A Comparative Study of Erythrocyte Sedimentation Rate using Saline Diluted and Undiluted EDTA with TSC

Erythrocyte sedimentation rate (ESR) is the rate at which RBC sediment in a period of 1 hr. It is a common Haematology test that is a non-specific measure of inflammation and it became a common screening test worldwide for acute phase proteins and chronic diseases. The International Council for Standardization in Haematology (ICSH) recommended the Westergren method as the method of choice for ESR determination. TSC is almost exclusively used as the diluent of choice for setting up ESR, but some contemporary laboratories have resolved to use Normal saline (NS) as the diluent of choice while other set ESR using EDTA anticoagulated Blood (BLD) without any diluent. The objective of this study is to assess the comparison between saline diluted and undiluted EDTA with TSC as an anticoagulant in ESR detection and to find out any gender wise variations by using these anticoagulants. A total of 50 students were participated in this study. From each of the participants 5 ml of BLD was collected and it is then divided into 3 parts. 1.6 ml BLD to 0.4ml 3.8% TSC tube, 1.6 ml EDTA blood to 0.4 ml NS tube. 3rd tube with 2 ml EDTA BLD and set for ESR and obtained result within 1 hour. The Result is the mean +SD value of ESR were 19.48+5.7 mm/hr. in undiluted EDTA, 15.22+4.6 mm/hr. in saline diluted EDTA & 15.36+4.5 mm/hr. in TSC. The mean difference of ESR value between saline diluted EDTA with TSC BLD was 0 and it with undiluted EDTA was 4 mm /hr. The study indicates that there was a significant difference between ESR value with undiluted EDTA and TSC while diluted EDTA and TSC were there is no significant difference. The mean +SD of ESR value using undiluted, diluted EDTA and TSC in males were 16.20+3.3, 11.05+2.8, 11.29+2.6 and while it for females were 21.69+5.2, 17.36+3.8, 17.45+3.8 respectively. In conclusion, TSC is the best diluent to be used in contemporary lab to set ESR as compared to EDTA BLD. But we can use saline diluted EDTA as an alternative to citrate diluted BLD to set ESR and it also showed there is a gender wise variation in ESR using these anticoagulants.

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A comparison of erythrocyte sedimentation rates of bloods anticoagulated with trisodium citrate and EDTA among TB presumptive patients at the University of Gondar comprehensive specialized hospital, northwest Ethiopia

10.21203/rs.2.16918/v3 ◽

2020 ◽

Author(s):

Zegeye Getaneh ◽

Fekadu Ayelgn ◽

Geletaw Asemahegn ◽

Habtamu Geleta ◽

Aregawi Yalew ◽

...

Keyword(s):

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Sodium Citrate ◽

Trisodium Citrate ◽

Northwest Ethiopia ◽

Cross Sectional ◽

Erythrocyte Sedimentation ◽

Significant Difference ◽

The Mean ◽

The University

Abstract Objective: The purpose of this study was comparing the erythrocyte sedimentation rate (ESR) results of trisodium citrate (TSC) and ethylene diamine tetra-acetic acid (EDTA) anticoagulants. A comparative cross-sectional study was conducted at the University of Gondar specialized referral hospital, northwest, Ethiopia. A total of 70 TB presumptive participants were recruited. From each of the 70 participants of the study, 3 and 1.6 ml of blood was collected in EDTA tubes and 0.4 ml of trisodium Citrate anticoagulant containing test tubes, respectively. Results: The mean ± SD values of ESR were 57.9 ± 41.45 mm/hr in EDTA and 50.99 ± 43.5 mm/hr in TSC anticoagulated blood. The mean difference of ESR values between EDTA and TSC blood (6.91 ± 13.66 mm/hr) was statistically significant. The Mean ± SD of ESR values using EDTA and TSC in males were 59.57 ± 42.31 and 53.57 ± 44.61 mm/hr while for females it was 54.71±40.44 and 46.04 ± 41.82 mm/hr, respectively. The study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants. Keywords: Erythrocyte sedimentation rate, EDTA, Tri-sodium citrate, Westergren Method, Comparison

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Comparison of erythrocyte sedimentation rate between blood anticoagulated with sodium citrate and EDTA among Tuberculosis suspected patients at University of Gondar Hospital, North West Ethiopia

10.21203/rs.2.16918/v2 ◽

2019 ◽

Author(s):

Zegeye Getaneh ◽

Fekadu Ayelgn ◽

Geletaw Asemahegn ◽

Habtamu Geleta ◽

Aregawi Yalew ◽

...

