scholarly journals Screening of Kunun-zaki for Methicillin-Resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Staphylococcus aureus (VRSA) and Extended-Spectrum Beta-Lactamase (ESBL) Producing Salmonella spp.

2020 ◽  
Vol 6 (11) ◽  
pp. 4013-4024
Author(s):  
Edward Kelechi ◽  
◽  
Daniel Emeaso ◽  
Alozie Chukwuemeka ◽  
Owuamalam Precious ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Foodborne Pathogens ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Beta Lactamase ◽  
Salmonella Spp ◽  
Methicillin Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Vancomycin Resistant

Kunun-zaki is an indigenous, fermented, non-alcoholic cereal-based beverage produced and consumed primarily in Northern Nigeria. Due to its high nutritional and moisture content, it provides an ideal environment for the growth of foodborne pathogens. This study determined the presence of Methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant Staphylococcus aureus (VRSA) and Extended spectrum beta lactamase (ESBL) producing Salmonella spp in Kunun-zaki sold in Umuahia metropolis, Nigeria. Out of the 40 Kunun-zaki samples examined, Salmonella spp was detected in 19 (47.5%), whereas S. aureus was detected in 22 (55%) of the samples. The total Salmonella count ranged from 1.90×105-9.70×106 (CFU/ml), whereas the total S. aureus count ranged from 3.40×104-7.50×106 (CFU/ml); these microbial counts do not conform to the standard limits of detectable microbes in ready-to-eat food samples as stipulated by the Center for Food Safety. All the isolates were subjected to antibiotic susceptibility testing using the modified Kirby Bauer disc diffusion method. On the one hand, most of the Salmonella spp were resistant to the beta-lactam class of antibiotics used (IMP 58%, MEM 90%, AMP 53%). On the other hand, the Salmonella isolates showed the highest sensitivities to ceftazidime (95%) and ofloxacin (90%). The S. aureus isolates revealed high susceptibility to ofloxacin (96%), and major resistance to oxacillin (41%) and the oxyimino-cephalosporin antibiotics used (CAZ 55%, CTR 41%). MRSA, VRSA and ESBL producing Enterobacteriaceae are amongst the most critical multidrug-resistant bacterial pathogens. In this study, 26% of the Salmonella spp isolates were confirmed to be ESBL producers, whereas 41% of the isolates were MRSA, 23% were VRSA, and 68% were Vancomycin intermediate Staphylococcus aureus (VISA). Hence, the Kunun-zaki could constitute a reservoir for the dissemination of multidrug-resistant foodborne pathogens if not correctly quality controlled.

scholarly journals Detection of Antimicrobial Activity of Cranberry Fruit Extract against Methicillin Resistant Staphylococcus aureus and Extended Spectrum Beta Lactamase Escherichia coli

2021 ◽  
pp. 44-52
Author(s):  
Ali Nawaz Bijarani ◽  
Muhammad Owais Ismail ◽  
Zahida Memon ◽  
Faisal Afridi ◽  
Shabana Qabulio ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bacterial Activity ◽  
Beta Lactamase ◽  
Fruit Extract ◽  
Methicillin Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Fruit Extracts ◽  
Extended Spectrum

Objective: Antibacterial effects of Cranberry fruit extract on Methicillin Resistant Staphylococcus aureus and Extended Spectrum beta lactamase producing E. coli and its comparison with Linezolid and Meropenem. Materials and Methods: It is a Pre Clinical (in-vitro) study conducted in Ziauddin University from January 2020 to October 2020. All samples were collected from Ziauddin University.  All clinical samples were collected inform of pus, urine, blood, tracheal aspirations, patients admitted in surgical and medical wards, intensive care units and outdoor patients who were attending clinics. All these samples were transported to Clinical Microbiology Laboratory, Ziauddin hospital, North Nazimabad campus and then culture and sensitivity test were performed there. Sample showing double growth and contamination on agar plates were excluded from study. Results: Out of 80 samples included in this study 46(57.5%) were female and 34(42.5%) samples were male; female to male samples ratio of 1.35:1.The mean age was 45.71±11.83 years. MRSA commonly found in pus swab 15(37.5%) and 21(52.5%) ESBL producing E. coli found in urine samples. 14(35%) samples were observed anti-bacterial activity of cranberry fruit extracts against Extended Spectrum Beta Lactamase Producing Escherichia coli at 50 mg/ml concentration, followed by 10(25%) and 9(22.5%) samples at  60 mg/ml, 40 mg/ml were respectively. While resistance of Extended Spectrum Beta Lactamase Producing Escherichia coliwere observed high at different concentration level of cranberry fruit extracts. Good anti-bacterial activity of cranberry fruit extracts observed  against Methicillin Resistant Staphylococcus aureus at different                      levels of concentration 20(50%), 23(57.5%), 21(52.5%), 26(65%), 29(72.5%) samples were 20 mg/ml,30mg/ml,40mg/ml, 50mg/ml, 60mg/ml respectively. Most superior and best dose of cranberry fruit extract against Staph Aureus in about 72.5% (29) at 60mg/ml and their Comparison with linezolid and meropenem against Methicillin Resistant Staphylococcus aureus and found best positive results as compared with Linezolid and found significant p value 0.005. Conclusions: Cranberry extract has a lot of potential to prove itself to be a good antimicrobial agent. The cranberry fruit extract has high antimicrobial activity against methicillin resistant S.aureus and resistant strains of E. coli in comparison to linezolid and meropenem.

