical Cancer

MCR and click synthesis of selective and sensitive Fe3+ sensors that can interact with CDK2 proteins and cytotoxicity against the human cervical cancer cell line HeLa is developed.

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