Keyword(s):

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Sodium Citrate ◽

Northwest Ethiopia ◽

Cross Sectional ◽

North West ◽

Erythrocyte Sedimentation ◽

Significant Difference ◽

The Mean ◽

Study Participants

Abstract Objective: The purpose of this study was comparison of erythrocyte sedimentation rate results of sodium citrate and ethylene diamine tetra-acetic acid anticoagulant. A comparative cross-sectional study was conducted at University of Gondar Referral Hospital, Northwest, Ethiopia. A total of 70 TB suspected study participants were recruited. From each study participants, 3ml blood was collected in to EDTA tube and 1.6ml blood was collected in to a test tube containing 0.4ml Sodium Citrate anticoagulant. Results: The mean ± SD values for ESR were 57.9 ± 41.45 in EDTA and 50.99 ± 43.5 in citrate anticoagulated blood. The mean difference of ESR values between EDTA and citrated blood was 6.91 ± 13.66 mm/hr with a significant difference. The mean ± SD of ESR for EDTA and citrate blood was 59.57 ± 42.31 and 54.71 ± 40.44 mm/hr for males and 53.57 ± 44.61 and 46.04 ± 41.82 mm/hr for females, respectively. This study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants.

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A comparison of erythrocyte sedimentation rates of bloods anticoagulated with trisodium citrate and EDTA among TB presumptive patients at the University of Gondar comprehensive specialized hospital, northwest Ethiopia

10.21203/rs.2.16918/v4 ◽

2020 ◽

Author(s):

Zegeye Getaneh ◽

Fekadu Ayelgn ◽

Geletaw Asemahegn ◽

Habtamu Geleta ◽

Aregawi Yalew ◽

...

Keyword(s):

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Sodium Citrate ◽

Trisodium Citrate ◽

Northwest Ethiopia ◽

Cross Sectional ◽

Erythrocyte Sedimentation ◽

Significant Difference ◽

The Mean ◽

The University

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A comparison of erythrocyte sedimentation rate between blood anticoagulated with sodium citrate and EDTA among Tuberculosis suspected patients at University of Gondar Hospital, North West Ethiopia

10.21203/rs.2.16918/v1 ◽

2019 ◽

Author(s):

Zegeye Getaneh ◽

Fekadu Ayelgn ◽

Geletaw Asemahegn ◽

Habtamu Geleta ◽

Aregawi Yalew ◽

...

Keyword(s):

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Sodium Citrate ◽

Northwest Ethiopia ◽

Cross Sectional ◽

North West ◽

Erythrocyte Sedimentation ◽

Significant Difference ◽

The Mean ◽

Study Participants

Abstract Objective: The main aim of this study was comparison of erythrocyte sedimentation rate results of sodium citrate and ethylene diamine tetra-acetic acid anticoagulant. A comparative cross-sectional study was conducted at University of Gondar Referral Hospital, Northwest, Ethiopia. A total of 70 TB suspected study participants were recruited. From each study participants, 3ml blood was collected in to EDTA tube and 1.6ml blood was collected in to a test tube containing 0.4ml Sodium Citrate anticoagulant. Results: The mean ± SD values for ESR were 57.9 ± 41.45 in EDTA and 50.99 ± 43.5 in citrate anticoagulated blood. The mean difference of ESR values between EDTA and citrated blood was 6.91 ± 13.66 mm/hr with a significant difference. The mean ± SD of ESR for EDTA and citrate blood was 59.57 ± 42.31 and 54.71 ± 40.44 mm/hr for males and 53.57 ± 44.61 and 46.04 ± 41.82 mm/hr for females, respectively. This study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants.

