Radiosensitizing effects of citrate-coated cobalt and nickel ferrite nanoparticles on breast cancer cells

Aim: Evaluation of the biocompatibility and radiosensitizer potential of citrate-coated cobalt (cit-CF) and nickel (cit-NF) ferrite nanoparticles (NPs). Materials & methods: Normal fibroblast and breast cancer cells were treated with different concentrations of citrate-coated ferrite NPs (cit-NPs) and irradiated with a cobalt-60 source at doses of 1 and 3 Gy. After 24 h, cell metabolism, morphology alterations and nanoparticle uptake were evaluated. Results: Cit-CF and cit-NF NPs showed no toxicity to normal cells up to 250 and 100 μg.ml-1, respectively. Combination of cit-NP and ionizing radiation resulted in up to fivefold increase in the radiation therapeutic efficacy against breast cancer cells. Conclusion: Cit-CF and cit-NF NPs are suitable candidates for application as breast cancer cell radiosensitizers.

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Effect of Exogenous pH on Cell Growth of Breast Cancer Cells

10.21203/rs.3.rs-260226/v1 ◽

2021 ◽

Author(s):

Sungmun Lee

Keyword(s):

Breast Cancer ◽

Cell Growth ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Cell Metabolism ◽

Acidic Ph ◽

Normal Cells ◽

M Phase ◽

Life Threatening ◽

Acidic Conditions

Abstract Breast cancer is the most common cancer in women and the most life-threatening cancer in women worldwide. One key feature of cancer cells including breast cancer cells is a reversed pH gradient, and extracellular pH (pHe) of cancer cells is more acidic than normal cells. Cancer cells have lower pHe of ~ 6.7–7.1 and higher intracellular pH (pHi) of 7.4, while normal cells have pHe of 7.4 and lower pHi of 7.2. Here, we investigated how exogenous pH affected breast cancer cells. MDA-MB-231 cell lines were cultured in five different pHs, pH 6.0, pH 6.7, pH 7.4, pH 8.4, and pH 9.2 of medium. The cells were growing in pH 6.0 and pH 9.2 however, not as fast as in other pHs. Especially they were floating in more acidic conditions than pH 6.3. In alkaline pH (pH 8.4 and pH 9.2), more cells were early apoptotic and they were in S phase. In acidic pH (pH 6.0), more cells were late apoptotic or necrotic and more cells were at G2/M phase in acidic pH (pH 6.0 and pH 6.7). The results suggested that MDA-MB-231 cells experienced different cell growth and cell metabolism in different pHs.

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Preferential Dependence of Breast Cancer Cells versus Normal Cells on Integrin-Linked Kinase for Protein Kinase B/Akt Activation and Cell Survival

Cancer Research ◽

10.1158/0008-5472.can-05-2304 ◽

2006 ◽

Vol 66 (1) ◽

pp. 393-403 ◽

Cited By ~ 85

Author(s):

Armelle A. Troussard ◽

Paul C. McDonald ◽

Elizabeth D. Wederell ◽

Nasrin M. Mawji ◽

Nolan R. Filipenko ◽

...

Keyword(s):

Breast Cancer ◽

Protein Kinase ◽

Cell Survival ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Protein Kinase B ◽

Normal Cells ◽

Akt Activation ◽

Integrin Linked Kinase

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Size and Chemistry Controlled Cobalt-Ferrite Nanoparticles and Their Anti-proliferative Effect against the MCF-7 Breast Cancer Cells

ACS Biomaterials Science & Engineering ◽

10.1021/acsbiomaterials.6b00333 ◽

2016 ◽

Vol 2 (12) ◽

pp. 2139-2152 ◽

Cited By ~ 23

Author(s):

Sumayya M. Ansari ◽

Renuka D. Bhor ◽

Kalpana R. Pai ◽

Subhasish Mazumder ◽

Debasis Sen ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Cobalt Ferrite ◽

Ferrite Nanoparticles ◽

Cobalt Ferrite Nanoparticles ◽

Proliferative Effect ◽

Mcf 7

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The in vitro therapeutic activity of betulinic acid nanocomposite on breast cancer cells (MCF-7) and normal fibroblast cell (3T3)