scholarly journals Identifikasi Isolat Klinik Methicillin-resistant Staphylococcus aureus dan Extended-Spectrum Beta-Lactamase Menggunakan Teknik Polimerase Chain Reaction

2020 ◽  
Vol 9 (1) ◽  
pp. 67
Author(s):  
Linosefa Linosefa
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Beta Lactamase ◽  
Chain Reaction ◽  
Methicillin Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

Bakteri patogen yang resisten terhadap banyak obat  seperti Staphylococcus aureus yang kebal terhadap Methicillin (Methicillin-resistant Staphylococcus aureus/MRSA) telah menjadi agen penyebab infeksi baik di lingkungan pelayanan kesehatan maupun di masyarakat. Extended-Spectrum Beta-Lactamase (ESBL) yang berasal dari mutasi β-laktamase juga menjadi masalah utama dalam infeksi terkait pelayanan kesehatan. Tujuan: Menentukan proporsi bakteri penghasil MRSA dan ESBL dari isolat klinis di Rumah Sakit Dr. M. Djamil Padang menggunakan teknik Polimerase Chain Reaction (PCR). Metode: Penelitian ini merupakan studi prospektif dengan mengumpulkan isolat Staphylococcus aureus dan Enterobacteriaceae dari Juli hingga September 2017. Penelitian dilakukan di Fakultas Kedokteran Universitas Andalas untuk mengkonfirmasi bakteri penghasil MRSA dan ESBL. Hasil: Sebanyak 15 dari 27 isolat Staphylococcus aureus (56%) merupakan  MRSA dan 21 dari 41 isolat Enterobacteriaceae (51%) adalah penghasil ESBL. Klebsiella sp. paling banyak ditemukan di antara isolat Enterobacteriaceae (59%). Lima puluh persen Klebsiella sp menghasilkan  ESBL. Simpulan: Studi ini menunjukkan bahwa setengah isolat klinis dari RSUP Dr. M. Djamil merupakan MRSA dan penghasil ESBL. Deteksi dini MRSA dan Enterobacteriaceae penghasil ESBL perlu dilakukan, untuk mencegah dan mengurangi transmisi bakteri MDR di lingkungan rumah sakit.Kata kunci: Enterobacteriaceae, Klebsiella sp, MDR, Staphylococcus aureus

scholarly journals Inhibitory activity of fermentation filtrate of red passion fruit pulp (Passiflora edulis sims.) against Escherichia coli extended-spectrum beta-lactamase (ESBL) and methicillin resistant Staphylococcus aureus (MRSA)

2020 ◽  
Vol 26 (1) ◽  
pp. 22-25
Author(s):  
Iif Hanifa Nurrosyidah ◽  
Ni Made Mertaniasih ◽  
Isnaeni Isnaeni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Passion Fruit ◽  
Beta Lactamase ◽  
Fruit Pulp ◽  
Passiflora Edulis ◽  
Methicillin Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

This study aims to determine the minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) of the fermentation filtrate (FF) on red passion fruit pulp (Passiflora edulis Sims.) against Escherichia coli Extended Spectrum Beta Lactamase (ESBL) and Methicillin Resistant Staphylococcus aureus (MRSA). The method used was the fermentation of red passion fruit pulp for 24 h using De Man Rogosa Sharpe Broth (MRS-broth) media, and the result showed that the FF of red passion fruit pulp made a dilution series concentration of 50 percent, 25 percent, 12.5 percent, 6.25 percent. Considering this result, the MIC FF of red passion fruit cells against ESBL and MRSA has a 25 percent dilution concentration, while MBC has a 50 percent dilution concentration. This shows that red passion fruit has the potential to be developed as antibacterial material, especially to fight bacteria that are already resistant.

Sign in / Sign up

Export Citation Format

Share Document