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Association of Inflammation With Markers of Atherogenicity in Subjects With New-Onset Atria Fibrillation in a Nigerian Community

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqz116.000 ◽

2019 ◽

Vol 152 (Supplement_1) ◽

pp. S80-S80

Author(s):

Constance Nwadike ◽

Amarachi Nwakamma ◽

Harrison Nwanjo ◽

Denis Nwosu ◽

Roy Ezekwe

Keyword(s):

Platelet Count ◽

Prothrombin Time ◽

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Serum Lipids ◽

High Density Lipoproteins ◽

Mean Values ◽

Erythrocyte Sedimentation ◽

The Mean ◽

New Onset

Abstract Objectives The evidence supporting the role of circulating markers of inflammation in the pathogenesis of atria fibrillation remains controversial. This study investigated the levels of serum lipids, C-reactive proteins, erythrocyte sedimentation rate, prothrombin time, and platelet count in subjects with new-onset atria fibrillation (NAF), but with no established cardiac disease, in a Nigerian community. Methods The case control study involved a total of 200 subjects: 110 subjects with NAF and 90 apparently healthy subjects without AF, as control. AF was confirmed by a 12-lead electrocardiogram (ECG), while excluding subjects with high risk. Blood samples from the subjects were analyzed for the following parameters: C-reactive proteins using ELISA method and serum lipids using an enzymatic, colorimetric method; erythrocyte sedimentation rate, by Westergreen method; platelet count using a Midray hematology auto analyzer; and the prothrombin time using a tissue thromboplastin method. Data collected from the result were analyzed using SPSS version 15. Results There was a significant increase in the levels of C-reactive proteins and ESR in subjects with NAF when compared with the control subjects. A significant increase was observed in the mean values of total cholesterol and low-density lipoproteins in subjects with NAF, while that of high-density lipoproteins was lower in NAF than in controls. There was no significant difference in the mean values of triglyceride in NAF and the control. Results also showed significantly reduced prothrombin time, with more platelet count in subjects with NAF. The result demonstrated that females (56.3%) had greater risk of developing AF than males (45.8%), while incidence of AF was observed to increase with age. Conclusion The results of the markers assayed suggest marked dyslipidemia, inflammation, and thrombogenesis in subjects with new-onset AF. Hence dyslipidemia and inflammation play critical roles in the pathogenesis of atria fibrillation.

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IVIG Effects on Erythrocyte Sedimentation Rate in Children

International Journal of Pediatrics ◽

10.1155/2014/981465 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 5

Author(s):

Farhad Salehzadeh ◽

Ahmadvand Noshin ◽

Sepideh Jahangiri

Keyword(s):

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Age Groups ◽

The Body ◽

Response To Treatment ◽

Erythrocyte Sedimentation ◽

Neonatal Group ◽

The Mean ◽

Laboratory Tool ◽

To Receive

Background. Erythrocyte sedimentation rate (ESR) is a valuable laboratory tool in evaluation of infectious, inflammatory, and malignant diseases. Red blood cells in outside from the body precipitate due to their higher density than the plasma. In this study we discuss the IVIG effect on ESR in different diseases and different ages.Methods and Materials. Fifty patients under 12 years old who had indication to receive IVIG enrolled in this study. Total dose of IVIG was 2 gr/kg (400 mg/kg in five days or 2 gr/kg in single dose). ESR before infusion of IVIG and within 24 hours after administration of the last dose of IVIG was checked.Results. 23 (46%) patients were males and 27 (54%) were females. The mean of ESR before IVIG was31.8±29.04and after IVIG it was47.2±36.9; this difference was meaningful(P=0.05). Results of ESR changes in different age groups, 6 patients less than 28 days, 13 patients from 1 month to 1 year, 20 patients from 1 to 6 years old, and 11 patients from 6 to 12 years have been meaningful (P=0.001,P=0.025, andP=0.006, resp.).Conclusion. In patients who are receiving IVIG as a therapy, ESR increased falsely (noninflammatory rising); therefore use of ESR for monitoring of response to treatment may be unreliable. Although these results do not apply to neonatal group, we suggest that, in patients who received IVIG, interpretation of ESR should be used cautiously on followup.

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Should Ultrasonographic Giant-Cell Arteritis Signs be Detected in Patients with Polymyalgia Rheumatica?