Journal of Materials Science ◽

10.1007/s10853-014-8526-3 ◽

2014 ◽

Vol 49 (23) ◽

pp. 8171-8182 ◽

Cited By ~ 15

Author(s):

Samer Hasan Hussein-Al-Ali ◽

Palanisamy Arulselvan ◽

Sharida Fakurazi ◽

Mohd Zobir Hussein

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Betulinic Acid ◽

Breast Cancer Cells ◽

Fibroblast Cell ◽

Normal Fibroblast ◽

Therapeutic Activity ◽

Mcf 7

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A preliminary study: The selective effect of bee venom on inhibition of cell migration in metastatic breast cancer cells over normal cells in comparison with cisplatin

Journal of Apitherapy and Nature ◽

10.35206/jan.835042 ◽

2021 ◽

Author(s):

Soner KARABULUT ◽

Selcen ÇELİK UZUNER

Keyword(s):

Breast Cancer ◽

Cell Migration ◽

Metastatic Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Metastatic Breast ◽

Bee Venom ◽

Normal Cells ◽

Selective Effect ◽

Preliminary Study

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Research Article Combination of endostatin and vinorelbine enhances control of breast cancer cells while endostatin ameliorates the toxicity of vinorelbine in normal cells

Genetics and Molecular Research ◽

10.4238/gmr18497 ◽

2020 ◽

Vol 19 (1) ◽

Author(s):

Cansu Kilit ◽

E.A. Aydemir ◽

K. Fiskin

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Normal Cells ◽

Research Article

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Stem cell-like breast cancer cells with acquired resistance to metformin are sensitive to inhibitors of NADH-dependent CtBP dimerization

Carcinogenesis ◽

10.1093/carcin/bgy174 ◽

2019 ◽

Vol 40 (7) ◽

pp. 871-882 ◽

Cited By ~ 12

Author(s):

Arindam Banerjee ◽

Charles N Birts ◽

Matthew Darley ◽

Rachel Parker ◽

Alex H Mirnezami ◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Stem Cell ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Cyclic Peptide ◽

Cell Metabolism ◽

Acquired Resistance ◽

Therapy Resistance ◽

Cell Gene Expression

AbstractAltered flux through major metabolic pathways is a hallmark of cancer cells and provides opportunities for therapy. Stem cell-like cancer (SCLC) cells can cause metastasis and therapy resistance. They possess metabolic plasticity, theoretically enabling resistance to therapies targeting a specific metabolic state. The C-terminal binding protein (CtBP) transcriptional regulators are potential therapeutic targets in highly glycolytic cancer cells, as they are activated by the glycolytic coenzyme nicotinamide adenine dinucleotide (NADH). However, SCLC cells commonly exist in an oxidative state with low rates of glycolysis. Metformin inhibits complex I of the mitochondrial electron transport chain; it can kill oxidative SCLC cells and has anti-cancer activity in patients. SCLC cells can acquire resistance to metformin through increased glycolysis. Given the potential for long-term metformin therapy, we have studied acquired metformin resistance in cells from the claudin-low subtype of breast cancer. Cells cultured for 8 weeks in sub-IC50 metformin concentration proliferated comparably to untreated cells and exhibited higher rates of glucose uptake. SCLC cells were enriched in metformin-adapted cultures. These SCLC cells acquired sensitivity to multiple methods of inhibition of CtBP function, including a cyclic peptide inhibitor of NADH-induced CtBP dimerization. Single-cell mRNA sequencing identified a reprogramming of epithelial–mesenchymal and stem cell gene expression in the metformin-adapted SCLC cells. These SCLC cells demonstrated an acquired dependency on one of these genes, Tenascin C. Thus, in addition to acquisition of sensitivity to glycolysis-targeting therapeutic strategies, the reprograming of gene expression in the metformin-adapted SCLC cells renders them sensitive to potential therapeutic approaches not directly linked to cell metabolism.

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