Medical Laboratory Technology Journal ◽

10.31964/mltj.v0i0.344 ◽

2021 ◽

Author(s):

Fazıl Kulaklı ◽

İlker Fatih Sari ◽

Erdem Çaylı ◽

Nurçe Çilesizoğlu Yavuz

Keyword(s):

Giant Cell Arteritis ◽

Giant Cell ◽

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Polymyalgia Rheumatica ◽

Control Group ◽

Newly Diagnosed ◽

Erythrocyte Sedimentation ◽

Experienced Radiologist ◽

Significant Difference

Assessing the presence of ultrasonographic findings of Giant-Cell Arteritis in Polymyalgia Rheumatica patients using Ultrasonography on the temporal artery. The study contributes to the literature evaluating the unclear relationship between Polymyalgia Rheumatica and Giant-Cell Arteritis. It raises awareness that Ultrasonography can be used instead of biopsy in patients with suspected Giant-Cell Arteritis. Twenty patients were newly diagnosed with Polymyalgia Rheumatica, and 20 participants as a control group were included in the study. While the Polymyalgia Rheumatica group was evaluated at baseline and sixth month, the control group was evaluated only at baseline. Laboratory, clinical and ultrasonographic findings of all participants were assessed. Gray-scale Ultrasonography and colored Doppler Ultrasonography were used to present halo and compression, occlusion, and stenosis in addition to intima-media complex thickness in bilateral temporal arteries and frontal-parietal branches by an experienced radiologist blinded to the subject. No significant difference was found between Polymyalgia Rheumatica and control groups based on demographic features, clinical and ultrasonographic results at baseline and sixth month. Erythrocyte sedimentation rate of Polymyalgia Rheumatica at baseline was statistically higher than the control group. Erythrocyte sedimentation rate values have declined significantly in Polymyalgia Rheumatica patients, but no significant changes were found for clinical or ultrasonographic features during the sixth month. Ultrasonographic findings of Giant-Cell Arteritis are not present in newly diagnosed and six months followed up Polymyalgia Rheumatica patients. Further studies are needed.

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A Comparison Between Erythrocyte Sedimentation Rate (ESR) and Selected Acute-Phase Proteins in the Elderly

American Journal of Clinical Pathology ◽

10.1093/ajcp/94.5.637 ◽

1990 ◽

Vol 94 (5) ◽

pp. 637-640 ◽

Cited By ~ 15

Author(s):

Paul R. Katz ◽

Jurgis Karuza ◽

Steven I. Gutman ◽

William Bartholomew ◽

Gary Richman

Keyword(s):

Erythrocyte Sedimentation Rate ◽

Acute Phase ◽

Sedimentation Rate ◽

Acute Phase Proteins ◽

The Elderly ◽

Erythrocyte Sedimentation

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Comparative Analysis of Erythrocyte Sedimentation Rate Measured by Automated and Manual Methods in Anaemic Patients

Journal of Laboratory Physicians ◽

10.1055/s-0040-1721155 ◽

2020 ◽

Vol 12 (04) ◽

pp. 239-243

Author(s):

Vikram Narang ◽

Sumit Grover ◽

Amandeep Kaur Kang ◽

Avantika Garg ◽

Neena Sood

Keyword(s):

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Manual Measurement ◽

Automated Method ◽

Erythrocyte Sedimentation ◽

Manual Methods ◽

Automated Instrument ◽

Very High ◽

Edta Blood ◽

High Range

Abstract Purpose Erythrocyte sedimentation rate (ESR) is a widely used indicator of inflammation and a routinely done hematology investigation to monitor patients of autoimmune and infectious diseases. We aimed to compare the ESR results obtained by Roller 20LC automated instrument and standard reference Westergren method and analyzed the effect of anemia (hematocrit) on ESR measurements through the automated method. Methods We analyzed 1377 random anemic OPD patients (hematocrit [HCT] < 35%) for ESR levels measured by Roller 20LC using EDTA blood and Westergren method using citrated blood for a one and half year period from January 1, 2018 to June 30, 2019. Fabry’s formula was used to correct the Westergren ESR. Results The total number of samples after evaluation were divided into low (n = 232), intermediate (n = 417), high (n = 406), and very high range of ESR (≥100 mm/hr; n = 422). Mean difference between values of corrected and automated ESR for the low, intermediate, high and very high ESR range was 2.33 ± 5.03, 10.95 ± 8.04, 28.22 ± 19.11 and 43.3 ± 19.22 mm/hr, respectively. The 95% limit of agreement calculated by the Bland–Altmann analysis between the two methods for low-ESR range was −7.53 to 12.2 (highest correlation coefficient –0.65), while for very high ESR, range was −5.1 to 81.5 (least coefficient of 0.18) (p < 0.001). Conclusion In laboratories with high-sample load and where manual measurement may be tedious, the automated method of ESR measurement can safely replace the Westergren method for low-ESR values in patients with low hematocrit. While for high-ESR values, validation by the standard Westergren method may be needed.

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